{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T06:59:21Z","timestamp":1766991561263,"version":"3.48.0"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T00:00:00Z","timestamp":1766793600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32301618"],"award-info":[{"award-number":["32301618"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>The excessively concentrated ripening period of mulberries causes seasonal surplus. Fruit mulberry (Morus spp.) exhibits the unique trait of \u201csimultaneous flowering and leaf flushing\u201d, rendering budburst timing closely correlated with fruit ripening time. Thus, deciphering the molecular mechanism underlying winter bud dormancy maintenance in fruit mulberry is urgently needed. Herein, an F1 hybrid population comprising 337 individuals, derived from Morus wittiorum (\u2640) and \u2018322\u2019 (\u2642), was utilized as research material. Through Bulked Segregant Analysis Sequencing (BSA-Seq), we successfully mapped a dormancy-associated QTL interval designated as LB (Late Burst), spanning 9,990,001\u201311,990,000 bp on Chromosome 13. Integrating Weighted Gene Co-expression Network Analysis (WGCNA) results, MaSVP was identified as a candidate gene within this interval. Virus-induced gene silencing (VIGS) of MaSVP in winter buds of Morus wittiorum significantly accelerated budburst compared to the control, demonstrating that MaSVP represses winter bud dormancy release and plays a crucial role in regulating dormancy maintenance in fruit mulberry. Dynamic expression profiling of dormancy-related genes revealed that the transcript levels of MaSVP, MaSAPK3, MaCASL2, and MaPYR8 were significantly downregulated (Tukey\u2019s test, p &lt; 0.05) as budburst approached, whereas those of MaFT and MaGA20ox1-D were significantly upregulated (Tukey\u2019s test, p &lt; 0.05). These results indicate that winter bud dormancy maintenance in Morus wittiorum is associated with abscisic acid (ABA) and gibberellin (GA) metabolism. Collectively, this study provides critical insights into the biological basis of winter bud dormancy maintenance in fruit mulberry and offers valuable genetic resources for breeding late-maturing cultivars.<\/jats:p>","DOI":"10.3390\/cimb48010038","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T06:25:51Z","timestamp":1766989551000},"page":"38","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated BSA-Seq and WGCNA Analyses Reveal Candidate Genes Associated with Winter Bud Dormancy Maintenance in Fruit Mulberry (Morus spp.)"],"prefix":"10.3390","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0932-3474","authenticated-orcid":false,"given":"Bing","family":"Sun","sequence":"first","affiliation":[{"name":"Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China"},{"name":"Seedling Management Station of Hubei Provincial Forestry Bureau, Wuhan 430079, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoxia","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1136-8639","authenticated-orcid":false,"given":"Zhixian","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cui","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,27]]},"reference":[{"key":"ref_1","first-page":"197","article-title":"CLIMATE CHANGE TRIGGERS EARLY SPROUTING IN MULBERRY (Morus sp.)","volume":"10","author":"Mehraj","year":"2020","journal-title":"Int. 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