{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T04:04:48Z","timestamp":1784952288746,"version":"3.55.0"},"reference-count":59,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medcial University of Silesia","award":["PCN-1-077\/K\/2\/I"],"award-info":[{"award-number":["PCN-1-077\/K\/2\/I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Some NK cell subpopulations may be involved in the modulation of fibrogenesis in the liver. The aim of the study was to evaluate the relationship between the number and phenotype of NK cell subsets in peripheral blood (PB) and total NK cell percentage, population density and the degree of liver fibrosis of patients infected with hepatitis C virus (HCV+). The study group consisted of 56 HCV+ patients, divided into two subgroups: patients with mild or moderate fibrosis and patients with advanced liver fibrosis or cirrhosis (F \u2265 3 in METAVIR classification). The preparations were stained with H-E and AZAN staining. NK cells were targeted with anti-CD56 antibody and identified automatically in situ using the DakoVision system. Assessment of different NK cell subsets in PB was performed with the flow cytometry technique. In the PB of HCV+ patients with advanced liver fibrosis, there was a lower proportion of CD62L+; CD62L+\/CD94++; CD27+; CD127+\/CD27+ and CXCR3+\/CD27+ NK subsets, as compared to patients with mild\/moderate liver fibrosis. The results also showed no association between total PB NK cell level and total intrahepatic NK cell population density between patients with mild\/moderate fibrosis and with advanced liver fibrosis. However, positive correlations between the PB levels of CD94+ and CD62L+ NK cell subsets and the intrahepatic total NK cell percentage and population density in the liver, irrespectively to the extent of fibrosis, were observed. Additionally, positive correlation was found between the PB CXCR3+\/CD94+ NK cell percentages and intrahepatic NK cell percentages in patients with advanced hepatic fibrosis. Lower blood availability of specific NK subsets in patients with chronic type C hepatitis might be a cause of progression of liver fibrosis via insufficient control over hepatic stellate cells.<\/jats:p>","DOI":"10.3390\/diagnostics13132187","type":"journal-article","created":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:33:07Z","timestamp":1687912387000},"page":"2187","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Association of NK Cells with the Severity of Fibrosis in Patients with Chronic Hepatitis C"],"prefix":"10.3390","volume":"13","author":[{"given":"Anna","family":"Kleczka","sequence":"first","affiliation":[{"name":"Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Ostrog\u00f3rska 30, 41-200 Sosnowiec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan","family":"Mazur","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, 40-808 Zabrze, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Tomaszek","sequence":"additional","affiliation":[{"name":"Department of Pathomorphology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, 40-800 Zabrze, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Gabriel","sequence":"additional","affiliation":[{"name":"Department of Pathomorphology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, 40-800 Zabrze, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6289-7234","authenticated-orcid":false,"given":"Rados\u0142aw","family":"Dzik","sequence":"additional","affiliation":[{"name":"Faculty of Biomedical Engineering, Department of Biosensors and Processing of Biomedical Signals, Silesian University of Technology, Roosevelta 40, 41-800 Zabrze, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3575-8159","authenticated-orcid":false,"given":"Agata","family":"Kaba\u0142a-Dzik","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Ostrog\u00f3rska 30, 41-200 Sosnowiec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.3961\/jpmph.21.151","article-title":"Trends of the Global Hepatitis C Disease Burden: Strategies to Achieve Elimination","volume":"54","author":"Brunner","year":"2021","journal-title":"J. Prev. Med. Public Health"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.jhep.2018.11.013","article-title":"Epidemiology and natural history of hepatitis C virus infection among children and young people","volume":"70","author":"Modin","year":"2018","journal-title":"J. Hepatol."},{"key":"ref_3","unstructured":"Raciborski, F., Gujski, M., K\u0142ak, A., and Gierczy\u0144ski, J. (2015). HCV w Polsce, Raport Instytutu Ochrony Zdrowia."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s41232-016-0005-6","article-title":"Roles of hepatic stellate cells in liver inflammation: A new perspective","volume":"36","author":"Fujita","year":"2016","journal-title":"Inflamm. Regen."