{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:12:28Z","timestamp":1773835948913,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health, Czech Republic\u2014conceptual development of research organization","award":["00669806"],"award-info":[{"award-number":["00669806"]}]},{"name":"Ministry of Health, Czech Republic\u2014conceptual development of research organization","award":["CZ.02.1.01\/0.0\/0.0\/16_013\/000167"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_013\/000167"]}]},{"name":"Ministry of Health, Czech Republic\u2014conceptual development of research organization","award":["LM2015089"],"award-info":[{"award-number":["LM2015089"]}]},{"name":"BBMRI-CZ: Biobank network\u2014a versatile platform for the research of the etiopathogenesis of diseases","award":["00669806"],"award-info":[{"award-number":["00669806"]}]},{"name":"BBMRI-CZ: Biobank network\u2014a versatile platform for the research of the etiopathogenesis of diseases","award":["CZ.02.1.01\/0.0\/0.0\/16_013\/000167"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_013\/000167"]}]},{"name":"BBMRI-CZ: Biobank network\u2014a versatile platform for the research of the etiopathogenesis of diseases","award":["LM2015089"],"award-info":[{"award-number":["LM2015089"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>The anti-M\u00fcllerian hormone (AMH) is a glycoprotein that plays an important role in prenatal sex differentiation. It is used as a biomarker in polycystic ovary syndrome (PCOS) diagnostics, as well as for estimating an individual\u2019s ovarian reserve and the ovarian response to hormonal stimulation during in vitro fertilization (IVF). The aim of this study was to test the stability of AMH during various preanalytical conditions that are in accordance with the ISBER (International Society for Biological and Environmental Repositories) protocol. Plasma and serum samples were taken from each of the 26 participants. The samples were then processed according to the ISBER protocol. AMH levels were measured in all the samples simultaneously using the chemiluminescent kit ACCESS AMH in a UniCel\u00ae DxI 800 Immunoassay System (Beckman Coulter, Brea, CA, USA). The study proved that AMH retains a relatively high degree of stability during repeated freezing and thawing in serum. AMH was shown to be less stable in plasma samples. Room temperature proved to be the least suitable condition for the storage of samples before performing the biomarker analysis. During the testing of storage stability at 5\u20137 \u00b0C, the values decreased over time for all the plasma samples but remained stable in the serum samples. We proved that AMH is highly stable under various stress conditions. The anti-M\u00fcllerian hormone retained the greatest stability in the serum samples.<\/jats:p>","DOI":"10.3390\/diagnostics13081501","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T10:11:25Z","timestamp":1682071885000},"page":"1501","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Stability of the Anti-M\u00fcllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions"],"prefix":"10.3390","volume":"13","author":[{"given":"Radana","family":"Vrz\u00e1kov\u00e1","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e1clav","family":"\u0160im\u00e1nek","sequence":"additional","affiliation":[{"name":"Department of Immunochemistry, University Hospital and Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ond\u0159ej","family":"Topol\u010dan","sequence":"additional","affiliation":[{"name":"Department of Immunochemistry, University Hospital and Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladim\u00edr","family":"Vurm","sequence":"additional","affiliation":[{"name":"Department of Immunochemistry, University Hospital and Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3988-7898","authenticated-orcid":false,"given":"David","family":"Slouka","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, University Hospital and Faculty of Medicine in Pilsen, Charles University, 30955 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2739-2302","authenticated-orcid":false,"given":"Radek","family":"Ku\u010dera","sequence":"additional","affiliation":[{"name":"Department of Immunochemistry, University Hospital and Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic"},{"name":"Department of Pharmacology and Toxicology, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00018-020-03576-x","article-title":"New insights into anti-M\u00fcllerian hormone role in the hypothalamic\u2013pituitary\u2013gonadal axis and neuroendocrine development","volume":"78","author":"Silva","year":"2021","journal-title":"Cell. 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