{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:19Z","timestamp":1770076819909,"version":"3.49.0"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondo Sectorial de Investigaci\u00f3n en Servicios de Salud. CONACyT","award":["2013-01-201251"],"award-info":[{"award-number":["2013-01-201251"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Pre-eclampsia is a Hypertensive Disorder of Pregnancy (HDP) characterized by hypertension and proteinuria, affecting 2\u20138% of pregnancies worldwide and constituting a major public health concern. Genes of the renin\u2013angiotensin system have been investigated as potential causative factors, but inconclusive results have been obtained. The objective of this pilot study is to evaluate the possible contribution of alleles, genotypes or haplotypes of two single-nucleotide polymorphisms (SNPs) T174M (rs4762) and M235T (rs699) in AGT gene to pre-eclampsia in the Mexican population. We analyzed the association by performing PCR-RFLP with DNA extracted from whole blood samples of Mexican women with pre-eclampsia or normotensive pregnancy and the general population (GP). Our results showed a significant difference in the rate of heterozygosity for the T174M polymorphism between cases and controls. In addition, this polymorphism together with homozygosity for the M235T polymorphism may represent a possible genetic marker associated with pre-eclampsia. The T-C haplotype (174M\u2013M235) was more common in patients with pre-eclampsia (non-significant difference p = 0.0503). The identification of genetic risk markers may support the early detection of pre-eclampsia and strengthen peripartum maternal health strategies within a global health framework aimed at reducing maternal mortality.<\/jats:p>","DOI":"10.3390\/cimb48020168","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T09:48:08Z","timestamp":1770025688000},"page":"168","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["T174M-M235T AGT Gene Haplotypes in Women with Pre-Eclampsia from Northwest Mexico: A Pilot Case-Control Study"],"prefix":"10.3390","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4902-0072","authenticated-orcid":false,"given":"Jorge H.","family":"Portillo-Gallo","sequence":"first","affiliation":[{"name":"Instituto Nacional de Aprendizaje, Habilidades e Investigaci\u00f3n de las Ciencias (INAHIC), Diana Natura H1, Zapopan ZC 45221, JA, Mexico"},{"name":"Federaci\u00f3n Mexicana de Patolog\u00eda Cl\u00ednica y Medicina de Laboratorio, Av. Cuauht\u00e9moc 330, Colonia Doctores ZC 06720, CDMX, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1942-0163","authenticated-orcid":false,"given":"Jorge Manuel","family":"S\u00e1nchez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Aprendizaje, Habilidades e Investigaci\u00f3n de las Ciencias (INAHIC), Diana Natura H1, Zapopan ZC 45221, JA, Mexico"},{"name":"Federaci\u00f3n Mexicana de Patolog\u00eda Cl\u00ednica y Medicina de Laboratorio, Av. Cuauht\u00e9moc 330, Colonia Doctores ZC 06720, CDMX, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2272-9612","authenticated-orcid":false,"given":"Ana Miriam","family":"Salda\u00f1a-Cruz","sequence":"additional","affiliation":[{"name":"Instituto de Terapia Experimental y Cl\u00ednica (INTEC), Departamento de Farmacolog\u00eda, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Colonia Independencia, Guadalajara ZC 44340, JA, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha Roc\u00edo","family":"Hern\u00e1ndez-Preciado","sequence":"additional","affiliation":[{"name":"Departamento de Disciplinas Filos\u00f3fico, Metodol\u00f3gicas e Instrumentales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 750, Puerta 1B Edificio N, Colonia Independencia, Guadalajara ZC 44340, JA, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5218-4118","authenticated-orcid":false,"given":"Luis Arturo","family":"Camacho-Silvas","sequence":"additional","affiliation":[{"name":"Facultad de Medicina y Ciencias Biom\u00e9dicas, Universidad Aut\u00f3noma de Chihuahua, Circuito Universitario 31109, Campus II, Chihuahua ZC 31512, CH, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica Michelle","family":"Ledesma-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratorio Cl\u00ednico, Hospital \u00c1ngeles Andares, Calle Universidad 200, Zapopan ZC 45116, JA, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9ctor Alfonso","family":"G\u00f3mez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Disciplinas Filos\u00f3fico, Metodol\u00f3gicas e Instrumentales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 750, Puerta 1B Edificio N, Colonia Independencia, Guadalajara ZC 44340, JA, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0244-9464","authenticated-orcid":false,"given":"Jhonathan","family":"C\u00e1rdenas-Bedoya","sequence":"additional","affiliation":[{"name":"Departamento de Disciplinas Filos\u00f3fico, Metodol\u00f3gicas e Instrumentales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 750, Puerta 1B Edificio N, Colonia Independencia, Guadalajara ZC 44340, JA, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid Patricia","family":"D\u00e1valos-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Gen\u00e9tica, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente, Delegaci\u00f3n Jalisco, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia, Guadalajara ZC 44340, JA, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Franco-Santill\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda Cl\u00ednica y Molecular, Instituto NIDIAC, Cobalto 2, Ciudad Industrial, Durango