{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:41:53Z","timestamp":1785775313537,"version":"3.56.0"},"reference-count":41,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>This study aimed to analyse vitamin D-binding protein (Gc-globulin) serum levels in acute liver failure (ALF) in children in relation to disease outcomes and correlations with other known markers used to evaluate the severity of ALF. Our study included 34 children (mean age 4.87 \u00b1 5.30 years) with ALF of different causes (metabolic, 26.47%; autoimmune, 23.53%; toxic, 20.59%; infection, 17.65%; unknown, 11.76%) and 30 children without any liver injury (mean age 6.11 \u00b1 4.26 years). The outcome was poor in 14 patients (41.18%), including one child with liver transplantation (2.94%). Serum Gc-globulin levels were significantly lower in ALF patients compared to the control group (151.57 \u00b1 171.8 mg\/L vs. 498.63 \u00b1 252.50 mg\/L; p &lt; 0.000001), with an optimum cut-off of 163.5 mg\/L (Area Under the Curve, AUC, 0.8921; sensitivity, 76.50%; specificity, 100%). Levels were also lower in patients with poor outcomes compared to survivors (59.34 \u00b1 33.73 mg\/L vs. 216.12 \u00b1 199.69 mg\/L; p &lt; 0.0001), with an optimum cut-off 115 mg\/L (AUC, 0.7642; sensitivity, 100%; specificity, 50%). Gc-globulin serum levels were variable according to ALF aetiology, i.e., lower in metabolic, infectious, or unknown causes compared to autoimmune and toxic causes. Gc-globulin serum levels were decreased in children with ALF and lower in those with poor outcomes compared with survivors. Gc-globulin serum levels were correlated with other known parameters used to evaluate the severity of ALF and could help to identify patients at high risk for poor outcomes.<\/jats:p>","DOI":"10.3390\/diagnostics10050278","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T14:00:43Z","timestamp":1588600843000},"page":"278","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Vitamin D-Binding Protein (Gc-Globulin) in Acute Liver Failure in Children"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7022-6345","authenticated-orcid":false,"given":"Alina","family":"Grama","sequence":"first","affiliation":[{"name":"2nd Paediatric Clinic, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, 400177 Cluj-Napoca, Romania"},{"name":"Centre of Expertise in Paediatric Liver Rare Diseases, 2nd Paediatric Clinic, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania"},{"name":"University of Medicine and Pharmacy \u201cCarol Davila\u201d, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Burac","sequence":"additional","affiliation":[{"name":"2nd Paediatric Clinic, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, 400177 Cluj-Napoca, Romania"},{"name":"Centre of Expertise in Paediatric Liver Rare Diseases, 2nd Paediatric Clinic, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornel Olimpiu","family":"Aldea","sequence":"additional","affiliation":[{"name":"Paediatric Nephrology, Dialysis and Toxicology Clinic, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan","family":"Bulata","sequence":"additional","affiliation":[{"name":"Paediatric Nephrology, Dialysis and Toxicology Clinic, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Delean","sequence":"additional","affiliation":[{"name":"Paediatric Nephrology, Dialysis and Toxicology Clinic, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7645-0716","authenticated-orcid":false,"given":"Gabriel","family":"Samasca","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Analysis, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Abrudan","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Analysis, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Sirbe","sequence":"additional","affiliation":[{"name":"2nd Paediatric Clinic, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, 400177 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4931-1219","authenticated-orcid":false,"given":"Tudor Lucian","family":"Pop","sequence":"additional","affiliation":[{"name":"2nd Paediatric Clinic, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, 400177 Cluj-Napoca, Romania"},{"name":"Centre of Expertise in Paediatric Liver Rare Diseases, 2nd Paediatric Clinic, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,4]]},"reference":[{"key":"ref_1","unstructured":"Deirdre, K. (2008). Fulminant hepatitis and acute liver failure. Diseases of the Liver and Biliary System in Children, Wiley-Blackwell. [3rd ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.clinre.2011.03.005","article-title":"Acute liver failure in children","volume":"35","author":"Devictor","year":"2011","journal-title":"Clin. Res. Hepatol. Gastroenterol."},{"key":"ref_3","unstructured":"Mauss, S., Berg, T., Sarazzin, J., and Wedemeyer, H. (2017). Acute liver failure. Hepatology\u2014A Clinical Textbook, Flying Publisher. [6th ed.]."