{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:08:51Z","timestamp":1790690931604,"version":"4.1.0"},"reference-count":82,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T00:00:00Z","timestamp":1659830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: PGT-based NGS revolutionized the field of reproductive medicine, becoming an integrated component within current assisted reproductive technology (ART) protocols. Methods: We searched the literature published in the last half a decade in four databases (PubMed\/Medline, ISI Web of Knowledge, ScienceDirect, and Scopus) between 2018 and 2022. Results: A total of 1388 articles were filtered, from which 60 met, initially, the eligibility criteria, but only 42 were included (\u2265100 patients\/couples\u201462,465 patients and 6628 couples in total) in the present mini-review. In total, forty-two (70.0%) reported reproductive outcomes, while eighteen (30.0%) had distinct objectives. Furthermore, n = 1, 1.66% of the studies focused on PGT, n = 1, 1.66% on pre-implantation genetic testing for monogenic disorders (PGT-M), n = 3, 5.0% on pre-implantation genetic testing for structural rearrangements (PGT-SR) and n = 55, 91.66% on pre-implantation genetic testing for aneuploidies (PGT-A). Conclusions: PGT using NGS proved to be an excellent companion that folds within the current ascending tendency among couples that require specialty care. We strongly encourage future studies to provide a systematic overview expanded at a larger scale on the role of the PGT-NGS.<\/jats:p>","DOI":"10.3390\/diagnostics12081911","type":"journal-article","created":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T21:03:50Z","timestamp":1659906230000},"page":"1911","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["A Mini-Review Regarding the Clinical Outcomes of In Vitro Fertilization (IVF) Following Pre-Implantation Genetic Testing (PGT)-Next Generation Sequencing (NGS) Approach"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6618-141X","authenticated-orcid":false,"given":"Bogdan","family":"Doroftei","sequence":"first","affiliation":[{"name":"Faculty of Medicine, University of Medicine and Pharmacy \u201cGrigore T. Popa\u201d, University Street, no 16, 700115 Iasi, Romania"},{"name":"Clinical Hospital of Obstetrics and Gynecology \u201cCuza Voda\u201d, Cuza Voda Street, no 34, 700038 Iasi, Romania"},{"name":"Origyn Fertility Center, Palace Street, no 3C, 700032 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4023-1765","authenticated-orcid":false,"given":"Ovidiu-Dumitru","family":"Ilie","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Biology, \u201cAlexandru Ioan Cuza\u201d University, Carol I Avenue, no 20A, 700505 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4987-5049","authenticated-orcid":false,"given":"Nicoleta","family":"Anton","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Medicine and Pharmacy \u201cGrigore T. Popa\u201d, University Street, no 16, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodora","family":"Armeanu","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Medicine and Pharmacy \u201cGrigore T. Popa\u201d, University Street, no 16, 700115 Iasi, Romania"},{"name":"Clinical Hospital of Obstetrics and Gynecology \u201cCuza Voda\u201d, Cuza Voda Street, no 34, 700038 Iasi, Romania"},{"name":"Origyn Fertility Center, Palace Street, no 3C, 700032 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciprian","family":"Ilea","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Medicine and Pharmacy \u201cGrigore T. Popa\u201d, University Street, no 16, 700115 Iasi, Romania"},{"name":"Clinical Hospital of Obstetrics and Gynecology \u201cCuza Voda\u201d, Cuza Voda Street, no 34, 700038 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1093\/humupd\/8.4.333","article-title":"Conception to ongoing pregnancy: The \u2018black box\u2019 of early pregnancy loss","volume":"8","author":"Macklon","year":"2002","journal-title":"Hum. Reprod. Update"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"De Rycke, M., and Berckmoes, V. (2020). Preimplantation Genetic Testing for Monogenic Disorders. Genes, 11.","DOI":"10.3390\/genes11080871"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/344768a0","article-title":"Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification","volume":"344","author":"Handyside","year":"1990","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"F29","DOI":"10.1530\/REP-17-0683","article-title":"Chromosomal analysis in IVF: Just how useful is it?","volume":"156","author":"Griffin","year":"2018","journal-title":"Reproduction"},{"key":"ref_5","first-page":"1786","article-title":"The International Glossary on Infertility and Fertility Care, 2017 \u2020,\u2021,\u00a7","volume":"32","author":"Adamson","year":"2017","journal-title":"Hum. Reprod."