{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T13:31:23Z","timestamp":1785159083794,"version":"3.55.0"},"reference-count":22,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Extravillous trophoblast cells (EVTs) secreted by the uterine cavity may help overcome limitations associated with prenatal testing currently in use. EVTs are isolated using a routine safe liquid-based Pap test (called ThinPrep); however, the ThinPrep solution contains alcohol that hinders the isolation of intact EVTs. We compared the trophoblastic cell isolation efficiency of two different methods of fixation: Thinprep (pre-fixation method) and formalin (post-fixation method). We analyzed EVTs from 20 pregnant women (5\u201320 weeks of gestation) who underwent invasive prenatal testing. The percentages of placental \u03b2-human chorionic gonadotropin (\u03b2-hCG)-expressing cells were calculated. The presence of XY chromosomes were used to confirm pure trophoblast cells by fluorescence in situ hybridization. The \u03b2-hCG-positive cells obtained from pre- and post-fixation were 66.4 \u00b1 13.3% and 83.2 \u00b1 8.1% (p = 0.003), respectively, and fluorescence-positive cells were 11.1 \u00b1 2.1% and 23.8 \u00b1 4.8%, respectively (p = 0.001). Post-fixation was found to be more efficient in isolating non-trophoblast cells than pre-fixation. For the successful clinical application of trophoblast retrieval and isolation from the cervix in prenatal genetic testing, each step should be optimized for consistent and reliable results.<\/jats:p>","DOI":"10.3390\/diagnostics10050300","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T10:27:19Z","timestamp":1589452039000},"page":"300","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Optimization Protocol of Fixation Method for Trophoblast Retrieval from the Cervix (TRIC): A Preliminary Study"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0218-8951","authenticated-orcid":false,"given":"Min Jin","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Ilsan Medical Center, CHA University, Gyenoggi-do KS009, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1118-4920","authenticated-orcid":false,"given":"Soo Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University, Seoul KS013, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Han","family":"Shim","sequence":"additional","affiliation":[{"name":"Genetic Laboratory, Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul KS013, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Yeon","family":"Jang","sequence":"additional","affiliation":[{"name":"Genetic Laboratory, Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul KS013, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0538-3517","authenticated-orcid":false,"given":"Hee Jin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University, Seoul KS013, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0722-1714","authenticated-orcid":false,"given":"Dong Hyun","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University, Seoul KS013, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,14]]},"reference":[{"key":"ref_1","unstructured":"(2019, May 29). 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Head Neck Surg."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/5\/300\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:28:44Z","timestamp":1760174924000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/5\/300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,14]]},"references-count":22,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["diagnostics10050300"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics10050300","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,14]]}}}