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This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2\/16) for samples collected 0\u20134 days post symptoms onset (DPSO), and of 75.0% (3\/4) for samples collected 5\u20139 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10\/11), 100% (2\/2), and 0% (0\/2) for samples obtained 12\u2013102, 258\u2013260, and 722\u2013727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate.<\/jats:p>","DOI":"10.3390\/diagnostics10100835","type":"journal-article","created":{"date-parts":[[2020,10,18]],"date-time":"2020-10-18T21:26:06Z","timestamp":1603056366000},"page":"835","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection"],"prefix":"10.3390","volume":"10","author":[{"given":"Moyra","family":"Machado Portilho","sequence":"first","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador, BA 40296-710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9095-5860","authenticated-orcid":false,"given":"Laise","family":"de Moraes","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador, BA 40296-710, Brazil"},{"name":"Faculdade de Medicina, Universidade Federal da Bahia, Salvador, BA 40110-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8119-7236","authenticated-orcid":false,"given":"Mariana","family":"Kikuti","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador, BA 40296-710, Brazil"},{"name":"Instituto de Sa\u00fade Coletiva, Universidade Federal da Bahia, Salvador, BA 40110-040, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leile Camila","family":"Jacob Nascimento","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador, BA 40296-710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitermayer","family":"Galv\u00e3o Reis","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador, BA 40296-710, Brazil"},{"name":"Faculdade de Medicina, Universidade Federal da Bahia, Salvador, BA 40110-100, Brazil"},{"name":"Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06520, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7241-6844","authenticated-orcid":false,"given":"Viviane","family":"Sampaio Boaventura","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador, BA 40296-710, Brazil"},{"name":"Faculdade de Medicina, Universidade Federal da Bahia, Salvador, BA 40110-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Khouri","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador, BA 40296-710, Brazil"},{"name":"Faculdade de Medicina, Universidade Federal da Bahia, Salvador, BA 40110-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6798-2059","authenticated-orcid":false,"given":"Guilherme","family":"Sousa Ribeiro","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador, BA 40296-710, Brazil"},{"name":"Faculdade de Medicina, Universidade Federal da Bahia, Salvador, BA 40110-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/S0140-6736(16)00562-6","article-title":"Guillain-Barr\u00e9 Syndrome outbreak caused by ZIKA virus infection in French Polynesia: A case-control study","volume":"387","author":"Blake","year":"2016","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1016\/S1473-3099(16)30318-8","article-title":"Association between Zika virus infection and microcephaly in Brazil, January to May, 2016: Preliminary report of a case-control study","volume":"16","author":"Rodrigues","year":"2016","journal-title":"Lancet Infect. 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