{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T22:46:33Z","timestamp":1784673993292,"version":"3.55.0"},"reference-count":139,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Hampea rovirosae Standl. is traditionally used by local communities to treat infections, pain-related conditions, and to reduce blood sugar levels. In this investigation, we produced aqueous, ethanolic, and hydroethanolic extracts of H. rovirosae and assessed their antioxidant, antibacterial, and antihyperglycemic properties in addition to their phytochemical profiles and contents. The phytochemical characterization was performed through a targeted chromatographic and mass spectrometric analysis of phenolic compounds and the quantitation of total phenolic content (TPC), total flavonoid content (TFC), and total tannin content (TTC) by spectrometric assays. The antioxidant capacity was assessed using the DPPH, ABTS, and FRAP assays, and the antibacterial activity was determined by disk diffusion (DD) and minimum inhibitory concentration (MIC) methods. In addition, antihyperglycemic activity was evaluated by inhibiting \u03b1-amylase and \u03b1-glucosidase. Phytochemical analysis was performed using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS), employing a targeted analysis approach based on comparing retention times and fragmentation patterns with standards and databases. This analysis revealed a phytochemical profile dominated by phenolic compounds, with quercetin-3-glucoside (155,930.2), caffeic acid (134,399.1), catechin (98,408.8), procyanidin B2 (85,661.7), protocatechuic acid (83,824.3), and epicatechin (53,704.1) being the major metabolites. The hydroethanolic extract exhibited the highest phenolic (426.70 mg GAE\/g), flavonoids (119.17 mg CE\/g), and tannin (324.46 mg GAE\/g) contents, as well as the strongest antioxidant capacity in the DPPH and FRAP assays. Regarding the antibacterial effects, the aqueous extract inhibited Salmonella typhimurium and Escherichia coli, while the hydroethanolic extract was active against S. aureus, B. cereus, and B. subtilis. In enzyme inhibition assays, the hydroethanolic extract showed strong \u03b1-glucosidase inhibition and moderate \u03b1-amylase inhibition. The findings provide preliminary scientific evidence of the antioxidant and biological activities of Hampea rovirosae in vitro, supporting its traditional use, which should be validated through vivo trials.<\/jats:p>","DOI":"10.3390\/cimb48030327","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T11:50:36Z","timestamp":1773921036000},"page":"327","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phytochemical Profile and Bioactive Potential of Hampea rovirosae\u00a0Standl.: Antioxidant, Antimicrobial, and Carbohydrate-Hydrolyzing Enzyme Inhibitory Activities"],"prefix":"10.3390","volume":"48","author":[{"given":"Maria Candelaria","family":"Tejero-Rivas","sequence":"first","affiliation":[{"name":"Academic Division of Agricultural Sciences, Ju\u00e1rez Autonomous University of Tabasco, Carretera, Villahermosa-Teapa Km 25, Villahermosa CP 86298, Mexico"},{"name":"National Technological Institute of Mexico, Villahermosa Campus, Villahermosa-Frontera Highway, Km. 3.5 Industrial City of Villahermosa, Villahermosa CP 86010, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4390-8337","authenticated-orcid":false,"given":"Jos\u00e9 Rodolfo","family":"Vel\u00e1zquez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Academic Division of Agricultural Sciences, Ju\u00e1rez Autonomous University of Tabasco, Carretera, Villahermosa-Teapa Km 25, Villahermosa CP 86298, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minerva Aurora","family":"Hern\u00e1ndez-Gallegos","sequence":"additional","affiliation":[{"name":"Multidisciplinary Academic Division of Jalpa de M\u00e9ndez, Ju\u00e1rez Autonomous University of Tabasco, State Highway Villahermosa-Comalcalco Km. 27+000 s\/n Rancher\u00eda Ribera Alta, Jalpa de M\u00e9ndez CP 86205, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4353-2870","authenticated-orcid":false,"given":"Angelica Alejandra","family":"Ochoa-Flores","sequence":"additional","affiliation":[{"name":"Academic Division of Agricultural Sciences, Ju\u00e1rez Autonomous University of Tabasco, Carretera, Villahermosa-Teapa Km 25, Villahermosa CP 86298, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1940-895X","authenticated-orcid":false,"given":"Rodolfo","family":"Osorio-Osorio","sequence":"additional","affiliation":[{"name":"Academic Division of Agricultural Sciences, Ju\u00e1rez Autonomous University of Tabasco, Carretera, Villahermosa-Teapa Km 25, Villahermosa CP 86298, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7006-9527","authenticated-orcid":false,"given":"Juan","family":"Guzm\u00e1n-Ceferino","sequence":"additional","affiliation":[{"name":"Academic Division of Agricultural Sciences, Ju\u00e1rez Autonomous University of Tabasco, Carretera, Villahermosa-Teapa Km 25, Villahermosa CP 86298, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Caba\u00f1as-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Unidad Professional Interdisciplinaria de Ingenier\u00eda Campus, Zacatecas-Instituto Polit\u00e9cnico Nacional, Calle Circuito del Gato No. 202, Col. 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