{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T22:23:33Z","timestamp":1780611813708,"version":"3.54.1"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,20]],"date-time":"2022-08-20T00:00:00Z","timestamp":1660953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"India\u2019s World Health Organization country office","award":["2018\/851237"],"award-info":[{"award-number":["2018\/851237"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Northeastern states of India share international borders with Myanmar, China, Bangladesh, and Bhutan, contributing 7.45% of the overall malaria cases in the country. Mizoram accounts for the highest malaria burden in the northeastern states, with perennial transmission in the hilly and deep-forested areas. Plasmodium falciparum (93%) is the most prevalent human Plasmodium species, followed by P. vivax; however, information on P. ovale and P. malariae is negligible. Rapid diagnostic tests (RDTs) are the most preferred malaria diagnostic tool followed by microscopy in this high malaria-endemic region. The present epidemiological study was carried out in July and August 2019 to assess the malaria burden in and around the Chawngte primary health center, Lawngtlai District of Mizoram, using RDTs and microscopy as diagnostic tools. World Health Organization-certified level I microscopists examined the blood smears. Diagnosis using RDTs resulted in 151 malaria cases (P. falciparum: 136; P. vivax: 15) out of 948 screened fever cases. However, blood smear examination detected 179 cases (P. falciparum: 154; P. vivax: 17; mixed P. falciparum + P. vivax infection: 3; P. malariae: 5). Analysis revealed that the risk of malaria infection was higher in the \u22655-year-old subjects than in the under-5 age group. The mean parasite density of P. malariae (1455.00\/\u03bcL blood) was the lowest; cf. with P. falciparum: 12,275.08\/\u03bcL blood. Surveillance at the point-of-care level using microscopy was able to detect all the four human Plasmodium species and their mixed infections, including P. malariae, which were missed with RDTs. Thus, the quality of microscopy along with trained manpower should be strengthened to diagnose all human malaria parasite species (particularly P. malariae and P. ovale) until the molecular tools are deployed at the field level to achieve malaria elimination by 2030.<\/jats:p>","DOI":"10.3390\/diagnostics12082015","type":"journal-article","created":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T21:05:51Z","timestamp":1661115951000},"page":"2015","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Plasmodium malariae Detected by Microscopy in the International Bordering Area of Mizoram, a Northeastern State of India"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4329-6940","authenticated-orcid":false,"given":"Kuldeep","family":"Singh","sequence":"first","affiliation":[{"name":"ICMR\u2014National Institute of Malaria Research, Field Station, Guwahati 781005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen Kumar","family":"Bharti","sequence":"additional","affiliation":[{"name":"ICMR\u2014National Institute of Malaria Research, New Delhi 110077, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naorem Chaoba","family":"Devi","sequence":"additional","affiliation":[{"name":"ICMR\u2014National Institute of Malaria Research, Field Station, Guwahati 781005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naseem","family":"Ahmed","sequence":"additional","affiliation":[{"name":"ICMR\u2014National Institute of Malaria Research, New Delhi 110077, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Sharma","sequence":"additional","affiliation":[{"name":"ICMR\u2014National Institute of Malaria Research, New Delhi 110077, India"},{"name":"International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,20]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2021). 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