{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:50:41Z","timestamp":1790211041246,"version":"4.1.0"},"reference-count":33,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007300","name":"China Medical University","doi-asserted-by":"publisher","award":["DMR-109-085, DMR-109-056, and CMU108-S-38"],"award-info":[{"award-number":["DMR-109-085, DMR-109-056, and CMU108-S-38"]}],"id":[{"id":"10.13039\/501100007300","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Taiwan Ministry of Science and Technology","award":["MOST 107-2314-B-039-036 and MOST 107-2320-B-039-034"],"award-info":[{"award-number":["MOST 107-2314-B-039-036 and MOST 107-2320-B-039-034"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: Interstitial cystitis\/painful bladder syndrome (IC\/PBS) could be treated to ameliorate urothelial injury. Here, we investigated the efficacy of intravesical instillation with platelet-rich plasma (PRP) and hyaluronic acid for acute IC\/PBS. Methods: The effects of PRP and hyaluronic acid on the proliferation of normal human fibroblast cells (HFCs) were assessed. Additionally, thirty virgin female rats were randomized into five groups: group 1, saline-injected control; group 2, cyclophosphamide (CYP) plus intravesical instillation with normal saline; group 3, CYP plus intravesical instillation with hyaluronic acid (1 mg\/mL); group 4, CYP plus intravesical instillation with PRP; and group 5, CYP plus intravesical instillation with PRP plus hyaluronic acid. A cystometry and histological assessments were performed. The expression of cell junction-associated protein zonula occludens-2 (ZO-2) and inflammatory cytokine interleukin 6 (IL-6) was also measured. Results: Low dose PRP increased proliferation in HFCs. The acute IC\/PBS rats showed significantly lower voiding interval values. Voiding interval values were significantly higher in the CYP plus intravesical instillation with PRP group than in the CYP-induced acute IC\/PBS group. Additionally, the expression of ZO-2 was increased and IL-6 was decreased in the CYP plus intravesical instillation with PRP group compared with the CYP-induced acute IC\/PBS group. Conclusion: These findings suggest that PRP modulate urothelial repair, which ameliorate the increase in urination frequency in rats treated with CYP. Overall, PRP may confer potential benefits by acting as urothelial repair modulators.<\/jats:p>","DOI":"10.3390\/diagnostics10060381","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T04:19:39Z","timestamp":1591676379000},"page":"381","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Platelet-Rich Plasma Ameliorates Cyclophosphamide-Induced Acute Interstitial Cystitis\/Painful Bladder Syndrome in a Rat Model"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8756-5113","authenticated-orcid":false,"given":"Yung-Hsiang","family":"Chen","sequence":"first","affiliation":[{"name":"Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan"},{"name":"Departments of Medical Research, Urology and Obstetrics and Gynecology, China Medical University Hospital, Taichung 40447, Taiwan"},{"name":"Department of Psychology, College of Medical and Health Science, Asia University, Taichung 41354, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kee-Ming","family":"Man","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan"},{"name":"Department of Anesthesiology, China Medical University Hsinchu Hospital, Hsinchu 30272, Taiwan"},{"name":"Department of Medicinal Botanicals and Health Applications, Da Yeh University, Changhua 51591, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6333-1638","authenticated-orcid":false,"given":"Wen-Chi","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan"},{"name":"Departments of Medical Research, Urology and Obstetrics and Gynecology, China Medical University Hospital, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2896-1666","authenticated-orcid":false,"given":"Po-Len","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Therapy, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kao-Sung","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Applied Cosmetology, Hungkuang University, Taichung 43302, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Yen","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Tzu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Neurology, Kuang Tien General Hospital, Taichung 43303, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huey-Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan"},{"name":"Departments of Medical Research, Urology and Obstetrics and Gynecology, China Medical University