{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:01:10Z","timestamp":1779102070205,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001713","name":"European &amp; Developing Countries Clinical Trials Partnership","doi-asserted-by":"publisher","award":["DRIA2014-308 SOLID"],"award-info":[{"award-number":["DRIA2014-308 SOLID"]}],"id":[{"id":"10.13039\/501100001713","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["01KA1617"],"award-info":[{"award-number":["01KA1617"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>The lack of cheap, easy-to-use, rapid diagnostic tests has led to the development of several rapid diagnostic tests for cysticercosis. The new prototype two-strip, Taenia solium point of care test (TS POC) detects antibodies against taeniosis (TS POC T) and cysticercosis (TS POC CC). This study evaluated the diagnostic performance of the TS POC CC in the Sinda district in eastern Zambia. A sample of 1254 participants was recruited and tested with the TS POC. Out of the 1249 participants with a valid TS POC result, 177 (14%) tested positive while 1072 (86%) tested negative. All individuals with a positive TS POC and a subset of negative TS POC participants were selected for serum sampling, and were subjected to the recombinant glycoprotein T24H enzyme-linked immunoelectrotransfer blot (rT24H EITB) and the serum B60\/158 (serum Ag) enzyme-linked immunosorbent assay (Ag ELISA). Performance characteristics were estimated using a Bayesian approach with probabilistic constraints. Based on 255 complete cases, the estimated sensitivity and specificity of the TS POC CC test were 35% (95% CI: 14\u201363%) and 87% (95% CI: 83\u201390%), respectively. The diagnostic performance needs to be improved, possibly by titrating antigen and other reagents\u2019 concentration in the strip to produce a performance similar to existing cysticercosis tests such as the rT24H EITB.<\/jats:p>","DOI":"10.3390\/diagnostics11112121","type":"journal-article","created":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T20:46:47Z","timestamp":1637009207000},"page":"2121","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of Taenia solium Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9093-3012","authenticated-orcid":false,"given":"Chishimba","family":"Mubanga","sequence":"first","affiliation":[{"name":"Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, 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Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6108-2586","authenticated-orcid":false,"given":"Veronika","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Neurology, Center for Global Health, Faculty of Medicine, Technical University of Munich, 81675 Munich, Germany"},{"name":"Centre for Global Health, Institute of Health and Society, Faculty of Medicine, University of Oslo, 0450 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isaac K.","family":"Phiri","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1195-0453","authenticated-orcid":false,"given":"Gideon","family":"Zulu","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia"},{"name":"Eastern Provincial Health Office, Ministry of Health, Chipata 510023, Zambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Noh","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sukwan","family":"Handali","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Mambo","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia"},{"name":"Eastern Provincial Health Office, Ministry of Health, Chipata 510023, Zambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mwelwa","family":"Chembensofu","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxwell","family":"Masuku","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dries","family":"Reynders","sequence":"additional","affiliation":[{"name":"Department of Applied Mathematics, Computer Science and Statistics, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Famke","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5868-7760","authenticated-orcid":false,"given":"Emmanuel","family":"Bottieau","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7372-0250","authenticated-orcid":false,"given":"Pascal","family":"Magnussen","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea S.","family":"Winkler","sequence":"additional","affiliation":[{"name":"Department of Neurology, Center for Global Health, Faculty of Medicine, Technical University of Munich, 81675 Munich, Germany"},{"name":"Centre for Global Health, Institute of Health and Society, Faculty of Medicine, University of Oslo, 0450 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5381-7987","authenticated-orcid":false,"given":"Pierre","family":"Dorny","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6788-2592","authenticated-orcid":false,"given":"Kabemba E.","family":"Mwape","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2523-233X","authenticated-orcid":false,"given":"Sarah","family":"Gabri\u00ebl","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, 9820 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(2015). Taenia Solium Human Cysticercosis: A Systematic Review of Sero-Epidemiological Data from Endemic Zones around the World. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003919"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1017\/S1466252311000077","article-title":"Human and Porcine Taenia Solium Infections in Mozambique: Identifying Research Priorities","volume":"12","author":"Afonso","year":"2011","journal-title":"Anim. Health Res. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1056\/NEJMc1909955","article-title":"Potential Elimination of Active Taenia Solium Transmission in Africa","volume":"383","author":"Mwape","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1590\/S1726-46342010000400016","article-title":"Epidemiology and Control of Cysticercosis in Peru","volume":"27","author":"Garcia","year":"2010","journal-title":"Rev. Peru Med. Exp. Salud Publica"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1111\/j.1528-1167.2010.02945.x","article-title":"Reduction in Rate of Epilepsy from Neurocysticercosis by Community Interventions: The Salam\u00e1, Honduras Study","volume":"52","author":"Medina","year":"2011","journal-title":"Epilepsia"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0035-9203(00)90451-6","article-title":"Mass Treatment against Human Taeniasis for the Control of Cysticercosis: A Population-Based Intervention Study","volume":"94","author":"Sarti","year":"2000","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1179\/2047773212Y.0000000048","article-title":"Immunological and Molecular Diagnosis of Cysticercosis","volume":"106","author":"Rodriguez","year":"2012","journal-title":"Pathog. Glob. Health"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1093\/infdis\/159.1.50","article-title":"An Enzyme-Linked Immunoelectrotransfer Blot Assay and Glycoprotein Antigens for Diagnosing Human Cysticercosis (Taenia solium)","volume":"159","author":"Tsang","year":"1989","journal-title":"J. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"413","DOI":"10.4269\/ajtmh.2010.10-0121","article-title":"Infection with versus Exposure to Taenia Solium: What Do Serological Test Results Tell Us?","volume":"83","author":"Praet","year":"2010","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JCM.00424-18","article-title":"Laboratory Diagnosis of Neurocysticercosis (Taenia solium)","volume":"56","author":"Garcia","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.molbiopara.2006.02.004","article-title":"Characterization and Cloning of T24, a Taenia Solium Antigen Diagnostic for Cysticercosis","volume":"147","author":"Hancock","year":"2006","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"565","DOI":"10.4269\/ajtmh.17-0541","article-title":"Evaluating the Recombinant T24H Enzyme-Linked Immunoelectrotransfer Blot Assay for the Diagnosis of Neurocysticercosis in a Panel of Samples from a Large Community-Based Randomized Control Trial in 60 Villages in Burkina Faso","volume":"98","author":"Dermauw","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.ijpara.2003.11.014","article-title":"A Bayesian Approach for Estimating Values for Prevalence and Diagnostic Test Characteristics of Porcine Cysticercosis","volume":"34","author":"Dorny","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1136\/jnnp.2006.107243","article-title":"Detection of HP10 Antigen in Serum for Diagnosis and Follow-up of Subarachnoidal and Intraventricular Human Neurocysticercosis","volume":"78","author":"Fleury","year":"2007","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jns.2016.11.045","article-title":"Revised Diagnostic Criteria for Neurocysticercosis","volume":"372","author":"Nash","year":"2017","journal-title":"J. Neurol. Sci."},{"key":"ref_16","unstructured":"World Health Organization (2015). Taenia Solium Taeniasis\/Cysticercosis Diagnostic Tools, WHO."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Pai, N.P., Vadnais, C., Denkinger, C., Engel, N., and Pai, M. (2012). Point-of-Care Testing for Infectious Diseases: Diversity, Complexity, and Barriers in Low- And Middle-Income Countries. PLoS Med., 9.","DOI":"10.1371\/journal.pmed.1001306"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.actatropica.2019.03.030","article-title":"Progress on the Development of Rapid Diagnostic Tests for Foodborne Neglected Zoonotic Helminthiases: A Systematic Review","volume":"194","author":"Mubanga","year":"2019","journal-title":"Acta Trop."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1645\/GE-189R","article-title":"Characterization, Cloning, and Expression of Two Diagnostic Antigens for Taenia Solium Tapeworm Infection","volume":"90","author":"Levine","year":"2004","journal-title":"J. Parasitol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Van Damme, I., Trevisan, C., Mwape, K.E., Schmidt, V., Magnussen, P., Zulu, G., Mubanga, C., Stelzle, D., Bottieau, E., and Abatih, E.N. (2021). Trial Design for a Diagnostic Accuracy Study of a Point-of-Care Test for the Detection of Taenia Solium Taeniosis and (Neuro)Cysticercosis in Community Settings of Highly Endemic, Resource-Poor Areas in Zambia: Challenges and Rationale. Diagnostics, 11.","DOI":"10.3390\/diagnostics11071138"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e00082","DOI":"10.1016\/j.parepi.2018.e00082","article-title":"Taenia Solium Control in Zambia: The Potholed Road to Success","volume":"4","author":"Mwape","year":"2019","journal-title":"Parasite Epidemiol. Control"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Mubanga, C., Mwape, K.E., Phiri, I.K., Trevisan, C., Kabululu, M., Zulu, G., Van Damme, I., Schmidt, V., Dorny, P., and Gabri\u00ebl, S. (2021). Operational Characteristics of an Antibody Detecting Point of Care Test for Taenia Solium Infections in a Community and Hospital Setting. BMC Infect. Dis., 21.","DOI":"10.1186\/s12879-021-06320-3"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1128\/JCM.03260-13","article-title":"Recombinant Protein- and Synthetic Peptide-Based Immunoblot Test for Diagnosis of Neurocysticercosis","volume":"52","author":"Noh","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vetpar.2007.05.010","article-title":"Bayes and Diagnostic Testing","volume":"148","author":"Lesaffre","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1097\/01.ede.0000198422.64801.8d","article-title":"Estimating Disease Prevalence in a Bayesian Framework Using Probabilistic Constraints","volume":"17","author":"Berkvens","year":"2006","journal-title":"Epidemiology"},{"key":"ref_26","unstructured":"Nakazawa, M. (2021, February 21). Fmsb: Functions for Medical Statistics Book with Some Demographic Data. R Package Version 0.5.1. Available online: http:\/\/CRAN.Rproject.Org\/Package=fmsb."