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Loa loa has been associated with severe adverse reactions in high Loa-infected individuals receiving ivermectin during mass drug administration programs for the control of onchocerciasis and lymphatic filariasis. Diagnosis of loiasis still depends on microscopy in blood samples, but this is not effective for large-scale surveys. New diagnostics methods for loiasis are urgently needed. Previously, we developed a colorimetric high-sensitive and species-specific LAMP for Loa loa DNA detection. Here, we evaluate it in a set of 100 field-collected clinical samples stored as dried blood spots. In addition, Loa loa-LAMP was also evaluated in real-time testing and compared with microscopy and a specific PCR\/nested PCR. A simple saponin\/Chelex-based method was used to extract DNA. Colorimetric and real-time LAMP assays detected more samples with microscopy-confirmed Loa loa and Loa loa\/Mansonella perstans mixed infections than PCR\/nested-PCR. Samples with the highest Loa loa microfilariae counts were amplified faster in real-time LAMP assays. Our Loa loa-LAMP could be a promising molecular tool for the easy, rapid and accurate screening of patients for loiasis in endemic areas with low-resource settings. The real-time testing (feasible in a handheld device) could be very useful to rule out high-microfilariae loads in infected patients.<\/jats:p>","DOI":"10.3390\/diagnostics12051079","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T21:16:28Z","timestamp":1650921388000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Colorimetric and Real-Time Loop-Mediated Isothermal Amplification (LAMP) for Detection of Loa loa DNA in Human Blood Samples"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5940-6572","authenticated-orcid":false,"given":"Bego\u00f1a","family":"Febrer-Sendra","sequence":"first","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37008 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4089-4307","authenticated-orcid":false,"given":"Pedro","family":"Fern\u00e1ndez-Soto","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37008 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Crego-Vicente","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37008 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6947-6806","authenticated-orcid":false,"given":"Juan Garc\u00eda-Bernalt","family":"Diego","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37008 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thuy-Huong","family":"Ta-Tang","sequence":"additional","affiliation":[{"name":"Malaria and Neglected Tropical Diseases Laboratory, National Centre of Tropical Medicine, CIBERINFEC, Institute of Health Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Berzosa","sequence":"additional","affiliation":[{"name":"Malaria and Neglected Tropical Diseases Laboratory, National Centre of Tropical Medicine, CIBERINFEC, Institute of Health Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rufino","family":"Nguema","sequence":"additional","affiliation":[{"name":"National Control Programme of Onchocerciasis and Other Filariasis, Ministry of Health, Malabo, Equatorial Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Policarpo","family":"Ncogo","sequence":"additional","affiliation":[{"name":"National Control Programme of Onchocerciasis and Other Filariasis, Ministry of Health, Malabo, Equatorial Guinea"},{"name":"Fundaci\u00f3n Estatal, Salud, Infancia y Bienestar Social, Institute of Health Carlos III (CSAI\/ISCIII), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0177-6885","authenticated-orcid":false,"given":"Mar\u00eda","family":"Romay-Barja","sequence":"additional","affiliation":[{"name":"Malaria and Neglected Tropical Diseases Laboratory, National Centre of Tropical Medicine, CIBERINFEC, Institute of Health Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6508-3818","authenticated-orcid":false,"given":"Zaida","family":"Herrador","sequence":"additional","affiliation":[{"name":"Malaria and Neglected Tropical Diseases Laboratory, National Centre of Tropical Medicine, CIBERINFEC, Institute of Health Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agust\u00edn","family":"Benito","sequence":"additional","affiliation":[{"name":"Malaria and Neglected Tropical Diseases Laboratory, National Centre of Tropical Medicine, CIBERINFEC, Institute of Health Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0244-4740","authenticated-orcid":false,"given":"Antonio","family":"Muro","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37008 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1179\/136485906X112194","article-title":"Loiasis","volume":"8","author":"Boussinesq","year":"2006","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S1473-3099(13)70263-9","article-title":"Loa loa-does it deserve to be neglected?","volume":"14","author":"Metzger","year":"2014","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Gobbi, F., Bottieau, E., Bouchaud, O., Buonfrate, D., Salvador, F., Rojo-Marcos, G., Rodari, P., Clerinx, J., Trevi\u00f1o, B., and Herrera-\u00c1vila, J.P. (2018). Comparison of different drug regimens for the treatment of loiasis\u2014A TropNet retrospective study. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006917"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1093\/cid\/cix877","article-title":"Familial aggregation and heritability of loa loa microfilaremia","volume":"66","author":"Eyebe","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"ref_5","unstructured":"(2021, November 23). Expanded Special Project for Elimination of NTDs. Available online: https:\/\/espen.afro.who.int\/."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e00706-19","DOI":"10.1128\/JCM.00706-19","article-title":"Diagnostic identification and differentiation of microfilariae","volume":"57","author":"Mathison","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ojurongbe, O., Akindele, A.A., Adeleke, M.A., Oyedeji, M.O., Adedokun, S.A., Ojo, J.F., Akinleye, C.A., Bolaji, O.S., Adefioye, O.A., and Adeyeba, O.A. (2015). Co-endemicity of Loiasis and Onchocerciasis in Rain Forest Communities in Southwestern Nigeria. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003633"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S109","DOI":"10.1016\/j.actatropica.2010.01.014","article-title":"Mansonella perstans filariasis in Africa","volume":"120","author":"Simonsen","year":"2011","journal-title":"Acta Trop."},{"key":"ref_9","first-page":"9","article-title":"Mansonellosis: Current perspectives","volume":"9","author":"Crainey","year":"2018","journal-title":"Res. Rep. Trop. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1186\/s40249-020-00729-9","article-title":"Imported Mansonella perstans infection in Spain","volume":"9","author":"Puente","year":"2020","journal-title":"Infect. Dis. Poverty"},{"key":"ref_11","first-page":"286re4","article-title":"Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope","volume":"7","author":"Bakalar","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1056\/NEJMoa1705026","article-title":"A Test-and-Not-Treat Strategy for Onchocerciasis in Loa loa\u2013Endemic Areas","volume":"377","author":"Kamgno","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"116","DOI":"10.4269\/ajtmh.18-0163","article-title":"In Southern Nigeria Loa loa Blood Microfilaria Density is Very Low even in Areas with High Prevalence of Loiasis: Results of a Survey Using the New LoaScope Technology","volume":"99","author":"Emukah","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/S1473-3099(19)30554-7","article-title":"Implications for annual retesting after a test-and-not-treat strategy for onchocerciasis elimination in areas co-endemic with Loa loa infection: An observational cohort study","volume":"20","author":"Pion","year":"2020","journal-title":"Lancet Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Johnson, O., Giorgi, E., Fronterr\u00e8, C., Amoah, B., Atsame, J., Ella, S.N., Biamonte, M., Ogoussan, K., Hundley, L., and Gass, K. (2022). Geostatistical modelling enables efficient safety assessment for mass drug administration with ivermectin in Loa loa endemic areas through a combined antibody and LoaScope testing strategy for elimination of onchocerciasis. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010189"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1128\/JCM.00490-08","article-title":"Rapid, novel, specific, high-throughput assay for diagnosis of Loa loa infection","volume":"46","author":"Burbelo","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Wanji, S., Amvongo-Adjia, N., Koudou, B., Njouendou, A.J., Chounna Ndongmo, P.W., Kengne-Ouafo, J.A., Datchoua-Poutcheu, F.R., Fovennso, B.A., Tayong, D.B., and Fombad, F.F. (2015). Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0004184"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Pedram, B., Pasquetto, V., Drame, P.M., Ji, Y., Gonzalez-Moa, M.J., Baldwin, R.K., Nutman, T.B., and Biamonte, M.A. (2017). A novel rapid test for detecting antibody responses to Loa loa infections. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005741"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1006\/expr.1997.4168","article-title":"Detection of Loa loa-specific DNA in blood from occult-infected individuals","volume":"86","author":"Bain","year":"1997","journal-title":"Exp. Parasitol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Fink, D.L., Kamgno, J., and Nutman, T.B. (2011). Rapid molecular assays for specific detection and quantitation of Loa loa microfilaremia. PLoS Negl. Trop. Dis., 5.","DOI":"10.1371\/journal.pntd.0001299"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.actatropica.2017.12.030","article-title":"Geographical distribution and species identification of human filariasis and onchocerciasis in Bioko Island, Equatorial Guinea","volume":"180","author":"Moya","year":"2018","journal-title":"Acta Trop."