{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T10:03:33Z","timestamp":1779789813162,"version":"3.53.1"},"reference-count":31,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation Project of Chongqing","award":["cstc2020jcyj-msxm-X0141"],"award-info":[{"award-number":["cstc2020jcyj-msxm-X0141"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>A single nucleotide variant in mitochondrial DNA (mtDNA) 1555A&gt;G is associated with drug-induced hearing loss. For the 1555A&gt;G mutation site, 1555A wild-type and 1555G mutant-type plasmids were constructed, respectively. In this study, a PCR method based on the TaqMan amplification refractory mutation system was proposed to detect mtDNA 1555A&gt;G. A common upstream primer, a common TaqMan probe, and two downstream allele-specific primers with mismatched bases were designed. One-step amplification and detection of the wild-type and mutant type at the 1555 site were realized for the deafness-related gene through two reactions. Based on this detection method, the minimum detection limit of the wild-type and mutant type detection systems for plasmids was 50 copies\/\u03bcL. The minimum sensitivity for the detection of nucleic acids in real dried blood spot (DBS) samples was 0.1 ng\/\u03bcL. In the normal DBS DNA sample, the detection limit of the mutation abundance reached 0.78%. The specificity of the detection method was 100%, and the coefficient of variation was less than 3.36%. This approach was validated using clinical DNA extracted from 113 DBS samples of newborns. Additionally, it showed 100% agreement with bi-directional Sanger sequencing. It can be used as an optional method for the clinical detection of deafness-related genes.<\/jats:p>","DOI":"10.3390\/cimb46060326","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T12:29:47Z","timestamp":1717417787000},"page":"5454-5466","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Application of the TaqMan ARMS-PCR Approach for Genotyping Drug-Induced Hearing Loss Using Dried Blood Samples"],"prefix":"10.3390","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3124-1113","authenticated-orcid":false,"given":"Jiefeng","family":"Tan","sequence":"first","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education of China, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education of China, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education of China, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education of China, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"242A","DOI":"10.2471\/BLT.21.285643","article-title":"The world report on hearing, 2021","volume":"99","author":"Chadha","year":"2021","journal-title":"Bull. 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