{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T05:34:16Z","timestamp":1766122456218,"version":"3.48.0"},"reference-count":39,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82460157"],"award-info":[{"award-number":["82460157"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Guizhou Provincial Committee Organization Department","award":["QKHPTRC-GCC [2022]034-1"],"award-info":[{"award-number":["QKHPTRC-GCC [2022]034-1"]}]},{"name":"Guizhou Provincial Science and Technology Foundation","award":["ZK [2024] Major 030"],"award-info":[{"award-number":["ZK [2024] Major 030"]}]},{"name":"Guizhou Provincial Health Commission Science and Technology Foundation","award":["gzwkj2024-147"],"award-info":[{"award-number":["gzwkj2024-147"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Benign prostatic hyperplasia (BPH) is a common disease in elderly men; its occurrence is closely related to the interaction between stromal cells and epithelial cells in the prostate. This article aims to explore the potential therapeutic effect and mechanism of a new trifluoromethyl quazoline compound (kzl054) on BPH. The results showed that kzl054 had inhibitory activity that limited the growth of prostate hyperplasia cells, BPH-1, and stromal cells, WPMY-1. It could also induce apoptosis of BPH-1 cells and arrest their cell cycle. animal experiment results showed that kzl054 could effectively reduce the volume and prostate index of mouse prostate hyperplasia tissues. Through the establishment of a co-culture system of BPH-1 and WPMY-1 cells, it was found that co-culture could induce EMT in BPH-1 cells. While kzl054 could affect the secretion of TGF-\u03b21 by competitively binding to the colchicine binding site on \u03b2-tubulin and inhibiting the expression of \u03b2-tubulin, through inhibiting the secretion of TGF-\u03b21 by stromal cells. This study has revealed that compound kzl054 inhibits the secretion of TGF-\u03b21 by targeting the inhibition of microtubule polymerization and regulating the epithelial cell EMT, providing potential candidate molecules and mechanisms for the development of new drugs for the treatment of BPH.<\/jats:p>","DOI":"10.3390\/cimb47121057","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T10:41:23Z","timestamp":1765968083000},"page":"1057","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Trifluoromethyl Quinazoline Compound Regulates the Epithelial\u2013Mesenchymal Transition of Prostatic Hyperplasia Cells by Inhibiting the Secretion of TGF-\u03b21 in Stromal Cells"],"prefix":"10.3390","volume":"47","author":[{"given":"Lu","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guiyang 550014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sha","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guiyang 550014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bixue","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guiyang 550014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guiyang 550014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiming","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guiyang 550014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3418-5739","authenticated-orcid":false,"given":"Heng","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guiyang 550014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1159\/000496289","article-title":"Pathophysiology of Benign Prostatic Hyperplasia and Benign Prostatic Enlargement: A Mini-Review","volume":"65","author":"Madersbacher","year":"2019","journal-title":"Gerontology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.4103\/jwas.jwas_61_23","article-title":"Urological Surgeries in a West African Teaching Hospital","volume":"13","author":"Okeke","year":"2023","journal-title":"J. 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