{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T17:10:05Z","timestamp":1767201005658,"version":"3.48.0"},"reference-count":40,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fagron BV"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Background: Functional dermatological bases can contribute more than just delivery\u2014they may actively modulate cutaneous homeostasis. Cleoderm\u2122 is a dermofunctional base containing a patented Cleome gynandra extract, palmitoyl tripeptide-8, bisabolol, hyaluronic acid, and functional oils, rationally designed to provide anti-inflammatory, antioxidant, and barrier-supportive properties. Objective: To determine whether Cleoderm\u2122 exhibits intrinsic immunomodulatory and matrix-protective effects in a physiologically relevant skin co-culture and to clarify the biomarkers most impacted, with translational relevance to acne and rosacea. Methods: Human keratinocytes and fibroblasts were maintained in a transwell co-culture. Non-cytotoxic concentrations of Cleoderm\u2122 (1.0% and 10.0%, v\/v) were tested with or without LPS stimulation (1 \u03bcg\/mL). Viability was assessed by MTT and Trypan Blue. Cytokines (IL-6, TNF-\u03b1, IL-10, TGF-\u03b2) and MMPs (MMP-1, -3, -13) were quantified by ELISA and RT-qPCR. LL-37, IGFBP-3, and TGF-\u03b2 protein levels were evaluated by Western blot. Results: Cleoderm\u2122 showed no cytotoxicity up to 10% (v\/v). It significantly reduced pro-inflammatory mediators (IL-6, TNF-\u03b1) and matrix-degrading enzymes (MMP-1, MMP-3, MMP-13) while increasing anti-inflammatory\/reparative cytokines (IL-10, TGF-\u03b2). A dual, biomarker-level modulation was observed: (i) LL-37 was reduced, with a particularly pronounced decrease in secreted levels; and (ii) IGFBP-3 was markedly upregulated, indicating potential attenuation of the IGF-1 axis relevant to sebaceous lipogenesis. Collectively, these effects indicate immunoregulatory and matrix-protective activity consistent with improved cutaneous homeostasis. Conclusion: In a dermo-epidermally relevant in vitro model, Cleoderm\u2122 functions as an active dermofunctional base, not merely a vehicle simultaneously tempering inflammatory signaling, preserving extracellular matrix integrity, and modulating mechanistic nodes (LL-37 and IGFBP-3) linked to rosacea and acne. These findings is consistent with the use of Cleoderm\u2122 as a biologically supportive base for personalized compounding and justify controlled clinical evaluation.<\/jats:p>","DOI":"10.3390\/cimb48010054","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T16:34:23Z","timestamp":1767198863000},"page":"54","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dermofunctional Vehicle Downregulates LL-37 and MMPs and Upregulates IGFBP-3"],"prefix":"10.3390","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2119-3660","authenticated-orcid":false,"given":"Hudson","family":"Polonini","sequence":"first","affiliation":[{"name":"Fagron BV, Fascinatio Boulevard 350, 3065 WB Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7590-3904","authenticated-orcid":false,"given":"Fabiana Regina","family":"da Silva Ol\u00edmpio","sequence":"additional","affiliation":[{"name":"Gap Biotech, Biotechnology Laboratory, Sao Jose dos Campos 12243-020, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8634-2850","authenticated-orcid":false,"given":"Carlos Rocha","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Gap Biotech, Biotechnology Laboratory, Sao Jose dos Campos 12243-020, Brazil"},{"name":"Postgraduate Program in Biomedical Engineering, Department of Science and Technology, Federal University of Sao Paulo (UNIFESP), Sao Jose dos Campos 12231-280, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"ref_1","first-page":"861","article-title":"The Global Burden of Skin Disease in 2010: An Analysis of the Prevalence and Impact of Skin Conditions","volume":"70","author":"Hay","year":"2014","journal-title":"J. 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