{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T06:52:34Z","timestamp":1773471154455,"version":"3.50.1"},"reference-count":50,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T00:00:00Z","timestamp":1731888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sichuan Provincial Natural Foundation","award":["2023NSFSC0228"],"award-info":[{"award-number":["2023NSFSC0228"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Cadmium (Cd) exposure can induce follicular atresia and laying performance reduction in hens, which is linked to autophagy within the granulosa cells. Selenium (Se) can influence autophagy and counteract Cd toxicity. This study aimed to investigate the protective effect of Se on Cd-induced follicular atresia in laying hens. Sixty-four laying hens were randomly allocated into 4 treatments: control group: basal diet; Se group: basal diet + 0.4 mg\/kg Se from selenized yeast; Cd group: basal diet + 25 mg\/kg Cd from CdCl2; and Cd+Se group: basal diet + 25 mg\/kg Cd + 0.4 mg\/kg Se. Compared to the Cd group, Se supplementation alleviated the ovarian pathological changes and oxidative stress in the follicles, serum, liver, and ovary, increased daily laying production, ovarian weight and F5\u2013F1 follicle amounts, serum levels of progesterone and oestradiol, and up-regulated mTOR expression (p &lt; 0.05), while decreasing the count of autophagic vacuoles, ovarian atresia follicle numbers, and Cd deposition, and down-regulated expression levels of autophagy-related mRNAs, including ATG5, LC3-I, and LC3-II, Beclin1, and Dynein in the follicles (p &lt; 0.05). In conclusion, 0.4 mg\/kg Se supplementation protected against Cd-induced laying performance reduction and follicular atresia, which were achieved via decreasing oxidative stress and inhibiting mTOR pathways of autophagy.<\/jats:p>","DOI":"10.3390\/cimb46110782","type":"journal-article","created":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T06:57:54Z","timestamp":1732172274000},"page":"13119-13130","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Selenized Yeast Protects Against Cadmium-Induced Follicular Atresia in Laying Hens by Reducing Autophagy in Granulosa Cells"],"prefix":"10.3390","volume":"46","author":[{"given":"Caimei","family":"Wu","sequence":"first","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxuan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziyun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5775-1900","authenticated-orcid":false,"given":"Shiping","family":"Bai","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fali","family":"Wu","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6596-8771","authenticated-orcid":false,"given":"Jianping","family":"Wang","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6009-303X","authenticated-orcid":false,"given":"Yang","family":"Lyu","sequence":"additional","affiliation":[{"name":"Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Sichuan Province, Ministry of Education, Ministry of Agriculture and Rural Affairs, Chengdu 611130, 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(2020). The effects of cadmium toxicity. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17113782"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.cotox.2019.12.002","article-title":"An overview of molecular mechanisms in cadmium toxicity","volume":"19","author":"Baralic","year":"2020","journal-title":"Curr. Opin. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"324","DOI":"10.3109\/01913123.2015.1027436","article-title":"Effect of Cadmium on Cellular Ultrastructure in Mouse Ovary","volume":"39","author":"Wang","year":"2015","journal-title":"Ultrastruct. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.toxlet.2008.07.016","article-title":"Cadmium exerts toxic effects on ovarian steroid hormone release in rats","volume":"182","author":"Zhang","year":"2008","journal-title":"Toxicol. Lett."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"39041","DOI":"10.1007\/s11356-021-18405-2","article-title":"Transcriptomics and transmission ultrastructural examination reveals the nephrotoxicity of cadmium in laying hens","volume":"29","author":"Zhao","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1093\/ps\/81.3.408","article-title":"Changes in the Populations of Mitotic and Apoptotic Cells in White Follicles during Atresia in Hens","volume":"81","author":"Kitamura","year":"2002","journal-title":"Poult. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s12011-012-9343-7","article-title":"Ovarian toxicity induced by dietary cadmium in hen","volume":"148","author":"Yang","year":"2012","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/s12011-018-1341-y","article-title":"Alleviation Mechanisms of Selenium on Cadmium-Spiked in Chicken Ovarian Tissue: Perspectives from Autophagy and Energy Metabolism","volume":"186","author":"Wang","year":"2018","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"33103","DOI":"10.1007\/s11356-020-09496-4","article-title":"Effects of dietary cadmium supplementation on production performance, cadmium residue in eggs, and hepatic damage in laying hens","volume":"27","author":"Tao","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1080\/00439339.2020.1729669","article-title":"Evaluation of dietary presence or use of cadmium in poultry","volume":"76","author":"Olgun","year":"2020","journal-title":"World Poult. Sci. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.jhazmat.2012.08.012","article-title":"Selenium reduces cadmium uptake and mitigates cadmium toxicity in rice","volume":"235","author":"Lin","year":"2012","journal-title":"J. Hazard. Mater."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1007\/s12011-017-1125-9","article-title":"Effects of Se and Cd co-treatment on the morphology, oxidative stress, and ion concentrations in the ovaries of laying hens","volume":"183","author":"Li","year":"2018","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.tox.2007.09.001","article-title":"The hepatoprotective effects of selenium against cadmium toxicity in rats","volume":"242","author":"Newairy","year":"2007","journal-title":"Toxicology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s12011-017-1193-x","article-title":"Ameliorative Effects of Selenium on Cadmium-Induced Injury in the Chicken Ovary: Mechanisms of Oxidative Stress and Endoplasmic Reticulum Stress in Cadmium-Induced Apoptosis","volume":"184","author":"Wan","year":"2018","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1007\/s12011-009-8324-y","article-title":"Protective effects of selenium, zinc, or their combination on cadmium-induced oxidative stress in rat kidney","volume":"130","author":"Messaoudi","year":"2009","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.fct.2013.04.013","article-title":"The protective effects of selenium on cadmium-induced oxidative stress and apoptosis via mitochondria pathway in mice kidney","volume":"58","author":"Wang","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1080\/1828051X.2016.1222247","article-title":"Impact of selenium supplementation on productive performance and egg selenium status in native Aseel chicken","volume":"15","author":"Zia","year":"2016","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1093\/eurjhf\/hfr167","article-title":"Selenium- and zinc-deficient cardiomyopathy in human intestinal malabsorption: Preliminary results of selenium\/zinc infusion","volume":"14","author":"Frustaci","year":"2012","journal-title":"Eur. J. Heart Fail."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/s12011-017-1186-9","article-title":"The protective effect of selenium on the chicken pancreas against cadmium toxicity via alleviating oxidative stress and autophagy","volume":"184","author":"Liu","year":"2018","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_21","unstructured":"National Research Council (1994). Nutrient Requirements of Poultry, National Academies Press. [9th ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wu, C., Song, J., Li, L., Jiang, Y., Applegate, T.J., Wu, B., Liu, G., Wang, J., Lin, Y., and Zhang, K. (2022). Protective Effects of Selenized Yeast on the Combination of Cadmium, Lead, Mercury, and Chromium-induced Toxicity in Laying Hens. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.958056"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wang, J., Jia, R., Gong, H., Celi, P., Zhuo, Y., Ding, X., Bai, S., Zeng, Q., Yin, H., and Xu, S. (2021). The effect of oxidative stress on the chicken ovary: Involvement of microbiota and melatonin interventions. Antioxidants, 10.","DOI":"10.3390\/antiox10091422"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.ebiom.2019.02.020","article-title":"Fibroblast growth factor 21 coordinates adiponectin to mediate the beneficial effects of low-protein diet on primordial follicle reserve","volume":"41","author":"Zhuo","year":"2019","journal-title":"EBioMedicine"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Chen, X., Sun, X., Chimbaka, I.M., Qin, N., Xu, X., Liswaniso, S., Xu, R., and Gonzalez, J.M. (2021). Transcriptome analysis of ovarian follicles reveals potential pivotal genes associated with increased and decreased rates of chicken egg production. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.622751"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"He, H., Li, D., Tian, Y., Wei, Q., Amevor, F.K., Sun, C., Yu, C., Yang, C., Du, H., and Jiang, X. (2022). miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1. J. Anim. Sci. Biotechnol., 13.","DOI":"10.1186\/s40104-022-00697-0"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Massanyi, P., Massanyi, M., Madeddu, R., Stawarz, R., and Lukac, N. (2020). Effects of cadmium, lead, and mercury on the structure and function of reproductive organs. Toxics, 8.","DOI":"10.3390\/toxics8040094"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zheng, Y., Qiu, Y., Wang, Q., Gao, M., Cao, Z., and Luan, X. (2024). ADPN Regulates Oxidative Stress-Induced Follicular Atresia in Geese by Modulating Granulosa Cell Apoptosis and Autophagy. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25105400"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"101524","DOI":"10.1016\/j.psj.2021.101524","article-title":"Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken","volume":"101","author":"Han","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.chemosphere.2017.12.026","article-title":"Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis","volume":"194","author":"Chen","year":"2018","journal-title":"Chemosphere"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cellsig.2018.12.008","article-title":"Cadmium results in accumulation of autophagosomes-dependent apoptosis through activating Akt-impaired autophagic flux in neuronal cells","volume":"55","author":"Zhang","year":"2019","journal-title":"Cell. Signal."