{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:51:52Z","timestamp":1784544712651,"version":"3.55.0"},"reference-count":33,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nazarbayev University: Nazarbayev University School of Medicine funding for Students\u2019 Master Projects (Molecular Medicine)","award":["240919FD3912"],"award-info":[{"award-number":["240919FD3912"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Basophils are the least abundant circulating leukocytes, and their immunological role has not yet been completely elucidated. There is evidence supporting their immunomodulatory role in several pathological settings; recently, studies in both experimental models and humans suggested that basophil homeostasis may be altered in systemic lupus erythematosus (SLE). Here, we first assessed circulating basophils in children affected with pediatric SLE (pSLE). In this cross-sectional study, circulating basophils were enumerated by fluorescence-based flow cytometry analysis in children affected with pSLE, in addition to children suffering from juvenile idiopathic arthritis (JIA) or non-inflammatory\/non-rheumatic conditions. This study included 52 pediatric patients distributed in these three groups. We observed a statistically significant reduction of peripherally circulating basophils in children with pSLE compared to the other two groups of patients. This preliminary study is consistent with the available studies in adult patients with SLE showing a reduced number of circulating basophils. However, further research is needed to draw final conclusions on basophils\u2019 homeostasis in pSLE, in addition to their correlation with the disease activity and concomitant therapies.<\/jats:p>","DOI":"10.3390\/diagnostics12071701","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T20:52:41Z","timestamp":1657659161000},"page":"1701","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Analysis of Peripheral Blood Basophils in Pediatric Systemic Lupus Erythematosus"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1834-1909","authenticated-orcid":false,"given":"Kuanysh","family":"Dossybayeva","sequence":"first","affiliation":[{"name":"Department of Medicine, Nazarbayev University School of Medicine, Nur-Sultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yergali","family":"Bexeitov","sequence":"additional","affiliation":[{"name":"Department of Medicine, Nazarbayev University School of Medicine, Nur-Sultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaure","family":"Mukusheva","sequence":"additional","affiliation":[{"name":"Program of Pediatric Rheumatology, Clinical Academic Department of Pediatrics, National Research Center for Maternal and Child Health, University Medical Center, Nur-Sultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaina","family":"Almukhamedova","sequence":"additional","affiliation":[{"name":"Program of Pediatric Rheumatology, Clinical Academic Department of Pediatrics, National Research Center for Maternal and Child Health, University Medical Center, Nur-Sultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maykesh","family":"Assylbekova","sequence":"additional","affiliation":[{"name":"Program of Pediatric Rheumatology, Clinical Academic Department of Pediatrics, National Research Center for Maternal and Child Health, University Medical Center, Nur-Sultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diyora","family":"Abdukhakimova","sequence":"additional","affiliation":[{"name":"Department of Medicine, Nazarbayev University School of Medicine, Nur-Sultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzhan","family":"Rakhimzhanova","sequence":"additional","affiliation":[{"name":"Program of Pediatric Endocrinology and Inherited Diseases, Clinical Academic Department of Pediatrics, National Research Center for Maternal and Child Health, University Medical Center, Nur-Sultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6431-9334","authenticated-orcid":false,"given":"Dimitri","family":"Poddighe","sequence":"additional","affiliation":[{"name":"Department of Medicine, Nazarbayev University School of Medicine, Nur-Sultan 010000, Kazakhstan"},{"name":"Clinical Academic Department of Pediatrics, National Research Center for Maternal and Child Health, University Medical Center, Nur-Sultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4028","DOI":"10.1182\/blood.V96.13.4028","article-title":"The human basophil: A new appreciation of its role in immune responses","volume":"96","author":"Falcone","year":"2000","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-4939-1173-8_1","article-title":"Paradigm shifts in mast cell and basophil biology and function: An emerging view of immune regulation in health and disease","volume":"1192","author":"Olivera","year":"2014","journal-title":"Methods Mol. 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