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/cld.1014","article-title":"Histological Grading and Staging of Chronic Hepatitis","volume":"17","author":"Krishna","year":"2021","journal-title":"Clin. Liver Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.jhep.2007.07.006","article-title":"Grading and staging systems for inflammation and fibrosis in chronic liver diseases","volume":"47","author":"Goodman","year":"2007","journal-title":"J. Hepatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1002\/hep.510240201","article-title":"An algorithm for the grading of activity in chronic hepatitis C. The METAVIR Cooperative Study Group","volume":"24","author":"Bedossa","year":"1996","journal-title":"Hepatology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/0168-8278(91)90084-O","article-title":"Classification of chronic viral hepatitis: A need for reassessment","volume":"13","author":"Scheuer","year":"1991","journal-title":"J. Hepatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.3748\/wjg.v23.i8.1458","article-title":"Clinical features and natural history of cryptogenic cirrhosis compared to hepatitis C virus-related cirrhosis","volume":"23","author":"Rinaldi","year":"2017","journal-title":"World J. Gastroenterol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Khatun, M., and Ray, R.B. (2019). Mechanisms Underlying Hepatitis C Virus-Associated Hepatic Fibrosis. Cells, 8.","DOI":"10.3390\/cells8101249"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1872-034X.2007.00258.x","article-title":"Hepatocyte steatosis in HCV patients promotes fibrosis by enhancing TGF-beta liver expression","volume":"38","author":"Gabriel","year":"2008","journal-title":"Hepatol Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"712105","DOI":"10.3389\/fcimb.2021.712105","article-title":"Chronic Hepatitis C Pathogenesis: Immune Response in the Liver Microenvironment and Peripheral Compartment","volume":"11","author":"Rios","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.3389\/fimmu.2019.01414","article-title":"What Defines NK Cell Functional Fate: Phenotype or Metabolism?","volume":"10","author":"Poznanski","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.1172\/JCI83476","article-title":"CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo","volume":"126","author":"Tian","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1182\/blood-2009-04-215491","article-title":"CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets","volume":"115","author":"Yu","year":"2010","journal-title":"Blood"},{"key":"ref_16","first-page":"212","article-title":"Human NK cells: From development to effector functions","volume":"27","author":"Arachchige","year":"2021","journal-title":"J. Endotoxin Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e146686","DOI":"10.1172\/JCI146686","article-title":"Natural killer cell immunosuppressive function requires CXCR3-dependent redistribution within lymphoid tissues","volume":"131","author":"Ali","year":"2021","journal-title":"J. Clin. Investig."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Lapa, D., Garbuglia, A.R., Capobianchi, M.R., and Del Porto, P. (2019). Hepatitis C Virus Genetic Variability, Human Immune Response, and Genome Polymorphisms: Which Is the Interplay?. Cells, 8.","DOI":"10.3390\/cells8040305"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/cmi.2016.3","article-title":"Liver immunology and its role in inflammation and homeostasis","volume":"13","author":"Robinson","year":"2016","journal-title":"Cell. Mol. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1002\/hep.29009","article-title":"New classification of liver biopsy assessment for fibrosis in chronic hepatitis B patients before and after treatment","volume":"65","author":"Sun","year":"2016","journal-title":"Hepatology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1084\/jem.20011145","article-title":"Binding of the Hepatitis C Virus Envelope Protein E2 to CD81 Inhibits Natural Killer Cell Functions","volume":"195","author":"Tseng","year":"2001","journal-title":"J. Exp. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"535","DOI":"10.3390\/v6020535","article-title":"CD81 and Hepatitis C Virus (HCV) Infection","volume":"6","author":"Levy","year":"2014","journal-title":"Viruses"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"842831","DOI":"10.3389\/fimmu.2022.842831","article-title":"Negative Regulation and Protective Function of Natural Killer Cells in HIV Infection: Two Sides of a Coin","volume":"13","author":"Sun","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"887186","DOI":"10.3389\/fimmu.2022.887186","article-title":"The Dynamic Role of NK Cells in Liver Cancers: Role in HCC and HBV Associated HCC and Its Therapeutic Implications","volume":"13","author":"Sajid","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Njiomegnie, G.F., Read, S.A., Fewings, N., George, J., McKay, F., and Ahlenstiel, G. (2020). Immunomodulation of the Natural Killer Cell Phenotype and Response during HCV Infection. J. Clin. Med., 9.","DOI":"10.3390\/jcm9041030"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5919","DOI":"10.3748\/wjg.v26.i39.5919","article-title":"Clinical assessment and management of liver fibrosis in non-alcoholic fatty liver disease","volume":"26","year":"2020","journal-title":"World J. Gastroenterol."