{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:11:30Z","timestamp":1788858690013,"version":"build-2803163510"},"reference-count":88,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatopathology"],"abstract":"<jats:p>Skin biopsy is one of the most valuable diagnostic procedures in dermatology, particularly when clinical findings alone are insufficient to establish a diagnosis. However, obtaining an accurate histopathological diagnosis depends on multiple steps, and errors at any stage of the biopsy pathway may compromise the final result. This review synthesizes current evidence and available guidelines on best practices for skin biopsy, integrating the practical experience of four internationally recognized dermatopathologists to address areas where evidence is limited or poorly standardized. The review covers biopsy planning, selection of the optimal biopsy site and technique, specimen handling and fixation, grossing and laboratory processing, prevention of technical artifacts, the use of ancillary diagnostic techniques, and clinicopathological correlation, with particular attention to challenging anatomical sites and complex diseases. Diagnostic accuracy depends on obtaining a representative specimen, maintaining high technical standards throughout tissue processing, providing adequate clinical information, and ensuring close communication between the clinician and the dermatopathologist. In selected cases, multidisciplinary review is required to reach a definitive diagnosis. Adherence to these principles can optimize diagnostic yield, reduce avoidable errors, and ultimately improve patient care.<\/jats:p>","DOI":"10.3390\/dermatopathology13030042","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:10:22Z","timestamp":1788855022000},"page":"42","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Basic Principles of Skin Biopsy Optimization in Dermatopathology"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1187-1341","authenticated-orcid":false,"given":"Mar","family":"Llamas-Velasco","sequence":"first","affiliation":[{"name":"Department of Dermatology, Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Rozas-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Hospital San Pablo, Coquimbo 1781881, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6617-2364","authenticated-orcid":false,"given":"Angel","family":"Fernandez-Flores","sequence":"additional","affiliation":[{"name":"Department of Cellular Pathology, Hospital Universitario El Bierzo, 24404 Ponferrada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2366-7110","authenticated-orcid":false,"given":"Maria-Teresa","family":"Fernandez-Figueras","sequence":"additional","affiliation":[{"name":"Department of Anatomic Pathology, Hospital Universitari General de Catalunya, Grupo Quironsalud, Sant Cugat del Vall\u00e9s, 08195 Barcelona, Spain"},{"name":"Department of Medicine, Faculty of Medicine and Health Science, Universitat Internacional de Catalunya, Sant Cugat del Vall\u00e9s, 08195 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"ref_1","first-page":"193","article-title":"Derm Dotting: A New Technique That Improves Diagnostic Precision in the Evaluation of Skin Lesions","volume":"110","year":"2019","journal-title":"Actas Dermosifiliogr. (Engl. Ed.)"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.jaad.2010.12.031","article-title":"Clinicopathological consistency in skin disorders: A retrospective study of 3949 pathological reports","volume":"66","author":"Aslan","year":"2012","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1001\/jamadermatol.2013.7227","article-title":"Nonspecific histopathological diagnoses: The impact of partial biopsy and the need for a consensus guideline","volume":"150","author":"Sellheyer","year":"2014","journal-title":"JAMA Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4103\/0974-2077.44174","article-title":"Techniques of Skin Biopsy and Practical Considerations","volume":"1","author":"Nischal","year":"2008","journal-title":"J. Cutan. Aesthet. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.jaad.2004.11.072","article-title":"A retrospective biopsy study of the clinical diagnostic accuracy of common skin diseases by different specialties compared with dermatology","volume":"52","author":"Sellheyer","year":"2005","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_6","first-page":"12","article-title":"Basic concepts in skin biopsy. Part I","volume":"103","author":"Paredes","year":"2012","journal-title":"Actas Dermosifiliogr."},{"key":"ref_7","first-page":"93","article-title":"Aseguramiento de la calidad en el laboratorio de Anatom\u00eda Patol\u00f3gica","volume":"28","author":"Tresserra","year":"2015","journal-title":"Rev. Senol. Patol. Mamar."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Steimetz, E., Mostafidi, E., Castagna, C., Gupta, R., and Frasso, R. (2024). Forgotten clientele: A systematic review of patient-centered pathology reports. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0301116"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.jaad.2012.10.058","article-title":"Medical error in dermatology practice: Development of a classification system to drive priority setting in patient safety efforts","volume":"68","author":"Watson","year":"2013","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1111\/ijd.12330","article-title":"Provider-to-provider communication in dermatology and implications of missing clinical information in skin biopsy requisition forms: A systematic review","volume":"53","author":"Comfere","year":"2014","journal-title":"Int. J. