{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:49:32Z","timestamp":1783385372697,"version":"3.54.6"},"reference-count":38,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T00:00:00Z","timestamp":1634688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HR20C0021"],"award-info":[{"award-number":["HR20C0021"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"name":"government-wide R&amp;D fund for infectious disease research","award":["HG18C0012"],"award-info":[{"award-number":["HG18C0012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Malaria, caused by the parasite Plasmodium and transmitted by mosquitoes, is an epidemic that mainly occurs in tropical and subtropical regions. As treatments differ across species of malarial parasites, there is a need to develop rapid diagnostic methods to differentiate malarial species. Herein, we developed a multiplex malaria Pan\/Pf\/Pv\/actin beta loop-mediated isothermal amplification (LAMP) to diagnose Plasmodium spp., P. falciparum, and P. vivax, as well as the internal control (IC), within 40 min. The detection limits of the multiplex malaria Pan\/Pf\/Pv\/IC LAMP were 1 \u00d7 102, 1 \u00d7 102, 1 \u00d7 102, and 1 \u00d7 103 copies\/\u00b5L for four vectors, including the 18S rRNA gene (Plasmodium spp.), lactate dehydrogenase gene (P. falciparum), 16S rRNA gene (P. vivax), and human actin beta gene (IC), respectively. The performance of the LAMP assay was compared and evaluated by evaluating 208 clinical samples (118 positive and 90 negative samples) with the commercial RealStar\u00ae Malaria S&amp;T PCR Kit 1.0. The developed multiplex malaria Pan\/Pf\/Pv\/IC LAMP assay showed comparable sensitivity (100%) and specificity (100%) with the commercial RealStar\u00ae Malaria S&amp;T PCR Kit 1.0 (100%). These results suggest that the multiplex malaria Pan\/Pf\/Pv\/IC LAMP could be used as a point-of-care molecular diagnostic test for malaria.<\/jats:p>","DOI":"10.3390\/diagnostics11111950","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T21:29:46Z","timestamp":1634765386000},"page":"1950","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development of a Multiplex Loop-Mediated Isothermal Amplification Assay for Diagnosis of Plasmodium spp., Plasmodium falciparum and Plasmodium vivax"],"prefix":"10.3390","volume":"11","author":[{"given":"Woong Sik","family":"Jang","sequence":"first","affiliation":[{"name":"Emergency Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"},{"name":"Departments of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da Hye","family":"Lim","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YoungLan","family":"Choe","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunseul","family":"Jee","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Chul","family":"Moon","sequence":"additional","affiliation":[{"name":"Emergency Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaewon","family":"Kim","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minkyeong","family":"Choi","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In Su","family":"Park","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7765-0840","authenticated-orcid":false,"given":"Chae Seung","family":"Lim","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4670","DOI":"10.1111\/j.1742-4658.2007.05997.x","article-title":"Malaria\u2014An overview","volume":"274","author":"Tuteja","year":"2007","journal-title":"FEBS J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/978-1-4939-9550-9_2","article-title":"Current Situation of Malaria in Africa","volume":"2013","author":"Mbacham","year":"2019","journal-title":"Methods Mol. 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