{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:25:02Z","timestamp":1786116302067,"version":"build-2736575974"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central Guided Local Science and Technology Development Fund","award":["2024ZYD0284"],"award-info":[{"award-number":["2024ZYD0284"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Curr. Issues Mol. Biol.","CIMB"],"abstract":"<jats:p>Akebia trifoliata is an important Chinese traditional medicinal plant, and its abundant triterpenoids are the primary active medicinal components. However, the oxidosqualene cyclase (OSC) gene family responsible for constructing their triterpenoid skeletons has not yet been systematically characterized. In this study, nine AktOSC genes were identified genome-wide in A. trifoliata. Phylogenetic analysis revealed that most AktOSC members formed independent evolutionary subclades. Synteny and selective pressure analyses indicated that multiple duplication modes collectively contributed to the expansion of the AktOSC family, and all members were subjected to strong purifying selection. Sequence alignment showed that all members possess the characteristic motifs of the OSC family, whereas amino acid substitutions at critical active-site residues hinted at potential catalytic product diversity. Cis-acting element prediction revealed an abundance of environmental and phytohormone responsiveness elements within the AktOSC promoter regions. Expression profiling revealed that AktOSC genes exhibited distinct expression patterns across tissues, developmental stages, and disease-susceptibility groups. Finally, AktOSC8, which was specifically and highly expressed in the pericarp, was identified as a highest-priority candidate gene. Collectively, these findings provide essential foundational insights for further elucidating the mechanisms underlying triterpenoid biosynthesis and accumulation in A. trifoliata.<\/jats:p>","DOI":"10.3390\/cimb48080795","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T08:08:02Z","timestamp":1786003682000},"page":"795","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Identification and Expression Profiling of the Oxidosqualene Cyclase Gene Family in Akebia trifoliata Across Fruit Development and Disease-Susceptibility Groups"],"prefix":"10.3390","volume":"48","author":[{"given":"Hefei","family":"Rao","sequence":"first","affiliation":[{"name":"Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1630-5662","authenticated-orcid":false,"given":"Hao","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Yi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfeng","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiquan","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4603-6960","authenticated-orcid":false,"given":"Peigao","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102701","DOI":"10.1016\/j.pbi.2025.102701","article-title":"Biosynthesis of Triterpenoids in Plants: Pathways, Regulation, and Biological Functions","volume":"85","author":"Dong","year":"2025","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Du, Y., Fu, X., Chu, Y., Wu, P., Liu, Y., Ma, L., Tian, H., and Zhu, B. (2022). Biosynthesis and the Roles of Plant Sterols in Development and Stress Responses. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23042332"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0163-7827(02)00047-4","article-title":"The Role of Sterols in Plant Growth and Development","volume":"42","author":"Schaller","year":"2003","journal-title":"Prog. Lipid Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1146\/annurev-arplant-050312-120229","article-title":"Triterpene Biosynthesis in Plants","volume":"65","author":"Thimmappa","year":"2014","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.3390\/molecules14062016","article-title":"Pentacyclic Triterpene Distribution in Various Plants\u2014Rich Sources for a New Group of Multi-Potent Plant Extracts","volume":"14","author":"Trojan","year":"2009","journal-title":"Molecules"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nrc2129","article-title":"Triterpenoids and Rexinoids as Multifunctional Agents for the Prevention and Treatment of Cancer","volume":"7","author":"Liby","year":"2007","journal-title":"Nat. Rev. Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jep.2005.05.024","article-title":"Oleanolic Acid and Ursolic Acid: Research Perspectives","volume":"100","author":"Liu","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"13431","DOI":"10.1073\/pnas.231324698","article-title":"A New Class of Oxidosqualene Cyclases Directs Synthesis of Antimicrobial Phytoprotectants in Monocots","volume":"98","author":"Haralampidis","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.phytochem.2003.11.014","article-title":"On the Origins of Triterpenoid Skeletal Diversity","volume":"65","author":"Xu","year":"2004","journal-title":"Phytochemistry"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6816","DOI":"10.1021\/ja0010709","article-title":"Mutational Studies on Triterpene Synthases: Engineering Lupeol Synthase into \u03b2-Amyrin Synthase","volume":"122","author":"Kushiro","year":"2000","journal-title":"J. Am. Chem. Soc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1039\/b616857b","article-title":"Enzymatic Synthesis of Cyclic Triterpenes","volume":"24","author":"Abe","year":"2007","journal-title":"Nat. Prod. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0968-0004(94)90276-3","article-title":"A Specific Amino Acid Repeat in Squalene and Oxidosqualene Cyclases","volume":"19","author":"Poralla","year":"1994","journal-title":"Trends Biochem. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"E4407","DOI":"10.1073\/pnas.1605509113","article-title":"A Conserved Amino Acid Residue Critical for Product and Substrate Specificity in Plant Triterpene Synthases","volume":"113","author":"Salmon","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"eadh1418","DOI":"10.1126\/sciadv.adh1418","article-title":"Discovering a Uniform Functional Trade-off of the CBC-Type 2,3-Oxidosqualene Cyclases and Deciphering Its Chemical Logic","volume":"9","author":"Zhang","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.16943\/ptinsa\/2016\/48578","article-title":"Biosynthesis of Structurally Diverse Triterpenes in Plants: The Role of Oxidosqualene Cyclases","volume":"82","author":"Ghosh","year":"2016","journal-title":"Proc. Indian Natl. Sci. Acad."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1111\/j.1469-8137.2011.03997.x","article-title":"Divergent Evolution of Oxidosqualene Cyclases in Plants","volume":"193","author":"Xue","year":"2012","journal-title":"New Phytol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4","DOI":"10.21273\/HORTSCI.45.1.4","article-title":"Akebia: A Potential New Fruit Crop in China","volume":"45","author":"Li","year":"2010","journal-title":"HortScience"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"114245","DOI":"10.1016\/j.jep.2021.114245","article-title":"Traditional Uses, Phytochemistry, Pharmacology, and Toxicology of Akebiae Caulis and Its Synonyms: A Review","volume":"277","author":"Li","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"114486","DOI":"10.1016\/j.jep.2021.114486","article-title":"Akebia quinata and Akebia trifoliata\u2014A Review of Phytochemical Composition, Ethnopharmacological Approaches and Biological Studies","volume":"280","author":"Dobrowolska","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"104549","DOI":"10.1016\/j.arabjc.2023.104549","article-title":"Comparative Chemical Diversity and Antioxidant Activities of Three Species of Akebia Herbal Medicines","volume":"16","author":"Liu","year":"2023","journal-title":"Arab. J. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1248\/cpb.51.960","article-title":"Triterpenes and Triterpene Saponins from the Stems of Akebia trifoliata","volume":"51","author":"Mimaki","year":"2003","journal-title":"Chem. Pharm. Bull."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1016\/j.phytochem.2006.09.003","article-title":"Triterpenoid Saponins and Phenylethanoid Glycosides from Stem of Akebia trifoliata var. australis","volume":"67","author":"Gao","year":"2006","journal-title":"Phytochemistry"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.3390\/molecules19044301","article-title":"Bioactive 30-Noroleanane Triterpenes from the Pericarps of Akebia trifoliata","volume":"19","author":"Wang","year":"2014","journal-title":"Molecules"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1093\/jaoac\/89.4.942","article-title":"Determination of Aristolochic Acid in Botanicals and Dietary Supplements by Liquid Chromatography with Ultraviolet Detection and by Liquid Chromatography\/Mass Spectrometry: Single Laboratory Validation Confirmation","volume":"89","author":"Trujillo","year":"2006","journal-title":"J. AOAC Int."