{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T18:58:58Z","timestamp":1787425138559,"version":"build-2736575974"},"reference-count":64,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinics and Practice"],"abstract":"<jats:p>Background: The aging process starts in the center of the face, in the periocular region and around the mouth, with a combination of volume loss, tissue descent, deepened wrinkles, and the loss of skin structure and quality. Recently, several studies have demonstrated the efficacy of therapies based on autologous adipose tissue grafting, which leverages the properties of stromal vascular fraction (SVF) and adipose-derived mesenchymal stem cells (ADSCs) to accelerate the regenerative processes of the skin. This study aims to verify the ability of guided superficial enhanced fluid fat injection (SEFFI) in the facial area to correct volume loss and skin aging, proving that this standardized procedure has a very low rate of complications. Methods: We retrospectively collected data from 2365 procedures performed in Italian centers between 2019 and 2021. Guided SEFFI was performed alone or combined with cosmetic treatments, including the use of hyaluronic acid filler, suspension threads, synthetic calcium hydroxylapatite, botulin toxin, and microneedling. Results: guided SEFFI was used alone in more than 60% of the patients and in all facial areas. In about one-tenth of the patients, guided SEFFI was combined with a botulin toxin treatment or hyaluronic acid filling. Other procedures were used more rarely. Ecchymosis in the donor or injection sites was the most frequent adverse event but was only observed in 14.2% and 38.6% of the patients, respectively. Conclusions: The guided SEFFI technique is standardized and minimally invasive, leading to very few complications. It constitutes a promising antiaging medical treatment that combines effectiveness, safety, and simplicity.<\/jats:p>","DOI":"10.3390\/clinpract13040085","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T12:45:53Z","timestamp":1691498753000},"page":"924-943","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Guided Superficial Enhanced Fluid Fat Injection (SEFFI) Procedures for Facial Rejuvenation: An Italian Multicenter Retrospective Case Report"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4105-725X","authenticated-orcid":false,"given":"Alessandro","family":"Gennai","sequence":"first","affiliation":[{"name":"Studio Gennai, 40122 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Baldessin","sequence":"additional","affiliation":[{"name":"Studio MB, 31100 Treviso, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1009-0527","authenticated-orcid":false,"given":"Fabrizio","family":"Melfa","sequence":"additional","affiliation":[{"name":"Mediaging Clinic Center, 90143 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Bovani","sequence":"additional","affiliation":[{"name":"Centro di Chirurgia Ambulatoriale Esculapio, 06124 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Camporese","sequence":"additional","affiliation":[{"name":"Studio Camporese, 35010 Cadoneghe, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Claysset","sequence":"additional","affiliation":[{"name":"BC Medical, 40137 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Colli","sequence":"additional","affiliation":[{"name":"Centro Podgora 7, 20122 Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Diaspro","sequence":"additional","affiliation":[{"name":"Rigeneralab Centre for Regenerative Medicine Torino, 10134 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalba","family":"Russo","sequence":"additional","affiliation":[{"name":"Estemed, 41122 Modena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Placido","family":"Strano","sequence":"additional","affiliation":[{"name":"Beaut\u00e8 Medical, 33170 Pordenone, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Bollero","sequence":"additional","affiliation":[{"name":"CTO Hospital, 10126 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Cappar\u00e8","sequence":"additional","affiliation":[{"name":"Studio Medico Strozzieri, 64021 Giulianova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Casadei","sequence":"additional","affiliation":[{"name":"Ambulatorio Casadei, 30174 Venezia-Mestre, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Gallo","sequence":"additional","affiliation":[{"name":"Centro Mediestelaser, 92024 Canicatt\u00ec, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1983-0592","authenticated-orcid":false,"given":"Domenico","family":"Piccolo","sequence":"additional","affiliation":[{"name":"Skin Center, 65121 Pescara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Salti","sequence":"additional","affiliation":[{"name":"Medlight Institute, 50144 Firenze, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piero","family":"Tesauro","sequence":"additional","affiliation":[{"name":"Poliambulatorio Gioia, 20124 Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1001\/archderm.138.11.1462","article-title":"Mechanisms of photoaging and chronological skin aging","volume":"138","author":"Fisher","year":"2002","journal-title":"Arch. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.fsc.2018.06.004","article-title":"Stem cells in dermatology and anti-aging care of the skin","volume":"26","author":"Taub","year":"2018","journal-title":"Facial Plast. Surg. Clin. N. Am."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jtv.2016.03.002","article-title":"Introduction to skin aging","volume":"26","author":"Tobin","year":"2017","journal-title":"J. Tissue Viability"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"64635","DOI":"10.1155\/2012\/646354","article-title":"Oxidative stress, mitochondrial dysfunction, and aging","volume":"2012","author":"Cui","year":"2012","journal-title":"J. Signal Transduct."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1097\/01.prs.0000265403.66886.54","article-title":"The fat compartments of the face: Anatomy and clinical implications for cosmetic surgery","volume":"119","author":"Rohrich","year":"2007","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S25","DOI":"10.1177\/1090820X10373360","article-title":"Facial aesthetic analysis","volume":"30","author":"Fitzgerald","year":"2010","journal-title":"Aesthetic Surg. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1097\/01.prs.0000299635.55815.2e","article-title":"The fat compartments of the face: Anatomy and clinical implications for cosmetic surgery","volume":"121","author":"McCafferty","year":"2008","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1046\/j.1524-4725.2000.00270.x","article-title":"Fat distribution: A morphologic study of the aging face","volume":"26","author":"Donofrio","year":"2000","journal-title":"Dermatol. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gaur, M., Dobke, M., and Lunyak, V.V. (2017). Mesenchymal stem cells from adipose tissue in clinical applications for dermatological indications and skin aging. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18010208"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.maturitas.2011.04.011","article-title":"Molecular aspects of skin ageing","volume":"69","author":"Nailor","year":"2011","journal-title":"Maturitas"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/1523-1747.ep12481882","article-title":"Oxytalan, elaulin and elastic fibers in the human skin","volume":"66","author":"Rodrigo","year":"1976","journal-title":"J. Investig. Dermatol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Gennai, A., Tesauro, P., Colli, M., Roda, B., and Zattoni, A. (2021). Infranatant portion of microfragmented adipose tissue: A promising source of SVF for the management of androgenic alopecia. J. Regen. Med., 1\u20137.","DOI":"10.31487\/j.RGM.2021.01.01"},{"key":"ref_13","first-page":"236","article-title":"Immunomodulatory Effect of Adipose-Derived Stem Cells: The Cutting Edge of Clinical Application Frontiers in Cell and Developmental","volume":"8","author":"Ceccarelli","year":"2020","journal-title":"Biology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s12265-011-9257-3","article-title":"Adult human adipose tissue contains several types of multipotent cells","volume":"4","author":"Tallone","year":"2011","journal-title":"J. Cardiovasc. Transl. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.3727\/096368914X685636","article-title":"Regional implantation of autologous adipose tissue-derived cells induces a prompt healing of long-lasting indolent digital ulcers in patients with systemic sclerosis","volume":"24","author":"Sambataro","year":"2015","journal-title":"Cell Transplant."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1097\/01.prs.0000256047.47909.71","article-title":"Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: A healing process mediated by adipose-derived adult stem cells","volume":"119","author":"Rigotti","year":"2007","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1093\/asj\/sjy142","article-title":"Characterization of the Tissue and Stromal Cell Components of Micro-Superficial Enhanced Fluid Fat Injection (Micro-SEFFI) for Facial Aging Treatment","volume":"40","author":"Rossi","year":"2018","journal-title":"Aesthetic Surg. J."