{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T11:35:01Z","timestamp":1774611301105,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007707","name":"Istituto Nazionale per l'Assicurazione Contro Gli Infortuni sul Lavoro","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100007707","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009888","name":"Regione Toscana","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100009888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Asbestos fibers include a group of silicate minerals that occur in the environment and are widely employed in occupational settings. Asbestos exposure has been associated to various chronic diseases; such as pulmonary fibrosis; mesothelioma; and lung cancer; often characterized by a long period of latency. Underlying mechanisms that are behind the carcinogenic effect of asbestos have not been fully clarified. Therefore; we have conducted an epidemiological study to evaluate the relationship between 8-hydroxy-2\u2032-deoxyguanosine (8-oxodG), one of the most reliable biomarkers of oxidative stress and oxidative DNA damage; and asbestos exposure in the peripheral blood of residents in Tuscany and Liguria regions; Italy; stratified by occupational exposure to this carcinogen. Levels of 8-oxodG were expressed such as relative adduct labeling (RAL); the frequency of 8-oxodG per 105 deoxyguanosine was significantly higher among exposed workers with respect to the controls; i.e., 3.0 \u00b1 0.2 Standard Error (SE) in asbestos workers versus a value of 1.3 \u00b1 0.1 (SE) in unexposed controls (p &lt; 0.001). When the relationship with occupational history was investigated; significant higher levels of 8-oxodG were measured in current and former asbestos workers vs. healthy controls; 3.1 \u00b1 0.3 (SE) and 2.9 \u00b1 0.2 (SE), respectively. After stratification for occupational history; a significant 194% excess of adducts was found in workers with 10 or more years of past asbestos exposure (p &lt; 0.001). 8-oxodG can be used for medical surveillance programs of cohorts of workers with past and recent exposures to carcinogens for the identification of subjects requiring a more intense clinical surveillance.<\/jats:p>","DOI":"10.3390\/diagnostics10040239","type":"journal-article","created":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T04:15:13Z","timestamp":1587528913000},"page":"239","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Chromatographic Detection of 8-Hydroxy-2\u2032-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures"],"prefix":"10.3390","volume":"10","author":[{"given":"Filippo","family":"Cellai","sequence":"first","affiliation":[{"name":"Cancer Risk Factor Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3833-6717","authenticated-orcid":false,"given":"Stefano","family":"Bonassi","sequence":"additional","affiliation":[{"name":"Clinical and Molecular Epidemiology, IRCCS San Raffaele Pisana, 00166 Rome, Italy"},{"name":"Department of Human Sciences and Quality of Life Promotion, San Raffaele University, 00166 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfonso","family":"Cristaudo","sequence":"additional","affiliation":[{"name":"Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56010 Pisa, Italy"},{"name":"Occupational Medicine Unit, University of Pisa, 56010 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Bonotti","sequence":"additional","affiliation":[{"name":"Occupational Medicine Unit, University of Pisa, 56010 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4796-3675","authenticated-orcid":false,"given":"Monica","family":"Neri","sequence":"additional","affiliation":[{"name":"Clinical and Molecular Epidemiology, IRCCS San Raffaele Pisana, 00166 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcello","family":"Ceppi","sequence":"additional","affiliation":[{"name":"Unit of Clinical Epidemiology, IRCCS Ospedale Policlinico San Martino, 16131 Genoa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8821-4542","authenticated-orcid":false,"given":"Marco","family":"Bruzzone","sequence":"additional","affiliation":[{"name":"Unit of Clinical Epidemiology, IRCCS Ospedale Policlinico San Martino, 16131 Genoa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9837-7185","authenticated-orcid":false,"given":"Mirta","family":"Mili\u0107","sequence":"additional","affiliation":[{"name":"Mutagenesis Unit, Institute for Medical