{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:23:00Z","timestamp":1785424980189,"version":"3.56.0"},"reference-count":55,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000008","name":"Health Canada","doi-asserted-by":"crossref","award":["2122-HQ-000066"],"award-info":[{"award-number":["2122-HQ-000066"]}],"id":[{"id":"10.13039\/501100000008","id-type":"DOI","asserted-by":"crossref"}]},{"award":["2122-HQ-000066"],"award-info":[{"award-number":["2122-HQ-000066"]}],"id":[{"id":"https:\/\/ror.org\/05p8nb362","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Bruyere Health Academic Medical Organization Innovation Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Oncology"],"abstract":"<jats:p>Opioids are first-line therapy for cancer pain, yet up to 30% of patients fail to achieve adequate control at standard doses. Opioid response is partly genetically mediated, and understanding these factors may improve symptom management. This project aimed to assess the feasibility of using tumor bank DNA for pharmacogenetic analyses and to validate previously identified genetic variants associated with opioid response using existing genetic and clinical data. In this retrospective cohort study, clinical data (morphine equivalent daily dose, demographics) and genetic data (single-nucleotide polymorphisms) were analyzed across 31 candidate loci. Adult deceased patients with melanoma, colorectal, or lung cancer treated with opioids between 2016 and 2021 and with available tumor bank DNA were included. Patients without sufficient DNA or not deceased were excluded. Of 3503 potential samples, 502 met the inclusion criteria. The median morphine equivalent daily dose was 40 mg (range 1\u20132140 mg). Eleven loci across six genes may be associated with higher (OPRM1, TAOK3, NFKBIA, COMT, and RHBDF2) and lower (COMT and GCH1) opioid dose requirements (p &lt; 0.05, not significant after Bonferroni correction). Ultimately, tumor bank DNA is a feasible resource for pharmacogenetic research. Identified loci may contribute to variability in opioid response and support future personalized pain management strategies.<\/jats:p>","DOI":"10.3390\/curroncol33060363","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T05:56:31Z","timestamp":1781675791000},"page":"363","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating the Use of Tumor Bank DNA to Validate Genetic Factors Impacting Opioid Response in Patients with Advanced Cancer"],"prefix":"10.3390","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6361-8815","authenticated-orcid":false,"given":"Christine L.","family":"Watt","sequence":"first","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"Bruy\u00e8re Health Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"Division of Palliative Care, Department of Medicine, University of Ottawa, Ottawa, ON K1R 6M1, Canada"},{"name":"The Ottawa Hospital, Ottawa, ON K1H 8L6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1904-3727","authenticated-orcid":false,"given":"Rebecca","family":"Lelievre","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"Bruy\u00e8re Health Research Institute, Ottawa, ON K1R 6M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3366-2565","authenticated-orcid":false,"given":"Gaelle Chopin Stukart","family":"Parsons","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"Bruy\u00e8re Health Research Institute, Ottawa, ON K1R 6M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3628-7418","authenticated-orcid":false,"given":"Caroline","family":"Vergette","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venus","family":"Chirip","sequence":"additional","affiliation":[{"name":"The Ottawa Hospital, Ottawa, ON K1H 8L6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Polskaia","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"Bruy\u00e8re Health Research Institute, Ottawa, ON K1R 6M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5358-5685","authenticated-orcid":false,"given":"Julie","family":"Lapenskie","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"Bruy\u00e8re Health Research Institute, Ottawa, ON K1R 6M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"Lo","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"The Ottawa Hospital, Ottawa, ON K1H 8L6, Canada"},{"name":"Department of Pathology and Laboratory Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pearl","family":"Campbell","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Bankapur","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gareth","family":"Palidwor","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7479-1560","authenticated-orcid":false,"given":"James","family":"Downar","sequence":"additional","affiliation":[{"name":"Ottawa Hospital Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"Bruy\u00e8re Health Research Institute, Ottawa, ON K1R 6M1, Canada"},{"name":"Division of Palliative Care, Department of Medicine, University of Ottawa, Ottawa, ON K1R 6M1, Canada"},{"name":"The Ottawa Hospital, Ottawa, ON K1H 8L6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"iv166","DOI":"10.1093\/annonc\/mdy152","article-title":"Management of Cancer Pain in Adult Patients: ESMO Clinical Practice Guidelines","volume":"29","author":"Fallon","year":"2018","journal-title":"Ann. