{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T21:36:39Z","timestamp":1785188199471,"version":"3.55.0"},"reference-count":112,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T00:00:00Z","timestamp":1690675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health and Welfare, Republic of Korea","award":["HI21C1552"],"award-info":[{"award-number":["HI21C1552"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>As the prevalence of pregnancies with advanced maternal age increases, the risk of fetal chromosomal abnormalities is on the rise. Therefore, prenatal genetic screening and diagnosis have become essential elements in contemporary obstetrical care. Trophoblast retrieval and isolation from the cervix (TRIC) is a non-invasive procedure that can be utilized for prenatal genetic diagnosis. The method involves the isolation of fetal cells (extravillous trophoblasts) by transcervical sampling; along with its non-invasiveness, TRIC exhibits many other advantages such as its usefulness in early pregnancy at 5 weeks of gestation, and no interference by various fetal and maternal factors. Moreover, the trophoblast yields from TRIC can provide valuable information about obstetrical complications related to abnormal placentation even before clinical symptoms arise. The standardization of this clinical tool is still under investigation, and the upcoming advancements in TRIC are expected to meet the increasing need for a safe and accurate option for prenatal diagnosis.<\/jats:p>","DOI":"10.3390\/diagnostics13152532","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T01:39:32Z","timestamp":1690767572000},"page":"2532","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Novel Paradigm for Non-Invasive Prenatal Genetic Screening: Trophoblast Retrieval and Isolation from the Cervix (TRIC)"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8258-9696","authenticated-orcid":false,"given":"Kirim","family":"Hong","sequence":"first","affiliation":[{"name":"CHA Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 06125, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0538-3517","authenticated-orcid":false,"given":"Hee Jin","family":"Park","sequence":"additional","affiliation":[{"name":"CHA Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 06125, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4127-4748","authenticated-orcid":false,"given":"Hee Yeon","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Han","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon","family":"Jang","sequence":"additional","affiliation":[{"name":"CHA Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 06125, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1118-4920","authenticated-orcid":false,"given":"Soo Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"CHA Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 06125, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0722-1714","authenticated-orcid":false,"given":"Dong Hyun","family":"Cha","sequence":"additional","affiliation":[{"name":"CHA Gangnam Medical Center, Department of Obstetrics and Gynecology, CHA University, Seoul 06125, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,30]]},"reference":[{"key":"ref_1","first-page":"17","article-title":"Births: Final Data for 2020","volume":"70","author":"Osterman","year":"2021","journal-title":"Natl. Vital. Stat. Rep."},{"key":"ref_2","unstructured":"OECD (2022). OECD Economic Surveys: Korea 2022, OECD Publishing."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.bpobgyn.2020.07.005","article-title":"Advanced maternal age and adverse pregnancy outcomes","volume":"70","author":"Frick","year":"2021","journal-title":"Best Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"751","DOI":"10.2147\/IJWH.S283216","article-title":"Management of Pregnancy in Women of Advanced Maternal Age: Improving Outcomes for Mother and Baby","volume":"13","author":"Glick","year":"2021","journal-title":"Int. J. Womens Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"B25","DOI":"10.1016\/j.ajog.2022.07.022","article-title":"Obstetric Care Consensus #11, Pregnancy at age 35 years or older","volume":"228","author":"Gantt","year":"2022","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1002\/uog.14636","article-title":"Procedure-related risk of miscarriage following amniocentesis and chorionic villus sampling: A systematic review and meta-analysis","volume":"45","author":"Akolekar","year":"2015","journal-title":"Ultrasound Obs. Gynecol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1146\/annurev-genom-083118-015053","article-title":"The Emergence and Global Spread of Noninvasive Prenatal Testing","volume":"22","author":"Ravitsky","year":"2021","journal-title":"Annu. Rev. Genom. Hum. