{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T06:12:31Z","timestamp":1773468751737,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP25am0521010"],"award-info":[{"award-number":["JP25am0521010"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP25ama121008"],"award-info":[{"award-number":["JP25ama121008"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP25ama221153"],"award-info":[{"award-number":["JP25ama221153"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP25ama221339"],"award-info":[{"award-number":["JP25ama221339"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP25bm1123027"],"award-info":[{"award-number":["JP25bm1123027"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["25K18843"],"award-info":[{"award-number":["25K18843"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["25K10553"],"award-info":[{"award-number":["25K10553"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>The type II cadherin Cadherin-19 (CDH19) plays a crucial role in neural crest development. CDH19 regulates cell\u2013cell junctions and migration by forming catenin\u2013cytoskeleton complexes. Although anti-CDH19 monoclonal antibodies (mAbs) are used for specific applications such as Western blotting and immunohistochemistry (IHC), suitable anti-CDH19 mAbs for flow cytometry are limited. Therefore, developing mAbs that specifically recognize cell-surface-expressed CDH19 is essential for advancing both basic research and therapeutic strategies. Here, novel anti-human CDH19 mAbs (Ca19Mabs) were created using flow cytometry-based high-throughput screening. One clone, Ca19Mab-8 (IgG1, \u03ba), specifically recognized CDH19-overexpressed Chinese hamster ovary-K1 cells but did not bind to other 21 CDHs (including both type I and type II CDHs) in flow cytometry. Additionally, Ca19Mab-8 recognized endogenous CDH19 in the human glioblastoma cell line LN229. The dissociation constant (KD) of Ca19Mab-8 for LN229\/CDH19 was 9.0 \u00d7 10\u22129 M. Ca19Mab-8 also detected endogenous CDH19 in Western blotting. Furthermore, Ca19Mab-8 can detect CDH19 in IHC using human melanoma tissue. These findings suggest that Ca19Mab-8 is a novel mAb that detects cell-surface-expressed CDH19 with high specificity and is suitable for various applications in basic research. Therefore, Ca19Mab-8 has potential for clinical diagnosis and tumor therapy.<\/jats:p>","DOI":"10.3390\/cimb48030307","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:12:24Z","timestamp":1773321144000},"page":"307","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Anti-Cadherin-19 Monoclonal Antibody (Ca19Mab-8) for Flow Cytometry, Western Blotting, and Immunohistochemistry"],"prefix":"10.3390","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7984-5414","authenticated-orcid":false,"given":"Guanjie","family":"Li","sequence":"first","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Miyagi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2646-5132","authenticated-orcid":false,"given":"Hiroyuki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Miyagi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4158-9208","authenticated-orcid":false,"given":"Mika K.","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Miyagi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5385-8201","authenticated-orcid":false,"given":"Yukinari","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Miyagi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1053\/j.gastro.2021.08.029","article-title":"Direct Interaction of Sox10 with Cadherin-19 Mediates Early Sacral Neural Crest Cell Migration: Implications for Enteric Nervous System Development Defects","volume":"162","author":"Huang","year":"2022","journal-title":"Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1002\/dvdy.20209","article-title":"Identification of a novel type II classical cadherin: Rat cadherin19 is expressed in the cranial ganglia and Schwann cell precursors during development","volume":"232","author":"Takahashi","year":"2005","journal-title":"Dev. Dyn."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/nrc3647","article-title":"Beyond E-cadherin: Roles of other cadherin superfamily members in cancer","volume":"14","year":"2014","journal-title":"Nat. Rev. Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1083\/jcb.201008173","article-title":"Evolution: Structural and functional diversity of cadherin at the adherens junction","volume":"193","author":"Oda","year":"2011","journal-title":"J. Cell Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"a003053","DOI":"10.1101\/cshperspect.a003053","article-title":"Structure and biochemistry of cadherins and catenins","volume":"1","author":"Shapiro","year":"2009","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/nrm3431","article-title":"A bigger picture: Classical cadherins and the dynamic actin cytoskeleton","volume":"13","author":"Ratheesh","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1152\/physrev.00035.2003","article-title":"Endothelial cell-to-cell junctions: Molecular organization and role in vascular homeostasis","volume":"84","author":"Bazzoni","year":"2004","journal-title":"Physiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lin, W.H., Cooper, L.M., and Anastasiadis, P.Z. (2023). Cadherins and catenins in cancer: Connecting cancer pathways and tumor microenvironment. Front. Cell Dev. Biol., 11.","DOI":"10.3389\/fcell.2023.1137013"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Yu, W., Yang, L., Li, T., and Zhang, Y. (2019). Cadherin Signaling in Cancer: Its Functions and Role as a Therapeutic Target. Front. Oncol., 9.","DOI":"10.3389\/fonc.2019.00989"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1006\/geno.2000.6305","article-title":"Characterization of three novel human cadherin genes (CDH7, CDH19, and CDH20) clustered on chromosome 18q22\u2013q23 and with high homology to chicken cadherin-7","volume":"68","author":"Kools","year":"2000","journal-title":"Genomics"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1007\/s12015-022-10460-7","article-title":"New Insights on the Role of Satellite Glial