Research and Occupational Health, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armelle","family":"Munnia","sequence":"additional","affiliation":[{"name":"Cancer Risk Factor Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7646-7967","authenticated-orcid":false,"given":"Marco","family":"Peluso","sequence":"additional","affiliation":[{"name":"Cancer Risk Factor Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,21]]},"reference":[{"key":"ref_1","first-page":"1067","article-title":"Study on genotoxicity, oxidative stress biomarkers and clinical symptoms in workers of an asbestos-cement factory","volume":"14","author":"Afaghi","year":"2015","journal-title":"Excli. J."},{"key":"ref_2","first-page":"293","article-title":"Mortality from lung cancer and other causes among workers in an asbestos textile factory","volume":"25","author":"Knox","year":"1968","journal-title":"Br. J. Ind. Med."},{"key":"ref_3","unstructured":"IARC (1987). Overall evaluations of carcinogenicity: An updating of IARC Monographs volumes 1 to 42. IARC Monogr. Eval. Carcinog. Risk Chem. Hum. Suppl., 7, 1\u2013440."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1080\/10937404.2011.556045","article-title":"Applying definitions of \u201casbestos\u201d to environmental and \u201clow-dose\u201d exposure levels and health effects, particularly malignant mesothelioma","volume":"14","author":"Case","year":"2011","journal-title":"J. Toxicol. Environ. Health Part B"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1080\/10937404.2011.556047","article-title":"Pulmonary endpoints (lung carcinomas and asbestosis) following inhalation exposure to asbestos","volume":"14","author":"Mossman","year":"2011","journal-title":"J. Toxicol. Environ. Health Part B"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.freeradbiomed.2018.10.401","article-title":"Iron addiction with ferroptosis-resistance in asbestos-induced mesothelial carcinogenesis: Toward the era of mesothelioma prevention","volume":"133","author":"Toyokuni","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1021\/tx700404a","article-title":"The pattern of p53 mutations caused by PAH o-quinones is driven by 8-oxo-dGuo formation while the spectrum of mutations is determined by biological selection for dominance","volume":"21","author":"Park","year":"2008","journal-title":"Chem. Res. Toxicol."},{"key":"ref_8","first-page":"99","article-title":"Distinct Spectrum of Mutations Induced by Crocidolite Asbestos","volume":"62","author":"Unfried","year":"2002","journal-title":"Cancer Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1042\/BST0361071","article-title":"Biomarkers of oxidative damage to DNA and repair","volume":"36","author":"Loft","year":"2008","journal-title":"Biochem. Soc. Trans."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1080\/10590500902885684","article-title":"8-hydroxy-2\u2032 -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis","volume":"27","author":"Valavanidis","year":"2009","journal-title":"J. Environ. Sci. Health. Part CEnviron. Carcinog. Ecotoxicol. Rev."},{"key":"ref_11","first-page":"395","article-title":"8-Oxo-7, 8-dihydro-2-deoxyguanosine and other lesions along the coding strand of the exon 5 of the tumour suppressor gene P53 in a breast cancer case-control study","volume":"23","author":"Brancato","year":"2016","journal-title":"DNA Res. Int. J. Rapid Publ. Rep. Genes Genomes"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1016\/S0891-5849(03)00060-1","article-title":"Multiple roles of oxidants in the pathogenesis of asbestos-induced diseases","volume":"34","author":"Shukla","year":"2003","journal-title":"Free Radic. Biol. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1080\/10937404.2011.556051","article-title":"Role of mutagenicity in asbestos fiber-induced carcinogenicity and other diseases","volume":"14","author":"Huang","year":"2011","journal-title":"J. Toxicol. Environ. Health Part B Crit. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.mrgentox.2010.12.014","article-title":"Exposure to asbestos: Correlation between blood levels of mesothelin and frequency of micronuclei in peripheral blood lymphocytes","volume":"721","author":"Marini","year":"2011","journal-title":"Mutat Res."