},{"key":"ref_27","unstructured":"(2016). New Recommendations in the Updated WHO Guidelines for the Screening, Care and Treatment of Persons with Chronic Hepatitis C Infection, World Health Organization. Policy Brief."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1007\/s00428-002-0749-z","article-title":"Feasibility of histological grading and staging of chronic viral hepatitis using specimens obtained by thin-needle biopsy","volume":"442","author":"Petz","year":"2003","journal-title":"Virchows Arch."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"944","DOI":"10.5858\/arpa.2016-0074-OA","article-title":"Implementation of Whole Slide Imaging for Clinical Purposes: Issues to Consider from the Perspective of Early Adopters","volume":"141","author":"Evans","year":"2017","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Jiang, K., Mohammad, M.K., Dar, W.A., Kong, J., and Farris, A.B. (2020). Quantitative assessment of liver fibrosis by digital image analysis reveals correlation with qualitative clinical fibrosis staging in liver transplant patients. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0239624"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.jhep.2009.05.030","article-title":"Fine characterization of intrahepatic NK cells expressing natural killer receptors in chronic hepatitis B and C","volume":"51","author":"Bonorino","year":"2009","journal-title":"J. Hepatol."},{"key":"ref_32","first-page":"301","article-title":"Immunoregulatory Role of NK Cells in Tissue Inflammation and Regeneration","volume":"8","author":"Surette","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"208","DOI":"10.3892\/mmr.2022.12724","article-title":"Crosstalk between NK cells and hepatic stellate cells in liver fibrosis (Review)","volume":"25","author":"Zhang","year":"2022","journal-title":"Mol. Med. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Cosgrove, C., Berger, C.T., Kroy, D.C., Cheney, P.C., Ghebremichael, M., Aneja, J., Tomlinson, M., Kim, A.Y., Lauer, G., and Alter, G. (2014). Chronic HCV Infection Affects the NK Cell Phenotype in the Blood More than in the Liver. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0105950"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3308","DOI":"10.4049\/jimmunol.1300164","article-title":"Hepatitis C Virus\u2013Infected Cells Downregulate NKp30 and Inhibit Ex Vivo NK Cell Functions","volume":"191","author":"Holder","year":"2013","journal-title":"J. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1002\/hep.22413","article-title":"Activation of hepatic stellate cells after phagocytosis of lymphocytes: A novel pathway of fibrogenesis","volume":"48","author":"Muhanna","year":"2008","journal-title":"Hepatology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.jhep.2014.08.038","article-title":"Regulatory CD4+ T cells modulate the interaction between NK cells and hepatic stellate cells by acting on either cell type","volume":"62","author":"Langhans","year":"2015","journal-title":"J. Hepatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/cmi.2016.62","article-title":"Interleukin-33 in the pathogenesis of liver fibrosis: Alarming ILC2 and hepatic stellate cells","volume":"14","author":"Weiskirchen","year":"2016","journal-title":"Cell. Mol. Immunol."},{"key":"ref_39","first-page":"G313","article-title":"Ablation of interaction between IL-33 and ST2+ regulatory T cells increases immune cell-mediated hepatitis and activated NK cell liver infiltration","volume":"311","author":"Noel","year":"2016","journal-title":"Am. J. Physiol. Liver Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.jhep.2009.12.013","article-title":"Abundant numbers of regulatory T cells localize to the liver of chronic hepatitis C infected patients and limit the extent of fibrosis","volume":"52","author":"Claassen","year":"2010","journal-title":"J. Hepatol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/s41420-023-01347-8","article-title":"Regulatory T cells (Tregs) in liver fibrosis","volume":"9","author":"Wu","year":"2023","journal-title":"Cell Death Discov."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1111\/liv.13079","article-title":"IL15 polymorphism is associated with advanced fibrosis, inflammation-related biomarkers and virological response in human immunodeficiency virus\/hepatitis C virus coinfection","volume":"36","author":"Berenguer","year":"2016","journal-title":"Liver Int."