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.pt.2015.04.006","article-title":"Expanding the MDx toolbox for filarial diagnosis and surveillance","volume":"31","author":"Alhassan","year":"2015","journal-title":"Trends Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12275-015-4656-9","article-title":"Loop-mediated isothermal amplification (LAMP): Principle, features, and future prospects","volume":"53","author":"Notomi","year":"2015","journal-title":"J. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/bbrc.2001.5921","article-title":"Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation","volume":"289","author":"Mori","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1038\/nprot.2008.57","article-title":"Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products","volume":"3","author":"Tomita","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Soto, P., Mvoulouga, P.O., Akue, J.P., Ab\u00e1n, J.L., Santiago, B.V., S\u00e1nchez, M.C., and Muro, A. (2014). Development of a highly sensitive Loop-Mediated Isothermal Amplification (LAMP) method for the detection of Loa loa. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0094664"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1128\/JCM.00525-14","article-title":"Loop-mediated isothermal amplification for rapid and semiquantitative detection of loa loa infection","volume":"52","author":"Drame","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Poole, C.B., Ettwiller, L., Tanner, N.A., Evans, T.C., Wanji, S., and Carlow, C.K.S. (2015). Genome filtering for new DNA biomarkers of Loa loa infection suitable for loop-mediated isothermal amplification. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0139286"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.parint.2011.08.018","article-title":"Development of loop-mediated isothermal amplification method for detecting Wuchereria bancrofti DNA in human blood and vector mosquitoes","volume":"60","author":"Takagi","year":"2011","journal-title":"Parasitol. Int."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Poole, C.B., Tanner, N.A., Zhang, Y., Evans, T.C., and Carlow, C.K.S. (2012). Diagnosis of Brugian Filariasis by Loop-Mediated Isothermal Amplification. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001948"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Alhassan, A., Makepeace, B.L., Lacourse, E.J., Osei-Atweneboana, M.Y., and Carlow, C.K.S. (2014). A simple isothermal DNA amplification method to screen black flies for Onchocerca volvulus infection. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0108927"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1186\/s13071-016-1913-7","article-title":"An isothermal DNA amplification method for detection of Onchocerca volvulus infection in skin biopsies","volume":"9","author":"Lagatie","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"10275","DOI":"10.1038\/s41598-019-46550-9","article-title":"In Silico Identification of Novel Biomarkers and Development of New Rapid Diagnostic Tests for the Filarial Parasites Mansonella perstans and Mansonella ozzardi","volume":"9","author":"Poole","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"195","DOI":"10.4269\/ajtmh.13-0463","article-title":"Review article: An overview of the clinical use of filter paper in the diagnosis of tropical diseases","volume":"90","author":"Smit","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"bpaa009","DOI":"10.1093\/biomethods\/bpaa009","article-title":"Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots","volume":"5","author":"Simon","year":"2020","journal-title":"Biol. Methods Protoc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"249","DOI":"10.4269\/ajtmh.2005.72.249","article-title":"Short report: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum","volume":"72","author":"Bereczky","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Schmidt, B., Xu, W., Gonz\u00e1lez, I.J., Polley, S.D., Bell, D., Shakely, D., Msellem, M.I., Bj\u00f6rkman, A., and M\u00e5rtensson, A. (2014). Loop mediated isothermal amplification (LAMP) accurately detects malaria DNA from filter paper blood samples of low density parasitaemias. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0103905"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1186\/s12936-018-2527-7","article-title":"Combination of PURE-DNA extraction and LAMP-DNA amplification methods for accurate malaria diagnosis on dried blood spots 11 Medical and Health Sciences 1108 Medical Microbiology","volume":"17","author":"Vincent","year":"2018","journal-title":"Malar. J."},{"key":"ref_40","first-page":"9","article-title":"Recovery of DNA for PCR amplification from blood and forensic samples using a chelating resin","volume":"98","author":"Willard","year":"1998","journal-title":"Methods Mol. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"S199","DOI":"10.1016\/S0035-9203(02)90077-5","article-title":"Alternative polymerase chain reaction method to identify Plasmodium species in human blood samples: The semi-nested multiplex malaria PCR (SnM-PCR)","volume":"96","author":"Rubio","year":"2002","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2875-11-339","article-title":"Long-term storage limits PCR-based analyses of malaria parasites in archival dried blood spots","volume":"11","author":"Hwang","year":"2012","journal-title":"Malar. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"922","DOI":"10.4269\/ajtmh.14-0602","article-title":"The effect of storage and extraction methods on amplification of plasmodium falciparum DNA from dried blood spots","volume":"92","author":"Schwartz","year":"2015","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"144","DOI":"10.4269\/ajtmh.1998.59.144","article-title":"Human occult loiasis: Improvement in diagnostic sensitivity by the use of a nested polymerase chain reaction","volume":"59","author":"Kassambara","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/S0272-2712(18)30531-6","article-title":"Laboratory diagnosis of filariasis","volume":"4","author":"Eberhard","year":"1991","journal-title":"Clin. Lab. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"565","DOI":"10.4269\/ajtmh.1995.52.565","article-title":"Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: Polymerase chain reaction methods for surveillance in Africa","volume":"52","author":"Plowe","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_47","unstructured":"Blas, I., Ru\u00edz-Zarzuela, I., and Vallejo, A. (2021, May 04). WinEpi: Working in Epidemiology. An Online Epidemiological Tool. ISVEE 11: Proceedings of the 11th Symposium of the International Society for Veterinary Epi-Demiology and Economics, Cairns (Australia). Theme 4-Tools & Training for Epidemiologists: Poste. Available online: http:\/\/winepi.net\/."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0065-308X(03)53004-4","article-title":"Diagnosis of human filariases (except onchocerciasis)","volume":"53","author":"Walther","year":"2003","journal-title":"Adv. Parasitol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"506","DOI":"10.2144\/000114018","article-title":"Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material","volume":"54","author":"Walsh","year":"2013","journal-title":"Biotechniques"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"42","DOI":"10.4269\/ajtmh.2003.69.42","article-title":"Storage duration and polymerase chain reaction detection of Plasmodium falciparum from blood spots on filter paper","volume":"69","author":"Chaorattanakawee","year":"2003","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3539","DOI":"10.1128\/JCM.00522-10","article-title":"PCR-based pooling of dried blood spots for detection of malaria parasites: Optimization and application to a cohort of Ugandan children","volume":"48","author":"Hsiang","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1017\/S0022149X15000607","article-title":"The relationship between microfilaraemic and amicrofilaraemic loiasis involving co-infection with Mansonella perstans and clinical symptoms in an exposed population from Gabon","volume":"90","author":"Kendjo","year":"2016","journal-title":"J. Helminthol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.pt.2017.12.003","article-title":"The Population Biology and Transmission Dynamics of Loa loa","volume":"34","author":"Whittaker","year":"2018","journal-title":"Trends Parasitol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1039\/C4LC01459F","article-title":"A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples","volume":"15","author":"Sun","year":"2015","journal-title":"Lab Chip"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Wanji, S., Eyong, E.E.J., Tendongfor, N., Ngwa, C.J., Esuka, E.N., Kengne-Ouafo, A.J., Datchoua-Poutcheu, F.R., Enyong, P., Agnew, D., and Eversole, R.R. (2017). Ivermectin treatment of Loa loa hyper-microfilaraemic baboons (Papio anubis): Assessment of microfilarial load reduction, haematological and biochemical parameters and histopathological changes following treatment. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005576"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s13071-020-04506-3","article-title":"Validation of Loop Mediated Isothermal Amplification for the Detection of Loa loa Infection in Chrysops sp in Experimental and Natural Field Conditions","volume":"14","author":"Amambo","year":"2020","journal-title":"Parasit. Vectors"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1111\/tmi.13117","article-title":"Field and laboratory comparative evaluation of a LAMP assay for the diagnosis of urogenital schistosomiasis in Cubal, Central Angola","volume":"23","author":"Gandasegui","year":"2018","journal-title":"Trop. Med. Int. Health"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Cevallos, W., Fern\u00e1ndez-Soto, P., Calvopi\u00f1a, M., Buend\u00eda-S\u00e1nchez, M., L\u00f3pez-Ab\u00e1n, J., Vicente, B., and Muro, A. (2018). Diagnosis of amphimeriasis by LAMPhimerus assay in human stool samples long-Term storage onto filter paper. 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