{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:05:47Z","timestamp":1792037147671,"version":"4.4.0"},"reference-count":107,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T00:00:00Z","timestamp":1694995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Allergy and Infectious Diseases","award":["U01AI136033"],"award-info":[{"award-number":["U01AI136033"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Leishmaniasis, a neglected tropical disease, encompasses a spectrum of clinical conditions and poses a significant risk of infection to over one billion people worldwide. Visceral leishmaniasis (VL) in the Indian sub-continent (ISC), where the causative parasite is Leishmania donovani, is targeted for elimination by 2025, with some countries already reaching such targets. Other clinical phenotypes due to the same species could act as a reservoir of parasites and thus pose a challenge to successful control and elimination. Sri Lanka has consistently reported cutaneous leishmaniasis (CL) due to L. donovani as the primary disease presentation over several decades. Similar findings of atypical phenotypes of L. donovani have also been reported from several other countries\/regions in the Old World. In this review, we discuss the applicability of different methods in diagnosing CL due to L. donovani and a comprehensive assessment of diagnostic methods spanning clinical, microscopic, molecular, and immunological approaches. By incorporating evidence from Sri Lanka and other regions on L. donovani-related CL, we thoroughly evaluate the accuracy, feasibility, and relevance of these diagnostic tools. We also discuss the challenges and complexities linked to diagnosing CL and review novel approaches and their applicability for detecting CL.<\/jats:p>","DOI":"10.3390\/diagnostics13182989","type":"journal-article","created":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T02:47:52Z","timestamp":1695091672000},"page":"2989","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Diagnostic Tools for Cutaneous Leishmaniasis Caused by Leishmania donovani: A Narrative Review"],"prefix":"10.3390","volume":"13","author":[{"given":"Sachee Bhanu","family":"Piyasiri","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 0800, Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2041-6017","authenticated-orcid":false,"given":"Rajika","family":"Dewasurendra","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 0800, Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilakshi","family":"Samaranayake","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 0800, Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadira","family":"Karunaweera","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 0800, Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,18]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization Factsheets (2023, June 16). Leishmaniasis. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/leishmaniasis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.4269\/ajtmh.17-0162","article-title":"Case report: Old world mucosal leishmaniasis: Report of five imported cases to the hospital for tropical diseases, London, United Kingdom","volume":"97","author":"Patel","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Thakur, L., Singh, K.K., Shanker, V., Negi, A., Jain, A., Matlashewski, G., and Jain, M. (2018). Atypical leishmaniasis: A global perspective with emphasis on the Indian subcontinent. PLoS Neglected Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006659"},{"key":"ref_4","unstructured":"(2023, June 12). World Health Organization Global Health Observatory, Leishmaniasis. Available online: http:\/\/www.who.int\/gho\/neglected_diseases\/leishmaniasis\/en\/."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Alvar, J., V\u00e9lez, I.D., Bern, C., Herrero, M., Desjeux, P., Cano, J., Jannin, J., Boer, M.D., and WHO Leishmaniasis Control Team (2012). Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0035671"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"906","DOI":"10.4269\/ajtmh.2011.11-0055","article-title":"Case report: Disseminated cutaneous leishmaniasis resembling post-kala-azar dermal leishmaniasis caused by Leishmania donovani in three patients co-infected with visceral leishmaniasis and human immunodeficiency virus\/acquired immunodeficiency syndrome in Ethiopia","volume":"84","author":"Gelanew","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e07282","DOI":"10.1016\/j.heliyon.2021.e07282","article-title":"Typical and atypical cutaneous leishmaniasis in Himachal Pradesh (India)","volume":"7","author":"Lata","year":"2021","journal-title":"Heliyon"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/1756-3305-7-123","article-title":"Multilocus microsatellite typing reveals a genetic relationship but, also, genetic differences between Indian strains of Leishmania tropica causing cutaneous leishmaniasis and those causing visceral leishmaniasis","volume":"7","author":"Krayter","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1099\/jmm.0.076695-0","article-title":"Cutaneous leishmaniasis caused by Leishmania donovani in the tribal population of the Agasthyamala Biosphere Reserve forest, Western Ghats, Kerala, India","volume":"64","author":"Kumar","year":"2015","journal-title":"J. Med. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"819","DOI":"10.4269\/ajtmh.2005.72.819","article-title":"Localized cutaneous leishmaniasis due to Leishmania donovani and Leishmania tropica: Preliminary findings of the study of 161 new cases from a new endemic focus in himachal pradesh, India","volume":"72","author":"Sharma","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"289","DOI":"10.4269\/ajtmh.1989.41.289","article-title":"Visceral leishmaniasis unresponsive to pentostam caused by Leishmania tropica in Kenya","volume":"41","author":"Mebrahtu","year":"1989","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/0035-9203(93)90101-U","article-title":"Human cutaneous leishmaniasis caused by Leishmania donovani sl in Kenya","volume":"87","author":"Mebrahtu","year":"1993","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/S0035-9203(03)90061-7","article-title":"Sri Lankan cutaneous leishmaniasis is caused by Leishmania donovani zymodeme MON-37","volume":"97","author":"Karunaweera","year":"2003","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.pt.2009.07.002","article-title":"Leishmania donovani causing cutaneous leishmaniasis in Sri Lanka: A wolf in sheep\u2019s clothing?","volume":"25","author":"Karunaweera","year":"2009","journal-title":"Trends Parasitol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1179\/2047773212Y.0000000054","article-title":"Leishmania donovani zymodeme MON-37 isolated from an autochthonous visceral leishmaniasis patient in Sri Lanka","volume":"106","author":"Ranasinghe","year":"2012","journal-title":"Pathog. Glob. Health"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"476","DOI":"10.3201\/eid1303.060242","article-title":"Leishmania donovani and cutaneous leishmaniasis, Sri Lanka","volume":"13","author":"Siriwardana","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.trstmh.2007.10.005","article-title":"Identification of Leishmania donovani as a cause of cutaneous leishmaniasis in Sudan","volume":"102","author":"Elamin","year":"2008","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1186\/1752-1947-8-354","article-title":"A cluster of four cases of cutaneous leishmaniasis by Leishmania donovani in Cyprus: A case series","volume":"8","author":"Koliou","year":"2014","journal-title":"J. Med. Case Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1001\/archderm.134.2.193","article-title":"Cutaneous leishmaniasis due to Leishmania infantum: Case reports and literature review","volume":"134","author":"Marty","year":"1998","journal-title":"Arch. Dermatol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1056\/NEJM199305133281904","article-title":"Visceral infection caused by Leishmania tropica in veterans of Operation Desert Storm","volume":"328","author":"Magill","year":"1993","journal-title":"N. Engl. J. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1111\/ijd.13310","article-title":"Ongoing outbreak of cutaneous leishmaniasis in northwestern Yemen: Clinicoepidemiologic, geographic, and taxonomic study","volume":"55","author":"Khatri","year":"2016","journal-title":"Int. J. Dermatol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e15","DOI":"10.1016\/j.jaad.2009.04.047","article-title":"Cutaneous leishmaniasis in North-Western Yemen: A clinicoepidemiologic study and Leishmania species identification by polymerase chain reaction\u2013restriction fragment length polymorphism analysis","volume":"61","author":"Khatri","year":"2009","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.3201\/eid2608.191761","article-title":"Leishmania donovani infection with atypical cutaneous manifestations, Himachal Pradesh, India, 2014\u20132018","volume":"26","author":"Thakur","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1007\/s40257-022-00726-8","article-title":"Cutaneous leishmaniasis: A 2022 updated narrative review into diagnosis and management developments","volume":"23","author":"Schallig","year":"2022","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_25","unstructured":"World Health Organization Factsheets (2023, August 27). Diagnosis, Detection and Surveillance of Leishmaniasis. Available online: https:\/\/www.who.int\/teams\/control-of-neglected-tropical-diseases\/leishmaniasis\/diagnosis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"149","DOI":"10.4038\/cmj.v57i4.5082","article-title":"Correlation of clinical, parasitological and histopathological diagnosis of cutaneous leishmaniasis in an endemic region in Sri Lanka","volume":"57","author":"Ranawaka","year":"2013","journal-title":"Ceylon Med. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1179\/136485910X12647085215615","article-title":"Clinical and epidemiological studies on the cutaneous leishmaniasis caused by Leishmania (Leishmania) donovani in Sri Lanka","volume":"104","author":"Siriwardana","year":"2010","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1179\/136485908X300779","article-title":"Clinical manifestations of cutaneous leishmaniasis in Sri Lanka\u2014Possible evidence for genetic susceptibility among the Sinhalese","volume":"102","author":"Samaranayake","year":"2008","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.trstmh.2006.05.013","article-title":"Cutaneous leishmaniasis in southern Sri Lanka","volume":"101","author":"Rajapaksa","year":"2007","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.3201\/eid1307.060773","article-title":"Cutaneous leishmaniasis, Sri Lanka","volume":"13","author":"Nawaratna","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"10","DOI":"10.4038\/cmj.v48i1.3386","article-title":"Clinical features, risk factors and efficacy of cryotherapy in cutaneous leishmaniasis in Sri Lanka","volume":"48","author":"Siriwardana","year":"2003","journal-title":"Ceylon Med. J."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Yadav, P., Azam, M., Ramesh, V., and Singh, R. (2023). Unusual Observations in Leishmaniasis\u2014An Overview. Pathogens, 12.","DOI":"10.3390\/pathogens12020297"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"750","DOI":"10.12688\/f1000research.11120.1","article-title":"Leishmaniasis: A review","volume":"6","author":"Arenas","year":"2017","journal-title":"F1000Research"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1159\/000456543","article-title":"Clinical presentation of cutaneous leishmaniasis caused by Leishmania major","volume":"232","author":"Remadi","year":"2017","journal-title":"Dermatology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.4269\/ajtmh.2010.10-0143","article-title":"Cutaneous leishmaniasis (Leishmania major infection) in Dutch troops deployed in northern Afghanistan: Epidemiology, clinical aspects, and treatment","volume":"83","author":"Leenstra","year":"2010","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/j.jiph.2022.05.015","article-title":"Distribution and epidemiological features of cutaneous leishmaniasis in Asir Province, Saudi Arabia, from 2011 to 2020","volume":"15","author":"Alraey","year":"2022","journal-title":"J. Infect. Public Health"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.4269\/ajtmh.2010.10-0234","article-title":"Epidemiologic and clinical features of cutaneous leishmaniasis in southeastern Tunisia","volume":"83","author":"Bousslimi","year":"2010","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"777","DOI":"10.4269\/ajtmh.19-0531","article-title":"Clinical presentation and response to therapy in children with cutaneous leishmaniasis","volume":"102","author":"Suprien","year":"2020","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1111\/j.1365-4632.2008.03701.x","article-title":"Clinical features of cutaneous and disseminated cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Paraty, Rio de Janeiro","volume":"47","author":"Pirmez","year":"2008","journal-title":"Int. J. Dermatol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Guimaraes, L.H., Queiroz, A., Silva, J.A., Silva, S.C., Magalhaes, V., Lago, E.L., Machado, P.R., Bacellar, O., Wilson, M.E., and Beverley, S.M. (2016). Atypical manifestations of cutaneous leishmaniasis in a region endemic for Leishmania braziliensis: Clinical, immunological and parasitological aspects. PLoS Neglected Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0005100"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/j.1365-3024.2009.01116.x","article-title":"Immunopathogenic competences of Leishmania (V.) braziliensis and L.(L.) amazonensis in American cutaneous leishmaniasis","volume":"31","author":"Silveira","year":"2009","journal-title":"Parasite Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1590\/S0036-46652005000400003","article-title":"The histopathology of cutaneous leishmaniasis due to Leishmania (Leishmania) mexicana in the Yucatan peninsula, Mexico","volume":"47","year":"2005","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1590\/S0074-02762001000200005","article-title":"Clinical picture of cutaneous leishmaniases due to Leishmania (Leishmania) mexicana in the Yucatan peninsula, Mexico","volume":"96","year":"2001","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4538597","DOI":"10.1155\/2019\/4538597","article-title":"Leishmania donovani induced cutaneous leishmaniasis: An insight into atypical clinical variants in Sri Lanka","volume":"2019","author":"Siriwardana","year":"2019","journal-title":"J. Trop. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.clindermatol.2019.10.008","article-title":"Cutaneous leishmaniasis: A great imitator","volume":"38","author":"Gurel","year":"2020","journal-title":"Clin. Dermatol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.actatropica.2017.05.032","article-title":"Leishmaniasis in Turkey: Visceral and cutaneous leishmaniasis caused by Leishmania donovani in Turkey","volume":"173","author":"Harman","year":"2017","journal-title":"Acta Trop."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"235","DOI":"10.5858\/arpa.2013-0098-OA","article-title":"Comparison of conventional, molecular, and immunohistochemical methods in diagnosis of typical and atypical cutaneous leishmaniasis","volume":"138","author":"Shirian","year":"2014","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0140-6736(91)90734-7","article-title":"Leishmania donovani chagasi: New clinical variant of cutaneous leishmaniasis in Honduras","volume":"337","author":"Ponce","year":"1991","journal-title":"Lancet"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1179\/2047773215Y.0000000024","article-title":"Use of a clinical tool for screening and diagnosis of cutaneous leishmaniasis in Sri Lanka","volume":"109","author":"Siriwardana","year":"2015","journal-title":"Pathog. Glob. Health"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1017\/S003118201700169X","article-title":"Dermotropic Leishmania donovani in Sri Lanka: Visceralizing potential in clinical and preclinical studies","volume":"145","author":"Kariyawasam","year":"2018","journal-title":"Parasitology"},{"key":"ref_51","first-page":"90","article-title":"First report of mucosal tissue localisation of leishmaniasis in Sri Lanka","volume":"50","author":"Rajapaksa","year":"2005","journal-title":"Ceylon Med. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4038\/sljid.v2i2.4420","article-title":"Leishmaniasis in Sri Lanka: A decade old story","volume":"2","author":"Siriwardana","year":"2012","journal-title":"Sri Lankan J. Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e12413","DOI":"10.1111\/pim.12413","article-title":"In situ immunopathological changes in cutaneous leishmaniasis due to Leishmania donovani","volume":"39","author":"Manamperi","year":"2017","journal-title":"Parasite Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Wijesinghe, H., Gunathilaka, N., Semege, S., Pathirana, N., Manamperi, N., de Silva, C., and Fernando, D. (2020). Histopathology of cutaneous leishmaniasis caused by Leishmania donovani in Sri Lanka. BioMed Res. Int., 2020.","DOI":"10.1155\/2020\/4926819"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Reim\u00e3o, J.Q., Coser, E.M., Lee, M.R., and Coelho, A.C. (2020). Laboratory diagnosis of cutaneous and visceral leishmaniasis: Current and future methods. Microorganisms, 8.","DOI":"10.3390\/microorganisms8111632"},{"key":"ref_56","first-page":"148","article-title":"Atypical mucocutaneous involvement with Leishmania donovani","volume":"25","author":"Pulimood","year":"2012","journal-title":"Natl. Med. J. India"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"895","DOI":"10.4269\/ajtmh.13-0582","article-title":"Comparison of clinical samples and methods in chronic cutaneous leishmaniasis","volume":"91","author":"Eroglu","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1179\/2047773215Y.0000000032","article-title":"Characterisation of cutaneous leishmaniasis in Matara district, southern Sri Lanka: Evidence for case clustering","volume":"109","author":"Kariyawasam","year":"2015","journal-title":"Pathog. Glob. Health"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2632010X221134804","DOI":"10.1177\/2632010X221134804","article-title":"Immunopathology of Cutaneous Leishmaniasis in a Cohort of Sri Lankan Patients","volume":"15","author":"Wijesinghe","year":"2022","journal-title":"Clin. Pathol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"759","DOI":"10.4269\/ajtmh.17-0748","article-title":"Tissue impression smears as a supplementary diagnostic method for histopathology in cutaneous leishmaniasis in Sri Lanka","volume":"98","author":"Manamperi","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"106979","DOI":"10.1016\/j.actatropica.2023.106979","article-title":"First evidence of experimental genetic hybridization between cutaneous and visceral strains of Leishmania donovani within its natural vector Phlebotomus argentipes","volume":"245","author":"Riyal","year":"2023","journal-title":"Acta Trop."