{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T13:03:59Z","timestamp":1785157439201,"version":"3.55.0"},"reference-count":45,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2025YFD2400300"],"award-info":[{"award-number":["2025YFD2400300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Guangxi Natural Science Foundation","doi-asserted-by":"publisher","award":["2026GXNSFBA00640361"],"award-info":[{"award-number":["2026GXNSFBA00640361"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Fujian Natural Science Foundation","doi-asserted-by":"publisher","award":["2023J01143"],"award-info":[{"award-number":["2023J01143"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fund of Engineering Research Center of the Modern Technology for Eel Industry","award":["RE202407"],"award-info":[{"award-number":["RE202407"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Curr. Issues Mol. Biol.","CIMB"],"abstract":"<jats:p>Pathogenic Vibrio species are a major cause of disease outbreaks in aquaculture, leading to substantial economic losses and posing risks to food safety. The increasing prevalence of antibiotic resistance among these pathogens has created an urgent need for alternative antimicrobial strategies. Phage-derived endolysins, which specifically degrade bacterial peptidoglycan, have emerged as promising candidates to replace or supplement conventional antibiotics. In this study, we systematically characterized the endolysin LysV039C from the Vibrio alginolyticus phage phiV039C. Whole-genome analysis identified a 462 bp endolysin gene, which was cloned into the pET-28a vector, expressed in E. coli BL21, and purified. The lytic activity of the recombinant enzyme was evaluated using a turbidity reduction assay. LysV039C showed the strongest activity (58.9%) against host bacteria in the logarithmic phase, with peak activity (60.7\u201364.1%) achieved at 53 \u03bcg\/mL, 35 \u00b0C, and neutral pH. The addition of 2.5 mM EDTA enhanced activity to 63.8%, whereas 10 mM divalent metal ions strongly inhibited the enzyme (&lt;6.5%). Lytic spectrum analysis demonstrated that LysV039C exhibited a broader lytic spectrum against six Vibrio species than its parent phage phiV039C. Overall, LysV039C combines high environmental adaptability (alkaline tolerance, suitability for aquaculture temperature ranges) with efficient and broadened lytic activity against multiple Vibrio species. These findings provide a foundation for developing eco-friendly agents for the prevention and control of pathogenic Vibrio and offer a valuable reference for the mining of other phage-derived antibacterials against aquatic pathogens.<\/jats:p>","DOI":"10.3390\/cimb48080754","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T11:54:26Z","timestamp":1785153266000},"page":"754","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recombinant Expression and Activity Analysis of a Vibrio alginolyticus Phage-Derived Endolysin LysV039C"],"prefix":"10.3390","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0315-2458","authenticated-orcid":false,"given":"Yiyuan","family":"Yu","sequence":"first","affiliation":[{"name":"Engineering Research Center of the Modern Technology for Eel Industry of Education Ministry, Fisheries College of Jimei University, Xiamen 361021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Ye","sequence":"additional","affiliation":[{"name":"Engineering Research Center of the Modern Technology for Eel Industry of Education Ministry, Fisheries College of Jimei University, Xiamen 361021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6960-3585","authenticated-orcid":false,"given":"Hongjiao","family":"Cai","sequence":"additional","affiliation":[{"name":"Engineering Research Center of the Modern Technology for Eel Industry of Education Ministry, Fisheries College of Jimei University, Xiamen 361021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongqin","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center of the Modern Technology for Eel Industry of Education Ministry, Fisheries College of Jimei University, Xiamen 361021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Engineering Research Center of the Modern Technology for Eel Industry of Education Ministry, Fisheries College of Jimei University, Xiamen 361021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guixiang","family":"Tong","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5380-178X","authenticated-orcid":false,"given":"Mingfeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Modern Biology and Medicine Industry College, Hangzhou Normal University (Cangqian Campus), Hangzhou 311231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibiao","family":"Weng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Eel Aquaculture and Processing of Fujian Province, Fuzhou 350200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglian","family":"Tan","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2469-3724","authenticated-orcid":false,"given":"Mao","family":"Lin","sequence":"additional","affiliation":[{"name":"Engineering Research Center of the Modern Technology for Eel Industry of Education Ministry, Fisheries College of Jimei University, Xiamen 361021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kalatzis, P.G., Bast\u00edas, R., Kokkari, C., and Katharios, P. 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