{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T17:37:28Z","timestamp":1793813848427,"version":"4.6.1"},"reference-count":45,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T00:00:00Z","timestamp":1601251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009820","name":"Assistance publique-H\u00f4pitaux de Paris","doi-asserted-by":"publisher","award":["EERA"],"award-info":[{"award-number":["EERA"]}],"id":[{"id":"10.13039\/501100009820","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007241","name":"Universit\u00e9 Paris-Saclay","doi-asserted-by":"publisher","award":["EA7361"],"award-info":[{"award-number":["EA7361"]}],"id":[{"id":"10.13039\/501100007241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-33"],"award-info":[{"award-number":["ANR-10-LABX-33"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-14-JAMR-0002"],"award-info":[{"award-number":["ANR-14-JAMR-0002"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014419","name":"EIT Health","doi-asserted-by":"publisher","award":["BL and AMR Detectool"],"award-info":[{"award-number":["BL and AMR Detectool"]}],"id":[{"id":"10.13039\/100014419","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>We have developed a lateral flow immunoassay (LFIA), named NG-Test CTX-M MULTI (NG-Test), to detect group 1, 2, 8, 9, 25 CTX-M producers from agar plates and from positive blood cultures in less than 15 min. The NG-Test was validated retrospectively on 113 well-characterized enterobacterial isolates, prospectively on 102 consecutively isolated ESBL-producers from the Bic\u00eatre hospital and on 100 consecutive blood cultures positive with a gram-negative bacilli (GNB). The NG-Test was able to detect all CTX-M producers grown on the different agar plates used in clinical microbiology laboratories. No false positive nor negative results were observed. Among the 102 consecutive ESBL isolates, three hyper mucous isolates showed an incorrect migration leading to invalid results (no control band). Using an adapted protocol, the results could be validated. The NG-Test detected 99\/102 ESBLs as being CTX-Ms. Three SHV producers were not detected. Among the 100 positive blood cultures with GNB tested 10\/11 ESBL-producers were detected (8 CTX-M-15, 2 CTX-M-27). One SHV-2-producing-E. cloacae was missed. The NG-Test CTX-M MULTI showed 100% sensitivity and specificity with isolates cultured on agar plates and was able to detect 98% of the ESBL-producers identified in our clinical setting either from colonies or from positive blood cultures.<\/jats:p>","DOI":"10.3390\/diagnostics10100764","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T20:03:02Z","timestamp":1601323382000},"page":"764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["A Lateral Flow Immunoassay for the Rapid Identification of CTX-M-Producing Enterobacterales from Culture Plates and Positive Blood Cultures"],"prefix":"10.3390","volume":"10","author":[{"given":"Sandrine","family":"Bernabeu","sequence":"first","affiliation":[{"name":"Team Resist, UMR1184, School of Medicine of Universit\u00e9 Paris-Saclay\u2014INSERM\u2014CEA, LabEx Lermit, 94276 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayaththiry Caroline","family":"Ratnam","sequence":"additional","affiliation":[{"name":"Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Boutal","sequence":"additional","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Laboratoire d\u2019Etudes et de Recherches en Immunonalyse, Universit\u00e9 Paris-Saclay, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u00efs","family":"Vogel","sequence":"additional","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Laboratoire d\u2019Etudes et de Recherches en Immunonalyse, Universit\u00e9 Paris-Saclay, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Devilliers","sequence":"additional","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Laboratoire d\u2019Etudes et de Recherches en Immunonalyse, Universit\u00e9 Paris-Saclay, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Plaisance","sequence":"additional","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Laboratoire d\u2019Etudes et de Recherches en Immunonalyse, Universit\u00e9 Paris-Saclay, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saoussen","family":"Oueslati","sequence":"additional","affiliation":[{"name":"Team Resist, UMR1184, School of Medicine of Universit\u00e9 Paris-Saclay\u2014INSERM\u2014CEA, LabEx Lermit, 94276 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surbhi","family":"Malhotra-Kumar","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Vaccine &amp; Infectious Disease Institute, University of Antwerp, 2610 Antwerp, Belgium"},{"name":"Members of ESCMID Study Group for Antimicrobial Resistance Surveillance\u2014ESGARS, Headquarter, 4010 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7384","authenticated-orcid":false,"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"Team Resist, UMR1184, School of Medicine of Universit\u00e9 Paris-Saclay\u2014INSERM\u2014CEA, LabEx Lermit, 94276 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Fortineau","sequence":"additional","affiliation":[{"name":"Team Resist, UMR1184, School of Medicine of Universit\u00e9 Paris-Saclay\u2014INSERM\u2014CEA, LabEx Lermit, 94276 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6071-8255","authenticated-orcid":false,"given":"St\u00e9phanie","family":"Simon","sequence":"additional","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Laboratoire d\u2019Etudes et de Recherches en Immunonalyse, Universit\u00e9 Paris-Saclay, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Volland","sequence":"additional","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Laboratoire d\u2019Etudes et de Recherches en Immunonalyse, Universit\u00e9 Paris-Saclay, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9937-9572","authenticated-orcid":false,"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"Team Resist, UMR1184, School of Medicine of Universit\u00e9 Paris-Saclay\u2014INSERM\u2014CEA, LabEx Lermit, 94276 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Members of ESCMID Study Group for Antimicrobial Resistance Surveillance\u2014ESGARS, Headquarter, 4010 Basel, Switzerland"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Service de Bact\u00e9riologie, AP-HP, CHU de Bic\u00eatre, 78 Rue du G\u00e9n\u00e9ral Leclerc, 94275 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S20","DOI":"10.1016\/j.ajic.2006.05.238","article-title":"Resistance in gram-negative bacteria: Enterobacteriaceae","volume":"34","author":"Paterson","year":"2006","journal-title":"Am. 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