{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:02:35Z","timestamp":1785402155587,"version":"3.56.0"},"reference-count":27,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordination for the Improvement of Higher Education Personnel","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Council for Scientific and Technological Development CNPq","award":["303038\/2021-7"],"award-info":[{"award-number":["303038\/2021-7"]}]},{"award":["303038\/2021-7"],"award-info":[{"award-number":["303038\/2021-7"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Background\/Objectives: Acute myocardial infarction (AMI) remains a major cause of global morbidity and mortality and is a leading factor in the development of heart failure. This study investigated the regenerative potential of decellularized human amniotic membrane (HAM) and Wharton\u2019s jelly (WJ) in a rat model of left ventricular dysfunction induced by acute myocardial infarction (AMI). Methods: Twenty-three rats underwent left anterior descending coronary artery ligation and were randomized into three groups: control (saline), WJ (decellularized WJ), and HAM (decellularized HAM). Results: After 30 days, echocardiographic, histopathological, and immunohistochemical assessments were performed. No significant differences in ventricular function were observed among groups. However, the HAM-treated group showed a significant reduction in myocardial fibrosis compared with the control (p = 0.009), suggesting attenuation of post-infarction remodeling. Despite the absence of measurable functional recovery, HAM demonstrated potential to promote more favorable tissue organization. Study limitations include the lack of a sham-operated group, short follow-up period, and absence of quantitative decellularization validation. Conclusions: Overall, the results indicate that decellularized HAM may act as a structural modulator of myocardial remodeling, warranting further studies with longer follow-up and combination approaches, such as cell-based or growth factor-enhanced therapies.<\/jats:p>","DOI":"10.3390\/cimb48060579","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:37:26Z","timestamp":1780454246000},"page":"579","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Evaluation of Decellularized Human Amniotic Membrane and Wharton\u2019s Jelly in a Rat Model of Myocardial Infarction: Experimental Study"],"prefix":"10.3390","volume":"48","author":[{"given":"Marcos Ant\u00f4nio","family":"Denk","sequence":"first","affiliation":[{"name":"Graduate Program in Health Sciences, School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5885-7854","authenticated-orcid":false,"given":"Isabella Cristina Mendes","family":"Rossa","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luize Kremer","family":"Gamba","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Clara Faidiga","family":"Silva","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Let\u00edcia de","family":"Bortolo","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4908-2257","authenticated-orcid":false,"given":"Paulo Cesar Lock","family":"Silveira","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Physiopathology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Santa Catarina 88806-000, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila da","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Physiopathology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Santa Catarina 88806-000, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falio Cesar","family":"Francisco","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, Paran\u00e1, Brazil"},{"name":"Postgraduate Program in Biomedical Engineering (PPGEB-CT), Universidade Tecnol\u00f3gico Federal do Paran\u00e1, Curitiba 81310-900, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz C\u00e9sar","family":"Guarita-Souza","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2982","DOI":"10.1016\/j.jacc.2020.11.010","article-title":"GBD-NHLBI-JACC Global Burden of Cardiovascular Diseases Writing Group. 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