{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T17:47:59Z","timestamp":1793468879298,"version":"4.5.0"},"reference-count":27,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T00:00:00Z","timestamp":1613692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute of Clinical Medicine Research, Catholic University of Korea, Yeouido St. Mary\u2019s Hospital","award":["2019"],"award-info":[{"award-number":["2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Diffuse large B-cell lymphoma (DLBCL) is the most common high-grade B-cell lymphoma found in Korea; it manifests with a variety of cellular morphologies and a high proliferation index. It is difficult to differentiate between DLBCL and Burkitt lymphoma (BL) based on immunohistochemistry, histology, and Epstein-Barr virus infection status owing to the overlap in findings. In this study, we performed comparative morphometric analysis to understand the proportional difference in Ki-67 staining between DLBCL and BL. We analyzed Ki-67-stained slides of 103 DLBCLs and 29 BLs that were pathologically confirmed using a three-tier classification system (negative, 1+, 2+, and 3+) to compare Ki-67 expression between BL and activated B-cell and germinal center B-cell subtypes of DLBCL and DLBCL with high proliferation indices (&gt;90% of 2+ and 3+ cells). Patients with DLBCL were older than those with BL (62.1 versus 51.0 years). The number and proportion of negative cells (passenger and true negative cells) were significantly lower in BLs than those in DLBCLs (337.4, 5.9% versus 690.3, 12.4%). The number and proportion of 3+ cells were significantly higher in BLs than those in DLBCLs (5213.6, 96.3% versus 3132.4, 62.0%). BLs and DLBCLs with a high proliferation index showed similar results as those between BLs and overall DLBCLs. We were able to differentiate BLs and DLBCLs with 98.1% sensitivity and 100.0% specificity using an optimal cut-off of 97.9% of 2+\/3+ Ki-67-positive cells. Thus, the Ki-67 labeling index may be a good differential biomarker for DLBCLs and BLs.<\/jats:p>","DOI":"10.3390\/diagnostics11020343","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T09:06:10Z","timestamp":1613725570000},"page":"343","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8615-3064","authenticated-orcid":false,"given":"Yosep","family":"Chong","sequence":"first","affiliation":[{"name":"Department of Hospital Pathology, Yeouido St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Seoul 07345, Korea"},{"name":"Department of Hospital Pathology, Uijeongbu St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Uijeongbu 11765, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology, Incheon St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Incheon 21431, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6229-8418","authenticated-orcid":false,"given":"Uiju","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology, St. Vincent\u2019s Hospital, The Catholic University of Korea College of Medicine, Suwon 16247, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Sun","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology, Bucheon St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Bucheon 14647, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8767-9033","authenticated-orcid":false,"given":"Kwangil","family":"Yim","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology, Uijeongbu St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Uijeongbu 11765, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishant","family":"Thakur","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology, Yeouido St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Seoul 07345, Korea"},{"name":"Department of Hospital Pathology, Uijeongbu St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Uijeongbu 11765, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Ok","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology, Daejeon St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Daejeon 35301, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inju","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology, Yeouido St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Seoul 07345, Korea"},{"name":"Department of Hospital Pathology, Eunpyeong St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Seoul 03312, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyeongsin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology, Seoul St. Mary\u2019s Hospital, The Catholic University of Korea College of Medicine, Seoul 06591, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,19]]},"reference":[{"key":"ref_1","unstructured":"Campo, E., Harris, N.L., and Pileri, S.A. 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