{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:01:49Z","timestamp":1760238109315,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Primary soft-tissue lymphoma (PSTL) is a rare extranodal non-Hodgkin lymphoma, characterized by a mass growing within soft-tissue, which is connective tissue, adipose tissue, and skeletal muscle. Here, we describe a case of biphenotypic lymphoblastic lymphoma arising from soft tissue of the popliteal fossa in an 11-year-old boy. A pediatric review about PSTL revealed that anaplastic large cell lymphoma is the most common histological type and a biphenotypic lymphoblastic lymphoma has not yet been reported in childhood. Lymphoma should always be considered in patients presenting with a soft-tissue mass, and a comprehensive immunohistochemical evaluation, including B-cell, T-cell, and myeloid markers, is needed to make a correct diagnosis and establish the most suitable treatment.<\/jats:p>","DOI":"10.3390\/diagnostics12071649","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T07:51:56Z","timestamp":1657180316000},"page":"1649","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extranodal Biphenotypic Non-Hodgkin Lymphoma of the Popliteal Cavity: A Case Report and Review of Literature"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7778-0525","authenticated-orcid":false,"given":"Giulia A.","family":"Restivo","sequence":"first","affiliation":[{"name":"Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties \u201cG. D\u2019Alessandro\u201d, University of Palermo, 90127 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4802-1866","authenticated-orcid":false,"given":"Lara","family":"Mussolin","sequence":"additional","affiliation":[{"name":"Clinic of Pediatric Hemato-Oncology, Department of Women\u2019s and Children\u2019s Health, University of Padova, 35128 Padova, Italy"},{"name":"Institute of Pediatric Research-Fondazione Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"D\u2019Angelo","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hemato-Oncology, ARNAS Ospedali Civico, G. Di Cristina, 90127 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7104-273X","authenticated-orcid":false,"given":"Angelica","family":"Zin","sequence":"additional","affiliation":[{"name":"Institute of Pediatric Research-Fondazione Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4793-5263","authenticated-orcid":false,"given":"Martina","family":"Pigazzi","sequence":"additional","affiliation":[{"name":"Clinic of Pediatric Hemato-Oncology, Department of Women\u2019s and Children\u2019s Health, University of Padova, 35128 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6675-867X","authenticated-orcid":false,"given":"Elisa","family":"Carraro","sequence":"additional","affiliation":[{"name":"Clinic of Pediatric Hemato-Oncology, Department of Women\u2019s and Children\u2019s Health, University of Padova, 35128 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuele S. G.","family":"D\u2019Amore","sequence":"additional","affiliation":[{"name":"Department of Pathology, San Bortolo Hospital, 36100 Vicenza, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1190-3198","authenticated-orcid":false,"given":"Marta","family":"Pillon","sequence":"additional","affiliation":[{"name":"Clinic of Pediatric Hemato-Oncology, Department of Women\u2019s and Children\u2019s Health, University of Padova, 35128 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3412-0399","authenticated-orcid":false,"given":"Piero","family":"Farruggia","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hemato-Oncology, ARNAS Ospedali Civico, G. Di Cristina, 90127 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1023\/A:1008282818705","article-title":"Primary extranodal non-Hodgkin\u2019s lymphomas. Part 1: Gastrointestinal, cutaneous and genitourinary lymphomas","volume":"8","author":"Zucca","year":"1997","journal-title":"Ann. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1023\/A:1008313229892","article-title":"Primary extranodal non-Hodgkin\u2019s lymphomas. Part 2: Head and neck, central nervous system and other less common sites","volume":"10","author":"Zucca","year":"1999","journal-title":"Ann. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1097\/00000478-198705000-00004","article-title":"Primary extranodal soft tissue lymphoma of the extremities","volume":"11","author":"Travis","year":"1987","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0046-8177(96)90065-9","article-title":"Lymphoma in soft tissue: A clinicopathologic study of 19 cases","volume":"27","author":"Salamao","year":"1996","journal-title":"Hum. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.clml.2012.10.003","article-title":"Non-hodgkin lymphomas presenting as soft tissue masses: A single center experience and meta-analysis of the published series","volume":"13","author":"Derenzini","year":"2013","journal-title":"Clin. Lymphoma Myeloma Leuk."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1038\/leu.2016.361","article-title":"NUP98-fusion transcripts characterize different biological entities within acute myeloid leukemia: A report from the AIEOP-AML group","volume":"31","author":"Bisio","year":"2017","journal-title":"Leukemia"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1182\/blood-2013-03-491621","article-title":"Results of the AIEOP AML 2002\/01 multicenter prospective trial for the treatment of children with acute myeloid leukemia","volume":"122","author":"Pession","year":"2013","journal-title":"Blood"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3860","DOI":"10.1182\/blood-2012-09-454454","article-title":"Screening of novel genetic aberrations in pediatric acute myeloid leukemia: A report from the AIEOP AML-2002 study group","volume":"120","author":"Pigazzi","year":"2012","journal-title":"Blood"},{"key":"ref_9","unstructured":"Swerdlow, S.H., Campo, E., Harris, N.L., Jaffe, E.S., Pileri, S.A., Stein, H., and Thiele, J. (2017). Acute leukemias of ambiguous lineage. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, IARC Press."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.3109\/10428194.2010.520049","article-title":"Clinicopathologic features of primary lymphoma in soft tissue","volume":"51","author":"Yang","year":"2010","journal-title":"Leuk. Lymphoma"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1097\/00043426-199123000-00016","article-title":"Childhood Ki-1 lymphoma: Presentation as a buttock mass","volume":"13","author":"Winter","year":"1991","journal-title":"Am. J. Pediatr. Hematol. Oncol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/0363-5023(93)90301-I","article-title":"Primary non-Hodgkin\u2019s lymphoma presenting in the upper extremity: A case report","volume":"18","author":"Evans","year":"1993","journal-title":"J. Hand Surg. Am."