ZC 34208, DG, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6458-9617","authenticated-orcid":false,"given":"Mar\u00eda Cristina","family":"Mor\u00e1n-Moguel","sequence":"additional","affiliation":[{"name":"Federaci\u00f3n Mexicana de Patolog\u00eda Cl\u00ednica y Medicina de Laboratorio, Av. Cuauht\u00e9moc 330, Colonia Doctores ZC 06720, CDMX, Mexico"},{"name":"Departamento de Disciplinas Filos\u00f3fico, Metodol\u00f3gicas e Instrumentales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 750, Puerta 1B Edificio N, Colonia Independencia, Guadalajara ZC 44340, JA, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.ajog.2013.08.019","article-title":"Incidence of preeclampsia: Risk factors and outcomes associated with early-versus late-onset disease","volume":"209","author":"Lisonkova","year":"2013","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_2","first-page":"77","article-title":"Hypertensive Disorders of Pregnancy","volume":"39","author":"Khedagi","year":"2021","journal-title":"Cardiol. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"(2025). Global and regional causes of maternal deaths 2009\u201320: A WHO systematic analysis. Lancet Glob. Health, 13, e626\u2013e634.","DOI":"10.1016\/S2214-109X(24)00560-6"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1038\/nrneph.2014.102","article-title":"Pre-eclampsia part 1: Current understanding of its pathophysiology","volume":"10","author":"Chaiworapongsa","year":"2014","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1161\/CIRCRESAHA.118.313276","article-title":"Preeclampsia: Pathophysiology, challenges, and perspectives","volume":"124","author":"Rana","year":"2019","journal-title":"Circ. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4683798","DOI":"10.1155\/2020\/4683798","article-title":"Haplotype Analysis of Candidate Genes Involved in Inflammation and Oxidative Stress and the Susceptibility to Preeclampsia","volume":"2020","author":"Chen","year":"2020","journal-title":"J. Immunol. Res."},{"key":"ref_7","first-page":"629","article-title":"Angiotensinogen gene T174M polymorphism: Opposite relationships with essential hypertension and obesity in a homogeneous population from Majorca (Baleric Islands, Spain)","volume":"25","author":"Marco","year":"2005","journal-title":"Nefrol. Publ. Of. La Soc. Esp. Nefrol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1470320317753924","DOI":"10.1177\/1470320317753924","article-title":"Contribution of angiotensinogen M235T and T174M gene variants and haplotypes to preeclampsia and its severity in (North African) Tunisians","volume":"19","author":"Zitouni","year":"2018","journal-title":"J. Renin-Angiotensin-Aldosterone Syst."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"127452","DOI":"10.1016\/j.envpol.2025.127452","article-title":"Racial and ethnic disparities in environmental chemical exposures and hypertensive disorders of pregnancy: The ECHO-wide cohort study","volume":"392","author":"Liu","year":"2025","journal-title":"Environ. Pollut."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/sj.jhh.1001107","article-title":"Haplotypes of the human renin gene associated with essential hypertension and stroke","volume":"15","author":"Frossard","year":"2001","journal-title":"J. Hum. Hypertens."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/sj.ejhg.5200874","article-title":"Detection of putative functional angiotensinogen (AGT) gene variants controlling plasma AGT levels by combined segregation-linkage analysis","volume":"10","author":"Brand","year":"2002","journal-title":"Eur. J. Hum. Genet."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"GOPEC (2005). Disentangling fetal and maternal susceptibility for pre-eclampsia: A British multicenter candidate-gene study. Am. J. Hum. Genet., 77, 127\u2013131.","DOI":"10.1086\/431245"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11430","DOI":"10.1074\/jbc.270.19.11430","article-title":"A mutation of angiotensinogen in a patient with preeclampsia leads to altered kinetics of the renin-angiotensin system","volume":"270","author":"Inoue","year":"1995","journal-title":"J. Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.preghy.2019.08.006","article-title":"Renin-angiotensin system gene variants and risk of early- and late-onset preeclampsia: A single center case-control study","volume":"18","author":"Procopciuc","year":"2019","journal-title":"Pregnancy Hypertens."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1161\/01.HYP.0000104525.76016.77","article-title":"Implication of an AGT haplotype in a multigene association study with pregnancy hypertension","volume":"43","author":"Moutquin","year":"2004","journal-title":"Hypertension"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/s43856-024-00642-4","article-title":"Biomarkers and point of care screening approaches for the management of preeclampsia","volume":"4","author":"Ng","year":"2024","journal-title":"Commun. