{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T14:53:04Z","timestamp":1793890384546,"version":"4.8.0"},"reference-count":37,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,28]],"date-time":"2024-04-28T00:00:00Z","timestamp":1714262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"INBRE PROGRAM NIH","award":["P20 GM103408"],"award-info":[{"award-number":["P20 GM103408"]}]},{"name":"National Institute of General Medical Sciences","award":["P20 GM103408"],"award-info":[{"award-number":["P20 GM103408"]}]},{"name":"College of Life Sciences at Brigham Young University","award":["P20 GM103408"],"award-info":[{"award-number":["P20 GM103408"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Oncology"],"abstract":"<jats:p>Personalized cancer care requires molecular characterization of neoplasms. While the research community accepts frozen tissues as the gold standard analyte for molecular assays, the source of tissue for testing in clinical cancer care comes almost universally from formalin-fixed, paraffin-embedded tissue (FFPE). As newer technologies emerge for DNA characterization that requires higher molecular weight DNA, it was necessary to compare the quality of DNA in terms of DNA length between FFPE and cryopreserved samples. We hypothesized that cryopreserved samples would yield higher quantity and superior quality DNA compared to FFPE samples. We analyzed DNA metrics by performing a head-to-head comparison between FFPE and cryopreserved samples from 38 human tumors representing various cancer types. DNA quantity and purity were measured by UV spectrophotometry, and DNA from cryopreserved tissue demonstrated a 4.2-fold increase in DNA yield per mg of tissue (p-value &lt; 0.001). DNA quality was measured on a fragment microelectrophoresis analyzer, and again, DNA from cryopreserved tissue demonstrated a 223% increase in the DNA quality number and a 9-fold increase in DNA fragments &gt; 40,000 bp (p-value &lt; 0.0001). DNA from the cryopreserved tissues was superior to the DNA from FFPE samples in terms of DNA yield and quality.<\/jats:p>","DOI":"10.3390\/curroncol31050183","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T03:43:46Z","timestamp":1714707826000},"page":"2441-2452","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["DNA Quantity and Quality Comparisons between Cryopreserved and FFPE Tumors from Matched Pan-Cancer Samples"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2283-3586","authenticated-orcid":false,"given":"Jeffrey","family":"Okojie","sequence":"first","affiliation":[{"name":"Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA"},{"name":"Department of Biomedical and Pharmaceutical Sciences, Idaho State University, Pocatello, ID 83209, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikole","family":"O\u2019Neal","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Pharmaceutical Sciences, Idaho State University, Pocatello, ID 83209, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mackenzie","family":"Burr","sequence":"additional","affiliation":[{"name":"Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peyton","family":"Worley","sequence":"additional","affiliation":[{"name":"Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac","family":"Packer","sequence":"additional","affiliation":[{"name":"Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DeLaney","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridger","family":"Kearns","sequence":"additional","affiliation":[{"name":"Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaniz","family":"Fatema","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Pharmaceutical Sciences, Idaho State University, Pocatello, ID 83209, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Dixon","sequence":"additional","affiliation":[{"name":"Specicare, 690 Medical Park Ln, Gainesville, GA 30501, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6059-9009","authenticated-orcid":false,"given":"Jared J.","family":"Barrott","sequence":"additional","affiliation":[{"name":"Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA"},{"name":"Department of Biomedical and Pharmaceutical Sciences, Idaho State University, Pocatello, ID 83209, USA"},{"name":"Specicare, 690 Medical Park Ln, Gainesville, GA 30501, USA"},{"name":"Simmons Center for Cancer Research, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ccell.2020.12.021","article-title":"The NCI-MATCH: A National, Collaborative Precision Oncology Trial for Diverse Tumor Histologies","volume":"39","author":"Drilon","year":"2021","journal-title":"Cancer Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1158\/1078-0432.CCR-22-3334","article-title":"National Cancer Institute Combination Therapy Platform Trial with Molecular Analysis for Therapy Choice (ComboMATCH)","volume":"29","author":"Ford","year":"2023","journal-title":"Clin. 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