},{"key":"ref_43","first-page":"278","article-title":"Zwi\u0105zek kom\u00f3rek NK i subpopulacji limfocyt\u00f3w z post\u0119pem w\u0142\u00f3knienia w przebiegu przewlek\u0142ego zapalenia w\u0105troby typu C","volume":"16","author":"Gabriel","year":"2009","journal-title":"Gastroenterol. Pol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.4049\/jimmunol.1202395","article-title":"CD62L Is Critical for Maturation and Accumulation of Murine Hepatic NK Cells in Response to Viral Infection","volume":"190","author":"Peng","year":"2013","journal-title":"J. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1061959","DOI":"10.3389\/fimmu.2022.1061959","article-title":"Inflammatory-driven NK cell maturation and its impact on pathology","volume":"13","author":"Bourayou","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.3389\/fimmu.2019.02078","article-title":"Human NK Cell Development: One Road or Many?","volume":"10","author":"Cichocki","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1053\/j.gastro.2012.10.039","article-title":"Blocking the Natural Killer Cell Inhibitory Receptor NKG2A Increases Activity of Human Natural Killer Cells and Clears Hepatitis B Virus Infection in Mice","volume":"144","author":"Li","year":"2013","journal-title":"Gastroenterology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3739","DOI":"10.4049\/jimmunol.180.6.3739","article-title":"CD27 Defines Phenotypically and Functionally Different Human NK Cell Subsets","volume":"180","author":"Vossen","year":"2008","journal-title":"J. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1093\/intimm\/dxn022","article-title":"Application of CD27 as a marker for distinguishing human NK cell subsets","volume":"20","author":"Silva","year":"2008","journal-title":"Int. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/s41392-022-01058-z","article-title":"Natural killer cell homing and trafficking in tissues and tumors: From biology to application","volume":"7","author":"Ran","year":"2022","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1038\/icb.2010.171","article-title":"CD27-deficient mice show normal NK-cell differentiation but impaired function upon stimulation","volume":"89","author":"Taveirne","year":"2011","journal-title":"Immunol. Cell Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s12985-016-0507-5","article-title":"Increased CD56bright NK cells in HIV-HCV co-infection and HCV mono-infection are associated with distinctive alterations of their phenotype","volume":"13","author":"Bhardwaj","year":"2016","journal-title":"Virol. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"839684","DOI":"10.1155\/2015\/839684","article-title":"Chemokine Receptor Expression on Normal Blood CD56+NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population","volume":"2015","author":"Lima","year":"2015","journal-title":"J. Immunol. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"21194","DOI":"10.1038\/s41598-021-00455-8","article-title":"Association between immunologic markers and cirrhosis in individuals with chronic hepatitis B","volume":"11","author":"Argirion","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1038\/s41575-021-00444-2","article-title":"Regulation and functional roles of chemokines in liver diseases","volume":"18","author":"Cao","year":"2021","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Eisenhardt, M., Gl\u00e4ssner, A., Kr\u00e4mer, B., K\u00f6rner, C., Sibbing, B., Kokordelis, P., Nischalke, H.D., Sauerbruch, T., Spengler, U., and Nattermann, J. (2012). The CXCR3(+)CD56Bright Phenotype Characterizes a Distinct NK Cell Subset with Anti-Fibrotic Potential That Shows Dys-Regulated Activity in Hepatitis C. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0038846"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e3678","DOI":"10.1097\/MD.0000000000003678","article-title":"Accumulation of Intrahepatic TNF-\u03b1-Producing NKp44+ NK Cells Correlates with Liver Fibrosis and Viral Load in Chronic HCV Infection","volume":"95","author":"Nel","year":"2016","journal-title":"Medicine"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1113\/JP281061","article-title":"Hepatic stellate cells in physiology and pathology","volume":"600","author":"Kamm","year":"2022","journal-title":"J. Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Fugier, E., Marche, H., Thelu, M.A., Macek Jilkova, Z., Van Campenhout, N., Dufeu-Duchesne, T., Leroy, V., Zarski, J.P., Sturm, N., and Marche, P.N. (2014). Functions of Liver Natural Killer Cells Are Dependent on the Severity of Liver Inflammation and Fibrosis in Chronic Hepatitis C. 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