{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:13:58Z","timestamp":1790144038981,"version":"4.0.1"},"reference-count":48,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T00:00:00Z","timestamp":1694563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Systemic sclerosis (SSc) and dermatomyositis (DM) are autoimmune collagen diseases. Specific autoantibodies are known to be involved in their pathogeneses, each presenting with a different clinical manifestation. Although immunoprecipitation is the gold standard method for detecting autoantibodies, it is difficult to perform in all cases owing to the use of radioisotopes. In this study, we developed a new detection method for SSc and DM autoantibodies (A-cube) using cell-free protein synthesis and examined its validity. Proteins were synthesized using wheat germ cell-free protein synthesis. A total of 100 cases of SSc, 50 cases of DM, and 82 healthy controls were examined. The validity of the method was examined by a comparison with existing test results. Anti-centromere antibody, anti-topoisomerase I antibody, anti-RNA polymerase III antibody, anti-U1RNP anti-body, anti-Jo-1 antibody, anti-TIF1\u03b3 antibody, anti-Mi-2 antibody, and anti-ARS antibody were tested for. The results suggested that A-cube is comparable with existing testing methods or has a high sensitivity or specificity. In addition, there was a case in which the diagnosis was reconsidered using the A-cube. The quality of the A-cube was ensured, and its usefulness for a comprehensive analysis was demonstrated. The A-cube can therefore contribute to the clinical assessment and treatment of SSc and DM.<\/jats:p>","DOI":"10.3390\/diagnostics13182929","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T05:08:21Z","timestamp":1694581701000},"page":"2929","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Autoantibody Array Assay: A Novel Autoantibody Detection Method"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7649-0806","authenticated-orcid":false,"given":"Yuta","family":"Norimatsu","sequence":"first","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"},{"name":"Department of Dermatology, International University of Health and Welfare Narita Hospital, Chiba 286-8520, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki Mitsuru","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 100-0013, Japan"},{"name":"ProteoBridge Corporation, Tokyo 135-0064, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Ono","sequence":"additional","affiliation":[{"name":"ProteoBridge Corporation, Tokyo 135-0064, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taishi","family":"Okumura","sequence":"additional","affiliation":[{"name":"ProteoBridge Corporation, Tokyo 135-0064, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emi","family":"Kogo","sequence":"additional","affiliation":[{"name":"ProteoBridge Corporation, Tokyo 135-0064, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirohito","family":"Kotani","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruyoshi","family":"Hisamoto","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai","family":"Kuzumi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6093-1881","authenticated-orcid":false,"given":"Takemichi","family":"Fukasawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"},{"name":"Department of Clinical Cannabinoid Research, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asako","family":"Yoshizaki-Ogawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Goshima","sequence":"additional","affiliation":[{"name":"Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 100-0013, Japan"},{"name":"ProteoBridge Corporation, Tokyo 135-0064, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8194-9140","authenticated-orcid":false,"given":"Ayumi","family":"Yoshizaki","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"},{"name":"Department of Clinical Cannabinoid Research, The University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0889-857X(03)00023-1","article-title":"Natural History of Systemic Sclerosis and the Assessment of Disease Activity, Severity, Functional Status, and Psychologic Well-Being","volume":"29","author":"Medsger","year":"2003","journal-title":"Rheum. 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