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.2214\/ajr.172.5.10227519","article-title":"Primary lymphoma of skeletal muscle","volume":"172","author":"Chew","year":"1999","journal-title":"AJR Am. J. Roentgenol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/S0165-4608(00)00285-5","article-title":"Primary CD30\/Ki-1 positive anaplastic large cell lymphoma of skeletal muscle with der(17)t(1;17)(q11;p11)","volume":"122","author":"Ishii","year":"2000","journal-title":"Cancer Genet. Cytogenet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1001\/archpedi.155.3.411","article-title":"Pathological case of the month. Ki-1-positive anaplastic large cell lymphoma involving muscle","volume":"155","author":"Menon","year":"2001","journal-title":"Arch. Pediatr. Adolesc. Med."},{"key":"ref_16","first-page":"97","article-title":"Primary CD30\/ALK-1 positive anaplastic large cell lymphoma of the skeletal muscle in a child","volume":"101","author":"Driss","year":"2009","journal-title":"Pathologica"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1080\/08880010902771531","article-title":"Anaplastic large cell lymphoma with primary involvement of skeletal muscle: A rare case report and review of the literature","volume":"26","author":"Wu","year":"2009","journal-title":"Pediatr. Hematol. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1159\/000324972","article-title":"ALK+ anaplastic large cell lymphoma with cohesive, perivascular arrangements on cytology, mimicking a soft tissue sarcoma: A report of 2 cases","volume":"54","author":"Rekhi","year":"2010","journal-title":"Acta Cytol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/1746-1596-7-38","article-title":"Case report: A unique pediatric case of a primary CD8 expressing ALK-1 positive anaplastic large cell lymphoma of skeletal muscle","volume":"7","author":"Gaiser","year":"2012","journal-title":"Diagn. Pathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1159\/000335744","article-title":"Primary anaplastic large cell lymphoma of the psoas muscle: A case report and literature review","volume":"127","author":"Kounami","year":"2012","journal-title":"Acta Haematol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"317","DOI":"10.4103\/0973-1482.113410","article-title":"Small cell variant of anaplastic large cell lymphoma presenting as arm mass in a child: A rare entity with diagnostic challenge","volume":"9","author":"Pasricha","year":"2013","journal-title":"J. Cancer Res. Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e245685","DOI":"10.1136\/bcr-2021-245685","article-title":"ALK-positive anaplastic large cell lymphoma of skeletal muscle masquerading as soft tissue sarcoma","volume":"15","author":"Gupta","year":"2022","journal-title":"BMJ Case Rep."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Mussolin, L., Le Deley, M.C., Carraro, E., Damm-Welk, C., Attarbaschi, A., Williams, D., Burke, A., Horibe, K., Nakazawa, A., and Wrobel, G. (2020). Prognostic Factors in Childhood Anaplastic Large Cell Lymphoma: Long Term Results of the International ALCL99 Trial. Cancers, 12.","DOI":"10.3390\/cancers12102747"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1002\/1097-0142(19920215)69:4<1067::AID-CNCR2820690439>3.0.CO;2-O","article-title":"Fine-needle aspiration biopsy of small round blue cell tumors of childhood","volume":"69","author":"McGahey","year":"1992","journal-title":"Cancer"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1097\/00000478-199807000-00002","article-title":"Precursor B-Lymphoblastic lymphoma presenting as a solitary bone tumor and mimicking Ewing\u2019s sarcoma: A report of four cases and review of the literature","volume":"22","author":"Ozdemirli","year":"1998","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1097\/00000478-200012000-00010","article-title":"Immunohistochemical detection of FLI-1 protein expression: A study of 132 round cell tumors with emphasis on CD99-positive mimics of Ewing\u2019s sarcoma\/primitive neuroectodermal tumor","volume":"24","author":"Folpe","year":"2000","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.acthis.2015.03.011","article-title":"Immunohistochemistry as potential diagnostic pitfall in the most common solid tumors of children and adolescents","volume":"117","author":"Magro","year":"2015","journal-title":"Acta Histochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1038\/modpathol.3880455","article-title":"Differentiating lymphoblastic lymphoma and Ewing\u2019s sarcoma: Lymphocyte markers and gene rearrangement","volume":"14","author":"Ozdemirli","year":"2001","journal-title":"Mod. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1309\/K1XJ-6CXR-BQQU-V255","article-title":"Ewing sarcoma vs lymphoblastic lymphoma. A comparative immunohistochemical study","volume":"115","author":"Lucas","year":"2001","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1111\/bjh.15793","article-title":"Lymphoblastic lymphoma in children and adolescents: Review of current challenges and future opportunities","volume":"185","author":"Burkhardt","year":"2019","journal-title":"Br. J. Haematol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1097\/PAS.0b013e318213e90e","article-title":"Diagnosis and immunophenotype of 188 pediatric lymphoblastic lymphomas treated within a randomized prospective trial: Experiences and preliminary recommendations from the European childhood lymphoma pathology panel","volume":"35","author":"Oschlies","year":"2011","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.3324\/haematol.2009.009282","article-title":"Clinical characteristics and outcome of children with biphenotypic acute leukemia","volume":"94","author":"Owaidah","year":"2009","journal-title":"Haematologica"}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/12\/7\/1649\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:43:45Z","timestamp":1760139825000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/12\/7\/1649"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,7]]},"references-count":32,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["diagnostics12071649"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics12071649","relation":{},"ISSN":["2075-4418"],"issn-type":[{"type":"electronic","value":"2075-4418"}],"subject":[],"published":{"date-parts":[[2022,7,7]]}}}