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1136\/gut.17.7.489","article-title":"Factor VII levels as a guide to prognosis in fulminant hepatic failure","volume":"17","author":"Gazzard","year":"1976","journal-title":"Gut"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1002\/hep.510230630","article-title":"Coagulation factor V levels as a prognostic indicator in fulminant hepatic failure","volume":"23","author":"Izumi","year":"1996","journal-title":"Hepatology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1136\/adc.2006.115113","article-title":"Prognostic factors in paediatric acute liver failure","volume":"93","author":"Ciocca","year":"2008","journal-title":"Arch. Dis. Child."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.cld.2008.10.001","article-title":"Coagulopathy of acute liver failure","volume":"13","author":"Munoz","year":"2009","journal-title":"Clin. Liver Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/j.1365-2141.1975.tb01836.x","article-title":"Coagulation studies as a prognostic index in acute liver failure","volume":"29","author":"Dymock","year":"1975","journal-title":"Br. J. Haematol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1002\/lt.21104","article-title":"MELD is superior to King\u2019s College and Clichy\u2019s criteria to assess prognosis in fulminant hepatic failure","volume":"13","author":"Yantorno","year":"2007","journal-title":"Liver Transpl."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"450","DOI":"10.5152\/tjg.2016.16431","article-title":"Etiologies, outcomes, and prognostic factors of pediatric acute liver failure: A single center\u2019s experience in Turkey","volume":"27","author":"Canan","year":"2016","journal-title":"Turk. J. Gastroenterol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1055\/s-2008-1073115","article-title":"Acute Liver Failure in Children","volume":"28","author":"Squires","year":"2008","journal-title":"Semin. Liver Dis."},{"key":"ref_12","first-page":"61","article-title":"Aetiology, outcomes and prognostic indicators of paediatric acute liver failure","volume":"88","author":"Gilbert","year":"2018","journal-title":"An. Pediatr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0016-5085(89)90081-4","article-title":"Early indicators of prognosis in fulminant hepatic failure","volume":"97","author":"Alexander","year":"1989","journal-title":"Gastroenterology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1053\/jhep.2001.22172","article-title":"A model to predict survival in patients with end-stage liver disease","volume":"33","author":"Kamath","year":"2001","journal-title":"Hepatology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.cgh.2015.10.007","article-title":"Ability of King\u2019s College Criteria and Model for End-Stage Liver Disease Scores to Predict Mortality of Patients with Acute Liver Failure: A Meta-analysis","volume":"14","author":"McPhail","year":"2016","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1002\/lt.20437","article-title":"Gc-globulin and prognosis in acute liver failure","volume":"11","author":"Rossaro","year":"2005","journal-title":"Liver Transpl."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1002\/hep.1840050220","article-title":"Decreased serum group-specific component protein levels and complexes with actin in fulminant hepatic necrosis","volume":"5","author":"Lee","year":"1985","journal-title":"Hepatology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1016\/0016-5085(88)90686-5","article-title":"Proportion of circulating Gc (vitamin D-binding protein) in complexed form: Relation to clinical outcome in fulminant hepatic necrosis","volume":"94","author":"Lee","year":"1988","journal-title":"Gastroenterology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0009-8981(85)90144-5","article-title":"Accurate quantitation of native Gc protein in serum and estimation of endogenous Gc: G-actin complexes by rocket immunoelectrophoresis","volume":"148","author":"Galbraith","year":"1985","journal-title":"Clin. Chim. Acta"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1309\/LM749BQETKYPYPVM","article-title":"Pre-analytical Variables in Coagulation Testing Associated with Diagnostic Errors in Haemostasis","volume":"43","author":"Favaloro","year":"2012","journal-title":"Lab. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"213","DOI":"10.32614\/RJ-2016-042","article-title":"easyROC: An Interactive Web-tool for ROC Curve Analysis Using R Language Environment","volume":"8","author":"Goksuluk","year":"2016","journal-title":"R J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0001-2998(78)80014-2","article-title":"Basic principles of ROC analysis","volume":"8","author":"Metz","year":"1978","journal-title":"Semin. Nucl. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1097\/PEC.0000000000000884","article-title":"Acute Liver Failure: Outcome and Value of Pediatric End-Stage Liver Disease Score in Pediatric Cases","volume":"34","author":"Montoro","year":"2018","journal-title":"Pediatr. Emerg. Care"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1097\/MPG.0b013e3182349a04","article-title":"Pediatric end-stage liver disease score in acute liver failure to assess poor prognosis","volume":"54","author":"Sanchez","year":"2012","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_25","first-page":"101","article-title":"Prognostic factors in liver failure in children by discriminant analysis of clinical data. A chemometric approach","volume":"60","author":"Pop","year":"2015","journal-title":"Studia UBB Chemia"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"S304","DOI":"10.1016\/S0168-8278(16)00397-4","article-title":"PELD score as a prognostic factor in fulminant liver failure caused by mushroom poisoning in children","volume":"64","author":"Pop","year":"2016","journal-title":"J. Hepatol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"s417","DOI":"10.1016\/S0168-8278(13)61014-4","article-title":"Serum creatinine and the presence of encephalopathy at presentation may predict mortality in children with acute liver failure","volume":"58","author":"Stefanescu","year":"2013","journal-title":"J. Hepatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1056\/NEJMra1208937","article-title":"Acute liver failure","volume":"369","author":"Bernal","year":"2013","journal-title":"N. Engl. J. Med."},{"key":"ref_29","unstructured":"Hay, W., Myron, L., Sondheimer, J.M., and Deterding, R.R. (2011). Liver and pancreas. Current Diagnosis & Treatment Pediatrics, Mc Graw Hill Education. [20th ed.]."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Grama, A., Aldea, C., Burac, L., Delean, D., Boghitoiu, D., Bulata, B., Nitescu, V., Ulmeanu, C., and Pop, T.L. (2019). Acute liver failure secondary to toxic exposure in children. Arch. Med. Sci.","DOI":"10.5114\/aoms.2019.87716"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"30","DOI":"10.26416\/Pedi.53.1.2019.2349","article-title":"Treatment of acute liver failure in children","volume":"53","author":"Grama","year":"2019","journal-title":"Pediatru.ro"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Grama, A., Blaga, L., Nicolescu, A., Deleanu, C., Militaru, M., Cainap, S.S., Pop, I., Tita, G., Sirbe, C., and Fufezan, O. (2019). Novel Mutation in GALT Gene in Galactosemia Patient with Group B Streptococcus Meningitis and Acute Liver Failure. Medicina, 55.","DOI":"10.3390\/medicina55040091"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1111\/j.1699-0463.1959.tb04844.x","article-title":"Immune-electrophoretic demonstration of qualitative differences in human sera and their relation to the haptoglobins","volume":"47","author":"Hirschfeld","year":"1959","journal-title":"Acta Pathol. Microbiol. Scand."},{"key":"ref_34","first-page":"131","article-title":"Gc-globulin in liver disease","volume":"55","author":"Schiodt","year":"2008","journal-title":"Dan. Med. Bull."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1007\/s001340000837","article-title":"Trauma stimulates the synthesis of Gc-globulin","volume":"27","author":"Dahl","year":"2001","journal-title":"Intensive Care Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/lt.500030104","article-title":"Immunonephelometric quantification of group-specific component protein in patients with acute liver failure","volume":"3","author":"Wians","year":"1997","journal-title":"Liver Transpl. Surg."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"947","DOI":"10.7326\/0003-4819-137-12-200212170-00007","article-title":"Results of a prospective study of acute liver failure at 17 tertiary care centers in the United States","volume":"137","author":"Ostapowicz","year":"2002","journal-title":"Ann. Intern. Med."},{"key":"ref_38","first-page":"635","article-title":"Serial measurements of serum Gc-globulin in acetaminophen intoxication","volume":"7","author":"Bondesen","year":"1995","journal-title":"Eur. J. Gastroenterol. Hepatol."},{"key":"ref_39","first-page":"713","article-title":"Admission levels of serum Gc-globulin: Predictive value in fulminant hepatic failure","volume":"23","author":"Bondesen","year":"1996","journal-title":"Hepatology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1053\/jlts.2001.26061","article-title":"Temporal profile of total, bound, and free Gc-globulin after acetaminophen overdose","volume":"7","author":"Ott","year":"2001","journal-title":"Liver Transpl."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1002\/lt.21196","article-title":"Actin-Free Gc Globulin: A Rapidly Assessed Biomarker of Organ Dysfunction in Acute Liver Failure and Cirrhosis","volume":"13","author":"Antoniades","year":"2007","journal-title":"Liver Transpl."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/5\/278\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:25:18Z","timestamp":1760361918000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/5\/278"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,4]]},"references-count":41,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["diagnostics10050278"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics10050278","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,4]]}}}