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"ESHRE PGT Consortium Steering Committee, Carvalho, F., Coonen, E., Goossens, V., Kokkali, G., Rubio, C., Meijer-Hoogeveen, M., Moutou, C., Vermeulen, N., and De Rycke, M. (2020). ESHRE PGT Consortium good practice recommendations for the organisation of PGT \u2020. Hum. Reprod. Open, 2020, hoaa021.","DOI":"10.1093\/hropen\/hoaa021"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"ESHRE PGT Consortium and SIG-Embryology Biopsy Working Group, Kokkali, G., Coticchio, G., Bronet, F., Celebi, C., Cimadomo, D., Goossens, V., Liss, J., Nunes, S., and Sfontouris, I. (2020). ESHRE PGT Consortium and SIG Embryology good practice recommendations for polar body and embryo biopsy for PGT \u2020. Hum. Reprod. Open, 2020, hoaa020.","DOI":"10.1093\/hropen\/hoaa020"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"ESHRE PGT-M Working Group, Carvalho, F., Moutou, C., Dimitriadou, E., Dreesen, J., Gim\u00e9nez, C., Goossens, V., Kakourou, G., Vermeulen, N., and Zuccarello, D. (2020). ESHRE PGT Consortium good practice recommendations for the detection of monogenic disorders \u2020. Hum. Reprod. Open, 2020, hoaa018.","DOI":"10.1093\/hropen\/hoaa018"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"ESHRE PGT-SR\/PGT-A Working Group, Coonen, E., Rubio, C., Christopikou, D., Dimitriadou, E., Gontar, J., Goossens, V., Maurer, M., Spinella, F., and Vermeulen, N. (2020). ESHRE PGT Consortium good practice recommendations for the detection of structural and numerical chromosomal aberrations. Hum. Reprod. Open, 2020, hoaa017.","DOI":"10.1093\/hropen\/hoaa017"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0899-3467(07)60142-6","article-title":"Writing narrative literature reviews for peer-reviewed journals: Secrets of the trade","volume":"5","author":"Green","year":"2006","journal-title":"J. Chiropr. Med."},{"key":"ref_11","first-page":"77","article-title":"Blastocyst contractions are strongly related with aneuploidy, lower implantation rates, and slow-cleaving embryos: A time lapse study","volume":"24","author":"Gazzo","year":"2020","journal-title":"JBRA Assist. Reprod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/s10815-020-02050-8","article-title":"Does blastocyst mitochondrial DNA content affect miscarriage rate in patients undergoing single euploid frozen embryo transfer?","volume":"38","author":"Elkhatib","year":"2021","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.fertnstert.2018.08.057","article-title":"Transfer of embryos with segmental mosaicism is associated with a significant reduction in live-birth rate","volume":"111","author":"Zore","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1007\/s10815-020-02045-5","article-title":"Suboptimal trophectoderm mitochondrial DNA level is associated with delayed blastocyst development","volume":"38","author":"Wu","year":"2021","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.rbmo.2021.06.022","article-title":"Experience analysing over 190,000 embryo trophectoderm biopsies using a novel FAST-SeqS preimplantation genetic testing assay","volume":"44","author":"Neitzel","year":"2022","journal-title":"Reprod. Biomed. Online"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.rbmo.2018.12.043","article-title":"Evaluation of preimplantation genetic testing based on next-generation sequencing for balanced reciprocal translocation carriers","volume":"38","author":"Cai","year":"2019","journal-title":"Reprod. Biomed. Online"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.fertnstert.2020.04.003","article-title":"Preimplantation genetic testing for aneuploidy in patients with partial X monosomy using their own oocytes: Is this a suitable indication?","volume":"114","author":"Giles","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.fertnstert.2020.09.140","article-title":"Routine endometrial receptivity array in first embryo transfer cycles does not improve live birth rate","volume":"115","author":"Riestenberg","year":"2021","journal-title":"Fertil. Steril."