{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:04:20Z","timestamp":1760058260105,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"S\u00e3o Paulo Research Foundation","award":["#2011\/08075-4"],"award-info":[{"award-number":["#2011\/08075-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Patients carrying Chagasic megaesophagus (CME) are at high risk for esophageal carcinoma. We aimed to investigate mutations in the TP53 in patients carrying CME. Blood samples from 114 patients with Chagas disease (CD) were used. The samples were subjected to PCR-SSCP analysis and DNA sequencing in exons 5 and 7 of the TP53 gene. We observed mutations in the exon 5 codon 184 (GAT &gt; AAT) in 14.8% of G1 (11\/74), 10% of G2 (4\/40), and 5% of G3 (2\/40). We also observed the codon 185 mutation (AGC &gt; AGG) in 14.8% of G1 (11\/74), 10% of G2 (4\/40), and 7.5% of G3 (3\/40). Regarding Exon 7, a mutation (G &gt; T) was observed in the intronic region in 2.7% of G1 (2\/74), 7.5% of G2 (3\/40), and none of G3 (0\/40). Our study showed, for the first time, simultaneous mutations at codons 184 and 185 of the TP53 gene in patients with CME and Chagasic patients without megaesophagus. More studies are needed to assess whether the simultaneous presence of mutations at codons 184 and 185 increases the risk of developing esophageal carcinoma in these patients.<\/jats:p>","DOI":"10.3390\/cimb47040229","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T03:35:28Z","timestamp":1743132928000},"page":"229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["TP53 Mutations in Chagasic Megaesophagus"],"prefix":"10.3390","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2283-8969","authenticated-orcid":false,"given":"Aparecida Perpetuo Fedossi","family":"Silveira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Imunogen\u00e9tica, Departamento de Biologia Molecular, Faculdade de Medicina de S\u00e3o Jos\u00e9 do Rio Preto (FAMERP), S\u00e3o Jos\u00e9 do Rio Preto 15090-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4904-2853","authenticated-orcid":false,"given":"Ricardo Quiterio","family":"Sartori","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias, Letras e Ci\u00eancias Exatas (IBILCE), Universidade Estadual Paulista J\u00falio de Mesquita Filho (UNESP), S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9999-2673","authenticated-orcid":false,"given":"Lilian","family":"Castiglioni","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunogen\u00e9tica, Departamento de Biologia Molecular, Faculdade de Medicina de S\u00e3o Jos\u00e9 do Rio Preto (FAMERP), S\u00e3o Jos\u00e9 do Rio Preto 15090-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2631-2104","authenticated-orcid":false,"given":"Heidi Lacerda","family":"Alves da Cruz","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopathology Keizo Asami, Universidade Federal de Pernambuco (UFPE), Recife 50670-901, PE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9983-5419","authenticated-orcid":false,"given":"Eumildo de Campos","family":"Junior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunogen\u00e9tica, Departamento de Biologia Molecular, Faculdade de Medicina de S\u00e3o Jos\u00e9 do Rio Preto (FAMERP), S\u00e3o Jos\u00e9 do Rio Preto 15090-000, SP, Brazil"},{"name":"Departamento de Cirurgia do Hospital de Base, Funda\u00e7\u00e3o Faculdade Regional de Medicina de S\u00e3o Jos\u00e9 do Rio Preto (FUNFARME), S\u00e3o Jos\u00e9 do Rio Preto 15090-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4836-3113","authenticated-orcid":false,"given":"Cinara C\u00e1ssia","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunogen\u00e9tica, Departamento de Biologia Molecular, Faculdade de Medicina de S\u00e3o Jos\u00e9 do Rio Preto (FAMERP), S\u00e3o Jos\u00e9 do Rio Preto 15090-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8572-8177","authenticated-orcid":false,"given":"Luiz Carlos","family":"de Mattos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunogen\u00e9tica, Departamento de Biologia Molecular, Faculdade de Medicina de S\u00e3o Jos\u00e9 do Rio Preto (FAMERP), S\u00e3o Jos\u00e9 do Rio Preto 15090-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"ref_1","unstructured":"(2022, June 21). 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