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"113","DOI":"10.2147\/IJWH.S67124","article-title":"Non-invasive prenatal testing: A review of international implementation and challenges","volume":"7","author":"Allyse","year":"2015","journal-title":"Int. J. Womens Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1007\/s00404-020-05846-2","article-title":"Prenatal tests for chromosomal abnormalities detection (PTCAD): Pregnant women\u2019s knowledge in an Italian Population","volume":"303","author":"Quaresima","year":"2021","journal-title":"Arch Gynecol Obstet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e057658","DOI":"10.1136\/bmjopen-2021-057658","article-title":"Non-invasive prenatal testing in mitigating concerns from invasive prenatal diagnostic testing: Retrospective assessment of utility in an academic healthcare system in the US","volume":"12","author":"Kim","year":"2022","journal-title":"BMJ Open"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"E5503","DOI":"10.1073\/pnas.1508736112","article-title":"Plasma DNA tissue mapping by genome-wide methylation sequencing for noninvasive prenatal, cancer, and transplantation assessments","volume":"112","author":"Sun","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"S64","DOI":"10.1016\/j.placenta.2013.11.014","article-title":"Review: Cell-free fetal DNA in the maternal circulation as an indication of placental health and disease","volume":"35","author":"Taglauer","year":"2014","journal-title":"Placenta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1002\/pd.1700","article-title":"Free fetal DNA in maternal plasma in anembryonic pregnancies: Confirmation that the origin is the trophoblast","volume":"27","author":"Alberry","year":"2007","journal-title":"Prenat. Diagn."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1086\/301800","article-title":"Quantitative analysis of fetal DNA in maternal plasma and serum: Implications for noninvasive prenatal diagnosis","volume":"62","author":"Lo","year":"1998","journal-title":"Am. J. Hum. Genet"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.ejogrb.2020.08.001","article-title":"Non-invasive prenatal diagnosis and screening for monogenic disorders","volume":"253","author":"Scotchman","year":"2020","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"61ra91","DOI":"10.1126\/scitranslmed.3001720","article-title":"Maternal plasma DNA sequencing reveals the genome-wide genetic and mutational profile of the fetus","volume":"2","author":"Lo","year":"2010","journal-title":"Sci. Transl. Med."},{"key":"ref_17","first-page":"E5106","article-title":"Size-tagged preferred ends in maternal plasma DNA shed light on the production mechanism and show utility in noninvasive prenatal testing","volume":"115","author":"Sun","year":"2018","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e2114937118","DOI":"10.1073\/pnas.2114937118","article-title":"Single-molecule sequencing reveals a large population of long cell-free DNA molecules in maternal plasma","volume":"118","author":"Yu","year":"2021","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"c7401","DOI":"10.1136\/bmj.c7401","article-title":"Non-invasive prenatal assessment of trisomy 21 by multiplexed maternal plasma DNA sequencing: Large scale validity study","volume":"342","author":"Chiu","year":"2011","journal-title":"BMJ"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/uog.12513","article-title":"Non-invasive prenatal testing for aneuploidy: Current status and future prospects","volume":"42","author":"Benn","year":"2013","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"20458","DOI":"10.1073\/pnas.0810641105","article-title":"Noninvasive prenatal diagnosis of fetal chromosomal aneuploidy by massively parallel genomic sequencing of DNA in maternal plasma","volume":"105","author":"Chiu","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"16266","DOI":"10.1073\/pnas.0808319105","article-title":"Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood","volume":"105","author":"Fan","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1002\/pd.4103","article-title":"Validation of targeted sequencing of single-nucleotide polymorphisms for non-invasive prenatal detection of aneuploidy of chromosomes 13, 18, 21, X, and Y","volume":"33","author":"Nicolaides","year":"2013","journal-title":"Prenat. Diagn."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e311","DOI":"10.1016\/j.ajog.2012.01.030","article-title":"Noninvasive prenatal detection and selective analysis of cell-free DNA obtained from maternal blood: Evaluation for trisomy 21 and trisomy 18","volume":"206","author":"Sparks","year":"2012","journal-title":"Am J Obs. Gynecol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1111\/jog.14268","article-title":"Update on noninvasive prenatal testing: A review based on current worldwide research","volume":"46","author":"Samura","year":"2020","journal-title":"J Obs. Gynaecol Res"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1373\/clinchem.2003.024893","article-title":"Size distributions of maternal and fetal DNA in maternal plasma","volume":"50","author":"Chan","year":"2004","journal-title":"Clin. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.ejogrb.2021.01.008","article-title":"Non invasive prenatal testing (NIPT) for common aneuploidies and beyond","volume":"258","author":"Alberry","year":"2021","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1056\/NEJMoa043693","article-title":"First-trimester or second-trimester screening, or both, for Down\u2019s syndrome","volume":"353","author":"Malone","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1097\/AOG.0b013e31824fb482","article-title":"Genome-wide fetal aneuploidy detection by maternal plasma DNA sequencing","volume":"119","author":"Bianchi","year":"2012","journal-title":"Obs. Gynecol"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1002\/pd.3993","article-title":"Noninvasive prenatal aneuploidy testing of chromosomes 13, 18, 21, X, and Y, using targeted sequencing of polymorphic loci","volume":"32","author":"Zimmermann","year":"2012","journal-title":"Prenat. Diagn."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e48","DOI":"10.1097\/AOG.0000000000004084","article-title":"Screening for Fetal Chromosomal Abnormalities: ACOG Practice Bulletin, Number 226","volume":"136","author":"Rose","year":"2020","journal-title":"Obstet. Gynecol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"100336","DOI":"10.1016\/j.gim.2022.11.004","article-title":"Noninvasive prenatal screening (NIPS) for fetal chromosome abnormalities in a general-risk population: An evidence-based clinical guideline of the American College of Medical Genetics and Genomics (ACMG)","volume":"25","author":"Dungan","year":"2023","journal-title":"Genet Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1097\/00003081-199612000-00009","article-title":"Prenatal diagnosis using fetal cells isolated from maternal peripheral blood: A review","volume":"39","author":"Steele","year":"1996","journal-title":"Clin Obs. Gynecol"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1146\/annurev-med-091014-115715","article-title":"Prenatal Diagnosis Innovation: Genome Sequencing of Maternal Plasma","volume":"67","author":"Wong","year":"2016","journal-title":"Annu. Rev. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1002\/pd.4780","article-title":"The importance of determining the limit of detection of non-invasive prenatal testing methods","volume":"36","author":"Fiorentino","year":"2016","journal-title":"Prenat. Diagn."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1002\/pd.3919","article-title":"A multifactorial relationship exists between total circulating cell-free DNA levels and maternal BMI","volume":"32","author":"Vora","year":"2012","journal-title":"Prenat. Diagn."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1111\/aogs.13848","article-title":"Noninvasive prenatal testing and maternal obesity: A review","volume":"99","author":"Juul","year":"2020","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3657","DOI":"10.1080\/14767058.2019.1689558","article-title":"The association of HBB-related significant hemoglobinopathies and low fetal fraction on noninvasive prenatal screening for fetal aneuploidy","volume":"34","author":"Putra","year":"2021","journal-title":"J. Matern. Fetal. Neonatal. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1002\/pd.5436","article-title":"Effects of medication intake in early pregnancy on the fetal fraction of cell-free DNA testing","volume":"39","author":"Chiossi","year":"2019","journal-title":"Prenat. Diagn."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"812781","DOI":"10.3389\/fped.2022.812781","article-title":"Factors Affecting the Fetal Fraction in Noninvasive Prenatal Screening: A Review","volume":"10","author":"Deng","year":"2022","journal-title":"Front. Pediatr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"E5302","DOI":"10.1073\/pnas.1421126111","article-title":"Plasma DNA aberrations in systemic lupus erythematosus revealed by genomic and methylomic sequencing","volume":"111","author":"Chan","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/jhg.2016.25","article-title":"Fetal cell-free DNA fraction in maternal plasma is affected by fetal trisomy","volume":"61","author":"Suzumori","year":"2016","journal-title":"J. Hum. Genet."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1373\/clinchem.2013.202663","article-title":"Integration of noninvasive DNA testing for aneuploidy into prenatal care: What has happened since the rubber met the road?","volume":"60","author":"Bianchi","year":"2014","journal-title":"Clin. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1002\/pd.4625","article-title":"Factors affecting levels of circulating cell-free fetal DNA in maternal plasma and their implications for noninvasive prenatal testing","volume":"35","author":"Kinnings","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1002\/uog.17484","article-title":"Analysis of cell-free DNA in maternal blood in screening for aneuploidies: Updated meta-analysis","volume":"50","author":"Gil","year":"2017","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s40246-019-0244-0","article-title":"Factors affecting cell-free DNA fetal fraction: Statistical analysis of 13,661 maternal plasmas for non-invasive prenatal screening","volume":"13","author":"Hou","year":"2019","journal-title":"Hum Genom."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1002\/pd.5049","article-title":"Discordant non-invasive prenatal testing (NIPT)\u2014A systematic review","volume":"37","author":"Hartwig","year":"2017","journal-title":"Prenat. Diagn."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1002\/pd.4656","article-title":"Interpreting mosaicism in chorionic villi: Results of a monocentric series of 1001 mosaics in chorionic villi with follow-up amniocentesis","volume":"35","author":"Malvestiti","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1136\/jmg.33.7.529","article-title":"Confined placental mosaicism","volume":"33","author":"Kalousek","year":"1996","journal-title":"J. Med. Genet."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/S0095-5108(18)30550-5","article-title":"Chromosomal Mosaicism in Chorionic Villus Sampling","volume":"17","author":"Schreck","year":"1990","journal-title":"Clin. Perinatol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1002\/pd.1970111002","article-title":"Confirmation of CVS mosaicism in term placentae and high frequency of intrauterine growth retardation association with confined placental mosaicism","volume":"11","author":"Kalousek","year":"1991","journal-title":"Prenat. Diagn."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1002\/pd.4659","article-title":"The type of feto-placental aneuploidy detected by cfDNA testing may influence the choice of confirmatory diagnostic procedure","volume":"35","author":"Grati","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1111\/jog.13373","article-title":"Current status of non-invasive prenatal testing in Japan","volume":"43","author":"Samura","year":"2017","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1038\/gim.2014.3","article-title":"Fetoplacental mosaicism: Potential implications for false-positive and false-negative noninvasive prenatal screening results","volume":"16","author":"Grati","year":"2014","journal-title":"Genet. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.tjog.2019.11.003","article-title":"Causes of aberrant non-invasive prenatal testing for aneuploidy: A systematic review","volume":"59","author":"Samura","year":"2020","journal-title":"Taiwan J Obs. Gynecol"},{"key":"ref_56","first-page":"338","article-title":"Placental mosaicism and intrauterine survival of trisomies 13 and 18","volume":"44","author":"Kalousek","year":"1989","journal-title":"Am. J. Hum. Genet"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2426","DOI":"10.1200\/JCO.21.02260","article-title":"Noninvasive Prenatal Test Results Indicative of Maternal Malignancies: A Nationwide Genetic and Clinical Follow-Up Study","volume":"40","author":"Heesterbeek","year":"2022","journal-title":"J. Clin. Oncol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1634\/theoncologist.2002-0279","article-title":"Coexistence of Pregnancy and Malignancy","volume":"7","author":"Pavlidis","year":"2002","journal-title":"Oncol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1002\/pd.4629","article-title":"Uterine leiomyoma confounding a noninvasive prenatal test result","volume":"35","author":"Dharajiya","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/jcu.1870040104","article-title":"Ultrasonic assessment of the high rate of human multiple pregnancy in the first trimester","volume":"4","author":"Levi","year":"1976","journal-title":"J. Clin. Ultrasound"},{"key":"ref_61","first-page":"829","article-title":"Non-invasive prenatal testing (NIPT) in twin pregnancies affected by early single fetal demise: A systematic review of NIPT and vanishing twins","volume":"42","author":"Bekker","year":"2023","journal-title":"Prenat. Diagn."