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.rbmo.2021.11.010","article-title":"Do clinical outcomes differ for day-5 versus day-6 single embryo transfers controlled for endometrial factor?","volume":"44","author":"Stankewicz","year":"2022","journal-title":"Reprod. Biomed. Online"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejogrb.2019.01.007","article-title":"Impact of in vitro fertilization-preimplantation genetic testing (IVF-PGT) funding policy on clinical outcome: An issue that stems beyond effectiveness of treatment","volume":"235","author":"Shao","year":"2019","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1016\/j.fertnstert.2019.01.017","article-title":"Role of aneuploidy screening in preimplantation genetic testing for monogenic diseases in young women","volume":"111","author":"Hou","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"287.e1","DOI":"10.1016\/j.ajog.2021.03.026","article-title":"The association between prior cesarean delivery and subsequent in vitro fertilization outcomes in women undergoing autologous, frozen-thawed single euploid embryo transfer","volume":"225","author":"Friedenthal","year":"2021","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1016\/j.fertnstert.2020.10.051","article-title":"Diminished ovarian reserve is associated with reduced euploid rates via preimplantation genetic testing for aneuploidy independently from age: Evidence for concomitant reduction in oocyte quality with quantity","volume":"115","author":"Jaswa","year":"2021","journal-title":"Fertil. Steril."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"hoy023","DOI":"10.1093\/hropen\/hoy023","article-title":"Comprehensive mitochondrial DNA analysis and IVF outcome","volume":"2018","author":"Lledo","year":"2018","journal-title":"Hum. Reprod. Open"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.fertnstert.2019.03.041","article-title":"Retrospective analysis of meiotic segregation pattern and interchromosomal effects in blastocysts from inversion preimplantation genetic testing cycles","volume":"112","author":"Xie","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1007\/s10815-020-02053-5","article-title":"Evaluation of chromosomal abnormalities from preimplantation genetic testing to the reproductive outcomes: A comparison between three different structural rearrangements based on next-generation sequencing","volume":"38","author":"Yuan","year":"2021","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.gocm.2021.10.003","article-title":"The interaction effect between advanced paternal age and paternal obesity is associated with the low implantation rate in couples with unexplained recurrent pregnancy loss","volume":"1","author":"Li","year":"2021","journal-title":"Gynecol. Obstet. Clin. Med."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Niu, W., Wang, L., Xu, J., Li, Y., Shi, H., Li, G., Jin, H., Song, W., Wang, F., and Sun, Y. (2020). Improved clinical outcomes of preimplantation genetic testing for aneuploidy using MALBAC-NGS compared with MDA-SNP array. BMC Pregnancy Childbirth, 20.","DOI":"10.1186\/s12884-020-03082-9"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1007\/s10815-019-01496-9","article-title":"Euploid blastocysts implant irrespective of their morphology after NGS-(PGT-A) testing in advanced maternal age patients","volume":"36","author":"Odia","year":"2019","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2238","DOI":"10.1016\/j.ajhg.2021.11.002","article-title":"Mosaic human preimplantation embryos and their developmental potential in a prospective, non-selection clinical trial","volume":"108","author":"Capalbo","year":"2021","journal-title":"Am. J. Hum. Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.rbmo.2021.04.006","article-title":"Complex mosaic blastocysts after preimplantation genetic testing: Prevalence and outcomes after re-biopsy and re-vitrification","volume":"43","author":"Zhou","year":"2021","journal-title":"Reprod. Biomed. Online"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1007\/s10815-021-02137-w","article-title":"Trophectoderm biopsy protocols may impact the rate of mosaic blastocysts in cycles with pre-implantation genetic testing for aneuploidy","volume":"38","author":"Xiong","year":"2021","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Lin, P.-Y., Lee, C.-I., Cheng, E.-H., Huang, C.-C., Lee, T.-H., Shih, H.-H., Pai, Y.-P., Chen, Y.-C., and Lee, M.-S. (2020). Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter?. J. Clin. Med., 9.","DOI":"10.3390\/jcm9061695"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.rbmo.2019.05.020","article-title":"Embryo morphokinetics is potentially associated with clinical outcomes of single-embryo transfers in preimplantation genetic testing for aneuploidy cycles","volume":"39","author":"Lee","year":"2019","journal-title":"Reprod. Biomed. Online"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1080\/14647273.2018.1551628","article-title":"High implantation and clinical pregnancy rates with single vitrified-warmed blastocyst transfer and optional aneuploidy testing for all patients","volume":"23","author":"Gorodeckaja","year":"2020","journal-title":"Hum. Fertil."