Cells","volume":"19","author":"Lu","year":"2023","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1053\/j.gastro.2021.10.001","article-title":"\u201cGoing the Extra Mile\u201d: A Sox10 Target, Cdh19, is Required for Sacral NC Migration in ENS Development","volume":"162","author":"Avila","year":"2022","journal-title":"Gastroenterology"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Hassan, S.Y., Flanagan, T.W., Hassan, S.L., Facca, S., Haikel, Y., and Hassan, M. (2025). Mucosal Melanoma: Mechanisms of Its Etiology, Progression, Resistance and Therapy. Cells, 14.","DOI":"10.3390\/cells14231884"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Atanasescu, V.P., Breazu, A., Oprea, S., Porosnicu, A.L., Oproiu, A., R\u0103doi, M.P., Munteanu, O., and Pantu, C. (2025). The Neuro-Melanoma Singularity: Convergent Evolution of Neural and Melanocytic Networks in Brain Metastatic Adaptation. Biomolecules, 15.","DOI":"10.3390\/biom15121683"},{"key":"ref_15","first-page":"10","article-title":"Cancer statistics, 2025","volume":"75","author":"Siegel","year":"2025","journal-title":"CA Cancer J. Clin."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1245\/s10434-018-6513-7","article-title":"Melanoma Staging: American Joint Committee on Cancer (AJCC) 8th Edition and Beyond","volume":"25","author":"Gershenwald","year":"2018","journal-title":"Ann. Surg. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1038\/s41571-023-00760-3","article-title":"Personalizing neoadjuvant immune-checkpoint inhibition in patients with melanoma","volume":"20","author":"Lucas","year":"2023","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1038\/nrc3239","article-title":"The blockade of immune checkpoints in cancer immunotherapy","volume":"12","author":"Pardoll","year":"2012","journal-title":"Nat. Rev. Cancer"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zhao, Z., Ding, Y., Tran, L.J., Chai, G., and Lin, L. (2023). Innovative breakthroughs facilitated by single-cell multi-omics: Manipulating natural killer cell functionality correlates with a novel subcategory of melanoma cells. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1196892"},{"key":"ref_20","first-page":"102401","article-title":"Development of novel anti-CDH1\/E-cadherin monoclonal antibodies for versatile applications","volume":"45","author":"Ubukata","year":"2026","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_21","first-page":"102138","article-title":"Development of an anti-CDH15\/M-cadherin monoclonal antibody Ca(15)Mab-1 for flow cytometry, immunoblotting, and immunohistochemistry","volume":"43","author":"Ubukata","year":"2025","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.pep.2014.01.009","article-title":"PA tag: A versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin","volume":"95","author":"Fujii","year":"2014","journal-title":"Protein Expr. Purif."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1089\/mab.2016.0039","article-title":"MAP Tag: A Novel Tagging System for Protein Purification and Detection","volume":"35","author":"Fujii","year":"2016","journal-title":"Monoclon. Antib. Immunodiagn. Immunother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"21679436261428408","DOI":"10.1177\/21679436261428408","article-title":"Development of Anti-Human Cadherin-26 Monoclonal Antibody, Ca26Mab-6, for Flow Cytometry","volume":"9","author":"Satofuka","year":"2026","journal-title":"Monoclon. Antib. Immunodiagn. Immunother."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3171\/2014.9.JNS132373","article-title":"Myelin-forming cell-specific cadherin-19 is a marker for minimally infiltrative glioblastoma stem-like cells","volume":"122","author":"Zorniak","year":"2015","journal-title":"J. Neurosurg."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Ubukata, R., Ohishi, T., Kaneko, M.K., Suzuki, H., and Kato, Y. (2025). EphB2-Targeting Monoclonal Antibodies Exerted Antitumor Activities in Triple-Negative Breast Cancer and Lung Mesothelioma Xenograft Models. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202507.2654.v1"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kaneko, M.K., Suzuki, H., Ohishi, T., Nakamura, T., Tanaka, T., and Kato, Y. (2024). A Cancer-Specific Monoclonal Antibody against HER2 Exerts Antitumor Activities in Human Breast Cancer Xenograft Models. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25031941"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1056\/NEJMoa1904059","article-title":"Five-Year Outcomes with Dabrafenib plus Trametinib in Metastatic Melanoma","volume":"381","author":"Robert","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1056\/NEJMoa1910836","article-title":"Five-Year Survival with Combined Nivolumab and Ipilimumab in Advanced Melanoma","volume":"381","author":"Larkin","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/S1470-2045(19)30388-2","article-title":"Pembrolizumab versus ipilimumab in advanced melanoma (KEYNOTE-006): Post-hoc 5-year results from an open-label, multicentre, randomised, controlled, phase 3 study","volume":"20","author":"Robert","year":"2019","journal-title":"Lancet Oncol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1056\/NEJMoa1709684","article-title":"Overall Survival with Combined Nivolumab and Ipilimumab in Advanced Melanoma","volume":"377","author":"Wolchok","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.cell.2023.11.037","article-title":"A TCF4-dependent gene regulatory network confers resistance to immunotherapy in melanoma","volume":"187","author":"Pozniak","year":"2024","journal-title":"Cell"}],"container-title":["Current Issues in Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1467-3045\/48\/3\/307\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T05:29:32Z","timestamp":1773466172000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1467-3045\/48\/3\/307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,12]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["cimb48030307"],"URL":"https:\/\/doi.org\/10.3390\/cimb48030307","relation":{},"ISSN":["1467-3045"],"issn-type":[{"value":"1467-3045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,12]]}}}