{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:45:18Z","timestamp":1791852318786,"version":"4.3.4"},"reference-count":28,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T00:00:00Z","timestamp":1666656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chulalongkorn University","award":["CU_GR_63_117_30_24"],"award-info":[{"award-number":["CU_GR_63_117_30_24"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Stool samples typically contain PCR inhibitors; however, helminths are difficult to lyse and can cause false-negative PCR results. We assessed the effective methods for extracting DNA from different kinds of intestinal parasites. We compared the most common DNA extraction methods from stool samples, including the phenol-chloroform technique with or without a bead-beating step (P and PB), a QIAamp Fast DNA Stool Mini Kit (Q), and a QIAamp PowerFecal Pro DNA Kit (QB). Genomic DNA was extracted from 85 stool samples collected from patients infected with Blastocystis sp., Ascaris lumbricoides, Trichuris trichiura, hookworm, and Strongyloides stercoralis. DNA quantity and DNA quality were evaluated via spectrophotometry, and DNA integrity was assessed by PCR. We found that P and PB provided higher DNA yields (~4 times) than when using Q and QB. However, P showed the lowest detection rate of PCR (8.2%), wherein only S. stercoralis (7 out of 20 samples) was detected. QB showed the highest detection rate of PCR (61.2%). After plasmid spikes, only 5 samples by QB were negative while 60 samples by P were still negative. Remarkably, QB could extract DNA from all the groups of parasites that we tested. These results indicate that QB is the most effective DNA extraction method for the diagnosis and monitoring of intestinal parasites via PCR.<\/jats:p>","DOI":"10.3390\/diagnostics12112588","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T12:29:45Z","timestamp":1666700985000},"page":"2588","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Comparative Study of DNA Extraction Methods for the PCR Detection of Intestinal Parasites in Human Stool Samples"],"prefix":"10.3390","volume":"12","author":[{"given":"Siriporn","family":"Srirungruang","sequence":"first","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center (Chula-MRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Department of Parasitology, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buraya","family":"Mahajindawong","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center (Chula-MRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Division of Dermatology, Department of Internal Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7142-5706","authenticated-orcid":false,"given":"Panachai","family":"Nimitpanya","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center (Chula-MRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uthaitip","family":"Bunkasem","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center (Chula-MRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2937-7077","authenticated-orcid":false,"given":"Pattama","family":"Ayuyoe","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center (Chula-MRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Department of Parasitology, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surang","family":"Nuchprayoon","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center (Chula-MRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4257-1656","authenticated-orcid":false,"given":"Vivornpun","family":"Sanprasert","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center (Chula-MRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,25]]},"reference":[{"key":"ref_1","first-page":"1123","article-title":"Prevalence of intestinal protozoan infections among children in Thailand: A large-scale screening and comparative study of three standard detection methods","volume":"47","author":"Sanprasert","year":"2016","journal-title":"Southeast Asian J. 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