{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T21:27:23Z","timestamp":1783114043934,"version":"3.54.6"},"reference-count":27,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T00:00:00Z","timestamp":1595289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hellenic Foundation for Research and Innovation (H.F.R.I.)","award":["First Call for H.F.R.I. Research Projects to support Faculty members and Researchers and the procurement of high-cost research equipment grant\u201d"],"award-info":[{"award-number":["First Call for H.F.R.I. Research Projects to support Faculty members and Researchers and the procurement of high-cost research equipment grant\u201d"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Oral squamous cell carcinoma (OSCC) is considered an aggressive malignancy, mainly due to its increased propensity to provide local and distant lymph node metastases. Gross chromosome instability (CI; polysomy\/aneuploidy\/monosomy), combined or not with specific gene alterations, is implicated in the development and progression of solid malignancies, including OSCC. In order to further study the relationship between these genetic alterations and the aggressive biological behavior of OSCCs, we investigated the frequency and impact of chromosome 9 numerical imbalances in these tumors. Fifty (n = 50) formalin-fixed, paraffin-embedded primary OSCC tissue sections were used. Chromogenic in situ hybridization (CISH) was implemented for detecting chromosome 9 (CEN\u2014centromere enumeration) numerical alterations. Concerning the screening process in CISH slides, a novel, real-time reference and calibration grid platform was implemented. Chromosome 9 polysomy was observed in 8\/50 (16%) tissue sections, whereas the rest of them demonstrated a normal, diploid pattern (42\/50; 84%). Chromosome 9 polysomy was associated with the grade of differentiation of the examined tumors (p = 0.036). Chromosome 9 numerical imbalances (polysomy) were observed in sub-groups of OSCCs correlating with a progressive dedifferentiation of the malignant tissues. Concerning the implementation of the proposed grid-based platform as described above on CISH slides, it provides a novel, fast, and accurate screening mapping mechanism for detecting chromosome numerical imbalances.<\/jats:p>","DOI":"10.3390\/diagnostics10070501","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T06:38:55Z","timestamp":1595313535000},"page":"501","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Impact of Chromosome 9 Numerical Imbalances in Oral Squamous Cell Carcinoma: A Pilot Grid-Based Centromere Analysis"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5236-7961","authenticated-orcid":false,"given":"Efthymios","family":"Kyrodimos","sequence":"first","affiliation":[{"name":"1st ENT Department, Hippocration General Hospital, University of Athens, 115 27 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6708-2210","authenticated-orcid":false,"given":"Aristeidis","family":"Chrysovergis","sequence":"additional","affiliation":[{"name":"1st ENT Department, Hippocration General Hospital, University of Athens, 115 27 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Mastronikolis","sequence":"additional","affiliation":[{"name":"ENT Department, Medical School, University of Patras, 265 04 Patras, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelos","family":"Tsiambas","sequence":"additional","affiliation":[{"name":"Department of Cytopathology, 417 Veterans Army Hospital (NIMTS), 115 21 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4663-7838","authenticated-orcid":false,"given":"Christos","family":"Riziotis","sequence":"additional","affiliation":[{"name":"Theoretical and Physical Chemistry Institute, Photonics for Nanoapplications Laboratory, National Hellenic Research Foundation, 11635 Athens, Greece"},{"name":"Defence &amp; Security Research Institute, University of Nicosia, CY-2417 Nicosia, Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Roukas","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, 417 Veterans Army Hospital (NIMTS), 115 21 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Fotiades","sequence":"additional","affiliation":[{"name":"Department of Surgery, 424 General Army Hospital, 564 29 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7623-299X","authenticated-orcid":false,"given":"Chara","family":"Stavraka","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Guy\u2019s and St Thomas National Health System Foundation Trust, London SE1 9RT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasileios","family":"Ragos","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial Surgery, Medical School, University of Ioannina, 455 00 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minas","family":"Paschopoulos","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, School of Health Sciences, University of Ioannina, 455 00 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasileios","family":"Papanikolaou","sequence":"additional","affiliation":[{"name":"1st ENT Department, Hippocration General Hospital, University of Athens, 115 27 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1038\/ng1215","article-title":"Chromosome aberrations in solid tumours","volume":"34","author":"Albertson","year":"2003","journal-title":"Nat. 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Int."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/7\/501\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:50:12Z","timestamp":1760176212000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/10\/7\/501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,21]]},"references-count":27,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["diagnostics10070501"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics10070501","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,21]]}}}