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"106","DOI":"10.4038\/cmj.v55i4.2626","article-title":"A histopathological study of cutaneous leishmaniasis in Sri Lanka","volume":"55","author":"Herath","year":"2010","journal-title":"Ceylon Med. J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.3402\/gha.v2i0.2046","article-title":"Low-cost liquid medium for in vitro cultivation of Leishmania parasites in low-income countries","volume":"2","author":"Tasew","year":"2009","journal-title":"Glob. Health Action"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"58","DOI":"10.4038\/cmj.v47i2.3454","article-title":"First successful in vitro culture of autochthonous Leishmania sp. in Sri Lanka","volume":"47","author":"Ihalamulla","year":"2002","journal-title":"Ceylon Med. J."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"179","DOI":"10.4038\/cmj.v56i4.3904","article-title":"First successful in vitro culture of Leishmania sp. causing autochthonous visceral leishmaniasis in Sri Lanka","volume":"56","author":"Ranasinghe","year":"2011","journal-title":"Ceylon Med. J."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"46","DOI":"10.4038\/cmj.v54i2.864","article-title":"A simple, cost effective method for isolation and transportation of Leishmania parasites","volume":"54","author":"Ihalamulla","year":"2009","journal-title":"Ceylon Med. J."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"294","DOI":"10.4269\/ajtmh.2004.70.294","article-title":"A sensitive new microculture method for diagnosis of cutaneous leishmaniasis","volume":"70","author":"Allahverdiyev","year":"2004","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1179\/136485905X51364","article-title":"Microculture for the isolation of Leishmania parasites from cutaneous lesions\u2014Sri Lankan experience","volume":"99","author":"Ihalamulla","year":"2005","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1111\/cup.13032","article-title":"Histopathologic features of cutaneous leishmaniasis and use of CD1a staining for amastigotes in Old World and New World leishmaniasis","volume":"44","author":"Sundharkrishnan","year":"2017","journal-title":"J. Cutan. Pathol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1080\/20477724.2021.1896265","article-title":"Diagnosing human cutaneous leishmaniasis using fluorescence in situ hybridization","volume":"115","author":"Wickremasinghe","year":"2021","journal-title":"Pathog. Glob. Health"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1017\/S0031182017002013","article-title":"High-throughput sequencing of kDNA amplicons for the analysis of Leishmania minicircles and identification of Neotropical species","volume":"145","author":"Kocher","year":"2018","journal-title":"Parasitology"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1590\/0074-02760150286","article-title":"Polymerase chain reaction detection of LeishmaniaDNA in skin biopsy samples in Sri Lanka where the causative agent of cutaneous leishmaniasis is Leishmania donovani","volume":"110","author":"Ranasinghe","year":"2015","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Deepachandi, B., Weerasinghe, S., Soysa, P., Karunaweera, N., and Siriwardana, Y. (2019). A highly sensitive modified nested PCR to enhance case detection in leishmaniasis. BMC Infect. Dis., 19.","DOI":"10.1186\/s12879-019-4180-3"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1590\/S0074-02762012000500014","article-title":"Development and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identificatio nof the parasite species","volume":"107","author":"Volpini","year":"2012","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"De Silva, N.L., De Silva, V.N., Deerasinghe, A.T., Rathnapala, U.L., Itoh, M., Takagi, H., Weerasooriya, M.V., Kato, H., and Yahathugoda, T.C. (2022). Development of a highly sensitive nested PCR and its application for the diagnosis of cutaneous leishmaniasis in Sri Lanka. Microorganisms, 10.","DOI":"10.3390\/microorganisms10050990"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0035-9203(01)90173-7","article-title":"Genetic heterogeneity in the species Leishmania tropica revealed by different PCR-based methods","volume":"95","author":"Schnur","year":"2001","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"12329","DOI":"10.1038\/s41598-023-37458-6","article-title":"Leishmania donovani persistence and transmission causing cutaneous leishmaniasis in unusual-foci of Nepal","volume":"13","author":"Rai","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"45","DOI":"10.4103\/ijdvl.IJDVL_14_18","article-title":"Identification of atypical dermal leishmaniasis resolved by restriction fragment length polymorphism","volume":"86","author":"Moulik","year":"2020","journal-title":"Indian J. Dermatol. Venereol. Leprol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"e63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"68","DOI":"10.4038\/cmj.v61i2.8286","article-title":"Loop-mediated isothermal amplification assay as a sensitive diagnostic tool for Leishmania donovani infections in Sri Lanka","volume":"61","author":"Kothalawala","year":"2016","journal-title":"Ceylon Med. J."