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1161\/HYPERTENSIONAHA.120.16422","article-title":"Real-World Use of Biomarkers in Management of Hypertension During Pregnancy: Adjunct to Clinical Care","volume":"77","author":"Suresh","year":"2021","journal-title":"Hypertension"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1161\/01.HYP.33.2.698","article-title":"Angiotensinogen gene polymorphisms M235T\/T174M","volume":"33","author":"Niu","year":"1999","journal-title":"Hypertension"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"National High Blood Pressure Education Program Working Group on High Blood Pressure in Pregnancy (2000). Report of the National High Blood Pressure Education Program Working Group on High Blood Pressure in Pregnancy. Am. J. Obstet. Gynecol., 183, S1\u2013S22.","DOI":"10.1067\/mob.2000.107928"},{"key":"ref_20","first-page":"298","article-title":"A fast method for high-quality genomic DNA extraction from whole human blood","volume":"11","author":"Gustincich","year":"1991","journal-title":"Biotechniques"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1093\/nar\/16.3.1215","article-title":"A simple salting out procedure for extracting DNA from human nucleated cells","volume":"16","author":"Miller","year":"1988","journal-title":"Nucleic Acids Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1159\/000151500","article-title":"Analysis of the association of preeclampsia with polymorphisms of the INS, INSR and IRS1 genes in Mexican women","volume":"67","author":"Perea","year":"2009","journal-title":"Gynecol. Obstet. Investig."},{"key":"ref_23","first-page":"798","article-title":"Approximation to the quality of care during pregnancy, childbirth, and postpartum in women with obstetric risk factors in Mexico","volume":"62","year":"2020","journal-title":"Salud Publica Mex."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Oancea, M., Strilciuc, S., Zanoaga, O., Ciocan, C., Malutan, A., P\u0103unescu, I., Boitor, D., Braicu, C., and Mihu, D. (2025). Trophoblast Function in Preeclampsia: Decoding the Mechanistic Roles of Coding and Non-Coding Genes. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262311709"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e001819","DOI":"10.1136\/lupus-2025-001819","article-title":"Proteomic analysis reveals angiogenesis-related plasma proteins associated with pre-eclampsia in SLE","volume":"12","author":"Torell","year":"2025","journal-title":"Lupus Sci. Med."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Wu, X., Li, X., Wu, Y., Yang, H., Wu, J., and He, L. (2025). Comprehensive identification of immune-related biomarkers and therapeutic targets in preeclampsia: Integrative bioinformatics and experimental validation. BMC Pregnancy Childbirth, 25.","DOI":"10.1186\/s12884-025-08169-9"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1161\/HYPERTENSIONAHA.116.07293","article-title":"Unsupervised placental gene expression profiling identifies clinically relevant subclasses of human preeclampsia","volume":"68","author":"Leavey","year":"2016","journal-title":"Hypertension"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e054697","DOI":"10.1136\/bmjopen-2021-054697","article-title":"Prediction of pregnancy-related hypertensive disorders using metabolomics: A systematic review","volume":"12","author":"Mayrink","year":"2022","journal-title":"BMJ Open"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0092-8674(92)90275-H","article-title":"Molecular basis of human hypertension: Role of angiotensinogen","volume":"71","author":"Jeunemaitre","year":"1992","journal-title":"Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.placenta.2025.01.005","article-title":"Association studies of vasoactive genes and preeclampsia in Taiwan","volume":"161","author":"Yang","year":"2025","journal-title":"Placenta"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3121","DOI":"10.1007\/s10815-020-01971-8","article-title":"Three polymorphisms of renin-angiotensin system and preeclampsia risk","volume":"37","author":"Wang","year":"2020","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1177\/1933719108316984","article-title":"Preeclampsia risk and angiotensinogen polymorphisms M235T and AGT-217 in African American and Caucasian women","volume":"15","author":"Jenkins","year":"2008","journal-title":"Reprod. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Vefring, H.K., Wee, L., Jugessur, A., Gjessing, H.K., Nilsen, S.T., and Lie, R.T. (2010). Maternal angiotensinogen (AGT) haplotypes, fetal renin (REN) haplotypes and risk of preeclampsia; estimation of gene-gene interaction from family-triad data. BMC Med. Genet., 11.","DOI":"10.1186\/1471-2350-11-90"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1097\/MAJ.0b013e3182121020","article-title":"Variants and haplotypes in angiotensinogen gene are associated with plasmatic angiotensinogen level in Mexican population","volume":"342","author":"Carrillo","year":"2011","journal-title":"Am. J. Med. Sci."},{"key":"ref_35","first-page":"78","article-title":"Technologies for Prediction of Preeclampsia","volume":"12","author":"Panova","year":"2021","journal-title":"Sovrem Tekhnologii Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.ecl.2024.05.012","article-title":"Update on Preeclampsia and Hypertensive Disorders of Pregnancy","volume":"53","author":"Rosenberg","year":"2024","journal-title":"Endocrinol. Metab. Clin. N. Am."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.tcm.2008.07.002","article-title":"Novel biomarkers for predicting preeclampsia","volume":"18","author":"Carty","year":"2008","journal-title":"Trends Cardiovasc. Med."}],"container-title":["Current Issues in Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1467-3045\/48\/2\/168\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T12:40:00Z","timestamp":1770036000000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1467-3045\/48\/2\/168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,2]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["cimb48020168"],"URL":"https:\/\/doi.org\/10.3390\/cimb48020168","relation":{},"ISSN":["1467-3045"],"issn-type":[{"value":"1467-3045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,2]]}}}