{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:15:39Z","timestamp":1790784939018,"version":"4.1.0"},"reference-count":11,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: M2-type anti-mitochondrial autoantibodies are considered the hallmark of primary biliary cholangitis and are directed mainly against the E2 subunits of the 2-oxo acid dehydrogenase complex enzymes (PDC, BCOADC and OGDC). The aim of this study was to determine whether a Dot-blot that includes these E2 subunits separately could confirm the results of methods with non-separated subunits in patients with low positive or discordant results between techniques. Methods: Sera of 24 patients with low positive or discordant results and of 10 patients with clear positive results by non-separated subunits methods were analyzed by Dot-blot with separated subunits. Results: Autoantibodies against E2 subunits of PDC, BCOADC or OGDC were detected in all patients, except in one case from the low positive or discordant results group, by Dot-blot with separated subunits. Conclusions: It would be advisable to use methods that include the three E2 subunits, and a Dot-blot with separated subunits could confirm doubtful cases by non-separated assays.<\/jats:p>","DOI":"10.3390\/diagnostics13111840","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T01:36:28Z","timestamp":1684978588000},"page":"1840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Detection of M2-Type Anti-Mitochondrial Autoantibodies against Specific Subunits in the Diagnosis of Primary Biliary Cholangitis in Patients with Discordant Results"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7188-5411","authenticated-orcid":false,"given":"Cristina","family":"Arnaldos-P\u00e9rez","sequence":"first","affiliation":[{"name":"Department of Immunology, Centre de Diagn\u00f2stic Biom\u00e8dic, Hospital Cl\u00ednic de Barcelona, Villarroel 170-Escala 4, Planta 0, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1509-8691","authenticated-orcid":false,"given":"Albert","family":"P\u00e9rez-Isidro","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre de Diagn\u00f2stic Biom\u00e8dic, Hospital Cl\u00ednic de Barcelona, Villarroel 170-Escala 4, Planta 0, 08036 Barcelona, Spain"},{"name":"Institut de Recerca Biom\u00e8dica August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uma","family":"Bolos","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre de Diagn\u00f2stic Biom\u00e8dic, Hospital Cl\u00ednic de Barcelona, Villarroel 170-Escala 4, Planta 0, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Dom\u00e8nech","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre de Diagn\u00f2stic Biom\u00e8dic, Hospital Cl\u00ednic de Barcelona, Villarroel 170-Escala 4, Planta 0, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"Ball\u00fas","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre de Diagn\u00f2stic Biom\u00e8dic, Hospital Cl\u00ednic de Barcelona, Villarroel 170-Escala 4, Planta 0, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3189-7557","authenticated-orcid":false,"given":"Sergio","family":"Rodr\u00edguez-Tajes","sequence":"additional","affiliation":[{"name":"Institut de Recerca Biom\u00e8dica August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain"},{"name":"Liver Unit, Hospital Cl\u00ednic de Barcelona, 08036 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-1586","authenticated-orcid":false,"given":"Mar\u00eda Carlota","family":"Londo\u00f1o","sequence":"additional","affiliation":[{"name":"Institut de Recerca Biom\u00e8dica August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain"},{"name":"Liver Unit, Hospital Cl\u00ednic de Barcelona, 08036 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odette","family":"Vi\u00f1as","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre de Diagn\u00f2stic Biom\u00e8dic, Hospital Cl\u00ednic de Barcelona, Villarroel 170-Escala 4, Planta 0, 08036 Barcelona, Spain"},{"name":"Institut de Recerca Biom\u00e8dica August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Est\u00edbaliz","family":"Ruiz-Ortiz","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre de Diagn\u00f2stic Biom\u00e8dic, Hospital Cl\u00ednic de Barcelona, Villarroel 170-Escala 4, Planta 0, 08036 Barcelona, Spain"},{"name":"Institut de Recerca Biom\u00e8dica August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1007\/s12016-021-08904-y","article-title":"Antimitochondrial Antibodies: From Bench to Bedside","volume":"63","author":"Colapietro","year":"2022","journal-title":"Clin. 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