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.chemosphere.2018.10.019","article-title":"Cadmium-mediated miR-30a-GRP78 leads to JNK-dependent autophagy in chicken kidney","volume":"215","author":"Shi","year":"2019","journal-title":"Chemosphere"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1002\/tox.23823","article-title":"Cadmium accelerates autophagy of osteocytes by inhibiting the PI3K\/AKT\/mTOR signaling pathway","volume":"38","author":"Song","year":"2023","journal-title":"Environ. Toxicol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.anireprosci.2017.08.008","article-title":"ROS activates autophagy in follicular granulosa cells via mTOR pathway to regulate broodiness in goose","volume":"185","author":"Lou","year":"2012","journal-title":"Anim. Reprod. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1080\/15548627.2015.1106668","article-title":"MTOR, PIK3C3, and autophagy: Signaling the beginning from the end","volume":"11","author":"Munson","year":"2015","journal-title":"Autophagy"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1080\/00984109708984008","article-title":"Restriction of cadmium transfer to eggs from laying hens exposed to cadmium","volume":"51","author":"Sato","year":"1997","journal-title":"J. Toxicol. Environ. Health"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/bs.vh.2018.01.013","article-title":"The effect of steroid hormones on ovarian follicle development","volume":"107","author":"Chou","year":"2018","journal-title":"Vitam. Horm."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"383","DOI":"10.33549\/physiolres.930408","article-title":"Components of cigarette smoke inhibit expansion of oocyte-cumulus complexes from porcine follicles","volume":"52","author":"Vrsanska","year":"2003","journal-title":"Physiol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"A255","DOI":"10.1096\/fasebj.21.5.A255-b","article-title":"Cadmium chloride and sodium arsenate, environmental estrogens in cigarette smoke, activate estrogen signaling pathways to induce proliferation in a human lung adenocarcinoma cell line","volume":"21","author":"Huff","year":"2007","journal-title":"FASEB J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/s41419-020-2246-1","article-title":"Cross-talk between autophagy and apoptosis regulates testicular injury\/recovery induced by cadmium via PI3K with mTOR-independent pathway","volume":"11","author":"Wang","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anifeedsci.2014.02.005","article-title":"Selenium in poultry breeder nutrition: An update","volume":"191","author":"Surai","year":"2014","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"597","DOI":"10.5455\/javar.2020.g457","article-title":"Dietary supplementation of nano-selenium improves reproductive performance, sexual behavior and deposition of selenium in the testis and ovary of Japanese quail","volume":"7","author":"Hafez","year":"2020","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s12011-016-0752-x","article-title":"Role of Hydrogen Sulfide on Autophagy in Liver Injuries Induced by Selenium Deficiency in Chickens","volume":"175","author":"Wenzhong","year":"2017","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Paquette, M., El-Houjeiri, L., and Pause, A. (2018). mTOR pathways in cancer and autophagy. Cancers, 10.","DOI":"10.3390\/cancers10010018"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"824","DOI":"10.4161\/auto.9099","article-title":"Activation of the PI3K-Akt-mTOR signaling pathway promotes necrotic cell death via suppression of autophagy","volume":"5","author":"Wu","year":"2009","journal-title":"Autophagy"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1101\/gad.1599207","article-title":"Autophagy: Process and function","volume":"21","author":"Mizushima","year":"2007","journal-title":"Gene Dev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1007\/s12011-014-0070-0","article-title":"Cadmium-induced injury and the ameliorative effects of selenium on chicken splenic lymphocytes: Mechanisms of oxidative stress and apoptosis","volume":"160","author":"Liu","year":"2014","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1007\/s00128-002-0092-8","article-title":"Mercury speciation and relationship between mercury and selenium in liver of Galeus melastomus from the Mediterranean sea","volume":"69","author":"Storelli","year":"2002","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"14312","DOI":"10.1007\/s11356-017-9019-0","article-title":"Selenium for the mitigation of toxicity induced by lead in chicken testes through regulating mRNA expressions of HSPs and selenoproteins","volume":"24","author":"Huang","year":"2017","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"10074","DOI":"10.1002\/anie.201505056","article-title":"Small organoselenium compounds: More than just glutathione peroxidase mimics","volume":"54","author":"Wirth","year":"2015","journal-title":"Angew. Chem. Int. Ed."}],"container-title":["Current Issues in Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1467-3045\/46\/11\/782\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:34:23Z","timestamp":1760114063000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1467-3045\/46\/11\/782"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,18]]},"references-count":50,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["cimb46110782"],"URL":"https:\/\/doi.org\/10.3390\/cimb46110782","relation":{},"ISSN":["1467-3045"],"issn-type":[{"value":"1467-3045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,18]]}}}