Hospital, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Batista, R., Vinagre, N., Meireles, S., Vinagre, J., Prazeres, H., Leao, R., Maximo, V., and Soares, P. (2020). Biomarkers for Bladder Cancer Diagnosis and Surveillance: A Comprehensive Review. Diagnostics, 10.","DOI":"10.3390\/diagnostics10010039"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Akiyama, Y., Luo, Y., Hanno, P.M., Maeda, D., and Homma, Y. (2020). Interstitial cystitis\/bladder pain syndrome: The evolving landscape, animal models and future perspectives. Int. J. Urol.","DOI":"10.1111\/iju.14229"},{"key":"ref_3","first-page":"S99","article-title":"The efficacy of botulinum toxin A and sacral neuromodulation in the management of interstitial cystitis (IC)\/bladder pain syndrome (BPS), what do we know? ICI-RS 2017 think thank, Bristol","volume":"37","author":"Marcelissen","year":"2018","journal-title":"Neurourol. Urodyn."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1007\/s00404-017-4364-2","article-title":"Etiology, pathophysiology and biomarkers of interstitial cystitis\/painful bladder syndrome","volume":"295","author":"Patnaik","year":"2017","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/luts.12214","article-title":"Advances in intravesical therapy for bladder pain syndrome (BPS)\/interstitial cystitis (IC)","volume":"10","author":"Meng","year":"2018","journal-title":"Low Urin Tract Symptoms"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ejphar.2013.03.008","article-title":"Relevance of the cyclophosphamide-induced cystitis model for pharmacological studies targeting inflammation and pain of the bladder","volume":"707","author":"Auge","year":"2013","journal-title":"Eur. J. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0022-5347(05)67495-2","article-title":"Cyclophosphamide-induced cystitis in freely-moving conscious rats: Behavioral approach to a new model of visceral pain","volume":"164","author":"Boucher","year":"2000","journal-title":"J. Urol."},{"key":"ref_8","first-page":"102","article-title":"Inflammatory mediators and interstitial cystitis","volume":"9","author":"Felsen","year":"1991","journal-title":"Semin. Urol."},{"key":"ref_9","first-page":"477","article-title":"Functional, histological structure and mastocytes alterations in rat urinary bladders following acute and [corrected] chronic cyclophosphamide treatment","volume":"61","author":"Juszczak","year":"2010","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.urology.2008.07.031","article-title":"Multiplex analysis of urinary cytokine levels in rat model of cyclophosphamide-induced cystitis","volume":"73","author":"Smaldone","year":"2009","journal-title":"Urology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S0090-4295(99)80334-3","article-title":"The Interstitial Cystitis Data Base Study: Concepts and preliminary baseline descriptive statistics","volume":"49","author":"Simon","year":"1997","journal-title":"Urology"},{"key":"ref_12","first-page":"638","article-title":"Interstitial cystitis\/bladder pain syndrome and glycosaminoglycans replacement therapy","volume":"4","author":"Cervigni","year":"2015","journal-title":"Transl. Androl. Urol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s11626-008-9116-y","article-title":"Characterization of tight junction proteins in cultured human urothelial cells","volume":"44","author":"Rickard","year":"2008","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"188","DOI":"10.5213\/inj.1632548.274","article-title":"The Early Histological Effects of Intravesical Instillation of Platelet-Rich Plasma in Cystitis Models","volume":"20","author":"Donmez","year":"2016","journal-title":"Int. Neurourol. J."},{"key":"ref_15","first-page":"17","article-title":"The use of platelet-rich plasma to enhance the success of bone grafts around dental implants","volume":"11","author":"Garg","year":"2000","journal-title":"Dent. Implantol. Update"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.joms.2003.12.003","article-title":"Platelet-rich plasma: Evidence to support its use","volume":"62","author":"Marx","year":"2004","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1002\/nau.23898","article-title":"Intravesical injections of platelet-rich plasma is effective and safe in treatment of interstitial cystitis refractory to conventional treatment\u2014A prospective clinical trial","volume":"38","author":"Jhang","year":"2019","journal-title":"Neurourol. Urodyn."