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1016\/j.fertnstert.2009.11.022","article-title":"Transcervical retrieval of fetal cells in the practice of modern medicine: A review of the current literature and future direction","volume":"93","author":"Imudia","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1038\/230052b0","article-title":"Use of the Y chromosome in prenatal sex determination","volume":"230","author":"Shettles","year":"1971","journal-title":"Nature"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/S0140-6736(71)92646-8","article-title":"Unreliability of fetal sexing using cervical material","volume":"2","author":"Bobrow","year":"1971","journal-title":"Lancet"},{"key":"ref_65","first-page":"300","article-title":"Unreliability of prenatal determination of fetal sex with the use of Y-body fluorescence in midcervical smears","volume":"130","author":"Amankwah","year":"1978","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/0140-6736(93)92816-C","article-title":"Detection of trisomy 18 and Y-derived sequences in fetal nucleated cells obtained by transcervical flushing","volume":"342","author":"Adinolfi","year":"1993","journal-title":"Lancet"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/pd.1970150108","article-title":"Molecular evidence of fetal-derived chromosome 21 markers (STRs) in transcervical samples","volume":"15","author":"Adinolfi","year":"1995","journal-title":"Prenat. Diagn."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S0140-6736(95)90306-2","article-title":"Prenatal detection of fetal RhD DNA sequences in transcervical samples","volume":"345","author":"Adinolfi","year":"1995","journal-title":"Lancet"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1093\/humrep\/deq035","article-title":"Endoglandular trophoblast, an alternative route of trophoblast invasion? Analysis with novel confrontation co-culture models","volume":"25","author":"Moser","year":"2010","journal-title":"Hum. Reprod."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.fertnstert.2014.04.008","article-title":"Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation","volume":"102","author":"Bolnick","year":"2014","journal-title":"Fertil. Steril."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1002\/pd.511","article-title":"HLA-G positive trophoblastic cells in transcervical samples and their isolation and analysis by laser microdissection and QF-PCR","volume":"23","author":"Bulmer","year":"2003","journal-title":"Prenat. Diagn."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1159\/000053942","article-title":"The Role of Integrins in Human Embryo Implantation","volume":"16","author":"Merviel","year":"2001","journal-title":"Fetal Diagn. Ther."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.placenta.2010.11.008","article-title":"The art of identification of extravillous trophoblast","volume":"32","author":"Moser","year":"2011","journal-title":"Placenta"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s00418-018-1699-0","article-title":"Human trophoblast invasion: New and unexpected routes and functions","volume":"150","author":"Moser","year":"2018","journal-title":"Histochem. Cell Biol."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Lee, M.J., Kim, S.H., Shim, S.H., Jang, H.Y., Park, H.J., and Cha, D.H. (2020). Optimization Protocol of Fixation Method for Trophoblast Retrieval from the Cervix (TRIC): A Preliminary Study. Diagnostics, 10.","DOI":"10.3390\/diagnostics10050300"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1002\/pd.6260","article-title":"Tricky TRIC: A replication study using trophoblast retrieval and isolation from the cervix to study genetic birth defects","volume":"42","author":"Boussata","year":"2022","journal-title":"Prenat. Diagn."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1002\/pd.4681","article-title":"Trophoblast retrieval and isolation from the cervix (TRIC) is unaffected by early gestational age or maternal obesity","volume":"35","author":"Fritz","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1093\/humupd\/dmy008","article-title":"Trophoblast retrieval and isolation from the cervix: Origins of cervical trophoblasts and their potential value for risk assessment of ongoing pregnancies","volume":"24","author":"Moser","year":"2018","journal-title":"Hum. Reprod. Update"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2086","DOI":"10.1093\/humrep\/dep206","article-title":"Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: A pilot study","volume":"24","author":"Imudia","year":"2009","journal-title":"Hum. Reprod."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"363re364","DOI":"10.1126\/scitranslmed.aah4661","article-title":"Fetal genome profiling at 5 weeks of gestation after noninvasive isolation of trophoblast cells from the endocervical canal","volume":"8","author":"Jain","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.placenta.2015.11.002","article-title":"Cervical trophoblasts for non-invasive single-cell genotyping and prenatal diagnosis","volume":"37","author":"Pfeifer","year":"2016","journal-title":"Placenta"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1515\/jpm-2021-0291","article-title":"Rarity of fetal cells in exocervical samples for noninvasive prenatal diagnosis","volume":"50","author":"Bourlard","year":"2022","journal-title":"J. Perinat. Med."