},{"key":"ref_15","first-page":"232","article-title":"Association between polymorphisms of DNA repair gene XRCC1 and DNA damage in asbestos-exposed workers","volume":"19","author":"Zhao","year":"2006","journal-title":"Biomed. Environ. Sci. BES"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"598","DOI":"10.4238\/2014.January.28.5","article-title":"Elevated micronucleus frequencies in patients with pleural plaque secondary to environmental exposure to asbestos","volume":"13","author":"Govercin","year":"2014","journal-title":"Genet. Mol. Res. GMR"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S1383-5718(00)00053-X","article-title":"Levels of 8-hydroxy-2\u2032-deoxyguanosine in DNA of white blood cells from workers highly exposed to asbestos in Germany","volume":"468","author":"Marczynski","year":"2000","journal-title":"Mutat. Res."},{"key":"ref_18","first-page":"357","article-title":"Increased urinary excretion of the oxidative DNA adduct, 8-hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azo-dye industries","volume":"6","author":"Tagesson","year":"1993","journal-title":"Pol. J. Occup. Med. Environ. Health"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"186","DOI":"10.2486\/indhealth.39.186","article-title":"Urinary 8-oxo-7, 8-dihydro-2\u2032-deoxyguanosine and biopyrrins levels among construction workers with asbestos exposure history","volume":"39","author":"Yoshida","year":"2001","journal-title":"Ind. Health"},{"key":"ref_20","first-page":"15","article-title":"Helsinki Criteria update 2014: Asbestos continues to be a challenge for disease prevention and attribution","volume":"40","author":"Vainio","year":"2016","journal-title":"Epidemiol. Prev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.canlet.2017.06.028","article-title":"Germline mutations in DNA repair genes predispose asbestos-exposed patients to malignant pleural mesothelioma","volume":"405","author":"Betti","year":"2017","journal-title":"Cancer Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1136\/oemed-2016-104119","article-title":"The epidemiology of malignant mesothelioma in women: Gender differences and modalities of asbestos exposure","volume":"75","author":"Marinaccio","year":"2018","journal-title":"Occup. Environ. Med."},{"key":"ref_23","unstructured":"Mohamadkhani, A., Pourshams, A., Viti, J., Cellai, F., Mortazavi, K., Sharafkhah, M., Sotoudeh, M., MalekZadeh, R., Boffetta, P., and Peluso, M. (2017). Pancreatic cancer patients are prone to the higher oxidative DNA damage of peripheral leukocytes. Asian. Pac. J. Cancer Prev."},{"key":"ref_24","first-page":"385","article-title":"Methodology and results of an experience of medical surveillance of people previously exposed to asbestos in Tuscany","volume":"32","author":"Cristaudo","year":"2010","journal-title":"G. Ital. di Med. del Lav. ed Ergon."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.toxlet.2015.09.026","article-title":"The oxidation of p-phenylenediamine, an ingredient used for permanent hair dyeing purposes, leads to the formation of hydroxyl radicals: Oxidative stress and DNA damage in human immortalized keratinocytes","volume":"239","author":"Zanoni","year":"2015","journal-title":"Toxicol. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1093\/toxsci\/kfx246","article-title":"Paternal Exposure to Environmental Chemical Stress Affects Male Offspringns Hepatic Mitochondria","volume":"162","author":"Godschalk","year":"2018","journal-title":"Toxicol. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1093\/mutage\/gem030","article-title":"32P-Post-labelling method improvements for aromatic compound-related molecular epidemiology studies","volume":"22","author":"Munnia","year":"2007","journal-title":"Mutagenesis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1002\/sim.1040","article-title":"Advanced methods in meta-analysis: Multivariate approach and meta-regression","volume":"21","author":"Arends","year":"2002","journal-title":"Stat. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1093\/carcin\/bgl208","article-title":"Interplay between Helicobacter pylori and host gene polymorphisms in inducing oxidative DNA damage in the gastric mucosa","volume":"28","author":"Izzotti","year":"2007","journal-title":"Carcinogenesis"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1080\/15376510309830","article-title":"Methodology for evaluating oxidative DNA damage and metabolic genotypes in human trabecular meshwork","volume":"13","author":"Izzotti","year":"2003","journal-title":"Toxicol. Mech. Methods"},{"key":"ref_31","first-page":"2472","article-title":"Modulation of biomarkers by chemopreventive agents in smoke-exposed rats","volume":"61","author":"Izzotti","year":"2001","journal-title":"Cancer Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.1093\/carcin\/bgu154","article-title":"Assay of lapatinib in murine models of cigarette smoke carcinogenesis","volume":"35","author":"Balansky","year":"2014","journal-title":"Carcinogenesis"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1007\/s00204-012-0993-1","article-title":"Oxidative stress in the lung of mice exposed to cigarette smoke either early in life or in adulthood","volume":"87","author":"Micale","year":"2013","journal-title":"Arch. Toxicol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1093\/carcin\/17.5.917","article-title":"Sensitive detection of 8-hydroxy-2\u2032deoxyguanosine in DNA by 32P-postlabeling assay and the basal levels in rat tissues","volume":"17","author":"Devanaboyina","year":"1996","journal-title":"Carcinogenesis"},{"key":"ref_35","unstructured":"IARC (2012). Biological agents. Volume 100 B. A review of human carcinogens. Iarc. Monogr. Eval. Carcinog. Risks Hum., 100, 1\u2013441."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.mrrev.2003.06.010","article-title":"Linking exposure to environmental pollutants with biological effects","volume":"544","author":"Sorensen","year":"2003","journal-title":"Mutat. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxlet.2017.02.008","article-title":"3-(2-deoxy-\u03b2-d-erythro-pentafuranosyl) pyrimido [1, 2-\u03b1] purin-10 (3H)-one deoxyguanosine adducts of workers exposed to asbestos fibers","volume":"270","author":"Bonassi","year":"2017","journal-title":"Toxic. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/jes.2012.30","article-title":"Personal exposure to volatile organic compounds in the Czech Republic","volume":"22","author":"Svecova","year":"2012","journal-title":"J. Expo. Sci. Environ. Epidemiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1093\/mutage\/gev014","article-title":"Oxidatively damaged DNA in the nasal epithelium of workers occupationally exposed to silica dust in Tuscany region, Italy","volume":"30","author":"Peluso","year":"2015","journal-title":"Mutagenesis"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.mrfmmm.2009.04.008","article-title":"The impact of air pollution on the levels of micronuclei measured by automated image analysis","volume":"669","author":"Rossnerova","year":"2009","journal-title":"Mutat. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.mrfmmm.2007.02.032","article-title":"Biomarkers of air pollution exposure\u2014A study of policemen in Prague","volume":"624","author":"Topinka","year":"2007","journal-title":"Mutat. Res."},{"key":"ref_42","first-page":"1","article-title":"Mechanisms of asbestos-induced carcinogenesis","volume":"71","author":"Toyokuni","year":"2009","journal-title":"Nagoya. J. Med. Sci."},{"key":"ref_43","first-page":"160","article-title":"Linking the generation of DNA adducts to lung cancer","volume":"390","author":"Ceppi","year":"2017","journal-title":"Toxic In Vitro"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Mili\u0107, M., Neri, M., Ceppi, M., Bruzzone, M., Munnia, A., Ugolini, D., Cristaudo, A., Bonotti, A., Peluso, M.E., and Bonassi, S. (2018). DNA damage and genomic instability among workers formerly and currently exposed to asbestos. Scand. J. Work Environ. Health.","DOI":"10.5271\/sjweh.3721"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1080\/01926230490885733","article-title":"Ferruginous bodies: Implications in the mechanism of fiber and particle toxicity","volume":"32","author":"Ghio","year":"2004","journal-title":"Toxicol. Pathol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"154","DOI":"10.3109\/10408444.2012.756454","article-title":"Health risk of chrysotile revisited","volume":"43","author":"Bernstein","year":"2013","journal-title":"Crit. Rev. Toxicol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.freeradbiomed.2014.04.004","article-title":"AKT\/mTOR and c-Jun N-terminal kinase signaling pathways are required for chrysotile asbestos-induced autophagy","volume":"72","author":"Lin","year":"2014","journal-title":"Free Radic. Biol. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s12864-015-2354-y","article-title":"Improved transcription and translation with L-leucine stimulation of mTORC1 in Roberts syndrome","volume":"17","author":"Xu","year":"2016","journal-title":"BMC Genom."