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.jaad.2004.11.073","article-title":"\u201cLesion,\u201d \u201crule out\u2026,\u201d and other vagaries of filling out pathology requisition forms","volume":"52","author":"Sellheyer","year":"2005","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.jaad.2009.09.009","article-title":"Influence of evaluation of clinical pictures on the histopathologic diagnosis of inflammatory skin disorders","volume":"63","author":"Cerroni","year":"2010","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1001\/jamadermatol.2024.4281","article-title":"Impact of Clinical Information on Melanocytic Skin Lesion Pathology Diagnosis: A Scoping Review","volume":"160","author":"Lai","year":"2024","journal-title":"JAMA Dermatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1097\/DAD.0000000000002563","article-title":"Approach to the so-called \u201cInvisible Dermatosis\u201d: When Subtle Histopathological Findings Guide Diagnosis","volume":"45","author":"Ballester","year":"2023","journal-title":"Am. J. Dermatopathol."},{"key":"ref_15","first-page":"337","article-title":"Topical Corticosteroids: Choice and Application","volume":"103","author":"Stacey","year":"2021","journal-title":"Am. Fam. Physician"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jdcr.2021.05.036","article-title":"A case of secondary syphilis masquerading as cutaneous lymphoma","volume":"14","author":"Nethers","year":"2021","journal-title":"JAAD Case Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1097\/DAD.0000000000000766","article-title":"Occlusive Nonvasculitic Vasculopathy","volume":"39","author":"Cerroni","year":"2017","journal-title":"Am. J. Dermatopathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1097\/DAD.0000000000003117","article-title":"Not So Normal Skin: A Review of the \u201cRandom\u201d Skin Biopsy","volume":"48","author":"Milam","year":"2026","journal-title":"Am. J. Dermatopathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1111\/jdv.70249","article-title":"Recommendations for the diagnostic work-up of cutaneous small vessel vasculitis\u2014Position Statement of the European Academy of Dermatology and Venereology Vasculitis and Vasculopathy Task Force","volume":"40","author":"Alpsoy","year":"2026","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jaad.2015.06.033","article-title":"Skin biopsy: Biopsy issues in specific diseases","volume":"74","author":"Elston","year":"2016","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/j.1600-0560.2008.01175.x","article-title":"Skin biopsy for inflammatory and common neoplastic skin diseases: Optimum time, best location and preferred techniques. A critical review","volume":"36","author":"Sina","year":"2009","journal-title":"J. Cutan. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1111\/j.1524-4725.1992.tb02000.x","article-title":"Nail biopsy. Indications and methods","volume":"18","author":"Rich","year":"1992","journal-title":"J. Dermatol. Surg. Oncol."},{"key":"ref_23","first-page":"100","article-title":"Basic concepts in skin biopsy. Part II","volume":"103","author":"Paredes","year":"2012","journal-title":"Actas Dermosifiliogr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/cup.14423","article-title":"An examination of clinical and laboratory practices that introduce diagnostic error in biopsies for alopecia","volume":"50","author":"Wiggins","year":"2023","journal-title":"J. Cutan. Pathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"S16","DOI":"10.1016\/j.jaad.2023.05.047","article-title":"The role of the scalp biopsy in the evaluation of alopecia","volume":"89","author":"Sperling","year":"2023","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/j.jaad.2016.01.022","article-title":"Guidelines for the use of local anesthesia in office-based dermatologic surgery","volume":"74","author":"Kouba","year":"2016","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_27","first-page":"995","article-title":"Shave and punch biopsy for skin lesions","volume":"84","author":"Pickett","year":"2011","journal-title":"Am. Fam. Physician"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1001\/archderm.143.8.1074","article-title":"Histologic cutaneous modifications after the use of EMLA cream, a diagnostic pitfall: Review of 13 cases","volume":"143","author":"Cazes","year":"2007","journal-title":"Arch. Dermatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e94","DOI":"10.1111\/pde.12047","article-title":"Cutaneous Histologic Artifact Associated with the Use of a Needle-Free Anesthesia Device for Skin Biopsy","volume":"30","author":"Smith","year":"2013","journal-title":"Pediatr. Dermatol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/cup.14724","article-title":"Incidental acantholysis in a case of bullous pemphigoid-A diagnostic quandary","volume":"52","author":"Batrani","year":"2025","journal-title":"J. Cutan. Pathol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1097\/DAD.0b013e31827f0d27","article-title":"Routine use of ex vivo dermoscopy with \u201cderm dotting\u201d in dermatopathology","volume":"35","author":"Haspeslagh","year":"2013","journal-title":"Am. J. Dermatopathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1002\/ca.70005","article-title":"Dissecting the Impact of Anatomy Lab Policies on Pregnant Medical Students","volume":"38","author":"Wozniak","year":"2025","journal-title":"Clin. Anat."