{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:56:12Z","timestamp":1788263772131,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19KK0173"],"award-info":[{"award-number":["19KK0173"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Schistosoma mekongi, a blood fluke that causes Asian zoonotic schistosomiasis, is distributed in communities along the Mekong River in Cambodia and Lao People\u2019s Democratic Republic. Decades of employing numerous control measures including mass drug administration using praziquantel have resulted in a decline in the prevalence of schistosomiasis mekongi. This, however, led to a decrease in sensitivity of Kato\u2013Katz stool microscopy considered as the gold standard in diagnosis. In order to develop a serological assay with high sensitivity and specificity which can replace Kato\u2013Katz, recombinant S. mekongi thioredoxin peroxidase-1 protein (rSmekTPx-1) was expressed and produced. Diagnostic performance of the rSmekTPx-1 antigen through ELISA for detecting human schistosomiasis was compared with that of recombinant protein of S. japonicum TPx-1 (rSjTPx-1) using serum samples collected from endemic foci in Cambodia. The sensitivity and specificity of rSmekTPx-1 in ELISA were 89.3% and 93.3%, respectively, while those of rSjTPx-1 were 71.4% and 66.7%, respectively. In addition, a higher Kappa value of 0.82 calculated between rSmekTPx-1 antigen ELISA and Kato\u2013Katz confirmed better agreement than between rSjTPx-1 antigen ELISA and Kato\u2013Katz (Kappa value 0.38). These results suggest that ELISA with rSmekTPx-1 antigen can be a potential diagnostic method for detecting active human S. mekongi infection.<\/jats:p>","DOI":"10.3390\/diagnostics12123077","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T04:00:37Z","timestamp":1670385637000},"page":"3077","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cloning, Expression and Evaluation of Thioredoxin Peroxidase-1 Antigen for the Serological Diagnosis of Schistosoma mekongi Human Infection"],"prefix":"10.3390","volume":"12","author":[{"given":"Atcharaphan","family":"Wanlop","sequence":"first","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2998-9057","authenticated-orcid":false,"given":"Jose Ma. M.","family":"Angeles","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Public Health, University of the Philippines Manila, Manila 1000, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian Miki C.","family":"Macalanda","sequence":"additional","affiliation":[{"name":"Department of Immunopathology and Microbiology, College of Veterinary Medicine and Biomedical Sciences, Cavite State University, Indang 4122, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Kirinoki","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi 321-0293, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuma","family":"Ohari","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Yajima","sequence":"additional","affiliation":[{"name":"Would Health Organization Regional Office for Southeast Asia, New Delhi 110011, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Yamagishi","sequence":"additional","affiliation":[{"name":"International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin Austin L.","family":"Ona","sequence":"additional","affiliation":[{"name":"College of Medicine, University of the Philippines Manila, Manila 1000, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-ichiro","family":"Kawazu","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gordon, C., Kurscheid, J., Williams, G., Clements, A., Li, Y., Zhou, X.-N., Utzinger, J., McManus, D., and Gray, D. 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