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3626","DOI":"10.3892\/mmr.2014.3119","article-title":"The c-Jun N-terminal kinase signaling pathway mediates chrysotile asbestos-induced alveolar epithelial cell apoptosis","volume":"11","author":"Li","year":"2015","journal-title":"Mol. Med. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1093\/mutage\/get005","article-title":"Malondialdehyde-deoxyguanosine and bulky DNA adducts in schoolchildren resident in the proximity of the Sarroch industrial estate on Sardinia Island, Italy","volume":"28","author":"Peluso","year":"2013","journal-title":"Mutagenesis"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"303181","DOI":"10.1155\/2013\/303181","article-title":"Age-dependent accumulation of 8-oxoguanine in the DNA and RNA in various rat tissues","volume":"2013","author":"Nie","year":"2013","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2975","DOI":"10.1158\/1055-9965.EPI-04-0906","article-title":"Active and Passive Smoking and Lifestyle Determinants of 8-Oxo-7,8-Dihydro-2\u00e2\u20ac\u00b2-Deoxyguanosine Levels in Human Leukocyte DNA","volume":"14","author":"Lodovici","year":"2005","journal-title":"Cancer Epidemiol. Prev. Biomark."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00420-006-0106-7","article-title":"8-Hydroxy-2-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures","volume":"80","author":"Pilger","year":"2006","journal-title":"Int. Arch. Occup. Environ. Health"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1093\/carcin\/bgg144","article-title":"Oxidative DNA damage and defence gene expression in the mouse lung after short-term exposure to diesel exhaust particles by inhalation","volume":"24","author":"Risom","year":"2003","journal-title":"Carcinogenesis"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.mad.2013.02.008","article-title":"Markers of oxidant stress that are clinically relevant in aging and age-related disease","volume":"134","author":"Jacob","year":"2013","journal-title":"Mech. Ageing Dev."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1002\/em.21693","article-title":"Neighborhood socioeconomic status modifies the association between individual smoking status and PAH-DNA adduct levels in prostate tissue","volume":"53","author":"Rundle","year":"2012","journal-title":"Environ. Mol. Mutagenesis"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Korkmaz, K.S., Butuner, B.D., and Roggenbuck, D. (2018). Detection of 8-OHdG as a diagnostic biomarker. J. Lab. Precis. Med., 3.","DOI":"10.21037\/jlpm.2018.11.01"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ijheh.2015.08.001","article-title":"Biological monitoring of Italian soldiers deployed in Iraq. Results of the SIGNUM project","volume":"219","author":"Bolognesi","year":"2016","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1093\/carcin\/bgz158","article-title":"Modulation of smoke-induced DNA and microRNA alterations in mouse lung by licofelone, a triple COX-1, COX-2 and 5-LOX inhibitor","volume":"41","author":"Izzotti","year":"2020","journal-title":"Carcinogenesis"},{"key":"ref_60","first-page":"1531","article-title":"Modulation by aspirin and naproxen of nucleotide alterations and tumors in the lung of mice exposed to environmental cigarette smoke since birth","volume":"36","author":"Izzotti","year":"2015","journal-title":"Carcinogenesis"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1093\/ije\/dys129","article-title":"DNA methylation differences in exposed workers and nearby residents of the Ma Ta Phut industrial estate, Rayong, Thailand","volume":"41","author":"Peluso","year":"2012","journal-title":"Int. J. Epidemiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1093\/toxsci\/kft241","article-title":"Aberrant methylation of hypermethylated-in-cancer-1 and exocyclic DNA adducts in tobacco smokers","volume":"137","author":"Peluso","year":"2014","journal-title":"Toxicol. Sci. Off. J. Soc. Toxicol."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/4\/239\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:21:35Z","timestamp":1760361695000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/4\/239"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,21]]},"references-count":62,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["diagnostics10040239"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics10040239","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,21]]}}}