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/modpathol.3800354","article-title":"Ultrasound-accelerated formalin fixation of tissue improves morphology, antigen and mRNA preservation","volume":"18","author":"Chu","year":"2005","journal-title":"Mod. Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Berrino, E., Annaratone, L., Miglio, U., Maldi, E., Piccinelli, C., Peano, E., Balmativola, D., Cassoni, P., Pisacane, A., and Sarotto, I. (2020). Cold Formalin Fixation Guarantees DNA Integrity in Formalin Fixed Paraffin Embedded Tissues: Premises for a Better Quality of Diagnostic and Experimental Pathology with a Specific Impact on Breast Cancer. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.00173"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"170","DOI":"10.3109\/10520295.2012.754496","article-title":"Preservation of immunorecognition by transferring cells from 10% neutral buffered formalin to 70% ethanol","volume":"88","author":"Otali","year":"2013","journal-title":"Biotech. Histochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/1523-1747.ep12627611","article-title":"Preservation of tissue-fixed immunoglobulins in skin biopsies of patients with lupus erythematosus and bullous diseases\u2014Preliminary report","volume":"59","author":"Michel","year":"1972","journal-title":"J. Investig. Dermatol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/j.jaad.2016.11.006","article-title":"Direct immunofluorescence microscopy of skin biopsy samples preserved in honey","volume":"76","author":"Rao","year":"2017","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1111\/j.1524-4725.1991.tb03274.x","article-title":"Rapid skin edge elevation using the OCT compound droplet technique to obtain horizontal microsections in Mohs micrographic surgery","volume":"17","author":"Dogan","year":"1991","journal-title":"J. Dermatol. Surg. Oncol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1111\/j.1600-0560.2009.01492.x","article-title":"Formalin deposition as artifact in biopsies from patients affected by a new variant of endemic pemphigus foliaceus in El Bagre, Colombia, South America","volume":"37","author":"Howard","year":"2010","journal-title":"J. Cutan. Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"30","DOI":"10.32074\/1591-951X-1773","article-title":"Automated paraffin embedding of tissue samples significantly reduces overall workflow time in routine pathology practice","volume":"118","author":"Gambella","year":"2026","journal-title":"Pathologica"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1111\/cup.70090","article-title":"Follicular Porokeratosis Limited to the Scalp is Associated with Alopecia. A Case Report","volume":"53","author":"Farishta","year":"2026","journal-title":"J. Cutan. Pathol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"107","DOI":"10.5858\/2007-131-107-PSSFSS","article-title":"Prospective step sections for small skin biopsies","volume":"131","author":"Bruecks","year":"2007","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1600-0560.1998.tb01711.x","article-title":"Utility of deeper sections and special stains for dermatopathology specimens","volume":"25","author":"Maingi","year":"1998","journal-title":"J. Cutan. Pathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/cup.12267","article-title":"Utility of additional tissue sections in dermatopathology: Diagnostic, clinical and financial implications","volume":"41","author":"Stuart","year":"2014","journal-title":"J. Cutan. Pathol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1001\/archderm.136.4.471","article-title":"Utility of step sections: Demonstration of additional pathological findings in biopsy samples initially diagnosed as actinic keratosis","volume":"136","author":"Carag","year":"2000","journal-title":"Arch. Dermatol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"El Sharouni, M.-A., van Diest, P.J., and Blokx, W.A.M. (2022). Superficial basal cell carcinoma, think deeper: Step sectioning of skin biopsy specimens yields 14% more aggressive subtypes. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0256149"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.jaad.2015.06.034","article-title":"Skin biopsy: Identifying and overcoming errors in the skin biopsy pathway","volume":"74","author":"Stratman","year":"2016","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"279","DOI":"10.4103\/jomfp.JOMFP_125_15","article-title":"A review of artifacts in histopathology","volume":"22","author":"Taqi","year":"2018","journal-title":"J. Oral Maxillofac. Pathol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"S111","DOI":"10.4103\/0973-029X.141346","article-title":"Artefacts in histopathology","volume":"18","author":"Chatterjee","year":"2014","journal-title":"J. Oral Maxillofac. Pathol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1097\/DAD.0b013e31819b3e0c","article-title":"The value of skin biopsy in inflammatory