{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T20:16:56Z","timestamp":1767212216570,"version":"build-2065373602"},"reference-count":25,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Vietnamese Ministry of Science and Technology","award":["NVQG-2019\/\u0110T.02"],"award-info":[{"award-number":["NVQG-2019\/\u0110T.02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Neisseria meningitidis is a pathogenic bacterial agent that causes meningococcal meningitis in humans. Developing a rapid and low-cost N. meningitidis detection method is crucial, especially for developing countries. This study focuses on the development of an efficient loop-mediated isothermal amplification (LAMP) method for accurate N. meningitidis identification. A new LAMP primer set was designed, and a LAMP reaction was optimized. The colorimetric detection method was also applied, and the assay characteristics were evaluated using clinical samples. The results demonstrated a specific LAMP reaction for N. meningitidis detection of genotypes A, B, and C, with a limit of detection of 102 cfu\/mL, 100% specificity and sensitivity, and a rapid detection time of only 40 min by colorimetric visual inspection. No cross-reactivity with reference strains of Streptococcus pneumoniae, Staphylococcus aureus, Neisseria lactamica, Mycobacterium tuberculosis, and Haemophilus influenzae type b was observed in the LAMP reaction with the new primer set. This result suggests that the LAMP reaction could be a promising tool for developing a rapid N. meningitidis detection method suitable for use in Vietnam and other developing countries.<\/jats:p>","DOI":"10.3390\/cimb47060467","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T10:45:26Z","timestamp":1750157126000},"page":"467","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design of New Primer Sets for the Development of a Loop-Mediated Isothermal Amplification for Rapid Detection of Neisseria meningitidis"],"prefix":"10.3390","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5147-7085","authenticated-orcid":false,"given":"Cuong Viet","family":"Vo","sequence":"first","affiliation":[{"name":"Joint Vietnam-Russian Tropical Science and Technology Research Center, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trang Thu","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Health Science and Technology, Hanoi University of Science and Technology, Hanoi 100000, Vietnam"},{"name":"School of Chemistry and Life Sciences, Hanoi University of Science and Technology, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huong Thu","family":"Ngo","sequence":"additional","affiliation":[{"name":"Center for Research and Production of Vaccines and Biologicals, Ministry of Health, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9266-2156","authenticated-orcid":false,"given":"Lan Anh Thi","family":"Bui","sequence":"additional","affiliation":[{"name":"Joint Vietnam-Russian Tropical Science and Technology Research Center, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toan Van","family":"Trinh","sequence":"additional","affiliation":[{"name":"Joint Vietnam-Russian Tropical Science and Technology Research Center, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loan Thi","family":"Vu","sequence":"additional","affiliation":[{"name":"Joint Vietnam-Russian Tropical Science and Technology Research Center, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hieu Dang","family":"Hoang","sequence":"additional","affiliation":[{"name":"Joint Vietnam-Russian Tropical Science and Technology Research Center, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phong Quoc","family":"Truong","sequence":"additional","affiliation":[{"name":"Institute of Health Science and Technology, Hanoi University of Science and Technology, Hanoi 100000, Vietnam"},{"name":"School of Chemistry and Life Sciences, Hanoi University of Science and Technology, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Seib, K.L., and Peak, I.R. 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