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1186\/s13071-018-3238-1","article-title":"Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka","volume":"11","author":"Gunaratna","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1155\/1990\/960793","article-title":"Multilocus enzyme electrophoresis\u2013application to the study of meningococcal meningitis and listeriosis","volume":"1","author":"Ashton","year":"1990","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.micinf.2009.04.009","article-title":"The paraphyletic composition of Leishmania donovani zymodeme MON-37 revealed by multilocus microsatellite typing","volume":"11","author":"Alam","year":"2009","journal-title":"Microbes Infect."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1586\/eri.12.148","article-title":"Developments in diagnosis and treatment of visceral leishmaniasis during the last decade and future prospects","volume":"11","author":"Ejazi","year":"2013","journal-title":"Expert Rev. Anti Infect. Ther."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"24","DOI":"10.4269\/ajtmh.16-84256","article-title":"Diagnosis and treatment of leishmaniasis: Clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH)","volume":"96","author":"Aronson","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"843","DOI":"10.4269\/ajtmh.2008.79.843","article-title":"Positive rK39 serologic assay results in US servicemen with cutaneous leishmaniasis","volume":"79","author":"Hartzell","year":"2008","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.ijpara.2008.06.016","article-title":"Cutaneous leishmaniasis caused by Leishmania infantum transmitted by Phlebotomus tobbi","volume":"39","author":"Alten","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"De Silva, N.L., De Silva, V.N., Deerasinghe, A.T., Rathnapala, U.L., Kato, H., Itoh, M., Takagi, H., Weerasooriya, M.V., and Yahathugoda, T.C. (2022). Validation of an In-House ELISA Method in the Diagnosis of Cutaneous Leishmaniasis Caused by Leishmania donovani in Hambantota District, Sri Lanka. Microorganisms, 10.","DOI":"10.3390\/microorganisms10050921"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Bracamonte, M.E., \u00c1lvarez, A.M., Sosa, A.M., Hoyos, C.L., Lauthier, J.J., Cajal, S.P., Juarez, M., Uncos, R.E., S\u00e1nchez-Vald\u00e9z, F.J., and Acu\u00f1a, L. (2020). High performance of an enzyme linked immunosorbent assay for American tegumentary leishmaniasis diagnosis with Leishmania (Viannia) braziliensis amastigotes membrane crude antigens. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0232829"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Siriwardana, Y.D., Deepachandi, B., Ranasinghe, S., Soysa, P., and Karunaweera, N. (2018). Evidence for seroprevalence in human localized cutaneous Leishmaniasis caused by Leishmania donovani in Sri Lanka. BioMed Res. Int., 2018.","DOI":"10.1155\/2018\/9320367"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"9289651","DOI":"10.1155\/2020\/9289651","article-title":"Prevalidation of an ELISA for Detection of a New Clinical Entity: Leishmania donovani-Induced Cutaneous Leishmaniasis","volume":"2020","author":"Deepachandi","year":"2020","journal-title":"Int. J. Anal. Chem."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"e12940","DOI":"10.1111\/pim.12940","article-title":"ELISA-based evaluation of antibody response to Leishmania in a region endemic for cutaneous leishmaniasis","volume":"44","author":"Piyasiri","year":"2022","journal-title":"Parasite Immunol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1590\/S0074-02762013000300006","article-title":"Specificity of the rapid rK39 antigen-based immunochromatographic test Kalazar Detect (r) in patients with cutaneous leishmaniasis in Brazil","volume":"108","author":"Molinet","year":"2013","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"9932","DOI":"10.1038\/s41598-019-46283-9","article-title":"A multicentric evaluation of dipstick test for serodiagnosis of visceral leishmaniasis in India, Nepal, Sri Lanka, Brazil, Ethiopia and