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/0895-4356(90)90158-L","article-title":"High Agreement but Low Kappa: I. the Problems of Two Paradoxes","volume":"43","author":"Feinstein","year":"1990","journal-title":"J. Clin. Epidemiol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Donadeu, M., Fahrion, A.S., Olliaro, P.L., and Abela-Ridder, B. (2017). Target Product Profiles for the Diagnosis of Taenia Solium Taeniasis, Neurocysticercosis and Porcine Cysticercosis. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005875"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/pim.12291","article-title":"Monitoring the Outcomes of Interventions against Taenia Solium: Options and Suggestions","volume":"38","author":"Lightowlers","year":"2016","journal-title":"Parasite Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1186\/s13071-017-2160-2","article-title":"Comparison of T24H-His, GST-T24H and GST-Ts8B2 Recombinant Antigens in Western Blot, ELISA and Multiplex Bead-Based Assay for Diagnosis of Neurocysticercosis","volume":"10","author":"Noh","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.trstmh.2011.05.010","article-title":"Field Evaluation of Urine Antigen Detection for Diagnosis of Taenia Solium Cysticercosis","volume":"105","author":"Mwape","year":"2011","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gass, K. (2020). Time for a Diagnostic Sea-Change: Rethinking Neglected Tropical Disease Diagnostics to Achieve Elimination. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008933"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Mwape, K.E., Phiri, I.K., Praet, N., Speybroeck, N., Muma, J.B., Dorny, P., and Gabriel, S. (2013). The Incidence of Human Cysticercosis in a Rural Community of Eastern Zambia. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002142"},{"key":"ref_34","first-page":"1","article-title":"High Frequency of Taenia Solium Antigen Positivity in Patients Admitted for Neurological Disorders in the Rural Hospital of Mosango, Democratic Republic of Congo","volume":"21","author":"Mukendi","year":"2020","journal-title":"BMC Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Umemneku Chikere, C.M., Wilson, K., Graziadio, S., Vale, L., and Allen, A.J. (2019). Diagnostic Test Evaluation Methodology: A Systematic Review of Methods Employed to Evaluate Diagnostic Tests in the Absence of Gold Standard\u2014An Update. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0223832"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1111\/j.1365-2672.2008.03902.x","article-title":"A Bayesian Modelling Framework to Estimate Campylobacter Prevalence and Culture Methods Sensitivity: Application to a Chicken Meat Survey in Belgium","volume":"105","author":"Habib","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.vetpar.2018.03.013","article-title":"Estimating Prevalence and Diagnostic Test Characteristics of Bovine Cysticercosis in Belgium in the Absence of a \u2018Gold Standard\u2019 Reference Test Using a Bayesian Approach","volume":"254","author":"Jansen","year":"2018","journal-title":"Vet. Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1111\/tmi.12089","article-title":"Bayesian Modelling to Estimate the Test Characteristics of Coprology, Coproantigen ELISA and a Novel Real-Time PCR for the Diagnosis of Taeniasis","volume":"18","author":"Praet","year":"2013","journal-title":"Trop. Med. Int. Health"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ng-Nguyen, D., Stevenson, M.A., Dorny, P., Gabri\u00ebl, S., Van Vo, T., Nguyen, V.-A.T., Van Phan, T., Hii, S.F., and Traub, R.J. (2017). Comparison of a New Multiplex Real-Time PCR with the Kato Katz Thick Smear and Copro-Antigen ELISA for the Detection and Differentiation of Taenia Spp. in Human Stools. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005743"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Speybroeck, N., Praet, N., Claes, F., van Hong, N., Torres, K., Mao, S., van den Eede, P., Thinh, T.T., Gamboa, D., and Sochantha, T. (2011). True versus Apparent Malaria Infection Prevalence: The Contribution of a Bayesian Approach. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0016705"}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/11\/11\/2121\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:30:45Z","timestamp":1760167845000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/11\/11\/2121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,15]]},"references-count":40,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["diagnostics11112121"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics11112121","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,15]]}}}