{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:37:01Z","timestamp":1760236621193,"version":"build-2065373602"},"reference-count":29,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T00:00:00Z","timestamp":1639094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: Monitoring inflammatory bowel disease patients may be challenging. Fecal calprotectin is one of the most performed tests. Other fecal biomarkers are less used in clinical practice. Rapid fecal tests that could be performed by patients may be a useful strategy to closely monitor disease activity. Methods: We performed a prospective observational study including consecutive inflammatory bowel disease patients referred for colonoscopy in a single center. Certest FOB + Transferrin + Calprotectin + Lactoferrin\u00ae (Certest Biotec S.L, Zaragoza, Spain), a one-step point-of-care test which simultaneously detects these four biomarkers was performed. Endoscopic inflammatory activity was defined using the Mayo score (\u22651) in ulcerative colitis, SES-CD (&gt;3) and Rutgeerts scores (\u22651) for Crohn\u2019s disease. Results: Out of a total of 106 patients (56.5% female, mean age 51 years), 54 (50.9%) were diagnosed with ulcerative colitis and 52 (49.1%) with Crohn\u2019s disease. Endoscopic activity was detected in 42 patients (39.0%). Fecal calprotectin provided the best sensitivity (97.6%), with limited specificity (34.4%). Compared to calprotectin, the other 3 fecal biomarkers showed better specificity (87.5\u201392.1%) and lower sensitivity (45.2\u201359.5%). Patients with a negative result in all biomarkers (19\/106\u201417.9%) had 100% (CI 95% 97.4\u2013100) negative predictive value, while patients with the 4 biomarkers positive (13\/106\u201412.3%) had 100% (CI 95% 96.1\u2013100) positive predictive value of endoscopic inflammatory activity. AUROC of this 4 biomarker point-of-care test was 0.845 (95% CI 0.771\u20130.920), significantly higher than the AUROCs of any of the 4 biomarkers. Conclusions: This test may be a useful strategy to monitor inflammatory activity in clinical practice by excluding or prioritizing patients in need of a colonoscopy.<\/jats:p>","DOI":"10.3390\/diagnostics11122323","type":"journal-article","created":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T02:07:18Z","timestamp":1639102038000},"page":"2323","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Patient Self-Made Point-of-Care Fecal Test Improves Diagnostic Accuracy Compared with Fecal Calprotectin Alone in Inflammatory Bowel Disease Patients"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-9593","authenticated-orcid":false,"given":"Gonzalo","family":"Hijos-Mallada","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, University Hospital \u201cLozano Blesa\u201d, 50009 Zaragoza, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Arag\u00f3n), 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raul","family":"Velamaz\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, University Hospital \u201cLozano Blesa\u201d, 50009 Zaragoza, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Arag\u00f3n), 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal","family":"Marti","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Zaragoza, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3644-9240","authenticated-orcid":false,"given":"Eduardo","family":"Chueca","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Arag\u00f3n), 50009 Zaragoza, Spain"},{"name":"CIBERehd, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8575-0414","authenticated-orcid":false,"given":"Samantha","family":"Arechavaleta","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Arag\u00f3n), 50009 Zaragoza, Spain"},{"name":"CIBERehd, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9672-6038","authenticated-orcid":false,"given":"Alberto","family":"Lu\u00e9","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, University Hospital \u201cLozano Blesa\u201d, 50009 Zaragoza, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Arag\u00f3n), 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0076-3529","authenticated-orcid":false,"given":"Fernando","family":"Gomoll\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, University Hospital \u201cLozano Blesa\u201d, 50009 Zaragoza, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Arag\u00f3n), 50009 Zaragoza, Spain"},{"name":"Department of Medicine, University of Zaragoza, 50009 Zaragoza, Spain"},{"name":"CIBERehd, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5932-2889","authenticated-orcid":false,"given":"Angel","family":"Lanas","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, University Hospital \u201cLozano Blesa\u201d, 50009 Zaragoza, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Arag\u00f3n), 50009 Zaragoza, Spain"},{"name":"Department of Medicine, University of Zaragoza, 50009 Zaragoza, Spain"},{"name":"CIBERehd, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Sostres","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, University Hospital \u201cLozano Blesa\u201d, 50009 Zaragoza, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Arag\u00f3n), 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/nrgastro.2017.110","article-title":"The IBD interactome: An integrated view of aetiology, pathogenesis and therapy","volume":"14","author":"Fiocchi","year":"2017","journal-title":"Nat. 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