},{"key":"ref_18","first-page":"7","article-title":"Human Adipose Tissue Possesses a Unique Population of Pluripotent Stem Cells with Nontumorigenic and Low Telomerase Activities: Potential Implications in Regenerative Medicine","volume":"2","author":"Ogura","year":"2013","journal-title":"Stem Cell Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1161\/01.CIR.0000114522.38265.61","article-title":"Plasticity of human adipose lineage cells toward endothelial cells: Physiological and therapeutic perspectives","volume":"109","author":"Silvestre","year":"2004","journal-title":"Circulation"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1177\/1090820X14528000","article-title":"Harvest of superficial layers of fat with a microcannula and isolation of adipose tissue-derived stromal and vascular cells","volume":"34","author":"Trivisonno","year":"2014","journal-title":"Aesthetic Surg. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1177\/1090820X12445082","article-title":"Sharp-needle intradermal fat grafting (SNIF)","volume":"32","author":"Zeltzer","year":"2012","journal-title":"Aesthetic Surg. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"88","DOI":"10.3389\/fcell.2019.00088","article-title":"Mechanical and Enzymatic Procedures to Isolate the Stromal Vascular Fraction from Adipose Tissue: Preliminary Results","volume":"7","author":"Senesi","year":"2019","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1089\/ten.tea.2008.0359","article-title":"Adipose stromal cells stimulate angiogenesis via promoting progenitor cell differentiation, secretion of angiogenic factors, and enhancing vessel maturation","volume":"15","author":"Rubina","year":"2009","journal-title":"Tissue Eng. Part A"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1634\/stemcells.2008-0273","article-title":"IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function","volume":"27","author":"Cai","year":"2009","journal-title":"Stem Cells"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.bbrc.2005.04.135","article-title":"Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo","volume":"332","author":"Cao","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1186\/s13287-017-0683-2","article-title":"Meta-analysis on the treatment of diabetic foot ulcers with autologous stem cells","volume":"8","author":"Guo","year":"2017","journal-title":"Stem Cell Res. Ther."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"532704","DOI":"10.4061\/2010\/532704","article-title":"Noncultured Autologous Adipose-Derived Stem Cells Therapy for Chronic Radiation Injury","volume":"2010","author":"Akita","year":"2010","journal-title":"Stem Cells Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1093\/asj\/sjv001","article-title":"Superficial Enhanced Fluid Fat Injection (SEFFI) to Correct Volume Defects and Skin Aging of the Face and Periocular Region","volume":"35","author":"Bernardini","year":"2015","journal-title":"Aesthetic Surg. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1001\/jamafacial.2015.1146","article-title":"Superficial Enhanced Fluid Fat Injection for Volume Restoration and Skin Regeneration of the Periocular Aesthetic Unit. An Improved Fat Grafting Technique to enhance the beauty of the eye","volume":"18","author":"Bernardini","year":"2016","journal-title":"JAMA Plast. Facial Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"891","DOI":"10.2147\/CCID.S404248","article-title":"The Effectiveness of Injectable Hyaluronic Acid in the Improvement of the Facial Skin Quality: A Systematic Review","volume":"16","author":"Ghatge","year":"2023","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1093\/asj\/sjw084","article-title":"Skin Rejuvenation and Volume Enhancement with the Micro Superficial Enhanced Fluid Fat Injection (M-SEFFI) for Skin Aging of the Periocular and Perioral Regions","volume":"37","author":"Gennai","year":"2017","journal-title":"Aesthetic Surg. J."},{"key":"ref_32","first-page":"87","article-title":"Rejuvenation faciale par lifting endoscopique avec le petite incision incision vertical associ\u00e9e \u00e0 une injection superficielle de graisse: Un repositionement endoscopique, une regeneration tissulaire et une restauration volumetrique (La technique R3)","volume":"174","author":"Gennai","year":"2017","journal-title":"J. Med. Esth. Chir. Derm."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"23","DOI":"10.20517\/2347-9264.2018.28","article-title":"Rejuvenation of the centre of the face: A new paradigm Endoscopic lifting with fat grafting","volume":"5","author":"Pignata","year":"2018","journal-title":"Plast. Aesthet. Res."