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0304-3959(01)00324-4","article-title":"Symptoms during Cancer Pain Treatment Following WHO-Guidelines: A Longitudinal Follow-up Study of Symptom Prevalence, Severity and Etiology","volume":"93","author":"Meuser","year":"2001","journal-title":"Pain"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"833","DOI":"10.2217\/fon-2016-0423","article-title":"Cancer Pain: A Review of Epidemiology, Clinical Quality and Value Impact","volume":"13","author":"Neufeld","year":"2017","journal-title":"Future Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.2147\/JPR.S139715","article-title":"Adequacy of Cancer-Related Pain Management and Predictors of Undertreatment at Referral to a Pain Clinic","volume":"10","author":"Lawlor","year":"2017","journal-title":"J. Pain Res."},{"key":"ref_5","first-page":"379","article-title":"Pain and Its Interference with Daily Activities in Medical Oncology Outpatients","volume":"16","author":"Burger","year":"2013","journal-title":"Pain Physician"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1093\/annonc\/mdm056","article-title":"Prevalence of Pain in Patients with Cancer: A Systematic Review of the Past 40 Years","volume":"18","author":"Kessels","year":"2007","journal-title":"Ann. Oncol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e58","DOI":"10.1016\/S1470-2045(12)70040-2","article-title":"Use of Opioid Analgesics in the Treatment of Cancer Pain: Evidence-Based Recommendations from the EAPC","volume":"13","author":"Caraceni","year":"2012","journal-title":"Lancet Oncol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4149","DOI":"10.1200\/JCO.2014.56.0383","article-title":"Quality of Cancer Pain Management: An Update of a Systematic Review of Undertreatment of Patients with Cancer","volume":"32","author":"Greco","year":"2014","journal-title":"J. Clin. Oncol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1016\/j.jpainsymman.2019.06.017","article-title":"Cancer-Related Pain: A Longitudinal Study of Time to Stable Pain Control and Its Clinicodemographic Predictors","volume":"58","author":"Sabri","year":"2019","journal-title":"J. Pain Symptom Manag."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.clon.2011.04.015","article-title":"Evolving Knowledge of Opioid Genetics in Cancer Pain","volume":"23","author":"Droney","year":"2011","journal-title":"Clin. Oncol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1016\/j.pain.2012.02.022","article-title":"Pain Sensitivity and Opioid Analgesia: A Pharmacogenomic Twin Study","volume":"153","author":"Angst","year":"2012","journal-title":"Pain"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1002\/cpt.2149","article-title":"Clinical Pharmacogenetics Implementation Consortium Guideline for CYP2D6, OPRM1, and COMT Genotypes and Select Opioid Therapy","volume":"110","author":"Crews","year":"2021","journal-title":"Clin. Pharmacol. Ther."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1002\/phar.1986","article-title":"Review of Opioid Pharmacogenetics and Considerations for Pain Management","volume":"37","author":"Hamadeh","year":"2017","journal-title":"Pharmacotherapy"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1002\/jcph.1873","article-title":"Pharmacogenomics and Morphine","volume":"61","author":"Ofoegbu","year":"2021","journal-title":"J. Clin. Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"116897","DOI":"10.1016\/j.biopha.2024.116897","article-title":"ABCB1 Genetic Polymorphisms Affect Opioid Requirement by Altering Function of the Intestinal P-Glycoprotein","volume":"176","author":"Qin","year":"2024","journal-title":"Biomed. Pharmacother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/sj.clpt.6100385","article-title":"Association of ABCB1\/MDR1 and OPRM1 Gene Polymorphisms with Morphine Pain Relief","volume":"83","author":"Campa","year":"2008","journal-title":"Clin. Pharmacol. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.soncn.2019.04.011","article-title":"Genetic Variants Associated with Cancer Pain and Response to Opioid Analgesics: Implications for Precision Pain Management","volume":"35","author":"Yang","year":"2019","journal-title":"Semin. Oncol. Nurs."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1111\/j.1399-6576.2004.00517.x","article-title":"The 118 A > G Polymorphism in the Human Mu-Opioid Receptor Gene May Increase Morphine Requirements in Patients with Pain Caused by Malignant Disease","volume":"48","author":"Klepstad","year":"2004","journal-title":"Acta Anaesthesiol. Scand."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2937","DOI":"10.7314\/APJCP.2013.14.5.2937","article-title":"Gene Polymorphisms of OPRM1 A118G and ABCB1 C3435T May Influence Opioid Requirements in Chinese Patients with Cancer Pain","volume":"14","author":"Gong","year":"2013","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1089\/jpm.2024.0112","article-title":"Influence of COMT (Rs4680) and OPRM1 (Rs1799971) on Cancer Pain, Opioid Dose, and Adverse Effects","volume":"27","author":"Wong","year":"2024","journal-title":"J. Palliat. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.2217\/pgs-2017-0060","article-title":"Advanced Cancer Pain: The Search for Genetic Factors Correlated with Interindividual Variability in Opioid Requirement","volume":"18","author":"Matic","year":"2017","journal-title":"Pharmacogenomics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1016\/j.pain.2011.01.040","article-title":"Influence from Genetic Variability on Opioid Use for Cancer Pain: A European Genetic Association Study of 2294 Cancer Pain Patients","volume":"152","author":"Klepstad","year":"2011","journal-title":"Pain"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.jpain.2009.04.001","article-title":"Genetic Contributions to Clinical Pain and Analgesia: Avoiding Pitfalls in Genetic Research","volume":"10","author":"Kim","year":"2009","journal-title":"J. Pain"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Nishizawa, D., Terui, T., Ishitani, K., Kasai, S., Hasegawa, J., Nakayama, K., Ebata, Y., and Ikeda, K. (2022). Genome-Wide Association Study Identifies Candidate Loci Associated with Opioid Analgesic Requirements in the Treatment of Cancer Pain. Cancers, 14.","DOI":"10.3390\/cancers14194692"},{"key":"ref_25","unstructured":"(2023, July 03). College of Physicians and Surgeons of British Columbia Methadone for Analgesia Guidelines. Available online: https:\/\/www.cpsbc.ca\/registrants\/programs\/drug-programs\/methadone."},{"key":"ref_26","unstructured":"(2023, July 03). BC Center for Palliative Care Inter-Professional Palliative Symptom Management Guidelines. Available online: https:\/\/www.bc-cpc.ca\/symptom-management-guidelines\/."},{"key":"ref_27","unstructured":"(2025, December 12). AmpliSeq for Illumina Sequencing Solution. Available online: https:\/\/www.illumina.com\/products\/by-brand\/ampliseq.html."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1136\/bmjspcare-2012-000396","article-title":"Factors Affecting Recruitment to an Observational Multicentre Palliative Care Study","volume":"3","author":"Stone","year":"2013","journal-title":"BMJ Support. Palliat. Care"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1177\/09697330251356550","article-title":"Recruiting Participants for Palliative Care Research: A Reflective Discussion Paper","volume":"32","author":"McCarthy","year":"2025","journal-title":"Nurs. Ethics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"112060","DOI":"10.1016\/j.biopha.2021.112060","article-title":"Reviewing Pharmacogenetics to Advance Precision Medicine for Opioids","volume":"142","author":"Magarbeh","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/s11916-026-01485-5","article-title":"The OPRM1 A118G Polymorphism Revisited: Recent Developments and Implications for Personalized Treatment","volume":"30","author":"Daybell","year":"2026","journal-title":"Curr. Pain Headache Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.pain.2009.10.021","article-title":"A GTP Cyclohydrolase 1 Genetic Variant Delays Cancer Pain","volume":"148","author":"Klepstad","year":"2010","journal-title":"Pain"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.jpainsymman.2018.07.011","article-title":"Single-Nucleotide Polymorphisms in TAOK3 Are Associated with High Opioid Requirement for Pain Management in Patients with Advanced Cancer Admitted to a Tertiary Palliative Care Unit","volume":"56","author":"Gutteridge","year":"2018","journal-title":"J. Pain Symptom Manag."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1097\/j.pain.0000000000000946","article-title":"Pain versus Analgesia: TAOK3 as a Pharmacogene","volume":"158","author":"Li","year":"2017","journal-title":"Pain"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2636","DOI":"10.1158\/1055-9965.EPI-09-0426","article-title":"The Role of Inflammation Gene Polymorphisms on Pain Severity in Lung Cancer Patients","volume":"18","author":"Spitz","year":"2009","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.1111\/j.0953-816X.2004.03441.x","article-title":"Spinal NF-kB Activation Induces COX-2 Upregulation and Contributes to Inflammatory Pain Hypersensitivity","volume":"19","author":"Lee","year":"2004","journal-title":"Eur. J. Neurosci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4581","DOI":"10.1158\/1078-0432.CCR-10-3028","article-title":"Multiple Loci Modulate Opioid Therapy Response for Cancer Pain","volume":"17","author":"Galvan","year":"2011","journal-title":"Clin. Cancer Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.pain.2005.03.032","article-title":"The Val158Met Polymorphism of the Human Catechol-O-Methyltransferase (COMT) Gene May Influence Morphine Requirements in Cancer Pain Patients","volume":"116","author":"Klepstad","year":"2005","journal-title":"Pain"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"380","DOI":"10.3892\/br.2017.963","article-title":"Prospective Replication Study Implicates the Catechol-O-Methyltransferase Val158Met Polymorphism as a Biomarker for the Response to Morphine in Patients with Cancer","volume":"7","author":"Matsuoka","year":"2017","journal-title":"Biomed. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Hajj, A., Halepian, L., Osta, N.E., Chahine, G., Kattan, J., and Rabbaa Khabbaz, L. (2017). OPRM1 c.118A > G Polymorphism and Duration of Morphine Treatment Associated with Morphine Doses and Quality-of-Life in Palliative Cancer Pain Settings. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18040669"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1016\/j.jpain.2014.09.002","article-title":"How Much Oxycodone Is Needed for Adequate Analgesia after Breast Cancer Surgery: Effect of the OPRM1 118A > G Polymorphism","volume":"15","author":"Cajanus","year":"2014","journal-title":"J. Pain"},{"key":"ref_42","first-page":"64","article-title":"Genetic Variation in the Catechol-O-Methyltransferase (COMT) Gene and Morphine Requirements in Cancer Patients with Pain","volume":"4","author":"Ross","year":"2008","journal-title":"Mol. Pain"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.jpainsymman.2021.03.024","article-title":"Genetic Factors Associated With Pain Severity, Daily Opioid Dose Requirement, and Pain Response Among Advanced Cancer Patients Receiving Supportive Care","volume":"62","author":"Yennurajalingam","year":"2021","journal-title":"J. Pain Symptom Manag."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Ferreira, D., Barratt, D., Somogyi, A., and Currow, D. (2020). Genetics of Response to Sustained-Release Morphine for Chronic Breathlessness\u2014An Exploratory Study. Eur. Respir. J., 56.","DOI":"10.1183\/13993003.congress-2020.183"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3262","DOI":"10.1158\/1055-9965.EPI-08-0125","article-title":"The Influence of Tumor Necrosis Factor-Alpha -308 G\/A and IL-6 -174 G\/C on Pain and Analgesia Response in Lung Cancer Patients Receiving Supportive Care","volume":"17","author":"Spitz","year":"2008","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Benchimol, E.I., Smeeth, L., Guttmann, A., Harron, K., Moher, D., Petersen, I., S\u00f8rensen, H.T., von Elm, E., and Langan, S.M. (2015). RECORD Working Committee The REporting of Studies Conducted Using Observational Routinely-Collected Health Data (RECORD) Statement. PLoS Med., 12.","DOI":"10.1371\/journal.pmed.1001885"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"152249","DOI":"10.1016\/j.anndiagpath.2023.152249","article-title":"Challenges to the Effectiveness of Next-Generation Sequencing in Formalin-Fixed Paraffin-Embedded Tumor Samples for Non-Small Cell Lung Cancer","volume":"69","author":"Viola","year":"2024","journal-title":"Ann. Diagn. Pathol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1007\/s40122-025-00728-4","article-title":"The Use of Opioids in an Acute Palliative Care Unit to Re-Assess Prescriptions","volume":"14","author":"Mercadante","year":"2025","journal-title":"Pain Ther."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1744806920908474","DOI":"10.1177\/1744806920908474","article-title":"COMT Gene Variants and \u03b2-Endorphin Levels Contribute to Ethnic Differences in Experimental Pain Sensitivity","volume":"16","author":"Xu","year":"2020","journal-title":"Mol. Pain"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1042\/BJ20111050","article-title":"A Common Single Nucleotide Polymorphism A118g of the Mu Opioid Receptor Alters Its N-Glycosylation and Protein Stability","volume":"441","author":"Huang","year":"2012","journal-title":"Biochem. J."},{"key":"ref_51","unstructured":"Government of Canada (2026, January 13). 2021 Census of Population Geographic Summary: Ottawa, City (CV) [Census Subdivision], Ontario, Available online: https:\/\/www12.statcan.gc.ca\/census-recensement\/2021\/search-recherche\/productresults-resultatsproduits-eng.cfm."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1002\/humu.24309","article-title":"Variant Interpretation Using Population Databases: Lessons from gnomAD","volume":"43","author":"Gudmundsson","year":"2022","journal-title":"Hum. Mutat."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"873","DOI":"10.2217\/14622416.6.8.873","article-title":"Concordance of Pharmacogenetic Markers in Germline and Colorectal Tumor DNA","volume":"6","author":"Marsh","year":"2005","journal-title":"Pharmacogenomics"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"489","DOI":"10.2217\/14622416.10.3.489","article-title":"Using Germline Genotype in Cancer Pharmacogenetic Studies","volume":"10","author":"McWhinney","year":"2009","journal-title":"Pharmacogenomics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1200\/PO.21.00279","article-title":"Germline Testing Data Validate Inferences of Mutational Status for Variants Detected From Tumor-Only Sequencing","volume":"5","author":"Jalloul","year":"2021","journal-title":"JCO Precis. Oncol."}],"container-title":["Current Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1718-7729\/33\/6\/363\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T04:51:09Z","timestamp":1781844669000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1718-7729\/33\/6\/363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,17]]},"references-count":55,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["curroncol33060363"],"URL":"https:\/\/doi.org\/10.3390\/curroncol33060363","relation":{},"ISSN":["1718-7729"],"issn-type":[{"value":"1718-7729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,17]]}}}