{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:53:51Z","timestamp":1760241231721,"version":"build-2065373602"},"reference-count":9,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research project SATIN (Sviluppo di Approcci Terapeutici Innovativi per patologie neoplastiche resistenti ai trattamenti)","award":["D.D. n. 355 del 5\/06\/2017 \u2013 Fondo FESR 2014\/2020"],"award-info":[{"award-number":["D.D. n. 355 del 5\/06\/2017 \u2013 Fondo FESR 2014\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Coeliac disease (CD) is a multifactorial autoimmune disorder and gut dysbiosis contributes to its pathogenesis. We previously profiled by 16S rRNA sequencing duodenal and oropharyngeal microbiomes in active CD (a-CD), gluten-free diet (GFD) patients, and controls (CO) and found significantly higher levels of Neisseria spp., with pro-inflammatory activities, in a-CD patients than in the other two groups. In this study, we developed a fast and simple qPCR-based method to evaluate the abundance of the oral Neisseria spp. and the diagnostic performances of the test in CD diagnosis. The Neisseria spp. abundances detected by quantitative PCR (qPCR) were: CO = 0.14, GFD = 0.15, a-CD = 2.08, showing a similar trend to those previously measured by next generation sequencing (NGS). In particular, Neisseria spp. values obtained by both methods were significantly higher (p &lt; 0.001) in a-CD than in the other two groups GFD and CO\u2014the latter almost overlapping. We calculated by ROC curve analysis the threshold of 1.12 ng\/\u03bcL of Neisseria spp. to discriminate between CO+GFD and a-CD patients with 100% and 96.7% of diagnostic sensitivity and specificity, respectively. In conclusion, our data, if confirmed in other cohorts, suggest the q-PCR evaluation of oral Neisseria spp. could be a fast and simple method to assess CD-associated dysbiosis for diagnostic purposes.<\/jats:p>","DOI":"10.3390\/diagnostics10010012","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T05:37:08Z","timestamp":1577425028000},"page":"12","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Setup of Quantitative PCR for Oral Neisseria spp. Evaluation in Celiac Disease Diagnosis"],"prefix":"10.3390","volume":"10","author":[{"given":"Maria Valeria","family":"Esposito","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, 80131 Naples, Italy"},{"name":"Ceinge Biotecnologie Avanzate S. C. a R. L., 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9540-3405","authenticated-orcid":false,"given":"Carmela","family":"Nardelli","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, 80131 Naples, Italy"},{"name":"Ceinge Biotecnologie Avanzate S. C. a R. L., 80131 Naples, Italy"},{"name":"Task Force on Microbiome Studies, University of Naples Federico II, 80100 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"Granata","sequence":"additional","affiliation":[{"name":"LabGTP (Laboratory of Genomics, Transcriptomics and Proteomics), Institute for High Performance Computing and Networking (ICAR), National Research Council (CNR), 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Pagliuca","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9273-3698","authenticated-orcid":false,"given":"Valeria","family":"D\u2019Argenio","sequence":"additional","affiliation":[{"name":"Ceinge Biotecnologie Avanzate S. C. a R. L., 80131 Naples, Italy"},{"name":"Task Force on Microbiome Studies, University of Naples Federico II, 80100 Naples, Italy"},{"name":"San Raffaele Open University, 00166 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Salerno, 84084 Salerno, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2870-8134","authenticated-orcid":false,"given":"Mario Rosario","family":"Guarracino","sequence":"additional","affiliation":[{"name":"LabGTP (Laboratory of Genomics, Transcriptomics and Proteomics), Institute for High Performance Computing and Networking (ICAR), National Research Council (CNR), 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Salvatore","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, 80131 Naples, Italy"},{"name":"Ceinge Biotecnologie Avanzate S. C. a R. L., 80131 Naples, Italy"},{"name":"Task Force on Microbiome Studies, University of Naples Federico II, 80100 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6327-563X","authenticated-orcid":false,"given":"Giovanna","family":"Del Vecchio Blanco","sequence":"additional","affiliation":[{"name":"Department of System Medicine, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7426-1145","authenticated-orcid":false,"given":"Carolina","family":"Ciacci","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Salerno, 84084 Salerno, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Sacchetti","sequence":"additional","affiliation":[{"name":"Ceinge Biotecnologie Avanzate S. C. a R. L., 80131 Naples, Italy"},{"name":"Task Force on Microbiome Studies, University of Naples Federico II, 80100 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/S0140-6736(17)31796-8","article-title":"Coeliac disease","volume":"6","author":"Lebwohl","year":"2018","journal-title":"Lancet"},{"doi-asserted-by":"crossref","unstructured":"Sacchetti, L., and Nardelli, C. (2019). Gut microbiome investigation in celiac disease: From methods to its pathogenetic role. Clin. Chem. Lab. Med.","key":"ref_2","DOI":"10.1515\/cclm-2019-0657"},{"doi-asserted-by":"crossref","unstructured":"Valitutti, F., Cucchiara, S., and Fasano, A. (2019). Celiac Disease and the Microbiome. 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