Spain","volume":"9","author":"Ejazi","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Van Henten, S., Fikre, H., Melkamu, R., Dessie, D., Mekonnen, T., Kassa, M., Bogale, T., Mohammed, R., Cnops, L., and Vogt, F. (2022). Evaluation of the CL detect rapid test in Ethiopian patients suspected for cutaneous leishmaniasis. PLoS Neglected Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010143"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"716","DOI":"10.4269\/ajtmh.18-0066","article-title":"Accuracy of a rapid diagnostic test based on antigen detection for the diagnosis of cutaneous leishmaniasis in patients with suggestive skin lesions in Morocco","volume":"99","author":"Bennis","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"De Silva, G., Somaratne, V., Senaratne, S., Vipuladasa, M., Wickremasinghe, R., Wickremasinghe, R., and Ranasinghe, S. (2017). Efficacy of a new rapid diagnostic test kit to diagnose Sri Lankan cutaneous leishmaniasis caused by Leishmania donovani. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0187024"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Adams, E.R., Jacquet, D., Schoone, G., Gidwani, K., Boelaert, M., and Cunningham, J. (2012). Leishmaniasis direct agglutination test: Using pictorials as training materials to reduce inter-reader variability and improve accuracy. PLoS Neglected Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001946"},{"key":"ref_99","first-page":"260","article-title":"Cutaneous leishmaniasis","volume":"18","author":"Ahmed","year":"2009","journal-title":"Mymensingh Med. J. MMJ"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"e193","DOI":"10.1111\/jdv.16111","article-title":"Ultrasonographic characteristics of cutaneous leishmaniasis","volume":"34","author":"Saavedra","year":"2020","journal-title":"J. Eur. Acad. Dermatol. Venereol. JEADV"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Zare, M., Akbarialiabad, H., Parsaei, H., Asgari, Q., Alinejad, A., Bahreini, M.S., Hosseini, S.H., Ghofrani-Jahromi, M., Shahriarirad, R., and Amirmoezzi, Y. (2022). A machine learning-based system for detecting leishmaniasis in microscopic images. BMC Infect. Dis., 22.","DOI":"10.1186\/s12879-022-07029-7"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"e15699","DOI":"10.1111\/dth.15699","article-title":"Biophysical and ultrasonographic changes of acute Old World cutaneous leishmaniasis skin lesions in comparison with uninvolved skin: A possible tool for non-invasive early detection and treatment outcome assessment","volume":"35","author":"Khatami","year":"2022","journal-title":"Dermatol. Ther."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"4208254","DOI":"10.1155\/2021\/4208254","article-title":"Image Processing for mHealth-Based Approach to Detect the Local Tissue Inflammation in Cutaneous Leishmaniasis: A Proof of Concept Study","volume":"2021","author":"Silva","year":"2021","journal-title":"Comput. Math. Methods Med."},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Due\u00f1as, E., Nakamoto, J.A., Cabrera-Sosa, L., Huaihua, P., Cruz, M., Ar\u00e9valo, J., Mil\u00f3n, P., and Adaui, V. (2022). Novel CRISPR-based detection of Leishmania species. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.958693"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ijid.2021.11.029","article-title":"Amplicon-based next-generation sequencing reveals the co-existence of multiple Leishmania species in patients with visceral leishmaniasis","volume":"115","author":"Ayala","year":"2022","journal-title":"Int. J. Infect. Dis."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"e00652-21","DOI":"10.1128\/Spectrum.00652-21","article-title":"Development of an amplicon-based next-generation sequencing protocol to identify Leishmania species and other trypanosomatids in leishmaniasis endemic areas","volume":"9","author":"Jaimes","year":"2021","journal-title":"Microbiol. Spectr."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1186\/s13071-023-05878-y","article-title":"High-resolution melting (HRM)-based detection of polymorphisms in the malic enzyme and glucose-6-phosphate isomerase genes for Leishmania infantum genotyping","volume":"16","author":"Buffi","year":"2023","journal-title":"Parasites Vectors"}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/13\/18\/2989\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:53:13Z","timestamp":1760129593000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/13\/18\/2989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,18]]},"references-count":107,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["diagnostics13182989"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics13182989","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,18]]}}}