{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T22:53:48Z","timestamp":1779231228696,"version":"3.51.4"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Fujian Province","award":["2022J05332"],"award-info":[{"award-number":["2022J05332"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Oncology"],"abstract":"<jats:p>Background and Aims: Cystatin B (CSTB) has been demonstrated to play a significant role in the pathogenesis of a number of diseases, including the evolution and progression of multiple cancers. Nevertheless, the function of CSTB in intrahepatic cholangiocarcinoma (iCCA) is yet to be fully elucidated. Methods: By analyzing transcriptome sequencing data from the FU-iCCA cohort, the iCCA-27 cohort, and three public databases, we identified genes associated with iCCA prognosis and selected CSTB as the subject of our study. The expression of CSTB was examined between tumor tissues and adjacent normal tissues obtained from iCCA patients via Western blot analysis. The clinical significance of CSTB was analyzed through immunohistochemical staining of a tissue microarray. Subsequently, the biological effects of CSTB overexpression or knockdown on iCCA cells were evaluated in vitro and in vivo. Results: CSTB expression was markedly elevated in the CCA pathological tissues in comparison to the corresponding adjacent normal tissues. A correlation was identified between higher CSTB expression and poorer patient prognosis in the analysis of 176 iCCA patients. It is noteworthy that overexpression or knockdown experiments demonstrated that CSTB plays a role in the proliferation, migration, and invasion of cells. In subcutaneous tumor models in nude mice, the knockdown of CSTB resulted in smaller tumors in terms of size and weight, and a slower growth rate. Conclusions: CSTB plays a significant function in the regulation of iCCA progression and may serve as a promising biomarker for iCCA.<\/jats:p>","DOI":"10.3390\/curroncol32020056","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T05:47:42Z","timestamp":1737438462000},"page":"56","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cystatin B Promotes the Proliferation, Migration, and Invasion of Intrahepatic Cholangiocarcinoma"],"prefix":"10.3390","volume":"32","author":[{"given":"Dai","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory for Carcinogenesis and Cancer Invasion (Ministry of Education), Department of Hepatic Oncology, Liver Cancer Institute, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China"},{"name":"Department of Hepatic Oncology, Xiamen Clinical Research Center for Cancer Therapy, Zhongshan Hospital, Fudan University (Xiamen Branch), Xiamen 361015, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bao-Ye","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-Fang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Hepatic Oncology, Xiamen Clinical Research Center for Cancer Therapy, Zhongshan Hospital, Fudan University (Xiamen Branch), Xiamen 361015, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhu-Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Carcinogenesis and Cancer Invasion (Ministry of Education), Department of Hepatic Oncology, Liver Cancer Institute, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China"},{"name":"Department of Liver Surgery and Transplantation, Zhongshan Hospital, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su-Su","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Hepatic Oncology, Xiamen Clinical Research Center for Cancer Therapy, Zhongshan Hospital, Fudan University (Xiamen Branch), Xiamen 361015, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Qiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory for Carcinogenesis and Cancer Invasion (Ministry of Education), Department of Hepatic Oncology, Liver Cancer Institute, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China"},{"name":"Department of Liver Surgery and Transplantation, Zhongshan Hospital, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu-Kang","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory for Carcinogenesis and Cancer Invasion (Ministry of Education), Department of Hepatic Oncology, Liver Cancer Institute, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China"},{"name":"Department of Liver Surgery and Transplantation, Zhongshan Hospital, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery and Transplantation, Zhongshan Hospital, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery and Transplantation, Zhongshan Hospital, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery and Transplantation, Zhongshan Hospital, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang-Jian","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery and Transplantation, Zhongshan Hospital, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo-Heng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hepatic Oncology, Xiamen Clinical Research Center for Cancer Therapy, Zhongshan Hospital, Fudan University (Xiamen Branch), Xiamen 361015, China"},{"name":"Center for Evidence-Based Medicine, Shanghai Medical School, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1002\/hep.31799","article-title":"The Co-mutational Spectrum Determines the 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