{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T14:28:35Z","timestamp":1762352915331,"version":"build-2065373602"},"reference-count":64,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,5,31]],"date-time":"2020-05-31T00:00:00Z","timestamp":1590883200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009543","name":"Pontificia Universidad Javeriana","doi-asserted-by":"publisher","award":["5633"],"award-info":[{"award-number":["5633"]}],"id":[{"id":"10.13039\/501100009543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: Mucopolysaccharidoses (MPS) are a group of inherited metabolic diseases caused by impaired function or absence of lysosomal enzymes involved in degradation of glycosaminoglycans. Clinically, MPS are skeletal dysplasias, characterized by cartilage abnormalities and disturbances in the process of endochondral ossification. Histologic abnormalities of growth cartilage have been reported at advanced stages of the disease, but information regarding growth plate pathology progression either in humans or in animal models, as well as its pathophysiology, is limited. Methods: Histological analyses of distal femur growth plates of wild type (WT) and mucopolysaccharidosis type VI (MPS VI) rats at different stages of development were performed, including quantitative data. Experimental findings were then analyzed in a theoretical scenario. Results: Histological evaluation showed a progressive loss of histological architecture within the growth plate. Furthermore, in silico simulation suggest the abnormal cell distribution in the tissue may lead to alterations in biochemical gradients, which may be one of the factors contributing to the growth plate abnormalities observed, highlighting aspects that must be the focus of future experimental works. Conclusion: The results presented shed some light on the progression of growth plate alterations observed in MPS VI and evidence the potentiality of combined theoretical and experimental approaches to better understand pathological scenarios, which is a necessary step to improve the search for novel therapeutic approaches.<\/jats:p>","DOI":"10.3390\/diagnostics10060360","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T11:49:21Z","timestamp":1591012161000},"page":"360","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Growth Plate Pathology in the Mucopolysaccharidosis Type VI Rat Model\u2014An Experimental and Computational Approach"],"prefix":"10.3390","volume":"10","author":[{"given":"Johana M.","family":"Guevara-Morales","sequence":"first","affiliation":[{"name":"Institute for the Study of Inborn Errors of Metabolism, (IEIM), Pontificia Universidad, Javeriana, Bogot\u00e1 4665684, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0167-116X","authenticated-orcid":false,"given":"Michael","family":"Frohbergh","sequence":"additional","affiliation":[{"name":"Exponent Inc., Philadelphia, PA 19104, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector","family":"Castro-Abril","sequence":"additional","affiliation":[{"name":"Numerical Methods and Modeling Research Group (GNUM)-Biomimetics Laboratory, Universidad Nacional de Colombia, Bogot\u00e1 4665684, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan J.","family":"Vaca-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Nefertiti, Wellness and New Technologies Group, School of Health and Sports Sciences, Fundaci\u00f3n Universitaria del \u00c1rea Andina, Bogot\u00e1 4665684, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis A.","family":"Barrera","sequence":"additional","affiliation":[{"name":"Institute for the Study of Inborn Errors of Metabolism, (IEIM), Pontificia Universidad, Javeriana-Clinic for Inborn Errors of Metabolism (CEIM)-Hospital Universitario San Ignacio, Bogot\u00e1 4665684, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego A.","family":"Garz\u00f3n-Alvarado","sequence":"additional","affiliation":[{"name":"Numerical Methods and Modeling Research Group (GNUM)-Biomimetics Laboratory, Universidad Nacional de Colombia, Bogot\u00e1 4665684, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Schuchman","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Calogera","family":"Simonaro","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,31]]},"reference":[{"key":"ref_1","unstructured":"Valle, D., Vogelstain, B., Kinzler, K., Antonarakis, S., Ballabio, A., Mitchell, G.A., Brunetti-Pierri, N., Scriver, C.R., Sly, W.S., and Beaudet, A. 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