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"S27","DOI":"10.1016\/j.placenta.2017.04.021","article-title":"Quo vadis, trophoblast? Exploring the new ways of an old cell lineage","volume":"60","author":"Drewlo","year":"2017","journal-title":"Placenta"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1903354","DOI":"10.1002\/advs.201903354","article-title":"Nondestructive Identification of Rare Trophoblastic Cells by Endoplasmic Reticulum Staining for Noninvasive Prenatal Testing of Monogenic Diseases","volume":"7","author":"Huang","year":"2020","journal-title":"Adv. Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1002\/pd.1970151008","article-title":"Methods for the transcervical collection of fetal cells during the first trimester of pregnancy","volume":"15","author":"Rodeck","year":"1995","journal-title":"Prenat. Diagn."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1002\/pd.1970151009","article-title":"Detection of fetal cells in transcervical samples and prenatal diagnosis of chromosomal abnormalities","volume":"15","author":"Adinolfi","year":"1995","journal-title":"Prenat. Diagn."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1002\/pd.469","article-title":"Strategies for the isolation and detection of fetal cells in transcervical samples","volume":"22","author":"Bussani","year":"2002","journal-title":"Prenat. Diagn."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1136\/jmg.34.4.302","article-title":"Prenatal detection of fetal aneuploidies using transcervical cell samples","volume":"34","author":"Sherlock","year":"1997","journal-title":"J. Med. Genet."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Hong, K., Jang, H.Y., Shim, S.H., Cho, H.Y., and Cha, D.H. (2023). Advanced Strategy of Trophoblasts Retrieval and Isolation from the Cervix (TRIC): Comparison of Two HLA-G Antibodies for Immunomagnetic Isolation of Trophoblasts. J. Pers. Med., 13.","DOI":"10.3390\/jpm13010022"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"S104","DOI":"10.1053\/plac.2001.0647","article-title":"Cell culture models of human trophoblast II: Trophoblast cell lines\u2014A workshop report","volume":"22","author":"Shiverick","year":"2001","journal-title":"Placenta"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1177\/1933719116632922","article-title":"Trophoblast Retrieval and Isolation From the Cervix for Noninvasive, First Trimester, Fetal Gender Determination in a Carrier of Congenital Adrenal Hyperplasia","volume":"23","author":"Bolnick","year":"2016","journal-title":"Reprod. Sci."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/BF03256231","article-title":"Prenatal diagnosis of common aneuploidies in transcervical samples using quantitative fluorescent-PCR analysis","volume":"11","author":"Bussani","year":"2007","journal-title":"Mol. Diagn. Ther."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1034\/j.1399-0004.1999.560503.x","article-title":"Transcervical cells and the prenatal diagnosis of haemoglobin (Hb) mutations","volume":"56","author":"Cirigliano","year":"1999","journal-title":"Clin. Genet."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1002\/(SICI)1097-0223(199706)17:6<539::AID-PD106>3.0.CO;2-8","article-title":"Prenatal detection of Hb mutations using transcervical cells","volume":"17","author":"Adinolfi","year":"1997","journal-title":"Prenat. Diagn."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1016\/S0198-8859(00)00207-X","article-title":"Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: A comparative study","volume":"61","author":"Blaschitz","year":"2000","journal-title":"Hum. Immunol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/bs.ai.2015.04.001","article-title":"HLA-G: An Immune Checkpoint Molecule","volume":"127","author":"Carosella","year":"2015","journal-title":"Adv. Immunol."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Furukawa, A., Meguro, M., Yamazaki, R., Watanabe, H., Takahashi, A., Kuroki, K., and Maenaka, K. (2019). Evaluation of the Reactivity and Receptor Competition of HLA-G Isoforms toward Available Antibodies: Implications of Structural Characteristics of HLA-G Isoforms. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20235947"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"e13614","DOI":"10.1111\/aji.13614","article-title":"Isolation and characterization of uterine leukocytes collected using a uterine swab technique","volume":"88","author":"Parasar","year":"2022","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/j.1600-0897.2009.00794.x","article-title":"The unique properties of uterine NK cells","volume":"63","author":"Manaster","year":"2010","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1002\/uog.18979","article-title":"Prenatal diagnostic testing and atypical chromosome abnormalities following combined first-trimester screening: Implications for contingent models of non-invasive prenatal testing","volume":"51","author":"Lindquist","year":"2018","journal-title":"Ultrasound Obs. Gynecol"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1097\/AOG.0000000000000452","article-title":"Chromosome abnormalities detected by current prenatal screening and noninvasive prenatal testing","volume":"124","author":"Norton","year":"2014","journal-title":"Obstet. Gynecol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/uog.13270","article-title":"Potential diagnostic consequences of applying non-invasive prenatal testing: Population-based study from a country with existing first-trimester screening","volume":"43","author":"Petersen","year":"2014","journal-title":"Ultrasound Obstet. Gynecol"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1159\/000492152","article-title":"Population-Based Screening for Trisomies and Atypical Chromosomal Abnormalities: Improving Efficacy using the Combined First Trimester Screening Algorithm as well as Individual Risk Parameters","volume":"45","author":"Vogel","year":"2019","journal-title":"Fetal Diagn. Ther."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.ajog.2022.07.034","article-title":"Pregnancy outcome of confined placental mosaicism: Meta-analysis of cohort studies","volume":"227","author":"Spinillo","year":"2022","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_105","doi-asserted-by":"crossref","unstructured":"Hong, K., Kim, S.H., Cha, D.H., and Park, H.J. (2021). Defective Uteroplacental Vascular Remodeling in Preeclampsia: Key Molecular Factors Leading to Long Term Cardiovascular Disease. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222011202"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"252","DOI":"10.3109\/10641955.2011.638955","article-title":"Expression of human leukocyte antigen-G during normal placentation and in preeclamptic pregnancies","volume":"31","author":"Zhu","year":"2012","journal-title":"Hypertens Pregnancy"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/S0002-9378(94)70134-2","article-title":"Expression of the nonclassic histocompatibility antigen HLA-G by preeclamptic placenta","volume":"170","author":"Colbern","year":"1994","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"32382","DOI":"10.1038\/srep32382","article-title":"Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease","volume":"6","author":"Bolnick","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1161\/HYPERTENSIONAHA.117.10182","article-title":"Meta-Analysis and Systematic Review to Assess the Role of Soluble FMS-Like Tyrosine Kinase-1 and Placenta Growth Factor Ratio in Prediction of Preeclampsia","volume":"71","author":"Agrawal","year":"2018","journal-title":"Hypertension"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1161\/HYPERTENSIONAHA.119.12760","article-title":"Short-Term Prediction of Adverse Outcomes Using the sFlt-1 (Soluble fms-Like Tyrosine Kinase 1)\/PlGF (Placental Growth Factor) Ratio in Asian Women With Suspected Preeclampsia","volume":"74","author":"Bian","year":"2019","journal-title":"Hypertension"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"e021376","DOI":"10.1161\/JAHA.120.021376","article-title":"Decision Threshold for Kryptor sFlt-1\/PlGF Ratio in Women With Suspected Preeclampsia: Retrospective Study in a Routine Clinical Setting","volume":"10","author":"Andersen","year":"2021","journal-title":"J. Am. Heart Assoc."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1161\/HYPERTENSIONAHA.122.19070","article-title":"Omeprazole Administration in Preterm Preeclampsia: A Randomized Controlled Trial to Study Its Effect on sFlt-1 (Soluble Fms-Like Tyrosine Kinase-1), PlGF (Placental Growth Factor), and ET-1 (Endothelin-1)","volume":"79","author":"Neuman","year":"2022","journal-title":"Hypertension"}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/13\/15\/2532\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:22:31Z","timestamp":1760127751000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/13\/15\/2532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,30]]},"references-count":112,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["diagnostics13152532"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics13152532","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,30]]}}}