},{"key":"ref_34","first-page":"2","article-title":"Comparison of Harvesting and Processing Technique for Adipose Tissue Graft: Evaluation of Cell Viability","volume":"4","author":"Gennai","year":"2021","journal-title":"Int. J. Regen. Med."},{"key":"ref_35","first-page":"101","article-title":"Evaluation of the Viability and Phenotype of Adipose Derived Cells Harvested Using Different Harvesting and Processing Procedures: A Pilot Study","volume":"2","author":"Gennai","year":"2022","journal-title":"J. Stem Cells Clin. Pract."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"32","DOI":"10.12970\/2310-998X.2020.08.05","article-title":"SEFFI (Superficial Enhanced Fluid Fat Injection) for aesthetic and clinical regenerative treatments","volume":"8","author":"Gennai","year":"2020","journal-title":"Glob. J. Dermatol. Venereol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1093\/asj\/sjw106","article-title":"The Effects of Fat Harvesting and Preparation, Air Exposure, Obesity, and Stem Cell Enrichment on Adipocyte Viability Prior to Graft Transplantation","volume":"36","author":"Cucchiani","year":"2016","journal-title":"Aesthetic Surg. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1097\/PRS.0000000000001123","article-title":"Antiaging treatment of the facial skin by fat graft and adipose-derived stem cells","volume":"135","author":"Takiya","year":"2015","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_39","first-page":"2","article-title":"Evaluation of the Number, Biophysical and Multipotent Characteristics of Adipose Derived Stem Cells Harvested by SEFFI Procedure and Interaction with Different Type of Hyaluronic Acids","volume":"4","author":"Gennai","year":"2021","journal-title":"Int. J. Regen. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"17","DOI":"10.20517\/2347-9264.2018.10","article-title":"Key rheological properties of hyaluronic acid fillers: From tissue integration to product degradation","volume":"5","author":"Molliard","year":"2018","journal-title":"Plast. Aesthet. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1097\/PRS.0b013e3181c2a59a","article-title":"Human tissue-resident stem cells combined with hyaluronic acid gel provide fibrovascular-integrated soft-tissue augmentation in a murine photoaged skin model","volume":"125","author":"Altman","year":"2010","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.46889\/JRMBR.2022.3102","article-title":"Non-surgical Jowl and Jawline Rejuvenation with Resorbable Suspension Threads and Superficial Enhanced Fluid Fat Injection (SEFFI)","volume":"3","author":"Colli","year":"2022","journal-title":"J. Reg. Med. Biol. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e12714","DOI":"10.1111\/dth.12714","article-title":"Is skin microneedling a good alternative method of various skin defects removal","volume":"31","author":"Rotsztejn","year":"2018","journal-title":"Dermatol. Ther."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Gentile, P., and Garcovich, S. (2021). Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) against Ultraviolet (UV) Radiation Effects and the Skin Photoaging. Biomedicines, 9.","DOI":"10.3390\/biomedicines9050532"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1159\/000488992","article-title":"Basic Fibroblast Growth Factor Influences Epidermal Homeostasis of Living Skin Equivalents through Affecting Fibroblast Phenotypes and Functions","volume":"31","author":"Yang","year":"2018","journal-title":"Skin Pharmacol. Physiol."},{"key":"ref_46","unstructured":"Bolognia, J.L., Jorizzo, J.L., and Rapini, R.P. (2008). Dermatology, Mosby Elsevier. [2nd ed.]."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.cps.2016.03.003","article-title":"Overview of botulinum toxins for aesthetic uses","volume":"43","author":"Gart","year":"2016","journal-title":"Clin. Plast. Surg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"223","DOI":"10.2147\/CCID.S202919","article-title":"Botulinum toxin (Botox) A for reducing the appearance of facial wrinkles: A literature review of clinical use and pharmacological aspect","volume":"12","author":"Satriyasa","year":"2019","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.clindermatol.2021.08.020","article-title":"Carboxytherapy in dermatology","volume":"40","author":"Kroumpouzos","year":"2022","journal-title":"Clin. Dermatol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1111\/jocd.13470","article-title":"The use of Carboxytherapy alone or in combination with fractional