dermatoses","volume":"31","author":"Rajaratnam","year":"2009","journal-title":"Am. J. Dermatopathol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1016\/j.jaci.2018.11.042","article-title":"Psoriatic skin molecular and histopathologic profiles after treatment with risankizumab versus ustekinumab","volume":"143","author":"Visvanathan","year":"2019","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1097\/DAD.0b013e3181cb3ff7","article-title":"Histopathologic spectrum of psoriasiform skin reactions associated with tumor necrosis factor-\u03b1 inhibitor therapy. A study of 16 biopsies","volume":"32","author":"Laga","year":"2010","journal-title":"Am. J. Dermatopathol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.jaad.2009.01.038","article-title":"Atypical histologic and immunohistochemical findings in melanocytic nevi after liquid nitrogen cryotherapy","volume":"61","author":"Adeniran","year":"2009","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1007\/s00403-023-02672-9","article-title":"A practical guide to clinical photography prior to skin biopsy: Key tips and proposed workflow","volume":"315","author":"Kim","year":"2023","journal-title":"Arch. Dermatol. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/j.jaad.2016.08.024","article-title":"Validation of diagnostic accuracy with whole-slide imaging compared with glass slide review in dermatopathology","volume":"75","author":"Shah","year":"2016","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4103\/2153-3539.93399","article-title":"Virtual microscopy using whole-slide imaging as an enabler for teledermatopathology: A paired consultant validation study","volume":"3","author":"Evans","year":"2012","journal-title":"J. Pathol. Inform."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s10278-015-9836-y","article-title":"Use of Digital Whole Slide Imaging in Dermatopathology","volume":"29","author":"Onega","year":"2016","journal-title":"J. Digit. Imaging"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1111\/cup.13954","article-title":"Artificial intelligence in dermatopathology: Diagnosis, education, and research","volume":"48","author":"Wells","year":"2021","journal-title":"J. Cutan. Pathol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1097\/DAD.0000000000002593","article-title":"Artificial Intelligence in Melanoma Dermatopathology: A Review of Literature","volume":"46","author":"Neimy","year":"2024","journal-title":"Am. J. Dermatopathol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1111\/jdv.20781","article-title":"Diagnosis melanoma with artificial intelligence systems: A meta-analysis study and systematic review","volume":"39","author":"Korkmaz","year":"2025","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1038\/s41591-020-0942-0","article-title":"Human-computer collaboration for skin cancer recognition","volume":"26","author":"Tschandl","year":"2020","journal-title":"Nat. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.annonc.2019.10.013","article-title":"Man against machine reloaded: Performance of a market-approved convolutional neural network in classifying a broad spectrum of skin lesions in comparison with 96 dermatologists working under less artificial conditions","volume":"31","author":"Haenssle","year":"2020","journal-title":"Ann. Oncol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/nature21056","article-title":"Dermatologist-level classification of skin cancer with deep neural networks","volume":"542","author":"Esteva","year":"2017","journal-title":"Nature"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"King, A.D., Deirawan, H., Klein, P.A., Dasgeb, B., Dumur, C.I., and Mehregan, D.R. (2023). Next-generation sequencing in dermatology. Front. Med., 10.","DOI":"10.3389\/fmed.2023.1218404"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1146\/annurev-pathol-012513-104658","article-title":"The molecular pathology of melanoma: An integrated taxonomy of melanocytic neoplasia","volume":"9","author":"Bastian","year":"2014","journal-title":"Annu. Rev. Pathol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Fujii, K., and Kanekura, T. (2019). Next-Generation Sequencing Technologies for Early-Stage Cutaneous T-Cell Lymphoma. Front. Med., 6.","DOI":"10.3389\/fmed.2019.00181"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"308ra158","DOI":"10.1126\/scitranslmed.aaa9122","article-title":"TCR sequencing facilitates diagnosis and identifies mature T cells as the cell of origin in CTCL","volume":"7","author":"Kirsch","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2672","DOI":"10.1182\/blood-2016-01-690636","article-title":"Revised classification of histiocytoses and neoplasms of the macrophage-dendritic cell lineages","volume":"127","author":"Emile","year":"2016","journal-title":"Blood"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.sder.2012.08.002","article-title":"Molecular diagnostics in genodermatoses","volume":"31","author":"Schaffer","year":"2012","journal-title":"Semin. Cutan. Med. Surg."