{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T19:43:51Z","timestamp":1776714231381,"version":"3.51.2"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Mintek"},{"name":"Department of Science and Innovation (DSI)\u2014South Africa"},{"name":"DSI\/Mintek Nanotechnology Innovation Centre (NIC)"},{"name":"University of Johannesburg (UJ)"},{"name":"University of Venda"},{"name":"Council for Scientific and Industrial Research (CSIR)\u2014South Africa"},{"name":"The National Intellectual Property Management Office (NIPMO)\u2014South Africa"},{"name":"Centre for High-Performance Computing (CHPC)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Rift Valley fever (RVF) is a mosquito-borne zoonotic disease that is caused by the Rift Valley fever virus (RVFV); Bunyaviridae: Phlebovirus. RVF disease can affect several different species, including ruminants, camels and humans and thus present a dual threat to public health and livestock food production in endemic regions. In livestock, the RVFV infection is characterised by an acute hepatitis, abortion and high mortality rates in new-born animals. The current RVF diagnostic techniques have shown good sensitivity. However, they require extensive sample processing and complex instrumentation. Owing to speed, low cost, ease of use, and most importantly, the ability to diagnose diseases at sites where they are managed, lateral flow immunoassays (LFIA) are the most widely used point-of-care (POC) tools for disease diagnosis. In this study, a lateral flow assay (LFA) device that is able to detect antibodies against RVFV, with a minimum detectable concentration of 0.125 mg\/mL, was successfully developed. The LFA also successfully detected RVFV antibodies in reference RVFV sera. Protein A (ProA), which has the ability to bind immunoglobulins from different species, was used in the detection probe, giving the developed RVFV LFA potential for multi-species diagnosis.<\/jats:p>","DOI":"10.3390\/diagnostics12112664","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T03:11:21Z","timestamp":1667445081000},"page":"2664","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies"],"prefix":"10.3390","volume":"12","author":[{"given":"Thulaganyo","family":"Domfe","sequence":"first","affiliation":[{"name":"Department of Chemical Sciences, University of Johannesburg, Doornfontein, Johannesburg 2050, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7329-7015","authenticated-orcid":false,"given":"Zikhona","family":"Njengele-Tetyana","sequence":"additional","affiliation":[{"name":"Advanced Materials Division, DSI\/Mintek NIC, Mintek, 200 Malibongwe Drive, Randburg 2194, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikiwe","family":"Mhlanga","sequence":"additional","affiliation":[{"name":"Advanced Materials Division, DSI\/Mintek NIC, Mintek, 200 Malibongwe Drive, Randburg 2194, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4436-0932","authenticated-orcid":false,"given":"Phumlani","family":"Tetyana","sequence":"additional","affiliation":[{"name":"Advanced Materials Division, DSI\/Mintek NIC, Mintek, 200 Malibongwe Drive, Randburg 2194, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8121-5617","authenticated-orcid":false,"given":"Amanda","family":"Skepu","sequence":"additional","affiliation":[{"name":"Next Generation Health, Council for Scientific and Industrial Research, Meiring Naude Road, Brummeria, Pretoria 0001, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane Catherine","family":"Ngila","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Johannesburg, Doornfontein, Johannesburg 2050, South Africa"},{"name":"The African Academy of Science, 8 Miotoni Lane, Karen, Nairobi P.O. Box 24916-00502, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0972-3785","authenticated-orcid":false,"given":"Lucky Mashudu","family":"Sikhwivhilu","sequence":"additional","affiliation":[{"name":"Advanced Materials Division, DSI\/Mintek NIC, Mintek, 200 Malibongwe Drive, Randburg 2194, South Africa"},{"name":"Department of Chemistry, Faculty of Science, Engineering and Agriculture, Thohoyandou 0950, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1159\/000149174","article-title":"Bunyaviridae","volume":"14","author":"Bishop","year":"1980","journal-title":"Intervirology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.cll.2017.01.004","article-title":"Rift Valley Fever","volume":"37","author":"Hartman","year":"2017","journal-title":"Clin. 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