},{"key":"ref_25","first-page":"925","article-title":"Determination of Aristolochic Acid I in Botanicals and Dietary Supplements Potentially Contaminated with Aristolochic Acid I Using LC-UV with Confirmation by LC\/MS: Collaborative Study","volume":"90","author":"Sorenson","year":"2007","journal-title":"J. AOAC Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1007\/s10157-003-0229-z","article-title":"The Nephrotoxicity of Aristolochia manshuriensis in Rats Is Attributable to Its Aristolochic Acids","volume":"7","author":"Liu","year":"2003","journal-title":"J. Clin. Exp. Nephrol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1111\/tpj.16011","article-title":"The Chromosome-Level Genome of Akebia trifoliata as an Important Resource to Study Plant Evolution and Environmental Adaptation in the Cretaceous","volume":"112","author":"Zhong","year":"2022","journal-title":"Plant J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1093\/jxb\/erae189","article-title":"Improving Transformation and Regeneration Efficiency in Medicinal Plants: Insights from Other Recalcitrant Species","volume":"76","author":"Bennur","year":"2025","journal-title":"J. Exp. Bot."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1111\/jipb.70110","article-title":"Genome Editing of Medicinal Plants: Advances, Challenges, and Prospects","volume":"68","author":"Chen","year":"2026","journal-title":"J. Integr. Plant Biol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Zhong, S., Yang, H., Guan, J., Shen, J., Ren, T., Li, Z., Tan, F., Li, Q., and Luo, P. (2022). Characterization of the MADS-Box Gene Family in Akebia trifoliata and Their Evolutionary Events in Angiosperms. Genes, 13.","DOI":"10.3390\/genes13101777"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1111\/j.1469-8137.2011.03712.x","article-title":"Investigation of the Potential for Triterpene Synthesis in Rice through Genome Mining and Metabolic Engineering","volume":"191","author":"Inagaki","year":"2011","journal-title":"New Phytol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Yi, X., Chen, W., Guan, J., Zhu, J., Zhang, Q., Yang, H., Yang, H., Zhong, S., Chen, C., and Tan, F. (2023). Genome-Wide Analysis of the Polygalacturonase Gene Family Sheds Light on the Characteristics, Evolutionary History, and Putative Function of Akebia trifoliata. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242316973"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Eddy, S.R. (2011). Accelerated Profile HMM Searches. PLoS Comput. Biol., 7.","DOI":"10.1371\/journal.pcbi.1002195"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and Applications. BMC Bioinf., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"D499","DOI":"10.1093\/nar\/gkaf1023","article-title":"SMART V10: Three Decades of the Protein Domain Annotation Resource","volume":"54","author":"Letunic","year":"2026","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3784","DOI":"10.1093\/nar\/gkg563","article-title":"ExPASy: The Proteomics Server for in-Depth Protein Knowledge and Analysis","volume":"31","author":"Gasteiger","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"W215","DOI":"10.1093\/nar\/gkae237","article-title":"DeepLoc 2.1: Multi-Label Membrane Protein Type Prediction Using Protein Language Models","volume":"52","author":"Teufel","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"D265","DOI":"10.1093\/nar\/gkz991","article-title":"CDD\/SPARCLE: The Conserved Domain Database in 2020","volume":"48","author":"Lu","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"W39","DOI":"10.1093\/nar\/gkv416","article-title":"The MEME Suite","volume":"43","author":"Bailey","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1016\/j.molp.2023.09.010","article-title":"TBtools-II: A \u201cOne for All, All for One\u201d Bioinformatics Platform for Biological Big-Data Mining","volume":"16","author":"Chen","year":"2023","journal-title":"Mol. Plant"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1093\/bioinformatics\/btp348","article-title":"trimAl: A Tool for Automated Alignment Trimming in Large-Scale Phylogenetic Analyses","volume":"25","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: Recent Updates to the Phylogenetic Tree Display and Annotation Tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e49","DOI":"10.1093\/nar\/gkr1293","article-title":"MCScanX: A Toolkit for Detection and Evolutionary Analysis of Gene Synteny and Collinearity","volume":"40","author":"Wang","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a