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.rbmo.2019.05.011","article-title":"Miscarriage history association with euploid embryo transfer outcomes","volume":"39","author":"Wang","year":"2019","journal-title":"Reprod. Biomed. Online"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.rbmo.2021.02.005","article-title":"Comparison of single euploid blastocyst transfer cycle outcome derived from embryos with normal or abnormal cleavage patterns","volume":"42","author":"Ozbek","year":"2021","journal-title":"Reprod. Biomed. Online"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/j.fertnstert.2019.07.1346","article-title":"Preimplantation genetic testing for aneuploidy versus morphology as selection criteria for single frozen-thawed embryo transfer in good-prognosis patients: A multicenter randomized clinical trial","volume":"112","author":"Kaplan","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.fertnstert.2018.03.026","article-title":"Pregnancy outcomes from more than 1800 in vitro fertilization cycles with the use of 24-chromosome single-nucleotide polymorphism-based preimplantation genetic testing for aneuploidy","volume":"110","author":"Simon","year":"2018","journal-title":"Fertil. Steril."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/j.rbmo.2021.03.004","article-title":"Which factors affect the likelihood of miscarriage after single euploid blastocyst transfer?","volume":"42","author":"Boynukalin","year":"2021","journal-title":"Reprod. Biomed. Online"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.jmoldx.2021.02.011","article-title":"Next-Generation Sequencing Is More Efficient at Detecting Mosaic Embryos and Improving Pregnancy Outcomes than Single-Nucleotide Polymorphism Array Analysis","volume":"23","author":"Xiao","year":"2021","journal-title":"J. Mol. Diagn."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.fertnstert.2020.07.052","article-title":"A multicenter, prospective, blinded, nonselection study evaluating the predictive value of an aneuploid diagnosis using a targeted next-generation sequencing-based preimplantation genetic testing for aneuploidy assay and impact of biopsy","volume":"115","author":"Tiegs","year":"2021","journal-title":"Fertil. Steril."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e14065","DOI":"10.1111\/and.14065","article-title":"Comparison of preimplantation genetic testing for aneuploidy versus intracytoplasmic sperm injection in severe male infertility","volume":"53","author":"Xu","year":"2021","journal-title":"Andrologia"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.fertnstert.2019.11.016","article-title":"Higher chromosomal abnormality rate in blastocysts from young patients with idiopathic recurrent pregnancy loss","volume":"113","author":"Liu","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1007\/s43032-021-00519-0","article-title":"Next-Generation Sequencing (NGS)-Based Preimplantation Genetic Testing for Aneuploidy (PGT-A) of Trophectoderm Biopsy for Recurrent Implantation Failure (RIF) Patients: A Retrospective Study","volume":"28","author":"Tong","year":"2021","journal-title":"Reprod. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/j.fertnstert.2020.09.139","article-title":"Female obesity increases the risk of miscarriage of euploid embryos","volume":"115","author":"Cozzolino","year":"2021","journal-title":"Fertil. Steril."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.rbmo.2021.06.008","article-title":"Effect of age and morphology on sustained implantation rate after euploid blastocyst transfer","volume":"43","author":"Awadalla","year":"2021","journal-title":"Reprod. Biomed. Online"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.rbmo.2018.10.007","article-title":"The correlation between morphology and implantation of euploid human blastocysts","volume":"38","author":"Nazem","year":"2019","journal-title":"Reprod. Biomed. Online"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.rbmo.2021.09.027","article-title":"Clinical, obstetric and perinatal outcomes after vitrified-warmed euploid blastocyst transfer are independent of cryo-storage duration","volume":"44","author":"Cimadomo","year":"2022","journal-title":"Reprod. Biomed. Online"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"103852","DOI":"10.1016\/j.ejmg.2020.103852","article-title":"Clinical error rates of next generation sequencing and array comparative genomic hybridization with single thawed euploid embryo transfer","volume":"63","author":"Friedenthal","year":"2020","journal-title":"Eur. J. Med. Genet."