{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T16:15:18Z","timestamp":1792167318154,"version":"4.4.0"},"reference-count":34,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T00:00:00Z","timestamp":1722816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation","award":["23-26-00158"],"award-info":[{"award-number":["23-26-00158"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>In this study, the effects of different combinations of the genes Vegf, Ang, and Gdnf injected both using direct virus-mediated injection (adenovirus, Ad5) and umbilical cord blood mononuclear cells (UCBCs) on the processes of stimulation of post-ischemic innervation, angiogenesis, and regeneration in skeletal muscle were investigated in a rat hindlimb chronic ischemia model. It was shown that more pronounced stimulation of angiogenesis and restoration of post-ischemic innervation were achieved both in the early (28 days post-ischemia, dpi) and late (42 dpi) terms of the experiment in the calf muscle when UCBCs delivered the combination of Ad5-Vegf and Ad5-Ang compared to the direct injection of the same vector combination into the area of ischemia. At the same time, the inclusion of Ad5-Gdnf in the combination of Ad5-Vegf and Ad5-Ang directly injected or administered by UCBCs provided a significant increase in the number of centronuclear muscle fibers, indicating stimulation of post-ischemic reparative myogenesis. This study allowed us to determine the most effective gene combinations for angiogenesis and neurogenesis, which, in the future, may serve as the basis for the development of gene and gene cell products for the treatment of chronic lower limb ischemia.<\/jats:p>","DOI":"10.3390\/cimb46080507","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T15:24:16Z","timestamp":1722957856000},"page":"8611-8626","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Combined Delivery of the Vegf, Ang, and Gdnf Genes Stimulates Angiogenesis and Improves Post-Ischemic Innervation and Regeneration in Skeletal Muscle"],"prefix":"10.3390","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6836-7285","authenticated-orcid":false,"given":"Igor Valerievich","family":"Samatoshenkov","sequence":"first","affiliation":[{"name":"Medizinische Hochschule Brandenburg Theodor Fontane, 14770 Brandenburg an der Havel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander Maazovich","family":"Aimaletdinov","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena Yurievna","family":"Zakirova","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri Alexandrovich","family":"Chelyshev","sequence":"additional","affiliation":[{"name":"Department of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Maratovna","family":"Samatoshenkova","sequence":"additional","affiliation":[{"name":"Republican Clinical Hospital, 420138 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marat Salimovich","family":"Kadyrov","sequence":"additional","affiliation":[{"name":"Limited Liability Company \u201cAngiolife\u201d, 420015 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny Alekseevich","family":"Kniazev","sequence":"additional","affiliation":[{"name":"Artemed Fachklinik, 32545 Bad Oeynhausen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bulat Ilgamovich","family":"Salakhov","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular and Endovascular Surgery, Kazan State Medical University, 420012 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yana Olegovna","family":"Mukhamedshina","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia"},{"name":"Department of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.23736\/S0392-9590.18.03996-2","article-title":"Epidemiology of peripheral artery disease in Europe: VAS Educational Paper","volume":"37","author":"Olinic","year":"2018","journal-title":"Int. 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