Database of Plant Cis-Acting Regulatory Elements and a Portal to Tools for in Silico Analysis of Promoter Sequences","volume":"30","author":"Lescot","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","article-title":"Graph-Based Genome Alignment and Genotyping with HISAT2 and HISAT-Genotype","volume":"37","author":"Kim","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie Enables Improved Reconstruction of a Transcriptome from RNA-Seq Reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/tpj.14132","article-title":"Multi-Omics Data-Driven Investigations of Metabolic Diversity of Plant Triterpenoids","volume":"97","author":"Shang","year":"2019","journal-title":"Plant J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/10409238.2021.1979458","article-title":"Diverse Triterpene Skeletons Are Derived from the Expansion and Divergent Evolution of 2,3-Oxidosqualene Cyclases in Plants","volume":"57","author":"Wang","year":"2022","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/S0021-9258(17)42179-X","article-title":"Active Site Mapping of Affinity-Labeled Rat Oxidosqualene Cyclase","volume":"269","author":"Abe","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1021\/ja963227w","article-title":"Methodology for the Preparation of Pure Recombinant S. cerevisiae Lanosterol Synthase Using a Baculovirus Expression System. Evidence That Oxirane Cleavage and a-Ring Formation Are Concerted in the Biosynthesis of Lanosterol from 2,3-Oxidosqualene","volume":"119","author":"Corey","year":"1997","journal-title":"J. Am. Chem. Soc."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1021\/ja963228o","article-title":"Studies on the Substrate Binding Segments and Catalytic Action of Lanosterol Synthase. Affinity Labeling with Carbocations Derived from Mechanism-Based Analogs of 2,3-Oxidosqualene and Site-Directed Mutagenesis Probes","volume":"119","author":"Corey","year":"1997","journal-title":"J. Am. Chem. Soc."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4507","DOI":"10.1021\/acs.biochem.0c00762","article-title":"Crystal Structure and Mechanistic Molecular Modeling Studies of Mycobacterium Tuberculosis Diterpene Cyclase Rv3377c","volume":"59","author":"Zhang","year":"2020","journal-title":"Biochemistry"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1126\/science.277.5333.1811","article-title":"Structure and Function of a Squalene Cyclase","volume":"277","author":"Wendt","year":"1997","journal-title":"Science"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3933","DOI":"10.1021\/acs.jcim.4c00297","article-title":"Adjusting Catalytic Activity of \u03b2-Amyrin Synthase GgBAS by Utilizing the Plasticity Residues of an Active Site","volume":"64","author":"Zheng","year":"2024","journal-title":"J. Chem. Inf. Model."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Guo, C., Xu, S., and Guo, X. (2025). Genome-Wide Analysis of Oxidosqualene Cyclase Genes in Artemisia Annua: Evolution, Expression, and Potential Roles in Triterpenoid Biosynthesis. Curr. Issues Mol. Biol., 47.","DOI":"10.20944\/preprints202506.0611.v1"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1093\/plphys\/kiad643","article-title":"OXIDOSQUALENE CYCLASE 1 and 2 Influence Triterpene Biosynthesis and Defense in Nicotiana attenuata","volume":"194","author":"Yang","year":"2024","journal-title":"Plant Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1007\/s11105-024-01496-9","article-title":"Transcriptomic Analyses of Akebiae Fructus and Identification of Genes Related to Triterpenoid Saponin Biosynthesis","volume":"43","author":"Fang","year":"2024","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s00425-023-04268-z","article-title":"Contemporary Understanding of Transcription Factor Regulation of Terpenoid Biosynthesis in Plants","volume":"259","author":"Huang","year":"2023","journal-title":"Planta"}],"container-title":["Current Issues in Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1467-3045\/48\/8\/795\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T09:06:23Z","timestamp":1786007183000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1467-3045\/48\/8\/795"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,6]]},"references-count":60,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["cimb48080795"],"URL":"https:\/\/doi.org\/10.3390\/cimb48080795","relation":{},"ISSN":["1467-3045"],"issn-type":[{"value":"1467-3045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,6]]}}}