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/j.fertnstert.2019.02.024","article-title":"Endometrial preparation before the transfer of single, vitrified-warmed, euploid blastocysts: Does the duration of estradiol treatment influence clinical outcome?","volume":"111","author":"Sekhon","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.tjog.2020.11.034","article-title":"Comprehensive assessment of a clinic\u2019s experience of preimplantation genetic testing by a cumulative rate","volume":"60","author":"Li","year":"2021","journal-title":"Taiwan. J. Obstet. Gynecol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.rbmo.2021.09.007","article-title":"Effect of parental origin and predictors for obtaining a euploid embryo in balanced translocation carriers","volume":"44","author":"Tong","year":"2022","journal-title":"Reprod. Biomed. Online"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.rbmo.2018.08.006","article-title":"Prevalence and authenticity of de-novo segmental aneuploidy (>16 Mb) in human blastocysts as detected by next-generation sequencing","volume":"37","author":"Zhou","year":"2018","journal-title":"Reprod. Biomed. Online"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1007\/s10815-022-02413-3","article-title":"Preimplantation genetic testing for aneuploidy (PGT-A)\u2014A single-center experience","volume":"39","author":"Nair","year":"2022","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tjog.2020.05.005","article-title":"Could PGT-A pick up true abnormalities that have clinical relevance? Retrospective analysis of 1043 embryos","volume":"59","author":"Liu","year":"2020","journal-title":"Taiwan. J. Obstet. Gynecol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.fertnstert.2019.03.040","article-title":"Euploidy rates between cycles triggered with gonadotropin-releasing hormone agonist and human chorionic gonadotropin","volume":"112","author":"Thorne","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.fertnstert.2021.02.029","article-title":"Body mass index is not associated with embryo ploidy in patients undergoing in vitro fertilization with preimplantation genetic testing","volume":"116","author":"Stovezky","year":"2021","journal-title":"Fertil. Steril."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.fertnstert.2017.09.022","article-title":"Variables associated with mitochondrial copy number in human blastocysts: What can we learn from trophectoderm biopsies?","volume":"109","author":"Mifsud","year":"2018","journal-title":"Fertil. Steril."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/j.fertnstert.2020.09.164","article-title":"Shorter telomere length of white blood cells is associated with higher rates of aneuploidy among infertile women undergoing in vitro fertilization","volume":"115","author":"Hanson","year":"2021","journal-title":"Fertil. Steril."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1080\/19396368.2020.1850908","article-title":"Effect of ovarian stimulation on embryo aneuploidy and mosaicism rate","volume":"67","author":"Cascales","year":"2021","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.fertnstert.2020.03.034","article-title":"Is there a correlation between paternal age and aneuploidy rate? An analysis of 3118 embryos derived from young egg donors","volume":"114","author":"Dviri","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.repbio.2018.10.002","article-title":"The relationship between preimplantation morphokinetics of human embryos and sex chromosome pattern","volume":"18","author":"Zeyad","year":"2018","journal-title":"Reprod. Biol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"751.e1","DOI":"10.1016\/j.ajog.2020.04.035","article-title":"Multicenter prospective study of concordance between embryonic cell-free DNA and trophectoderm biopsies from 1301 human blastocysts","volume":"223","author":"Rubio","year":"2020","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s00404-021-06050-6","article-title":"A mathematical model for predicting the number of transferable blastocysts in next-generation sequencing-based preimplantation genetic testing","volume":"305","author":"Cai","year":"2022","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/rmb2.12352","article-title":"Pre-implantation genetic testing: Past, present, future","volume":"20","author":"Takeuchi","year":"2020","journal-title":"Reprod. Med. Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1007\/s10815-020-01694-w","article-title":"Euploid rates among oocyte donors: Is there an optimal age for donation?","volume":"37","author":"Hoyos","year":"2020","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Roos Kulmann, M.I., Lumertz Martello, C., Bos-Mikich, A., and Frantz, N. (2020). Pronuclear and blastocyst morphology are associated age-dependently with embryo ploidy in in vitro fertilization cycles. Hum. Fertil., 369\u2013379.","DOI":"10.1080\/14647273.2020.1808716"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.rbmo.2021.01.001","article-title":"Euploidy rates of embryos in young patients with good and low prognosis according to the POSEIDON criteria","volume":"42","author":"Boynukalin","year":"2021","journal-title":"Reprod. Biomed. Online"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.rbmo.2022.01.001","article-title":"Blastocyst transfer increases cumulative-live-birth-rates and reduces time and cost to livebirth compared with cleavage stage in recipients of donated oocytes. A randomized controlled trial","volume":"44","author":"Clua","year":"2022","journal-title":"Reprod. Biomed. Online"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.fertnstert.2019.03.006","article-title":"Neonatal outcomes of live births after blastocyst biopsy in preimplantation genetic testing cycles: A follow-up of 1721 children","volume":"112","author":"He","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"378","DOI":"10.3390\/biology2010378","article-title":"Next-generation sequencing: From understanding biology to personalized medicine","volume":"2","author":"Frese","year":"2013","journal-title":"Biology"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"251364","DOI":"10.1155\/2012\/251364","article-title":"Comparison of Next-Generation Sequencing Systems","volume":"2012","author":"Liu","year":"2012","journal-title":"J. Biomed. Biotechnol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1146\/annurev-anchem-062012-092628","article-title":"Next-Generation Sequencing Platforms","volume":"6","author":"Mardis","year":"2013","journal-title":"Annu. Rev. Anal. Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1146\/annurev.genom.9.081307.164359","article-title":"Next-Generation DNA Sequencing Methods","volume":"9","author":"Mardis","year":"2008","journal-title":"Annu. Rev. Genomics Hum. Genet."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Gupta, A., and Gupta, U.D. (2020). Next generation sequencing and its applications. Animal Biotechnology, Academic Press.","DOI":"10.1016\/B978-0-12-811710-1.00018-5"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nrn3271","article-title":"Use of next-generation sequencing and other whole-genome strategies to dissect neurological disease","volume":"13","author":"Bras","year":"2012","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/gm345","article-title":"Non-invasive prenatal diagnosis of aneuploidies: New technologies and clinical applications","volume":"4","author":"Papageorgiou","year":"2012","journal-title":"Genome Med."},{"key":"ref_79","first-page":"48","article-title":"Noninvasive prenatal testing: The future is now","volume":"6","author":"Norwitz","year":"2013","journal-title":"Rev. Obstet. Gynecol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Demkow, U., and P\u0142oski, R. (2016). Next Generation Sequencing as a Tool for Noninvasive Prenatal Tests. Clinical Applications for Next-Generation Sequencing, Academic Press.","DOI":"10.1016\/B978-0-12-801739-5.00011-8"},{"key":"ref_81","first-page":"4","article-title":"Next-generation sequencing approach for the diagnosis of human diseases: Open challenges and new opportunities","volume":"29","author":"Galbiati","year":"2018","journal-title":"Electron. J. IFCC (eJIFCC)"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Demkow, U., and P\u0142oski, R. (2016). Next Generation Sequencing\u2014General Information about the Technology, Possibilities, and Limitations. Clinical Applications for Next-Generation Sequencing, Academic Press.","DOI":"10.1016\/B978-0-12-801739-5.00011-8"}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/12\/8\/1911\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:05:25Z","timestamp":1760141125000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/12\/8\/1911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,7]]},"references-count":82,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["diagnostics12081911"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics12081911","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,7]]}}}