{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:09:59Z","timestamp":1790312999294,"version":"4.1.0"},"reference-count":19,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Accurate measurement of the hepatitis B virus (HBV) DNA is important for the management of patients with chronic HBV infection. Here, the performance of the Xpert\u00ae HBV Viral Load test (Xpert HBV Viral Load) versus the Roche COBAS\u00ae Ampliprep\/COBAS\u00ae TaqMan\u00ae system (CAP\/CTM HBV) HBV test v2.0 was evaluated. From September 2017 to December 2017, a total of 876 prospectively collected or archived serum or EDTA plasma specimens from subjects chronically infected with HBV were tested using the Xpert HBV Viral Load and the CAP\/CTM HBV v2.0 assays. Of the 876 specimens tested, 560 were within the quantitative range of both assays. The agreement between the two methods was 90.0%. No difference in plasma or serum samples was observed. Deming regression analysis showed a good correlation of the Xpert HBV Viral Load assay with the CAP\/CTM HBV v2.0 assay. The Bland\u2013Altman analysis showed a good agreement between the results of the Xpert HBV Viral Load assay and the CAP\/CTM HBV assay, with a mean difference (\u00b11.96 standard deviation) of 0.0091 \u00b1 0.3852 Log IU\/mL. Comparing the two assays, only nineteen specimens (2.1%) had a difference greater than 1.96 times the standard deviation. The Xpert\u00ae HBV Viral Load test is suitable for monitoring patients with HBV infection and is useful in diagnostic settings.<\/jats:p>","DOI":"10.3390\/diagnostics11020297","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T22:36:31Z","timestamp":1613169391000},"page":"297","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Multicenter Evaluation of the Cepheid Xpert\u00ae HBV Viral Load Test"],"prefix":"10.3390","volume":"11","author":[{"given":"Fabbio","family":"Marcuccilli","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Virology Unit, Polyclinic Tor Vergata Foundation, Viale Oxford, 81, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6913-9017","authenticated-orcid":false,"given":"Stephane","family":"Chevaliez","sequence":"additional","affiliation":[{"name":"National Reference Center for Viral Hepatitis B, C and Delta, Department of Virology, H\u00f4pital Henri Mondor, Universit\u00e9 Paris-Est, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Muller","sequence":"additional","affiliation":[{"name":"Medizinisches Versorgungszentrum Labor M\u00fcnchen Zentrum GbR, SYNLAB MVZ, 92637 Weiden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luna","family":"Colagrossi","sequence":"additional","affiliation":[{"name":"Area of Multimodal Medicine and Laboratory, Children Hospital Bambino Ges\u00f9, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Abbondanza","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Virology Unit, Polyclinic Tor Vergata Foundation, Viale Oxford, 81, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Beyser","sequence":"additional","affiliation":[{"name":"Medizinisches Versorgungszentrum Labor M\u00fcnchen Zentrum GbR, SYNLAB MVZ, 92637 Weiden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Wlassow","sequence":"additional","affiliation":[{"name":"National Reference Center for Viral Hepatitis B, C and Delta, Department of Virology, H\u00f4pital Henri Mondor, Universit\u00e9 Paris-Est, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Ortonne","sequence":"additional","affiliation":[{"name":"National Reference Center for Viral Hepatitis B, C and Delta, Department of Virology, H\u00f4pital Henri Mondor, Universit\u00e9 Paris-Est, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo Federico","family":"Perno","sequence":"additional","affiliation":[{"name":"Area of Multimodal Medicine and Laboratory, Children Hospital Bambino Ges\u00f9, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9943-9130","authenticated-orcid":false,"given":"Marco","family":"Ciotti","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Virology Unit, Polyclinic Tor Vergata Foundation, Viale Oxford, 81, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"EASL (2012). 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