{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T08:11:20Z","timestamp":1776759080100,"version":"3.51.2"},"reference-count":63,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T00:00:00Z","timestamp":1720828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2022R1C1C1004636"],"award-info":[{"award-number":["NRF-2022R1C1C1004636"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["2022R1C1C100636712"],"award-info":[{"award-number":["2022R1C1C100636712"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["RS-2023-00301914"],"award-info":[{"award-number":["RS-2023-00301914"]}]},{"name":"Global Learning and Academic research institution","award":["NRF-2022R1C1C1004636"],"award-info":[{"award-number":["NRF-2022R1C1C1004636"]}]},{"name":"Global Learning and Academic research institution","award":["2022R1C1C100636712"],"award-info":[{"award-number":["2022R1C1C100636712"]}]},{"name":"Global Learning and Academic research institution","award":["RS-2023-00301914"],"award-info":[{"award-number":["RS-2023-00301914"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Acanthoic acid, a diterpene isolated from the root bark of Acanthopanax koreanum Nakai, possesses diverse pharmacological activities, including anti-inflammatory, anti-diabetic, gastrointestinal protection, and cardiovascular protection. This study is the first to investigate the egg-hatching rates of Drosophila melanogaster affected by acanthoic acid. Notably, male flies supplemented with 10 \u03bcM acanthoic acid exhibited a strong increase in hatching rates compared with controls under adverse temperature conditions, suggesting a potential protective effect against environmental stressors. Molecular docking simulations revealed the binding affinities and specific interactions between acanthoic acid and proteins related to male infertility, including SHBG, ADAM17, and DNase I, with binding affinity values of \u221210.2, \u22126.8, and \u22125.8 kcal\/mol, respectively. Following the docking studies, molecular dynamic simulations were conducted for a duration of 100 ns to examine the stability of these interactions. Additionally, a total binding energy analysis and decomposition analysis offered insights into the underlying energetic components and identified key contributing residues.<\/jats:p>","DOI":"10.3390\/cimb46070440","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T16:55:36Z","timestamp":1721321736000},"page":"7411-7429","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessing the Efficacy of Acanthoic Acid Isolated from Acanthopanax koreanum Nakai in Male Infertility: An In Vivo and In Silico Approach"],"prefix":"10.3390","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1715-4455","authenticated-orcid":false,"given":"Nguyen Viet","family":"Phong","sequence":"first","affiliation":[{"name":"Department of Biology Education, Teachers College and Institute for Phylogenomics and Evolution, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyo-Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea"},{"name":"School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Republic of Korea"},{"name":"KNU-G LAMP Project Group, KNU-Institute of Basic Sciences, School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4589-2052","authenticated-orcid":false,"given":"Hyun-Jung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Plant Life and Resource Science, Sangji University, Wonju-si 26339, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunbyul","family":"Yeom","sequence":"additional","affiliation":[{"name":"School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea"},{"name":"School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Republic of Korea"},{"name":"KNU-G LAMP Project Group, KNU-Institute of Basic Sciences, School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5248-1374","authenticated-orcid":false,"given":"Seo Young","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biology Education, Teachers College and Institute for Phylogenomics and Evolution, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jahangeer, M., Mustafa, G., Munir, N., Ahmed, S., and Al-Anazi, K.M. 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