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1080\/00365590410015876-1","article-title":"Treatment of interstitial cystitis with Cystistat: A hyaluronic acid product","volume":"39","author":"Kallestrup","year":"2005","journal-title":"Scand. J. Urol. Nephrol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/nau.23284","article-title":"Clinical comparison of intravesical hyaluronic acid and chondroitin sulfate therapies in the treatment of bladder pain syndrome\/interstitial cystitis","volume":"37","author":"Gulpinar","year":"2018","journal-title":"Neurourol. Urodyn."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.tjog.2018.04.006","article-title":"Urinary and psychological outcomes in women with interstitial cystitis\/bladder pain syndrome following hyaluronic acid treatment","volume":"57","author":"Liang","year":"2018","journal-title":"Taiwan J. Obstet. Gynecol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1159\/000447863","article-title":"Systematic Review and Meta-Analysis of Intravesical Hyaluronic Acid and Hyaluronic Acid\/Chondroitin Sulfate Instillation for Interstitial Cystitis\/Painful Bladder Syndrome","volume":"39","author":"Pyo","year":"2016","journal-title":"Cell Physiol. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Keay, S.K., Birder, L.A., and Chai, T.C. (2014). Evidence for bladder urothelial pathophysiology in functional bladder disorders. Biomed. Res. Int., 2014.","DOI":"10.1155\/2014\/865463"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1089\/ten.2006.0042","article-title":"Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres","volume":"13","author":"Nagae","year":"2007","journal-title":"Tissue Eng."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ozyuvali, E., Yildirim, M.E., Yaman, T., Kosem, B., Atli, O., and Cimentepe, E. (2016). Protective Effect of Intravesical Platelet-Rich Plasma on Cyclophosphamide-Induced Hemorrhagic Cystitis. Clin. Investig. Med., 39.","DOI":"10.25011\/cim.v39i6.27514"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.tjog.2020.01.013","article-title":"Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladder","volume":"59","author":"Chen","year":"2020","journal-title":"Taiwan J. Obstet. Gynecol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1002\/nau.22970","article-title":"Bladder telemetry: A new approach to evaluate micturition behavior under physiological and inflammatory conditions","volume":"36","author":"Monjotin","year":"2017","journal-title":"Neurourol. Urodyn."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1002\/nau.23141","article-title":"Effects of low energy shock wave therapy on inflammatory moleculars, bladder pain, and bladder function in a rat cystitis model","volume":"36","author":"Wang","year":"2017","journal-title":"Neurourol. Urodyn."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1002\/nau.21180","article-title":"Functional characterization of a chronic cyclophosphamide-induced overactive bladder model in mice","volume":"30","author":"Boudes","year":"2011","journal-title":"Neurourol. Urodyn."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1046\/j.1600-0854.2003.00156.x","article-title":"The uroepithelium: Not just a passive barrier","volume":"5","author":"Apodaca","year":"2004","journal-title":"Traffic"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"F867","DOI":"10.1152\/ajprenal.2000.278.6.F867","article-title":"Everything you wanted to know about the bladder epithelium but were afraid to ask","volume":"278","author":"Lewis","year":"2000","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1124\/jpet.114.214031","article-title":"Characteristics of TRK-130 (Naltalimide), a novel opioid ligand, as a new therapeutic agent for overactive bladder","volume":"350","author":"Fujimura","year":"2014","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/bju.13039","article-title":"A novel urodynamic model for lower urinary tract assessment in awake rats","volume":"115","author":"Schneider","year":"2015","journal-title":"BJU Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4103\/0974-2077.150734","article-title":"Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author\u2019s Perspective","volume":"7","author":"Dhurat","year":"2014","journal-title":"J. Cutan. Aesthet. Surg."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/6\/381\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:36:41Z","timestamp":1760175401000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/6\/381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,8]]},"references-count":33,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["diagnostics10060381"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics10060381","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,8]]}}}