{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:07:35Z","timestamp":1790687255140,"version":"4.1.0"},"reference-count":21,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002738","name":"Assistance Publique - H\u00f4pitaux de Paris","doi-asserted-by":"publisher","award":["Bacteriologie Bicetre"],"award-info":[{"award-number":["Bacteriologie Bicetre"]}],"id":[{"id":"10.13039\/501100002738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007241","name":"University of Paris-Saclay","doi-asserted-by":"publisher","award":["1184"],"award-info":[{"award-number":["1184"]}],"id":[{"id":"10.13039\/501100007241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Inserm","doi-asserted-by":"publisher","award":["1184"],"award-info":[{"award-number":["1184"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-33"],"award-info":[{"award-number":["ANR-10-LABX-33"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010686","name":"European Institute of Innovation and Technology","doi-asserted-by":"publisher","award":["AMR Detectool"],"award-info":[{"award-number":["AMR Detectool"]}],"id":[{"id":"10.13039\/100010686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Vancomycin-resistant enterococci (VREs) have become one of the most important nosocomial pathogens worldwide, associated with increased treatment costs, prolonged hospital stays and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VREs) using 104 well-characterized enterococcal isolates. The sensitivity and specificity were both 100% when bacterial cells were grown in the presence of vancomycin used as a VanB inducer. The NG-Test VanB is an efficient, rapid and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs from colonies. Together with the NG-Test VanA, they could replace the already existing tests available for the confirmation of acquired vancomycin resistance in enterococci, especially from selective media or from antibiograms, with 100% sensitivity and specificity. Rapid detection in less than 15 min will result in more efficient management of carriers and infected patients. In addition, these tests may be used for positive blood cultures, given a 3.5 h sub-culturing step on Chocolate agar PolyViteX in the presence of a 5-\u00b5g vancomycin disk, which is routinely performed in many clinical microbiology laboratories for every positive blood culture for subsequent MALDI-TOF identification of the growing bacteria.<\/jats:p>","DOI":"10.3390\/diagnostics11101805","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:03:42Z","timestamp":1632960222000},"page":"1805","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid Detection of VanA\/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5766-3831","authenticated-orcid":false,"given":"Saoussen","family":"Oueslati","sequence":"first","affiliation":[{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Service de Bact\u00e9riologie-Hygi\u00e8ne, Bic\u00eatre Hospital, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Team ReSIST, INSERM U1184, School of Medicine Universit\u00e9 Paris-Saclay, LabEx LERMIT, 78 Rue du G\u00e9n\u00e9ral Leclerc, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Service de Bact\u00e9riologie-Hygi\u00e8ne, Bic\u00eatre Hospital, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Team ReSIST, INSERM U1184, School of Medicine Universit\u00e9 Paris-Saclay, LabEx LERMIT, 78 Rue du G\u00e9n\u00e9ral Leclerc, 94270 Le Kremlin-Bic\u00eatre, 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