CO2 laser for facial rejuvenation: A split-face comparative study","volume":"19","author":"Medhat","year":"2020","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1111\/jocd.12215","article-title":"Evaluation of lifting and antiwrinkle effects of calcium hydroxylapatite filler. In vitro quantification of contractile forces of human wrinkle and normal aged fibroblasts treated with calcium hydroxylapatite","volume":"15","author":"Robin","year":"2016","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"S8","DOI":"10.1093\/asj\/sjy011","article-title":"Physiochemical Characteristics of Calcium Hydroxylapatite (CaHA)","volume":"38","author":"Lorenc","year":"2018","journal-title":"Aesthetic Surg. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e112","DOI":"10.1097\/DSS.0000000000002375","article-title":"Hyperdiluted Calcium Hydroxylapatite 1:2 for Mid and Lower Facial Skin Rejuvenation: Efficacy and Safety","volume":"46","author":"Rovatti","year":"2020","journal-title":"Dermatol. Surg."},{"key":"ref_54","unstructured":"Bartoletti, E., Melfa, F., Renzi, M., and Rovatti, P. (2022). Systematic review of the literature on the properties, quality and reliability of calcium hydroxyapatite: Results of an Italian experts\u2019 meeting. Aesthetic Med., 8."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0094-1298(20)32343-9","article-title":"Structural fat grafts: The ideal filler?","volume":"28","author":"Coleman","year":"2001","journal-title":"Clin. Plast. Surg."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1097\/PRS.0b013e31829fe1b0","article-title":"Nanofat grafting basic research and clinical applications","volume":"132","author":"Tonnard","year":"2013","journal-title":"Plast. Reconstr. Surg. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1097\/IOP.0b013e318291662a","article-title":"Minimal incisions vertical endoscopic lifting and fat grafting as a systematic approach to the rejuvenation of the periocular esthetic unit","volume":"29","author":"Bernardini","year":"2013","journal-title":"Ophthalmic Plast. Reconstr. Surg."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1016\/j.bjps.2012.06.014","article-title":"Development of micro-injection as an innovative autologous fat graft technique: The use of adipose tissue as dermal filler","volume":"65","author":"Nguyen","year":"2012","journal-title":"J. Plast. Reconstr. Aesthet. Surg."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.cps.2016.03.017","article-title":"Fat, stem cells, and platelet-rich plasma","volume":"43","author":"James","year":"2016","journal-title":"Clin. Plast. Surg."},{"key":"ref_60","first-page":"e21709","article-title":"Adipose Adipose-derived stem cells regulate metabolic homeostasis and delay aging by promoting mitophagy","volume":"35","author":"Mengzhu","year":"2021","journal-title":"J. FASEB"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1097\/PRS.0000000000005625","article-title":"Synergistic Effect of Adipose-Derived Stem Cells and Fat Graft on Wrinkles in Aged Mice","volume":"143","author":"Kikap","year":"2019","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.jcyt.2015.04.004","article-title":"Qualitative and quantitative differences of adipose-derived stromal cells from superficial and deep subcutaneous lipoaspirates: A matter of fat","volume":"17","author":"Cicione","year":"2015","journal-title":"Cytotherapy"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Schiraldi, L., Sapino, G., Meuli, J., Maruccia, M., Cherubino, M., Raffoul, W., and di Summa, P.G. (2022). Facial Fat Grafting (FFG): Worth the Risk? A Systematic Review of Complications and Critical Appraisal. J. Clin. Med., 11.","DOI":"10.3390\/jcm11164708"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.cxom.2017.10.002","article-title":"Autologous Fat Transfer for Facial Augmentation and Regeneration: Role of Mesenchymal Stem Cells","volume":"26","author":"Clauser","year":"2018","journal-title":"Atlas Oral. Maxillofac. Surg. Clin. N. Am."}],"container-title":["Clinics and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2039-7283\/13\/4\/85\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:27:39Z","timestamp":1760128059000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2039-7283\/13\/4\/85"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,8]]},"references-count":64,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["clinpract13040085"],"URL":"https:\/\/doi.org\/10.3390\/clinpract13040085","relation":{},"ISSN":["2039-7283"],"issn-type":[{"value":"2039-7283","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,8]]}}}