{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T04:37:15Z","timestamp":1772080635845,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Carol Davila University of Medicine and Pharmacy, Bucharest, Romania"},{"name":"Interdisziplin\u00e4res Zentrum f\u00fcr Klinische Forschung (IZKF) Jena"},{"name":"Foundation \u201cElse Kr\u00f6ner-Fresenius-Stiftung\u201d within the Else Kr\u00f6ner Research School for Physicians \u201cAntiAge\u201d"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Perinatal asphyxia (PA) and hypoxic-ischemic encephalopathy can result in severe, long-lasting neurological deficits. In vitro models, such as oxygen\u2013glucose deprivation (OGD), are used experimentally to investigate neuronal response to metabolic stress. However, multiple variables can affect the severity level of OGD\/PA and may confound any measured treatment effect. Oxytocin (OXT) has emerged as a potential neuroprotective agent against the deleterious effects of PA. Previous studies have demonstrated OXT\u2019s potential to enhance neuronal survival in immature hippocampal cultures exposed to OGD, possibly by modulating gamma-aminobutyric acid-A receptor activity. Moreover, OXT\u2019s precise impact on developing hippocampal neurons under different severities of OGD\/PA remains uncertain. In this study, we investigated the effects of OXT (0.1 \u00b5M and 1 \u00b5M) on 7-day-old primary rat hippocampal cultures subjected to 2 h OGD\/sham normoxic conditions. Cell culture viability was determined using the resazurin assay. Our results indicate that the efficacy of 1 \u00b5M OXT treatment varied according to the severity of the OGD-induced lesion, exhibiting a protective effect (p = 0.022) only when cellular viability dropped below 49.41% in non-treated OGD cultures compared to normoxic ones. Furthermore, administration of 0.1 \u00b5M OXT did not yield significant effects, irrespective of lesion severity (p &gt; 0.05). These findings suggest that 1 \u00b5M OXT treatment during OGD confers neuroprotection exclusively in severe lesions in hippocampal neurons after 7 days in vitro. Further research is warranted to elucidate the mechanisms involved in OXT-mediated neuroprotection.<\/jats:p>","DOI":"10.3390\/cimb46060371","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T12:27:33Z","timestamp":1718972853000},"page":"6223-6236","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Oxytocin Exhibits Neuroprotective Effects on Hippocampal Cultures under Severe Oxygen\u2013Glucose Deprivation Conditions"],"prefix":"10.3390","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9766-1558","authenticated-orcid":false,"given":"Mara Ioana","family":"Ionescu","sequence":"first","affiliation":[{"name":"Department of Functional Sciences, Division of Physiology II-Neuroscience, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5430-7919","authenticated-orcid":false,"given":"Ioana-Florentina","family":"Grigoras","sequence":"additional","affiliation":[{"name":"Department of Functional Sciences, Division of Physiology II-Neuroscience, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"},{"name":"Wellcome Centre for Integrative Neuroimaging, Functional MRI of the Brain, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford OX3 9DU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosana-Bristena","family":"Ionescu","sequence":"additional","affiliation":[{"name":"Department of Functional Sciences, Division of Physiology II-Neuroscience, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"},{"name":"Department of Clinical Neurosciences, University of Cambridge, Cambridge CB2 1TN, UK"},{"name":"NIHR Biomedical Research Centre, University of Cambridge, Cambridge CB2 0QQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3241-2782","authenticated-orcid":false,"given":"Diana Maria","family":"Chitimus","sequence":"additional","affiliation":[{"name":"Department of Functional Sciences, Division of Physiology II-Neuroscience, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1039-6005","authenticated-orcid":false,"given":"Robert Mihai","family":"Haret","sequence":"additional","affiliation":[{"name":"Department of Functional Sciences, Division of Physiology II-Neuroscience, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"},{"name":"Department of Ophthalmology, University Medical Center Gottingen, 37075 Gottingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0705-4901","authenticated-orcid":false,"given":"Bogdan","family":"Ianosi","sequence":"additional","affiliation":[{"name":"Department of Functional Sciences, Division of Physiology II-Neuroscience, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"},{"name":"Department of Neurology, Stroke Unit, Neuromed Campus, Kepler University Hospital, 4020 Linz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9627-3991","authenticated-orcid":false,"given":"Mihai","family":"Ceanga","sequence":"additional","affiliation":[{"name":"Department of Functional Sciences, Division of Physiology II-Neuroscience, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"},{"name":"Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, 07747 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4302-629X","authenticated-orcid":false,"given":"Ana-Maria","family":"Zagrean","sequence":"additional","affiliation":[{"name":"Department of Functional Sciences, Division of Physiology II-Neuroscience, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"ref_1","unstructured":"Gillam-Krakauer, M., and Gowen, C.W. 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