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1111\/bjd.19384","article-title":"A decade of next-generation sequencing in genodermatoses: The impact on gene discovery and clinical diagnostics","volume":"184","author":"Chiu","year":"2021","journal-title":"Br. J. Dermatol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1038\/s41379-018-0168-6","article-title":"ALK-positive histiocytosis: An expanded clinicopathologic spectrum and frequent presence of KIF5B-ALK fusion","volume":"32","author":"Chang","year":"2019","journal-title":"Mod. Pathol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1182\/blood.2021013338","article-title":"ALK-positive histiocytosis: A new clinicopathologic spectrum highlighting neurologic involvement and responses to ALK inhibition","volume":"139","author":"Kemps","year":"2022","journal-title":"Blood"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.1038\/ncomms4116","article-title":"Kinase fusions are frequent in Spitz tumors and spitzoid melanomas","volume":"5","author":"Wiesner","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Figueras-Nart, I., Mascar\u00f3, J.M., Solanich, X., and Hern\u00e1ndez-Rodr\u00edguez, J. (2019). Dermatologic and Dermatopathologic Features of Monogenic Autoinflammatory Diseases. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.02448"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"202","DOI":"10.3390\/dermatopathology8020026","article-title":"Histologic Patterns and Clues to Autoinflammatory Diseases in Children: What a Cutaneous Biopsy Can Tell Us","volume":"8","author":"Kolivras","year":"2021","journal-title":"Dermatopathology"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1684\/ejd.2019.3539","article-title":"Targeted therapies and precision medicine for inflammatory skin diseases","volume":"29","author":"Gilliet","year":"2019","journal-title":"Eur. J. Dermatol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"100317","DOI":"10.1016\/j.xjidi.2024.100317","article-title":"Spatial Transcriptomics in Inflammatory Skin Diseases Using GeoMx Digital Spatial Profiling: A Practical Guide for Applications in Dermatology","volume":"5","author":"Cho","year":"2025","journal-title":"JID Innov."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"7729","DOI":"10.1038\/s41467-022-35319-w","article-title":"Spatial transcriptomics landscape of lesions from non-communicable inflammatory skin diseases","volume":"13","author":"Hillig","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"5970","DOI":"10.1158\/0008-5472.CAN-18-0747","article-title":"Spatially Resolved Transcriptomics Enables Dissection of Genetic Heterogeneity in Stage III Cutaneous Malignant Melanoma","volume":"78","author":"Thrane","year":"2018","journal-title":"Cancer Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1016\/j.jid.2023.02.040","article-title":"Single-Cell and Spatial Transcriptomic Analysis of Human Skin Delineates Intercellular Communication and Pathogenic Cells","volume":"143","author":"Thrane","year":"2023","journal-title":"J. Investig. Dermatol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1111\/exd.14827","article-title":"Spatial transcriptomics in human skin research","volume":"32","author":"Tekkela","year":"2023","journal-title":"Exp. Dermatol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1309\/AJCPHPG6DQFBKKUR","article-title":"Dermatopathologists\u2019 opinions about the quality of clinical information in the skin biopsy requisition form and the skin biopsy care process: A semiqualitative assessment","volume":"143","author":"Wong","year":"2015","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Chergui, M., Redpath, M., Wang, C.S., Mlynarek, A., Esfahani, K., Thibaudeau, S., Sultanem, K., and Claveau, J. (2025). Guidelines for Clinicians and dermatopathologists on Performing Skin Biopsies and Reporting on Suspected Cutaneous Squamous Cell Carcinoma. Curr. Oncol., 32.","DOI":"10.3390\/curroncol32120689"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/0190-9622(93)70106-4","article-title":"Diagnostic and predictive value of horizontal sections of scalp biopsy specimens in male pattern androgenetic alopecia","volume":"28","author":"Whiting","year":"1993","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Alpsoy, E. (2022). Cutaneous vasculitis; An algorithmic approach to diagnosis. Front. Med., 9.","DOI":"10.3389\/fmed.2022.1012554"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1365-2559.2009.03443.x","article-title":"The histological assessment of cutaneous vasculitis","volume":"56","author":"Carlson","year":"2010","journal-title":"Histopathology"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"52","DOI":"10.3390\/dermatopathology11010006","article-title":"Direct Immunofluorescence of Skin and Oral Mucosa: Guidelines for Selecting the Optimum Biopsy Site","volume":"11","author":"Mahmood","year":"2024","journal-title":"Dermatopathology"},{"key":"ref_88","first-page":"S33","article-title":"Direct Immunofluorescence","volume":"16","author":"Robinson","year":"2023","journal-title":"J. Clin. Aesthet. Dermatol."}],"container-title":["Dermatopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2296-3529\/13\/3\/42\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:50:09Z","timestamp":1788857409000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2296-3529\/13\/3\/42"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,8]]},"references-count":88,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["dermatopathology13030042"],"URL":"https:\/\/doi.org\/10.3390\/dermatopathology13030042","relation":{},"ISSN":["2296-3529"],"issn-type":[{"value":"2296-3529","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,8]]}}}